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The Nicotiana tabacum genome encodes two cytoplasmic
The Nicotiana tabacum genome encodes two cytoplasmic

... kit from Stratagene, according to the recommendations of the manufacturer. From the partial genomic phage library, 400 O00 plaques of the recombinant bacteriophage were plated at a density of about 50 O00 per plate, transferred onto Hybond N f membrane (Amersham) and screened with the thioredoxin X5 ...
Genetic diversity and epidemiology of infectious hematopoietic
Genetic diversity and epidemiology of infectious hematopoietic

gene mapping, marker gene mapping, marker-assisted
gene mapping, marker gene mapping, marker-assisted

... The aquaculture genetics projects were developed based on long-term efforts of selective breeding programs. These projects are dependent upon one another so that the maximum benefits will be obtained by using the integrated genetic improvement approaches. For instance, gene mapping will produce more ...
Gene Mapping, Marker-Assisted Selection, Gene Cloning, Genetic
Gene Mapping, Marker-Assisted Selection, Gene Cloning, Genetic

... The aquaculture genetics projects were developed based on long-term efforts of selective breeding programs. These projects are dependent upon one another so that the maximum benefits will be obtained by using the integrated genetic improvement approaches. For instance, gene mapping will produce more ...
Full-text PDF
Full-text PDF

... than one nucleic acid can be observed across the population [5]. These sites (i.e. loci) are called SNPs (single nucleotide polymorphisms). The sequence of pairs of DNA residues at each SNP site is called genotypes. The DNA residues which consist of genotypes are called alleles. There are two kinds ...
`Candidatus Phytoplasma mali`, `Candidatus Phytoplasma pyri` and
`Candidatus Phytoplasma mali`, `Candidatus Phytoplasma pyri` and

... similarity to that of any previously described ‘Candidatus Phytoplasma’ species’. In cases where phytoplasmas share ¢97?5 % 16S rDNA sequence similarity, description as different ‘Candidatus’ species is only recommended if there is an indication that these phytoplasmas clearly represent separate pop ...
Hailey Spelman - Determining Cellular Fate: Pre- and Postnatal Methylation Effects on Gene Expression
Hailey Spelman - Determining Cellular Fate: Pre- and Postnatal Methylation Effects on Gene Expression

... IV. Postnatal Methylation: Trends and Experimental Results Unlike prenatal methylation, trends seen in postnatal methylation support the possible use of demethylation to express a desired phenotype. As mentioned beforehand, intolerable hypermethylation can lead to oncogenesis. Extensive data has bee ...
CURRICULUM VITAE Name: Antonella Spinazzola Nationality
CURRICULUM VITAE Name: Antonella Spinazzola Nationality

... The study showed that MNGIE results from a deficiency of thymidine phosphorylase, an enzyme that catalyzes the reversible breakdown of thymidine to thymine plus deoxyribose-1-phosphate. The report underlies the importance of understanding the nature and sources of mitochondrial DNA precursor pools s ...
Amino acid and codon usage profiles: Adaptive changes in the
Amino acid and codon usage profiles: Adaptive changes in the

... single study as well; however, a significant overlap between the genes' cellular functions and their codon usage profiles has been noted in our results. Subsequently, we have shown that these patterns in codon usage changes are quite informative and might even be used to crudely predict functions. T ...
The relationship between higher‑order chromatin structure and
The relationship between higher‑order chromatin structure and

... sediments more slowly than bulk chromatin or a non‑expressed gene, whereas it sediments with bulk chromatin in non‑expressing cells [8,9,11]. However, can the β‑globin locus, whose regulation is known to be very complex in mammalian cells, be used as a paradigm for the other ∼25 000 genes in the gen ...
30. genetic code
30. genetic code

... three nitogenous (N) bases and the adjacent triplets overlap. This meant that at any particular point the same N-base occurs three times in a vertical manner instead of one which is expected on the basis of colinear model. This hypothesis, however, was not accepted on the following grounds : George ...
Imprinting capacity of gamete lineages in C. elegans
Imprinting capacity of gamete lineages in C. elegans

... with DNA methylation levels, with the maternally-derived alleles generally being more methylated than the paternally-derived alleles. Although reports of parent-of-origin effects in other organisms have been abundant, accounts of parent-of-origin phenomenon in C. elegans have been very rare. A scree ...
Chromatin DNA Methylayion
Chromatin DNA Methylayion

... Agents that inhibit DNA methyltransferase such as 5-aza-CdR stimulate tumor invasion and metastasis. ...
Visualizations of Microarray Data in Partek Genomics Suite 6.6
Visualizations of Microarray Data in Partek Genomics Suite 6.6

... PGS’s function Sort rows by prototype enables you to identify genes with similar expression profiles. For instance, if a gene with an interesting expression pattern has been detected, using Sort by prototype makes it possible to find other genes that have a similar pattern of intensity values. Altho ...
Structure and function of the GINS complex, a key component of the
Structure and function of the GINS complex, a key component of the

... Overproduction of PSF2, like PSF1, was also shown to be able to suppress to sld5-12. Further biochemical analysis showed that all four proteins were stoichiometric components of a 100 kDa complex present at constant levels through the cell cycle; the complex was named GINS. While Takayama et al. [5] ...
The Rad50 Signature Motif: Essential to ATP Binding and
The Rad50 Signature Motif: Essential to ATP Binding and

... The Mre11 protein contains a nuclease domain that is highly conserved and related to the lambda phosphatase family of phosphoesterases. The crystal structure of an Mre11 homolog from Pyrococcus furiosus shows five conserved motifs within the N-terminal half of Mre11 that coordinate two manganese ion ...
Algorithms for Finding Gene Clusters
Algorithms for Finding Gene Clusters

... associated genes of a gene cluster lie on the same DNA strand [20,12]. We take this into account by constructing signed permutations where the sign of a gene indicates the strand it lies on. We then determine all common intervals with the additional restriction that within each permutation, the elem ...
Taxonomic evaluation of the Streptomyces griseus clade using
Taxonomic evaluation of the Streptomyces griseus clade using

... of the genes also shows good resolution and robustness for differentiating most of the strains and is therefore of value for everyday use. MLSA is more suitable for discriminating strains that show .99 % 16S rRNA gene sequence similarity. DNA–DNA hybridization (DDH) between strains with representati ...
Conserved syntenic clusters of protein coding genes are missing in
Conserved syntenic clusters of protein coding genes are missing in

... compared to mammals [5,16]. Importantly, however, the evolution of avian genomes also appears to have involved a loss of protein coding genes, as the total number of unique identified avian coding genes (for example, 15,508 in chicken according to Ensembl release e71; [17,18]) is considerably smalle ...
Conserved syntenic clusters of protein coding genes are missing in birds
Conserved syntenic clusters of protein coding genes are missing in birds

... much shorter and fewer gaps and thus more complete than the version described in Hillier et al. [5]. Accordingly, this latest assembly contains the orthologs for many conserved genes that could not be found in the previous assembly (for example, [20]), and yielded significant BLAT-alignments for app ...
Curr. Microbiol. 42
Curr. Microbiol. 42

... The highly conserved block 5 in domain III is at the C-terminus of the activated toxin and is another major element that stabilizes the mature toxin [19, 27, 34]. Polymerase Chain Reaction (PCR) requires minute amounts of DNA and allows quick, simultaneous screening of many samples. This technique h ...
Online resources for genetic variation study-Part One
Online resources for genetic variation study-Part One

...  Allocated SNPs (dbSNP reference clusters chosen for genotyping) Adapted from Alanna Morrison, Human Genetics Center, Feb. 2007 lecture ...
Tracing the Thread of Plastid Diversity through the Tapestry of Life
Tracing the Thread of Plastid Diversity through the Tapestry of Life

... Indeed, although the relationships among the primary plastid lineages remain uncertain, several molecular phylogenetic analyses have placed the glaucocystophyte plastid as an outgroup to other primary plastids (Helmchen et al. 1995; Gray and Spencer 1996; Martin et al. 1998). If this is correct, and ...
Got Lactase? The Co-evolution of Genes and Culture
Got Lactase? The Co-evolution of Genes and Culture

International Journal of Antimicrobial Agents ksgA mutations confer
International Journal of Antimicrobial Agents ksgA mutations confer

... the growth of a wide variety of microorganisms, with reported low toxicity against plants, humans and other animals [2,4,5]. However, as an aminoglycoside, some degree of nephrotoxicity and ototoxicity is expected. Several Gram-negative bacteria, including Pseudomonas spp. and Escherichia coli strai ...
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Genomics

Genomics is a discipline in genetics that applies recombinant DNA, DNA sequencing methods, and bioinformatics to sequence, assemble, and analyze the function and structure of genomes (the complete set of DNA within a single cell of an organism). Advances in genomics have triggered a revolution in discovery-based research to understand even the most complex biological systems such as the brain. The field includes efforts to determine the entire DNA sequence of organisms and fine-scale genetic mapping. The field also includes studies of intragenomic phenomena such as heterosis, epistasis, pleiotropy and other interactions between loci and alleles within the genome. In contrast, the investigation of the roles and functions of single genes is a primary focus of molecular biology or genetics and is a common topic of modern medical and biological research. Research of single genes does not fall into the definition of genomics unless the aim of this genetic, pathway, and functional information analysis is to elucidate its effect on, place in, and response to the entire genome's networks.
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