Spontaneous mutagenesis in exponentially growing and sta
... 1993; Foster, 1999; Bull et al., 2000). It has been found that mutations in the dnaE gene encoding the a polymerizing subunit of Escherichia coli DNA polymerase III (Pol III), the enzyme responsible for duplication of the bacterial chromosome, lead to changes in spontaneous mutagenesis in replicatin ...
... 1993; Foster, 1999; Bull et al., 2000). It has been found that mutations in the dnaE gene encoding the a polymerizing subunit of Escherichia coli DNA polymerase III (Pol III), the enzyme responsible for duplication of the bacterial chromosome, lead to changes in spontaneous mutagenesis in replicatin ...
The Immune System
... that circulate freely in the blood plasma. When they encounter a bacterial or fungal cell wall, these proteins aggregate to form a membrane attack complex that inserts itself into the foreign cell’s plasma membrane, forming a pore like that produced by natural killer cells (figure 57.5). Water enter ...
... that circulate freely in the blood plasma. When they encounter a bacterial or fungal cell wall, these proteins aggregate to form a membrane attack complex that inserts itself into the foreign cell’s plasma membrane, forming a pore like that produced by natural killer cells (figure 57.5). Water enter ...
Recognition of viruses in the cytoplasm by RLRs and other
... RNA products reverse-transcribed from the genome of DNA viruses through cytoplasmic RNA polymerase III (14, 15). By using chemically synthesized polydA:dT, these studies proposed this novel role of RIG-I as an ‘indirect’ DNA sensor. In addition, the ‘panhandle’ structure found in the influenza genom ...
... RNA products reverse-transcribed from the genome of DNA viruses through cytoplasmic RNA polymerase III (14, 15). By using chemically synthesized polydA:dT, these studies proposed this novel role of RIG-I as an ‘indirect’ DNA sensor. In addition, the ‘panhandle’ structure found in the influenza genom ...
Identification of Immunogenic Human Melanoma Antigens in a
... may increase resistance to melanoma. The most convincing evidence that this concept is correct is that immunization to melanoma vaccines can prevent this cancer in syngeneic mice (2—4).The protection conferred is specific (2), i.e., mice immu nized with control vaccine are not protected against me ...
... may increase resistance to melanoma. The most convincing evidence that this concept is correct is that immunization to melanoma vaccines can prevent this cancer in syngeneic mice (2—4).The protection conferred is specific (2), i.e., mice immu nized with control vaccine are not protected against me ...
Immune response to fungal infections
... The immune mechanisms of defence against fungal infections are numerous, and range from protective mechanisms that were present early in evolution (innate immunity) to sophisticated adaptive mechanisms that are induced specifically during infection and disease (adaptive immunity). The first-line inn ...
... The immune mechanisms of defence against fungal infections are numerous, and range from protective mechanisms that were present early in evolution (innate immunity) to sophisticated adaptive mechanisms that are induced specifically during infection and disease (adaptive immunity). The first-line inn ...
Trawling DNA Databases For Partial Matches: What Is The FBI
... trawls have led police to serial rapists and murderers who have long eluded detection.4 Indeed, even dead men have been “accused” through this technology.5 In addition, database trawls have considerable potential to solve common property crimes.6 In one case, an observant police inspector in Finland ...
... trawls have led police to serial rapists and murderers who have long eluded detection.4 Indeed, even dead men have been “accused” through this technology.5 In addition, database trawls have considerable potential to solve common property crimes.6 In one case, an observant police inspector in Finland ...
Accepted version - Queen Mary University of London
... clones were amplified and sequenced. Missense mutation(s) were identified in each case. In clones 1, 2, 4 and 5, there was C/T transition at position 374, compared with the TcNTR gene amplified from sensitive clones. In the protein, this would result in replacement of the evolutionarily conserved Pr ...
... clones were amplified and sequenced. Missense mutation(s) were identified in each case. In clones 1, 2, 4 and 5, there was C/T transition at position 374, compared with the TcNTR gene amplified from sensitive clones. In the protein, this would result in replacement of the evolutionarily conserved Pr ...
Understanding Immune REcognition
... MHC molecule. This is because the molecular structure of the MHCAntigen complex is arranged so that some of the polymorphic amino acids of the MHC molecule are in direct contact with the TCR – Therefore T cell recognition of antigen is said to be MHC ‘restricted’. ...
... MHC molecule. This is because the molecular structure of the MHCAntigen complex is arranged so that some of the polymorphic amino acids of the MHC molecule are in direct contact with the TCR – Therefore T cell recognition of antigen is said to be MHC ‘restricted’. ...
Tetanus Toxoid Pulsed Monocyte Vaccination For Augmentation Of
... Our group has been intrigued by the idea that increasing local inflammation may represent the best stimulus for arteriogenesis. We have exploited the homing of Mo to areas of collateral growth for cell transplantation studies.21 Intriguingly, transplantation of allogeneic Mo resulted in the strongest ...
... Our group has been intrigued by the idea that increasing local inflammation may represent the best stimulus for arteriogenesis. We have exploited the homing of Mo to areas of collateral growth for cell transplantation studies.21 Intriguingly, transplantation of allogeneic Mo resulted in the strongest ...
Chapter 13 Mutation, DNA Repair, and Recombination
... Mismatch Repair in E. coli Mismatching or mispairing of G and T (DNA polymerase/exonuclease proofreading activity) The A in GATC sequences is methylated subsequent to DNA replication. In newly replicated DNA, the parental strand is methylated, but the new strand is not. This difference allows ...
... Mismatch Repair in E. coli Mismatching or mispairing of G and T (DNA polymerase/exonuclease proofreading activity) The A in GATC sequences is methylated subsequent to DNA replication. In newly replicated DNA, the parental strand is methylated, but the new strand is not. This difference allows ...
Negative Individuals
... In this report, we determined two individuals to be Le(-) by RBC and immunostaining of gastric mucosa and isolated mRNA from gastric mucosa of these two individuals. Those COS cells transfected with Le(-) cDNA did not express Le antigens, suggesting the presence of mutation($ in Le(-) cDNA making Le ...
... In this report, we determined two individuals to be Le(-) by RBC and immunostaining of gastric mucosa and isolated mRNA from gastric mucosa of these two individuals. Those COS cells transfected with Le(-) cDNA did not express Le antigens, suggesting the presence of mutation($ in Le(-) cDNA making Le ...
Diversity of DNA methyltransferases that recognize asymmetric
... Multiple lines of evidence suggest that some DNA MTases function as dimers. Further, functional analysis of many restriction-modification systems showed the presence of more than one or fused MTase genes. It was proposed that presence of two MTases responsible for the recognition and methylation of ...
... Multiple lines of evidence suggest that some DNA MTases function as dimers. Further, functional analysis of many restriction-modification systems showed the presence of more than one or fused MTase genes. It was proposed that presence of two MTases responsible for the recognition and methylation of ...
Non-Mendelian Inheritance
... mutant me1 in which cpDNAs were constitutively methylated to high levels in both mt+ and mt– vegetative cells. Sager and Grabowy countered this conclusion with their observation that additional methylation occurred during gametogenesis of mt+, but not mt– cells carrying the me1 mutation. And additio ...
... mutant me1 in which cpDNAs were constitutively methylated to high levels in both mt+ and mt– vegetative cells. Sager and Grabowy countered this conclusion with their observation that additional methylation occurred during gametogenesis of mt+, but not mt– cells carrying the me1 mutation. And additio ...
The clinical implications of antitumor immunity in head and neck
... only certain immune cells express MHC class II molecules. Macrophages and DCs express both MHC class I and II molecules and as such are termed professional antigen presenting cells (APCs). The APC function of M/s and DCs may be compartmentalized, as DCs are present in the mucosa of the upper aerodig ...
... only certain immune cells express MHC class II molecules. Macrophages and DCs express both MHC class I and II molecules and as such are termed professional antigen presenting cells (APCs). The APC function of M/s and DCs may be compartmentalized, as DCs are present in the mucosa of the upper aerodig ...
Does Activation of the TALl Gene Occur in a Majority
... locus. Thus, TALl rearrangement, whether mediated by chromosome translocation or by talld recombination, is the most common genetic defect known to be associated with T-ALL.’“I7 The presentation features and clinical outcomes of T-ALL patients who have TALl gene rearrangements are almost indistingui ...
... locus. Thus, TALl rearrangement, whether mediated by chromosome translocation or by talld recombination, is the most common genetic defect known to be associated with T-ALL.’“I7 The presentation features and clinical outcomes of T-ALL patients who have TALl gene rearrangements are almost indistingui ...
Hammond 1 Regulation of gene expression during flocculation in
... plasmids, showing two distinct bands (one corresponding to the vector and one to the insert) were sent to sequencing for confirmation (Molecular Biology Core Facility, UTK). The plasmids for which the DNA sequence was confirmed were then digested with XhoI and EcoRI and analyzed on agarose gel elect ...
... plasmids, showing two distinct bands (one corresponding to the vector and one to the insert) were sent to sequencing for confirmation (Molecular Biology Core Facility, UTK). The plasmids for which the DNA sequence was confirmed were then digested with XhoI and EcoRI and analyzed on agarose gel elect ...
GROW`N`GLOW: THE ACE1 TWO-HYBRID
... bioluminescent cnidarian Aequorea victoria4 and the metallothionein encoding CUP1 gene5. ...
... bioluminescent cnidarian Aequorea victoria4 and the metallothionein encoding CUP1 gene5. ...
Comprehensive analyses of tumor immunity: implications for cancer
... Results: We analyze tumor-infiltrating immune cells in over 10,000 RNA-seq samples across 23 cancer types from The Cancer Genome Atlas (TCGA). Our computationally inferred immune infiltrates associate much more strongly with patient clinical features, viral infection status, and cancer genetic alter ...
... Results: We analyze tumor-infiltrating immune cells in over 10,000 RNA-seq samples across 23 cancer types from The Cancer Genome Atlas (TCGA). Our computationally inferred immune infiltrates associate much more strongly with patient clinical features, viral infection status, and cancer genetic alter ...
May 2006 - InvivoGen
... antibody is another way to improve its efficacy, allowing higher circulating levels, less frequent administration and reduced doses. This can be achieved by enhancing the binding of the Fc region to neonatal FcR (FcRn). FcRn, which is expressed on the surface of endothelial cells, binds the IgG in a ...
... antibody is another way to improve its efficacy, allowing higher circulating levels, less frequent administration and reduced doses. This can be achieved by enhancing the binding of the Fc region to neonatal FcR (FcRn). FcRn, which is expressed on the surface of endothelial cells, binds the IgG in a ...
Antiviral activity of Engystol® and Gripp-Heel®: an in
... virus, rhinovirus and coxsackievirus [6,7]. Other in vitro studies have demonstrated that Engystol® exerts modulatory effects on the immune system in terms of phagocytic activity, granulocyte function and improved humoral response [8-13]. However, the research conducted on this topic so far falls sh ...
... virus, rhinovirus and coxsackievirus [6,7]. Other in vitro studies have demonstrated that Engystol® exerts modulatory effects on the immune system in terms of phagocytic activity, granulocyte function and improved humoral response [8-13]. However, the research conducted on this topic so far falls sh ...
Candida albicans Daniel Gozalbo , Victoria Maneu , María Luisa Gil
... Candida species are the most frequent cause of mucosal and invasive fungal infections (1-3). The leading cause of candidiasis, Candida albicans, resides as a commensal of the human mucosae and the gastrointestinal tract. In immunocompromised hosts, saprophytic colonization often leads to opportunist ...
... Candida species are the most frequent cause of mucosal and invasive fungal infections (1-3). The leading cause of candidiasis, Candida albicans, resides as a commensal of the human mucosae and the gastrointestinal tract. In immunocompromised hosts, saprophytic colonization often leads to opportunist ...
Th17 responses to Mycobacterium tuberculosis are compromised in
... infected TLR2-/- animals, 54 days post-infection. In line with the histological analysis, at late ...
... infected TLR2-/- animals, 54 days post-infection. In line with the histological analysis, at late ...
DNA vaccination
DNA vaccination is a technique for protecting an animal against disease by injecting it with genetically engineered DNA so cells directly produce an antigen, resulting in a protective immunological response. Several DNA vaccines have been released for veterinary use, and there has been promising research using the vaccines for viral, bacterial and parasitic diseases, as well as to several tumour types. Although only one DNA vaccine has been approved for human use, DNA vaccines may have a number of potential advantages over conventional vaccines, including the ability to induce a wider range of immune response types.