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Figure 15.6 Nonreplicative transposition allows a transposon to
Figure 15.6 Nonreplicative transposition allows a transposon to

... Insertion sequences are simple transposition modules Composite transposons have IS modules Transposition occurs by both replicative and nonreplicative mechanisms 15.5 Transposons cause rearrangement of DNA 15.6 Common intermediates for transposition 15.7 Replicative transposition proceeds through a ...
15.13 Spm elements influence gene expression
15.13 Spm elements influence gene expression

... Insertion sequences are simple transposition modules Composite transposons have IS modules Transposition occurs by both replicative and nonreplicative mechanisms 15.5 Transposons cause rearrangement of DNA 15.6 Common intermediates for transposition 15.7 Replicative transposition proceeds through a ...
pyrimidine
pyrimidine

... That’s one of the technologies that has made large-scale sequencing projects like the human genome project possible ...
Facts about the mini-Tn7 transposon system as a tool for
Facts about the mini-Tn7 transposon system as a tool for

... sequences located on the delivery plasmid between the transposon ends, Tn7L and Tn7R (see Fig. 2). The delivery plasmid is some times also named the carrier plasmid. Delivery plasmids The delivery plasmids presented here are all pUC19 derivatives, which can replicate in E. coli and other Enterics, b ...
PDF
PDF

... None of these compounds caused any alterations of polarity. At non-toxic concentrations reconstitution was exclusively monopolar in form, even in isolated digestive zones. At lethal doses animals were axiate with easily recognizable polarity right up to, and during, disintegration; invariably the la ...
The Arabidopsis RAD51 paralogs RAD51B, RAD51D and XRCC2
The Arabidopsis RAD51 paralogs RAD51B, RAD51D and XRCC2

... RAD51 family tree (Lin et al., 2006). Therefore, these genes might have overlapping/redundant functions in plant mitotic cell cycle or meiotic cells. It is also possible that RAD51B, RAD51D and XRCC2 are not required for normal meiosis, even when their functions are lost simultaneously. Nevertheless ...
(S) tet Resistance Determinant Element Containing the Tetracycline
(S) tet Resistance Determinant Element Containing the Tetracycline

Fulltext PDF - Indian Academy of Sciences
Fulltext PDF - Indian Academy of Sciences

... on DNA sequence. The latter is in the form of cytosine methylation at CG, CHG and CHH elements (where H = A, T or C) and a variety of histone modifications in nucleosomes. The epialleles arising from cytosine methylation marks on the nuclear genomic loci have better heritability than the epiallelic v ...
Phenotypic Stability of trp Operon Recombinant
Phenotypic Stability of trp Operon Recombinant

... pSC101-trp and RSF2124-trp were originally in E. coli C600-1. Plasmid preparatiorr. Escherichia coli carrying RSF2124-trp was grown ovei night in PBB and diluted 10fold in 100 ml M-9 medium (Clowes & Hayes, 1968) supplemented with Casamino acids (20 g 1-lj and thiamin (1Opg ml-l) or thymine (1Opg ml ...
Nicolaus Copernicus` DNA found in Uppsala
Nicolaus Copernicus` DNA found in Uppsala

... and responsible for the collection of old instruments at the Observatory. I have been retired since the spring of 2008. In 1973, during the celebration of the 500 year anniversary of the birth of Copernicus, Peter asked me to assist him with his Copernicus’ exhibition at the City Library in Uppsala ...
PDF
PDF

... et al., 2011) identified Thermotoga species as the closest relatives to Dictyoglomus. ANI values were generated using the D. thermophilum genome, eight finished, closed Thermotoga genomes and three finished, closed Caldicellulosiruptor genomes. ANI values (Kim et al., 2014) were computed as pairwise ...
Introduction to Molecular Diagnostics
Introduction to Molecular Diagnostics

Molecular Evidence for Vector Implication of Onchocerca lupi in Los
Molecular Evidence for Vector Implication of Onchocerca lupi in Los

... The nodules are primarily composed of eosinophils, histiocytes, fibroblasts, and newly formed blood vessels (Zarfoss, Dubielzig, Eberhard, & Schmidt, 2005). This is typically associated with exophthalmos and possible third-eyelid protrusion (Komnenou, Egyed, Sréter, & Eberhard, 2003). In almost all ...
Generation and analysis of mutated clonal scFv Jiya George
Generation and analysis of mutated clonal scFv Jiya George

... Human immuno deficiency virus (HIV) incorporates host cellular protein, beta-2-microglobulin (β2m), into its surface envelope during budding. β2m is a cellular protein that belongs to the major histocompatibility complex (MHC) Class I molecules. Studies have shown anti-β2m monoclonal antibodies (mAb ...
Mutations
Mutations

... • Inversion 1800 rotation of piece of DNA • Reciprocal translocation – parts of nonhomologous chromosomes change ...
Sequence analysis of 16S rRNA, gyrB and catA genes and DNA
Sequence analysis of 16S rRNA, gyrB and catA genes and DNA

... (GenBank accession numbers are given in parentheses): Rhodococcus sp. BBG1 (HE820128), Rhodococcus sp. RGN4 (HE801274), Rhodococcus sp. K5 (KF790905), Rhodococcus sp. PT2-14B (KF360060), Rhodococcus sp. PT3-14 (KF360061) and Rhodococcus sp. Ba49 (KF360059). All strains were maintained on DSMZ medium ...
DNA Base Composition, DNA-DNA Homology and Long
DNA Base Composition, DNA-DNA Homology and Long

... closely related fatty acid profiles within S. salivarius and S . therrnophilus further supports the close relatedness between these taxa as shown by DNA-DNA homology studies (Kilpper-Balz et al., 1982; Table 2). Although S. salivarius and S . thermophilus are physiologically and biochemically very d ...
DNA Base Composition, DNA-DNA Homology and Long
DNA Base Composition, DNA-DNA Homology and Long

... closely related fatty acid profiles within S. salivarius and S . therrnophilus further supports the close relatedness between these taxa as shown by DNA-DNA homology studies (Kilpper-Balz et al., 1982; Table 2). Although S. salivarius and S . thermophilus are physiologically and biochemically very d ...
Sequence analysis of 16S rRNA, gyrB and catA genes and DNA
Sequence analysis of 16S rRNA, gyrB and catA genes and DNA

... (GenBank accession numbers are given in parentheses): Rhodococcus sp. BBG1 (HE820128), Rhodococcus sp. RGN4 (HE801274), Rhodococcus sp. K5 (KF790905), Rhodococcus sp. PT2-14B (KF360060), Rhodococcus sp. PT3-14 (KF360061) and Rhodococcus sp. Ba49 (KF360059). All strains were maintained on DSMZ medium ...
Extending the Implications of Myriad to Ambry â•fiThe New
Extending the Implications of Myriad to Ambry â•fiThe New

... DNA stores massive amounts of information within it using a complex coding system which utilizes 4 chemical bases that have unique pairing abilities and restrictions: 1) Adenine (A), 2) Thymine (T), 3) Guanine (G), and 4) Cytosine (C). The sequence of these bases results in different outcomes for bo ...
Lack of expression of XIST from a small ring X chromosome
Lack of expression of XIST from a small ring X chromosome

... nonrandom X-chromosome inactivation, digestion with HhaI resulted in loss of only one allele, that from the active X chromosome (Figure 3B). The ZXDA gene in Xp11 contains a polymorphic CA repeat in the transcribed but untranslated region of the gene. This gene is subject to X-chromosome inactivatio ...
How to obtain and recognize partial-diploid strains that are duplicated... chromosome segments.
How to obtain and recognize partial-diploid strains that are duplicated... chromosome segments.

... (This is especially useful with unstable duplications that break down to give fertile heterokaryons). If a duplication-generating strain that carries a recessive marker located in the translocated segment is crossed with a normal-sequence strain carrying a different recessive marker in the correspon ...
Specific oligonucleotide primers for detection of endoglucanase
Specific oligonucleotide primers for detection of endoglucanase

Gene Section RAD52 (RAD52 homolog (S. cerevisiae)) Atlas of Genetics and Cytogenetics
Gene Section RAD52 (RAD52 homolog (S. cerevisiae)) Atlas of Genetics and Cytogenetics

... and hRAD52 with replication protein A (RPA) is important for the binding with ssDNA by RAD52 (Hays et al., 1998; Shinohara et al., 1998; Jackson et al., 2002). hRAD52 binds directly to DSBs, protects them from exonuclease resection, and facilitates end-to-end interaction (Van Dyck et al., 1999). Fur ...
Nanopore Unzipping of Individual DNA Hairpin Molecules
Nanopore Unzipping of Individual DNA Hairpin Molecules

... duplex region sequence, and that the intercept depends strongly on the duplex region energy. We also present the first nanopore dynamic force measurements (time varying force). Our results are in agreement with the ~log(V_ ) dependence at high V_ (where V_ is the loading rate) observed by other metho ...
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DNA supercoil



DNA supercoiling refers to the over- or under-winding of a DNA strand, and is an expression of the strain on that strand. Supercoiling is important in a number of biological processes, such as compacting DNA. Additionally, certain enzymes such as topoisomerases are able to change DNA topology to facilitate functions such as DNA replication or transcription. Mathematical expressions are used to describe supercoiling by comparing different coiled states to relaxed B-form DNA.As a general rule, the DNA of most organisms is negatively supercoiled.
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