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Wide-spread polyploidizations during plant evolution Dicot
Wide-spread polyploidizations during plant evolution Dicot

... CDEI I I consensus is 26 bp. Within a given species there are often further invariant sites in their CDEI or CDEI I I regions, for example G at positions 2 and 8 in S. cerevisiae CDEI I I . T he ...
Painting the target around the matching profile
Painting the target around the matching profile

... certain that all alleles of all contributors have been detected, then the number of possible contributor genotypes is even greater. To avoid false exclusions, analysts must make allowances for these phenomena when determining whether a suspect should be ‘included’ or ‘excluded’ as a potential contri ...
A helicoidal transfer matrix model for inhomogeneous DNA melting
A helicoidal transfer matrix model for inhomogeneous DNA melting

Strategies for Attaching Oligonucleotides to Solid Supports
Strategies for Attaching Oligonucleotides to Solid Supports

... Polystyrene micro-spheres can be fluorescently labeled through internal dye entrapment or surface attachment [11]. Surface labeling is preferred if the environmental responsiveness of the dye is important or if the particles are to be used in a nonaqueous solvent. In most applications, however, inte ...
BIO450 Primer Design Tutorial
BIO450 Primer Design Tutorial

... The annealing temperature for a PCR reaction is based on the melting temperature (Tm) of the primers bound to the templates. The Tm is the temperature at which a population of double stranded DNA molecules has half of the molecules in a single stranded state and half in the double stranded state. At ...
Structure and functions of lampbrush chromosomes
Structure and functions of lampbrush chromosomes

... subject to transcription at the time. Although in the case of physical factors, such as radiation or numerous chemical factors, a similar effect on the structure and activity of LBCs in various groups of animals can be expected, seasonal changes predominantly affect polikilotherms (Morgan, 2002, 200 ...
Kein Folientitel
Kein Folientitel

... varieties; (b) essentially biological processes for the production of plants or animals. 2. Inventions which concern plants or animals shall be patentable if the technical feasibility of the invention is not confined to a particular plant or animal variety. 3. Paragraph 1(b) shall be without prejudi ...
Supplementary Materials and methods (doc 154K)
Supplementary Materials and methods (doc 154K)

... they are not properly assembled and plasmids are not annotated. We scanned 1866 genomes of free-living bacteria from the 31 different eubacteria phyla in the NCBI database. Endosymbiont species and obligate intracellular parasites were excluded due to their extremely reduced chromosomal and plasmid ...
Molecular analysis of putative genetic factors affecting BSE
Molecular analysis of putative genetic factors affecting BSE

... (see Table 1). To ensure that both alleles from the bull were transmitted, between 28 and 56 unaffected individuals from each family were also genotyped. These controls represented age and sex matched contemporaries from the farm from which cases were obtained. Inheritance of alleles from the sire w ...
Patient with syndromic cleft lip-palate, mosaic karyotype and
Patient with syndromic cleft lip-palate, mosaic karyotype and

... comes to several genes, but along with them morphogenesis could be controlled by many more genes (18). These genes are called “genetic background”, and they may participate in facial morphogenesis not directly. It could be about 20 such genes in different chromosome regions (6, 7). They alone make a ...
Recombinant DNA technology and molecular cloning
Recombinant DNA technology and molecular cloning

... The first cloning experiments Hamilton Smith and co-workers demonstrated unequivocally that restriction endoncleases cleave a specific DNA sequence. Later, Daniel Nathans used restriction endonucleases to map the simian virus 40 (SV40) genome and to locate the origin of replication. These major brea ...
Three-dimensional Structures of Bulge
Three-dimensional Structures of Bulge

... pioneering work of Fresco & Alberts (1960). The) introduced the notion that non-complementary residues in helical polynucleotides may be looped ...
Protein Synthesis
Protein Synthesis

...  Now you have found UCA on the chart.  Your chart may have the name of the amino acid written next to the codon “Serine”  Or it may have an abbreviation “ser”. If the abbreviation is there, then look that up in the listing of amino acids. “serine”  That is the name of the amino acid. Return to L ...
MayerFrankiPoster
MayerFrankiPoster

... photosynthetic process. Phycocyanin efficiently captures solar energy, but also prevents cyanobacterial cultures from growing to high densities because the cells closest to the surface absorb most of the sunlight and prevent the cells beneath from receiving sufficient light. This is known as ‘oversh ...
Exercise 8: Forensic Genetics/ Human Phenotypes
Exercise 8: Forensic Genetics/ Human Phenotypes

... DNA molecules move more slowly through the gel than smaller molecules, and compact molecules pass more quickly than less compact molecules (given they have the same molecular weight). In this exercise, you will load known and unknown samples onto a gel. You have 4 samples collected from a crime scen ...
Maternal uniparental disomyof chromosome 13
Maternal uniparental disomyof chromosome 13

... reported involving all or part of chromosomes homozygous despite all being highly (>75%) 6,7,11,14,15,16,21,22, and the XY pair.'4 polymorphic.'2 Such a chromosome could have These investigations are of value firstly for been formed by a translocation involving the determining which chromosomes cont ...
Collaborative coupling between polymerase and helicase for
Collaborative coupling between polymerase and helicase for

Solid Tumour Section Nervous system: Medulloblastoma Atlas of Genetics and Cytogenetics
Solid Tumour Section Nervous system: Medulloblastoma Atlas of Genetics and Cytogenetics

... breakpoint is in the proximal portion of p-arm at 17p11.2, so that the resultant structure is dicentric. In a few cases, partial or complete loss of 17p occurs through interstitial deletion, unbalanced translocation or monosomy 17. Chromosome 1 is also involved in medulloblastomas. The most frequent ...
Regional DNA Hypermethylation at D17S5
Regional DNA Hypermethylation at D17S5

p53 regulation and function in normal cells and tumors
p53 regulation and function in normal cells and tumors

... with transcription-independent induction of apoptosis. We suggest a model in which activated forms of p53 carry out transcription-dependent functions in growth arrest and DNA repair which may vary by cell type, while most or all wild type forms of p53 are capable of binding to damaged DNA. p53 prote ...
Chpt8_RecombineDNA.doc
Chpt8_RecombineDNA.doc

... the topic of this chapter. Transposition is a highly specialized form of recombination in which a segment of DNA moves from one location to another, either on the same chromosome or a different chromosome; this will be discussed in the next chapter. Types and examples of recombination At least four ...
Slide 1
Slide 1

... Note that this “reasoning” is performed using terms representing concepts rather than the concepts themselves. (Which is to say, text strings are being shuffled around; there is no “thought” involved.) “The computer doesn’t truly “understand” any of this information, but it can now manipulate the t ...
Jobling, M.A. - University of Leicester
Jobling, M.A. - University of Leicester

... male sex in mammals, including humans. The decision is made early in the life of the embryo, when the undifferentiated sex organs (gonads) have the potential to become either ovaries or testes. In the absence of a Y chromosome they follow their ‘default’ pathway, differentiating into ovaries, which ...
gene mutation -unit-2-study mat-2012
gene mutation -unit-2-study mat-2012

... environment. These mutagens include cosmic radiation, radioactive compounds, heat, and such naturally occuring base analogues like caffeine. These will be considered under ‘induced mutations’ as they are external agents bringing about mutations. Truly spontaneous mutations that will be dealt with he ...
Chapter 11 - Chromosome Mutations
Chapter 11 - Chromosome Mutations

... ANEUPLOIDS (missing part of a chromosome set) - generated through nondisjunction (chromosomes do not segregate at Meiosis I or chromatids do not separate at Meiosis II) (Figure 11-13) --------> n + 1 and n - 1 gametes Monosomic: only one copy of a chromosome is present ...
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DNA supercoil



DNA supercoiling refers to the over- or under-winding of a DNA strand, and is an expression of the strain on that strand. Supercoiling is important in a number of biological processes, such as compacting DNA. Additionally, certain enzymes such as topoisomerases are able to change DNA topology to facilitate functions such as DNA replication or transcription. Mathematical expressions are used to describe supercoiling by comparing different coiled states to relaxed B-form DNA.As a general rule, the DNA of most organisms is negatively supercoiled.
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