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GeNeViSTA Coffin Siris Syndrome: A Disorder of SWI/SNF Pathway
GeNeViSTA Coffin Siris Syndrome: A Disorder of SWI/SNF Pathway

... have less coarse craniofacial appearances and behavioral abnormalities. SMARCE1 mutations have a wide spectrum of manifestations from moderate to severe intellectual disability. Patients with ARID1A mutations have a wide spectrum of manifestations from mild to severe intellectual disability and seri ...
Multiple Trypanosoma cruzi antigens containing tandemly repeated
Multiple Trypanosoma cruzi antigens containing tandemly repeated

... Nine clones encoding T. cruzi antigens have been characterized in the present study. Some of the fusion proteins, expressed from these clones, reacted with a large proportion of Chagasic sera, collected in distant geographical regions, thus showing that they represent parasite antigens which are con ...
Sequence Alignment
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... •Bottom sequence has more identities and similarities overall, but lots of little gaps. The matches near the C-terminal are absurd. •Look at the short segment after the first gap in the lower sequence: gained 3 identities ...
Drosophila windpipe codes for a leucine
Drosophila windpipe codes for a leucine

... protein has 677 residues with a potential signal peptide cleavage sequence located between amino acids 20 and 21 (ANA-TP). Based on hydropathy analysis, residues 451– 472 form a transmembrane domain, followed by an acidic-rich ‘stop-transfer’ sequence (KRKC). The predicted extracellular domain conta ...
The Two Faces of Higher Eukaryotic DNA Replication Origins
The Two Faces of Higher Eukaryotic DNA Replication Origins

... the model is that the shortest nascent leading strands appear to become progressively longer at increasing distances from the origin of bidirectional replication (Vassilev et al., 1990). However, this apparent conflict may not be serious because the nascent strand size measurements were performed ov ...
When epigenetics meets alternative splicing: the roles of DNA
When epigenetics meets alternative splicing: the roles of DNA

... confronts ‘bumpers’ scattered along both exons and introns. Remarkably, all minigenes generated from genes of high GC content fail to splice properly when transfected into mammalian cells [7]. What then helps the splicing machinery to recognize the exons in these ‘evolutionarily new’ regions? It is ...
The Rh Antigen D: A Review for Clinicians
The Rh Antigen D: A Review for Clinicians

... with normal D expression (D+), RBCs with a partial weak D antigen are agglutinated by reactive monoclonal anti-D more weakly or variably (i.e., some anti-D react strongly and some react weakly). This variable reactivity of anti-D with RBCs expressing a partial D antigen can lead to confusion (see be ...
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Molecular targets of oxidative stress

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Differential expression of surface membrane Trypanosoma congolense
Differential expression of surface membrane Trypanosoma congolense

... antigens on bovine monocytes activated with recombinant cytokines and during Trypanosoma congolense infection . Onderstepoort Journal of Veterinary Research, 67:289-296 The expression of surface membrane antigens on peripheral blood monocytes (PBM) of cattle of the Boran and N'Dama breeds activated ...
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Staphylococcus aureus infection induces protein A–mediated

... idiotype B cells. The effects of SpA during natural infection, however, have not been addressed. Acutely activated B cells, or plasmablasts (PBs), were analyzed to dissect the ongoing immune response to infection through the production of monoclonal antibodies (mAbs). The B cells that were activated ...
Epigenetics in mood disorders
Epigenetics in mood disorders

... including enhanced glucocorticoid negative feedback sensitivity due to an increase in GR in the offspring of high LG mothers. Glucocorticoid receptor expression is regulated at the level of RNA, through splice variation in the 50 untranslated region (UTR) of exon 1 [31]. Alterations in maternal beha ...
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Antigen processing and presentation

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... which translocates peptides from the cytoplasm into the ER. Prior to entering the ER, peptides are derived from the degradation of proteins, which can be of viral- or self origin. Degradation of proteins is mediated by cytosolic- and nuclear proteasomes, and the resulting peptides are translocated i ...
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Recombinant Materials Form

... 31. Will experiments involve work with the following influenza viruses? i.Human H2N2 (1957-1968)-virus containing H2 hemagglutinin (HA) gene in cold-adapted, live attenuated vaccine strains (e.g., A/Ann Arbor/6/60 H2N2) in which the segments with mutations conferring temperature sensitivity and att ...
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... derived from seaweed • It dissolves in boiling water and then gels as it cools ...
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... believed that plants obtain everything they need from the soil. Current understanding does not support this belief. Which of the following would least likely be absorbed from the soil? F Minerals G H2O H Nitrates J CO2 ...
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... merozoites are produced. During this period there are no clinical symptoms of malaria. The released merozoites invade erythrocytes within about 30 seconds to commence erythrocytic schizogony consisting of four stages: merozoites develop into the ring stage (immature trophozoites visible in periphera ...
The production of pharmaceutical proteins from the milk of
The production of pharmaceutical proteins from the milk of

... Many experiments have led to the conclusion that native genes are often efficiently expressed as transgenes whereas their cDNA counterparts are not. The presence of intron, which is generally not required in cultured cells, is thus of paramount importance for transgenes (Brinster et al, 1988; Palmit ...
PPT - Altogen Biosystems
PPT - Altogen Biosystems

... 3. Prepare transfection complexes by mixing 40 µl of serum-free medium, 5.5 µl of transfection reagent, and • 750 ng DNA (or mRNA), or • 30 nM - 50 nM of siRNA (or microRNA) *Referred to a final volume including growth medium 4. Incubate transfection complexes at RT for 15 - 30 minutes 5. Optional: ...
Immuun nr 3 2015 - Dutch Society for Immunology
Immuun nr 3 2015 - Dutch Society for Immunology

... Richard Jaspers PhD of the VU University Amsterdam: “Human Movement Sciences teach cardiology, orthopaedia and exercise physiology. Maastricht also focuses on nutrition and Nijmegen on lung diseases. Immunology has until now remained outside the scope. And if there is some knowledge, this only regar ...
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Hyalophora cecropia Innate Immunity in Insects, Function and Regulation of Hemolin from

... the mammalian gut epithelium is in constant contact with bacteria and bacterial products. Although these factors are normally pro-inflammatory for other cell types, they do not induce an immune response by the gut epithelial cells. However, the epithelial cells do respond to pathogenic bacteria. In ...
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Course Objectives / Outline MLAB 1235 Immunology/Serology 1

... Describe the function of the secondary lymphoid organs: spleen and lymph nodes. ...
Magnusiomyces capitatus (de Hoog et al.) de Hoog et Smith
Magnusiomyces capitatus (de Hoog et al.) de Hoog et Smith

... The viability of ATCC® products is warranted for 30 days from the date of shipment, and is valid only if the product is stored and cultured according to the information included on this product information sheet. ATCC lists the media formulation that has been found to be effective for this strain. W ...
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DNA vaccination



DNA vaccination is a technique for protecting an animal against disease by injecting it with genetically engineered DNA so cells directly produce an antigen, resulting in a protective immunological response. Several DNA vaccines have been released for veterinary use, and there has been promising research using the vaccines for viral, bacterial and parasitic diseases, as well as to several tumour types. Although only one DNA vaccine has been approved for human use, DNA vaccines may have a number of potential advantages over conventional vaccines, including the ability to induce a wider range of immune response types.
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