Defining characteristics of Tn5 Transposase non
... are attributed as the original place of publication with the correct citation details given; if an article is subsequently reproduced or disseminated not in its entirety but only in part or as a derivative work this must be clearly indicated. For commercial re-use, please contact journals.permission ...
... are attributed as the original place of publication with the correct citation details given; if an article is subsequently reproduced or disseminated not in its entirety but only in part or as a derivative work this must be clearly indicated. For commercial re-use, please contact journals.permission ...
Supplementary Materials and methods (doc 154K)
... 50% PAO1-GFP. Competitions starting at 50% of each strain maximize the ability to detect small differences in fitness. The exact initial proportions were confirmed via flow cytometry (see conditions below). Mixtures were diluted 200-fold in fresh LB and competed for 16 hours at 37°C with no agitatio ...
... 50% PAO1-GFP. Competitions starting at 50% of each strain maximize the ability to detect small differences in fitness. The exact initial proportions were confirmed via flow cytometry (see conditions below). Mixtures were diluted 200-fold in fresh LB and competed for 16 hours at 37°C with no agitatio ...
Oxalate decarboxylase of the white-rot fungus
... activities have been detected, secreted either to the culture medium or to the fungal cell wall and extracellular polysaccharide layer (Dutton et al., 1994; Kathiara et al., 2000). It is assumed that the major role of ODC in fungi is to prevent too high intracellular levels of oxalic acid, and there ...
... activities have been detected, secreted either to the culture medium or to the fungal cell wall and extracellular polysaccharide layer (Dutton et al., 1994; Kathiara et al., 2000). It is assumed that the major role of ODC in fungi is to prevent too high intracellular levels of oxalic acid, and there ...
Distribution of orphan metabolic activities
... reporting the sequenced gene for a new activity. Distribution of orphan activities across the biochemical space What is the distribution of orphan activities across the enzymatic functional space? One possibility is that orphan activities are primarily clustered in less studied regions of the EC cla ...
... reporting the sequenced gene for a new activity. Distribution of orphan activities across the biochemical space What is the distribution of orphan activities across the enzymatic functional space? One possibility is that orphan activities are primarily clustered in less studied regions of the EC cla ...
SALSA MLPA KIT ME003-A1 Tumor suppressor-3 - MRC
... silenced by methylation in tumours, but are unmethylated in blood-derived DNA of healthy individuals. In addition, 13 reference probes are included which are not affected by HhaI digestion. Besides detecting aberrant methylation, all 40 probes present will give information on copy number changes in ...
... silenced by methylation in tumours, but are unmethylated in blood-derived DNA of healthy individuals. In addition, 13 reference probes are included which are not affected by HhaI digestion. Besides detecting aberrant methylation, all 40 probes present will give information on copy number changes in ...
2008 HSC Notes from the Marking Centre Biology
... • look at the structure of the whole question and note that in some questions the parts follow from each other (ie responses in part (a) lead to the required response in part (b)). In Section II, the option question is divided into a number of parts. Candidates should clearly label each part of the ...
... • look at the structure of the whole question and note that in some questions the parts follow from each other (ie responses in part (a) lead to the required response in part (b)). In Section II, the option question is divided into a number of parts. Candidates should clearly label each part of the ...
Chapter 4 - DORAS
... Iron nutrition minimal media bioassays were used to analyse the utilisation of haemin by the transconjugants. A 1/50 dilution of E. coli overnight cultures, which had been grown in LB broth and the appropriate antibiotics, was used to inoculate 5 ml aliquots of M63 minimal media (section 2.2). These ...
... Iron nutrition minimal media bioassays were used to analyse the utilisation of haemin by the transconjugants. A 1/50 dilution of E. coli overnight cultures, which had been grown in LB broth and the appropriate antibiotics, was used to inoculate 5 ml aliquots of M63 minimal media (section 2.2). These ...
Genetic Mosaics and the Germ Line Lineage
... Recent sequencing studies using next-generation sequencing technology have documented that each individual’s genome contains at least 50–100 new point mutations that were not present in the genomes of either parent [25–31]. These are referred to as de novo mutations. Here, point mutations include si ...
... Recent sequencing studies using next-generation sequencing technology have documented that each individual’s genome contains at least 50–100 new point mutations that were not present in the genomes of either parent [25–31]. These are referred to as de novo mutations. Here, point mutations include si ...
Development and implementation of genomic predictions in beef cattle
... This occurred because of separate investments by Pfizer Animal Genetics and Merial Igenity in the development of reference populations. Due to the significant contribution of Angus cattle to the North American performance recorded population, Angus was an obvious choice for the initial investment. A ...
... This occurred because of separate investments by Pfizer Animal Genetics and Merial Igenity in the development of reference populations. Due to the significant contribution of Angus cattle to the North American performance recorded population, Angus was an obvious choice for the initial investment. A ...
24 Recombination Hotspots in Nonallelic Homologous Recombination
... the fine-mapping of all homologous recombination (HR) processes can only help our admittedly basic comprehension of what is a fundamental cellular process. In recent years there has been a growing desire to be able to use patterns of linkage disequilibrium (LD) throughout the genome to design more e ...
... the fine-mapping of all homologous recombination (HR) processes can only help our admittedly basic comprehension of what is a fundamental cellular process. In recent years there has been a growing desire to be able to use patterns of linkage disequilibrium (LD) throughout the genome to design more e ...
Consulta: subjectFacets:"16S rRNA" Registros recuperados: 43
... A reverse transcriptase - polymerase chain reaction based assay for Borrelia species detection in ticks was developed. The method was based on amplification of 552 nucleotide bases long sequence of 16S rRNA, targeted by Borrelia specific primers. In the present study, total RNA extracted from Ixodes ...
... A reverse transcriptase - polymerase chain reaction based assay for Borrelia species detection in ticks was developed. The method was based on amplification of 552 nucleotide bases long sequence of 16S rRNA, targeted by Borrelia specific primers. In the present study, total RNA extracted from Ixodes ...
Eds., N. Hamamura, S. Suzuki, S. Mendo, C. M. Barroso,... © by TERRAPUB, 2010.
... g·l–1 and 26°C, respectively. A preliminary experiment was performed to evaluate the influence of Av27 and TBT in the natural microbial community. Figure 3(A) shows that the samples containing TBT and inoculated with the resistant strain Av27 (1, 2, 3, 13, 14, 15) and without the Av27 strain (16, 17 ...
... g·l–1 and 26°C, respectively. A preliminary experiment was performed to evaluate the influence of Av27 and TBT in the natural microbial community. Figure 3(A) shows that the samples containing TBT and inoculated with the resistant strain Av27 (1, 2, 3, 13, 14, 15) and without the Av27 strain (16, 17 ...
Genome-wide scan of bipolar disorder in 65 pedigrees: supportive evidence for linkage at 8q24, 18q22, 4q32, 2p12, and 13q12.
... order was the most likely given the genotype data. For each of the chromosomes, we also built a map on the Marshfield web site using the ‘Build your own map’ function. Of the 842 markers in our data set, 142 were not in the Marshfield data set. There were 12 order discrepancies on seven chromosomes ...
... order was the most likely given the genotype data. For each of the chromosomes, we also built a map on the Marshfield web site using the ‘Build your own map’ function. Of the 842 markers in our data set, 142 were not in the Marshfield data set. There were 12 order discrepancies on seven chromosomes ...
wsp Gene Sequences from the Wolbachia of Filarial Nematodes
... and WSPintR from nematode Wolbachia were sequenced directly, and the sequences were aligned to the wsp gene available for arthropod Wolbachia. We also tried to align wsp to the gene sequences available for the major outer membrane proteins of Anaplasma, Ehrlichia, and Cowdria species. While alignmen ...
... and WSPintR from nematode Wolbachia were sequenced directly, and the sequences were aligned to the wsp gene available for arthropod Wolbachia. We also tried to align wsp to the gene sequences available for the major outer membrane proteins of Anaplasma, Ehrlichia, and Cowdria species. While alignmen ...
Construction of a genetic linkage map of Thlaspi
... (Ni) hyperaccumulator species, previously suggested to be a good model species in which to study the mechanisms of heavy metal hyperaccumulation (Assunção et al., 2003a). An important characteristic of T. caerulescens is its natural variation in important traits such as metal accumulation, metal roo ...
... (Ni) hyperaccumulator species, previously suggested to be a good model species in which to study the mechanisms of heavy metal hyperaccumulation (Assunção et al., 2003a). An important characteristic of T. caerulescens is its natural variation in important traits such as metal accumulation, metal roo ...
Genome-wide analysis of the distribution of AP2/ERF transcription
... Background: Cabbage (Brassica oleracea) is one of the most important leaf vegetables grown worldwide. The entire cabbage genome sequence and more than fifty thousand proteins have been obtained to date. However a high degree of sequence similarity and conserved genome structure remain between cabbag ...
... Background: Cabbage (Brassica oleracea) is one of the most important leaf vegetables grown worldwide. The entire cabbage genome sequence and more than fifty thousand proteins have been obtained to date. However a high degree of sequence similarity and conserved genome structure remain between cabbag ...
a nine-base pair deletion distinguishes two en/spm
... are examined as well as when nucleotide sequences are determined (BENNETZEN and RAMAKRISHNA, 2002; FU and DOONER 2003; SONG and MESSING, 2003; PENNISI, 2007). SCHWARZ-SOMMER et al. (1984) in an examination of the wx-m8 allele insert found two additional inserts, a smaller one, 250 bp, and a second o ...
... are examined as well as when nucleotide sequences are determined (BENNETZEN and RAMAKRISHNA, 2002; FU and DOONER 2003; SONG and MESSING, 2003; PENNISI, 2007). SCHWARZ-SOMMER et al. (1984) in an examination of the wx-m8 allele insert found two additional inserts, a smaller one, 250 bp, and a second o ...
Genit 3
... what is imp and all infos will be there hopefully So no need to go back to the slides only to see extra pics So if it seems long don’t be afraid ,mostly slides and pics for better understanding ...
... what is imp and all infos will be there hopefully So no need to go back to the slides only to see extra pics So if it seems long don’t be afraid ,mostly slides and pics for better understanding ...
Wolbachia–host interactions: connecting phenotype to genotype
... parasites of insects is becoming complicated as an increasing number of papers describe a richer picture of Wolbachia-mediated phenotypes in insects. The search for the molecular basis for this phenotypic variability has been greatly aided by the recent sequencing of several Wolbachia genomes. These ...
... parasites of insects is becoming complicated as an increasing number of papers describe a richer picture of Wolbachia-mediated phenotypes in insects. The search for the molecular basis for this phenotypic variability has been greatly aided by the recent sequencing of several Wolbachia genomes. These ...
History of Discoveries in Molecular Biology
... James Watson – American ornithologist Francis Crick – British Physicist ...
... James Watson – American ornithologist Francis Crick – British Physicist ...
Review A model for chromosome structure during the mitotic
... Figures 1^11 (overleaf). Diagrammatic representations of chromosome structure through the mitotic cell cycle. Drawings are not to scale. Telophase/G1 . (A) Longitudinal view of a segment of a decondensing chromosome during telophase or a decondensed chromosome during G1. The anaphase chromosome core ...
... Figures 1^11 (overleaf). Diagrammatic representations of chromosome structure through the mitotic cell cycle. Drawings are not to scale. Telophase/G1 . (A) Longitudinal view of a segment of a decondensing chromosome during telophase or a decondensed chromosome during G1. The anaphase chromosome core ...
The evolutionary history of human chromosome 7
... locally and then some copies were distributed to three locations on chromosome 7 by inversion events. This would imply that the inversions that occurred during hominoid evolution and the complex rearrangements that led to WBS in humans are mediated by the same duplicated sequences. Attempts to recon ...
... locally and then some copies were distributed to three locations on chromosome 7 by inversion events. This would imply that the inversions that occurred during hominoid evolution and the complex rearrangements that led to WBS in humans are mediated by the same duplicated sequences. Attempts to recon ...
Price, DK, Zhang, F, Ashley, CT and Warren, ST: The chicken FMR1 gene is highly conserved containing a CTT 5\' untranslated repeat and encodes an RNA-binding protein. Genomics 31:3-12 (1996).
... the mouse FMR1 cDNA Mc 2.17 (Ashley et al., 1993a) as a probe. Two positive clones were isolated, and the cDNA sequence was obtained. The first clone, Cc2-1, contained 900 bp of sequence homologous to the mouse and human FMR1 genes, including approximately 500 bp of coding sequence (Fig. 1). The oth ...
... the mouse FMR1 cDNA Mc 2.17 (Ashley et al., 1993a) as a probe. Two positive clones were isolated, and the cDNA sequence was obtained. The first clone, Cc2-1, contained 900 bp of sequence homologous to the mouse and human FMR1 genes, including approximately 500 bp of coding sequence (Fig. 1). The oth ...
Intellectual property rights and innovation: Evidence from
... From this empirical context, I construct three research designs to test for the effects of Celera’s IP on subsequent scientific research and product development outcomes. The first research design tests whether genes that ever had Celera’s IP differ in subsequent innovation, as of 2009, from genes ...
... From this empirical context, I construct three research designs to test for the effects of Celera’s IP on subsequent scientific research and product development outcomes. The first research design tests whether genes that ever had Celera’s IP differ in subsequent innovation, as of 2009, from genes ...