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Sequence±structure±function studies of tRNA
Sequence±structure±function studies of tRNA

... Methylation of nucleic acids is catalyzed by a large and diverse class of S-adenosyl-L-methionine (AdoMet)-dependent methyltransferases. The enzymes characterized to date include members of two unrelated superfamilies: `classical' Rossmann-fold-like (1,2) and SPOUT (3). The relatively small SPOUT su ...
The polymorphism in MUC1 gene in Nelore cattle
The polymorphism in MUC1 gene in Nelore cattle

... of the uterine epithelial tissue with predicted functions in protection and cell–cell adhesion. These properties are closely related with the repetitive region [variable number of tandem repeats (VNTR)] of the extracellullar domain and with the O-glycosylation in their serine and threonine residues. ...
Identification of markers tightly linked to tomato yellow
Identification of markers tightly linked to tomato yellow

... mi/mi homozygous plants with only a 750-bp band. We additionally developed a multiplex PCR technique for JB-1 and Mi, which confer resistance to tomato yellow leaf curl disease and root-knot nematode. The JB-1 marker identified the genotype of the Ty gene, and the plants Genetics and Molecular Resea ...
Differential chromatin packaging of genomic
Differential chromatin packaging of genomic

... and mouse loci including the c-Ha-Ras-1 gene (30–32). Variation in the methylation of allelic sites is tissue specific and reproducible after transmission through the germ line. A putative cis-acting element(s) must be close to the Zfp127 locus to explain the complete cosegregation observed in seven ...
MOD ODN - rci.rutgers.edu
MOD ODN - rci.rutgers.edu

20. Transposable Genetic Elements
20. Transposable Genetic Elements

Forensics Test Key
Forensics Test Key

... What is controlled to switch between the various processes above? What is the name of the enzyme used in this process? Name the four nucleotides that are found in DNA What does DNA stand for? ...
The physics behind the larger scale organization of DNA in eukaryotes
The physics behind the larger scale organization of DNA in eukaryotes

... length without linker histones do not condense into a 33 nm fiber, but into a less dense structure. Pulling experiments in the pico-Newton range [8] suggest nonetheless that at low forces, such fibers have the same force–extension behavior with or without linker histones. Fibers without linker histo ...
Lack of homology between two haloacetate dehalogenase genes
Lack of homology between two haloacetate dehalogenase genes

... amino acid composition and molecular mass of the dehH2 gene product corresponded closely to those of the H-2 enzyme (Table 1). The dehH2 gene was preceded by the sequence GGAGA, the putative Shine-Dalgarno sequence, present 6 bp upstream from the ATG initiation codon. A promoter that initiates trans ...
GENETICS TEST II - Daytona State College
GENETICS TEST II - Daytona State College

... • A double-stranded nucleic acid molecule in which each polynucleotide chain has a different origin. It may be produced as an intermediate in a recombinational event or by the in vitro reannealing of single-stranded, complementary molecules. • In transformation, once the extracellular DNA is integra ...
FLUORESCENT PROTEIN IN Escherichia coli
FLUORESCENT PROTEIN IN Escherichia coli

En/Spm-LIKE TRANSPOSONS IN POACEAE SPECIES
En/Spm-LIKE TRANSPOSONS IN POACEAE SPECIES

... a given cereal’s nuclear genome) [1]. From the investigation of the Drosophila genomes, it is known that TE can give rise to significant genomic changes by creating mutations, altering gene expression, conducting telomeric function, and promoting chromosomal aberrations [2-4]. TE was divided into tw ...
Antimicrobial Agents and Chemotherapy
Antimicrobial Agents and Chemotherapy

... double label. (b) Sedimentation analysis of RApOI plasmid in neutral sucrose gradient (5 to 20%). Symbols: (-) RApOI (13H]thymidine labeled); (0) T169 (['4C]thymidine labeled). ...
Genetics - Michael
Genetics - Michael

... of techniques, scientists are now able to extract DNA from our extinct relatives, including the Neanderthals. By comparing this DNA with our own and our close primate relatives, geneticists are beginning to zero in on those genes which enable cognitive thoughts and physiological changes known to be ...
Genome organization of Magnaporthe grisea
Genome organization of Magnaporthe grisea

... 61 random ascospore progeny were used to determine the segregation of RFLPs as described by Skinner et al. (1993). Genomic DNA isolation Mycelia from oatmeal plate cultures of M. grisea were used to inoculate 50 ml of liquid complete medium (CM) in 250-ml Erlenmeyer flasks. The flasks were incubated ...
The Large Loop Repair and Mismatch Repair Pathways
The Large Loop Repair and Mismatch Repair Pathways

... mitotic growth. NER functions to repair bulky DNA lesions, such as thymine dimers and other helix-distorting lesions. During NER the damaged nucleotide is recognized and bound by several NER proteins, and the DNA surrounding the lesion is unwound. The single-stranded DNA containing the lesion is rem ...
Life 9e - Garvness
Life 9e - Garvness

... c. translocations involve the breakage and insertion of DNA segments in reverse order, whereas duplications are the breakage at different points on the chromosome. d. duplications lead to duplications of the chromosome, whereas translocations involve swapping of chromosome segments. e. translocation ...
Ends-out, or replacement, gene targeting in Drosophila
Ends-out, or replacement, gene targeting in Drosophila

... the w⫹ offspring (10 of 1,154 from 22 vials counted ⫽ 0.9% of all w⫹ progeny), although we expected them to be quite frequent as a result of excision and loss of y⫹. One possible explanation supposes that repair of the cut chromosomal ends is very inefficient, and although targeting may have occurre ...
P003 MLH1 / MSH2 EXON DELETION TEST KIT:
P003 MLH1 / MSH2 EXON DELETION TEST KIT:

... The PM200 Mouse control probemix contains 6 different probes with amplification products between 175 and 545 nt, as well as 4 control fragments generating an amplification product smaller than 90 nt. In many cases, resul;ts can be confirmed. Southern blot, FISH or long range PCR. The length of the a ...
DNA Specificity of the Bicoid Activator Protein Is Determined by
DNA Specificity of the Bicoid Activator Protein Is Determined by

... Bicoid fusion proteins activated target genes that carried upstream LexA operators, GAL4 sites, or Bicoid binding sites. We used the gene activation phenotype to establish which residues in the Bicoid homeodomain are required for DNA recognition. Unlike bacterial helix-turn-helix proteins, amino aci ...
Chapter 1: An Introduction to Chemistry
Chapter 1: An Introduction to Chemistry

... Classify as reversible or irreversible the effects of (a) hot water on the proteins of gelatin, (b) the high temperature canning process on the proteins of bromelin, and (c) the high temperatures of frying on the proteins of egg white. ...
FastStart Universal SYBR Green Master (ROX)
FastStart Universal SYBR Green Master (ROX)

Application for rDNA Review/Registration for
Application for rDNA Review/Registration for

... have designed the experiments to minimize potential hazards, and have communicated potential hazards to others who may come in contact with the products you propose to use or generate. This form is designed to encompass many different biosafety concerns the IBC registers, reviews, and approves for t ...
SNP
SNP

... they are in physical proximity and one fluorophore’s emission spectrum (the donor) overlaps the other’s (the acceptor) excitation spectrum. When FRET occurs the donor emission is quenched and the acceptor emission increases when the donor is excited FRET can be monitored by quenching of donor emissi ...
Nursing Scientific Meeting World Congress of Dermatology
Nursing Scientific Meeting World Congress of Dermatology

... nurses at low cost. ISNG also supports national groups since, as is often the case, dermatology nurses are isolated in many countries without any national membership organisation. It promotes educational opportunities at often established international events that raise the profile of dermatology nu ...
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DNA supercoil



DNA supercoiling refers to the over- or under-winding of a DNA strand, and is an expression of the strain on that strand. Supercoiling is important in a number of biological processes, such as compacting DNA. Additionally, certain enzymes such as topoisomerases are able to change DNA topology to facilitate functions such as DNA replication or transcription. Mathematical expressions are used to describe supercoiling by comparing different coiled states to relaxed B-form DNA.As a general rule, the DNA of most organisms is negatively supercoiled.
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