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From essential to persistent genes: a functional
From essential to persistent genes: a functional

... quest for the ‘minimal genome’. However, ‘minimal sets’ of essential genes are strongly context-dependent and, in all prokaryotic genomes sequenced to date, not a single protein-coding gene is entirely conserved. Furthermore, a lack of consensus in the field as to what attributes make a gene truly e ...
Gene promoters dictate histone occupancy within genes
Gene promoters dictate histone occupancy within genes

... factor Spt6 in controlling nucleosome occupancy, particularly in transcribed regions. Previous work had shown that Spt6 contributed to nucleosome reassembly over coding regions of highly transcribed genes, and that effects on nucleosome occupancy were not closely correlated with effects on transcrip ...
Presentation - Cloudfront.net
Presentation - Cloudfront.net

... Clusters of Orthologous Groups of proteins (COGs) were delineated by comparing protein sequences encoded in complete genomes, representing major phylogenetic lineages. Each COG consists of individual proteins or groups of paralogs from at least 3 lineages and thus corresponds to an ancient conserve ...
The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI.
The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI.

... on chromosome XVI is rich in repetitive elements and contains three Ty elements, five additional LTRs and six tRNA genes. Such DNA duplications form large regions of partial gene synteny between these two chromosomes. An example is a section from nucleotide 834,000 to 860,000 on chromosome XVI and f ...
Vital Genes in the Heterochromatin of
Vital Genes in the Heterochromatin of

... Joint efforts are now required to complete the molecular characterization of the heterochromatic genome of D. melanogaster and to extend the work to other Drosophila species. This may allow us to characterize new heterochromatic genes and to learn how they have evolved. An important goal is also to ...
10709_2015_9875_MOESM4_ESM
10709_2015_9875_MOESM4_ESM

... at least one tag for a given gene being statistically significant, i.e., p-value < 0.05). Moreover, the authors manually curated the predicted datasets, filtering out genes considered by them to be false positives (e.g., genes with stronger fitness defect in the heterozygote (single allele knockout) ...
AthaMap web tools for database-assisted identification of
AthaMap web tools for database-assisted identification of

... behind the factor name (Table 1). The binding sites were taken directly from the published literature, which is regularly screened in the process of updating the TRANSFAC1 database with plant transcription factor data (2). The screens were performed on the most recent version of the A.thaliana genom ...
An Arabidopsis Mutant with a Reduced Leve1 of cabl40 RNA 1s a
An Arabidopsis Mutant with a Reduced Leve1 of cabl40 RNA 1s a

... DMA Gel Blot Analysis of the let Mutant a crossover between the marker and let. Analysis of F3 lines showed close linkage of let to the brevipedicellus (bp) marker on chromosome 4. The F3 progeny from 55 individual F2 bp lines from W8 (bp, eceriferum-2 [cer2], apetala-2 [ap2]) x let demonstrated tha ...
Genome browsers and other resources
Genome browsers and other resources

... 7. Plant databases - highlights from 10 papers  PLAZA 3.0: an access point for plant comparative genomics  PNRD: a plant non-coding RNA database  AraNet v2: an improved database of co-functional gene networks for the study of Arabidopsis thaliana and 27 other nonmodel plant species  Araport: the ...
Human housekeeping genes are compact
Human housekeeping genes are compact

... an approach has so far resulted in two lists of HK genes [3,4]. However, problems in probe design, measurement noise and other artifacts introduce inevitable errors in such lists. Because a northern blot experiment for each gene in each tissue is impractical, an independent test is needed to validat ...
pdf
pdf

... the promoter. But, as will be explored in the next chapter, this is not the case. RNA polymerase can bind to the lac promoter even when repressor is boudn to the lac operator. However, the polymerase cannot initiate transcription when juxtaposed to the repressor. ...
Boundary elements and nuclear organization
Boundary elements and nuclear organization

... may function as genomic insulators, are frequently found at the borders between bands and interbands of polytene chromosomes, suggestive of their role in this type of partitioning (M. Labrador and V. Corces, unpublished observations). The chicken b-globin 5′ HS4 insulator is present at the border be ...
GenomeBrowser - CBS
GenomeBrowser - CBS

... UCSC genome browser Basic functionalities • Finding a gene • by name • by sequence • Gene structure • Sequence orthologues • Single Nucleotide Polymorphisms • Gene Sorter - sort according to expression, homology ... • Custom tracks ...
pdf
pdf

... CFI and CFII are cleavage factors. PAP is the polyA polymerase. CFI, CFII and PAP form a complex that binds to the nascent RNA at the cleavage site, directed by the CPSF specificity factor. CstF is an additional protein implicated in this process in vitro, but its precise function is currently unkno ...
Uncovering evolutionary patterns of gene expression using
Uncovering evolutionary patterns of gene expression using

... different genes, which might represent a considerable fraction of the transcriptome. This has been well documented in experiments of artificial selection on odor-guided behaviour and mating speed in D. melanogaster [15,16]. Tempo and mode of transcriptome evolution The logical extension from intrasp ...
blumberg-lab.bio.uci.edu
blumberg-lab.bio.uci.edu

... ‘RED’ Chromatin ● Is replicated first and has most origins of replication ● Concentrates nucleosome remodeling ● Contains key protein in chromosome structure ● Genes are linked to behavior and tissue specific processes ...
1st set of Journal Clubs this Wednesday!
1st set of Journal Clubs this Wednesday!

... Monod showed that Z+Y- mutants did not take up a radioactively labeled galactoside in the presence of inducer. He concluded that lacY must encode an enzyme responsible for transporting the galactoside into cells: called it galactoside permease. ...
Identification of a Class of Chromatin Boundary Elements
Identification of a Class of Chromatin Boundary Elements

... after five cycles of the enrichment protocol (see below) and selected by alpha complementation. From 101 plasmids, 11 cBEs were obtained, and of these, 4 had sequences related to cBE28. Both strands of the cBEs were dideoxy sequenced, and sequence comparisons and database searches were performed wit ...
CHAPTER  1 LITERATURE  SURVEY
CHAPTER 1 LITERATURE SURVEY

... latter case, this reverse genetics system was utilised in a fascinating study to systematically alter the phenotype of the virus by manipulation of the genome (Wertz et al. 1998; Ball et al. 1999). Relative levels of gene expression in VSV, as in other members of the order Mononegavirales, is contro ...
Posttranscriptional Control of Chloroplast Gene Expression
Posttranscriptional Control of Chloroplast Gene Expression

... resection to a stem-loop at the 3⬘ end. These processing steps are catalyzed by a chloroplast degradosome similar to that of Escherichia coli, consisting of several nucleus-encoded RNA-binding proteins, including exo- and endonucleases (9). As in bacteria, polyadenylation appears to play an importan ...
Caenorhabditis elegans chromosome arms are anchored to the
Caenorhabditis elegans chromosome arms are anchored to the

... occur in eukaryotic cells. B-type lamin and emerin were found to be associated with large domains up to several megabases in length, which cover about 40% of the genome in mouse and human cells [6,7]. In flies, however, the size and the coverage of lamin-associated regions were not determined precis ...
The tryptophan biosynthetic pathway
The tryptophan biosynthetic pathway

... acids encoded by the sequence. An essential feature of this attenuation mechanism is the synchronization of translation of a 14-residue leader peptide coding region, trpL, with transcription of the operon’s leader region. Synchronization is achieved by exploiting features of the initial segment of t ...
The spatial organization of human chromosomes within the nuclei of
The spatial organization of human chromosomes within the nuclei of

... periphery (shell 1 > shell 5, P ≤ 0.03) (Fig. 4). This suggests that the organization of chromosomes within human fibroblasts is broadly similar to that in lymphoblasts. However, there were also some notable differences between these two cell types in the way in which signals from some chromosomes w ...
I = -[1/3*log 2 (1/3)+ 1/3*log 2 (1/3)+ 1/3*log 2 (1/3)] + 4.32 = 2.73
I = -[1/3*log 2 (1/3)+ 1/3*log 2 (1/3)+ 1/3*log 2 (1/3)] + 4.32 = 2.73

... • Very fast searches (MySQL database) • Handle introns in RNA/DNA alignments • Data for more that 30 genomes (human, mouse, rat…) ...
A Novel Chimeric Low-Molecular-Weight Glutenin
A Novel Chimeric Low-Molecular-Weight Glutenin

... Phylogenetic analysis among LMW-GS and other storage protein genes: The 27 gene sequences coding for LMW-GS, gliadins, and hordeins from GenBank were used to construct a homology tree, including 3 LMW-s-type, 12 LMW-m-type, and 5 LMW-i-type glutenin genes as well as 4 gliadins and 3 hordein genes fr ...
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Short interspersed nuclear elements (SINEs)

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