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The ATM repair pathway inhibits RNA polymerase I transcription in
The ATM repair pathway inhibits RNA polymerase I transcription in

... 2. Gene expression takes time: Typically more than an hour from DNA to protein. Most rapidly 15 minutes. ...
XistAR write up
XistAR write up

... cells analyzed were from TS (trophoblast stem) and XEN (extra-embryonic endoderm) which both exhibit iXCI. To examine the involvement of XistAR in random inactivation, they examined antisense expression in EpiSC (epiblast stem cells). Since it is impossible to deduce allele-specific expression in ra ...
Microbial Genetics Chromosomes Genes Related to Obesity in the
Microbial Genetics Chromosomes Genes Related to Obesity in the

... function with 100,000 genes  and now the number has  dropped to ~35,000 genes  although this is still a hot  topic in research ...
101 -- 2006
101 -- 2006

... a) It has high energy phosphate bonds. c) Its hydrolysis is endergonic. b) Its synthesis is exergonic. d) It is extremely stable. e) It is readily obtained from an organism's environment. __ 39. When NAD+ is converted to NADH, it was a) oxidized b) reduced c) phosphorylated d) denatured e) hydrolyze ...
PowerPoint 簡報
PowerPoint 簡報

... • Some promoters require activators to stimulate the transition from closed to open complex. • Activators that stimulate this kind of promoter work by triggering a conformation change in either RNA polymerase or DNA. • This mechanism is an example of allostery. • One activator, NtrC, interacts with ...
Unit C Outline
Unit C Outline

MicroRNAs: key participants in gene regulatory networks
MicroRNAs: key participants in gene regulatory networks

... translational repression can be easily deleted just by releasing the miRNAs, rather than destruction and synthesis of new mRNAs. The other miRNAs were thought to interact with target mRNAs by limited complementary and suppressed translation as well [4–6]. Many studies have shown, however, that the a ...
No Slide Title
No Slide Title

... The forward rate constant for RNA Pol binding to promoters is faster than random diffusion (that limits the constant to 108/M-1Sec-1). The measured rate constant for association with a 60 bp target is 1014/M-1Sec-1. If the target is the whole genome the rate constant is around 1014/M-1Sec-1. But how ...
CHAPTER 17
CHAPTER 17

... each gene to control its own regulation. FIGURE 17.18 Concept check: A pre-mRNA is recognized by just one splicing repressor that binds to the 3’-end of the third intron. The third intron is located between exon 3 and exon 4. After splicing is complete, would you expect the mRNA to contain exon 3 an ...
Formation of Amino Acids
Formation of Amino Acids

... Metabolism is the process of a cell carrying out life functions. Growth, respiration, movement, and everything else. For a long time, scientists didn’t know how the cell knew when to do each of these kinds of things. We now know it comes down to two things: - Proteins - DNA ...
Protocol S1.
Protocol S1.

... prediction algorithms used were SignalP (HMM/Smean score method) [10] for signal peptides and TMHMM (First60 score cutoff greater than 10) [11] for transmembrane domains. In order to calculate the fraction of proteins within a category that contained a given motif, the overlap between that category ...
DNA Replication - Gadjah Mada University
DNA Replication - Gadjah Mada University

... understood, but it has been shown to involve an array of controlling signals. a. Jacob and Monod (1961) proposed the operon model to explain prokaryotic gene regulation, showing that a genetic switch is used to control production of the enzymes needed to metabolize lactose. Similar systems control m ...
Chapter 14 Overview: The Flow of Genetic Information
Chapter 14 Overview: The Flow of Genetic Information

... During translation, the sequence of codons along an mRNA molecule is translated into a sequence of amino  acids making up the polypeptide chain.  During translation, the codons are read in the 5’ 3’ direction along the mRNA.  Each codon specifies which one of the 20 amino acids will be incorporated ...
RNA polymerase
RNA polymerase

... turn genes OFF example if bacterium has enough tryptophan then it STOP doesn’t need to make enzymes used to build tryptophan  turn genes ON example if bacterium encounters new sugar (energy GO source), like lactose, then it needs to start making enzymes used to digest lactose ...
Protein Synthesis
Protein Synthesis

... What are the 3 types of RNA? A sequence of 3 nucleotides on the mRNA strand that codes for a specific amino acid is called a what? What is the name of the bond that is formed between two amino acids? How do amino acids get into the body in the ...
CH. 12.3 : DNA, RNA, and Protein
CH. 12.3 : DNA, RNA, and Protein

pGLO Plasmid Map
pGLO Plasmid Map

... unneeded proteins, which would put the organism at a competitive disadvantage. The sugar arabinose is both a source of energy and a source of carbon. E. coli bacteria produce three enzymes (proteins) needed to digest arabinose as a food source. The genes that code for these enzymes are not expressed ...
video slide - Wild about Bio
video slide - Wild about Bio

... transcription is immediately translated without more processing In a eukaryotic cell, the nuclear envelope separates transcription ...
Gene Expression and DNA Replication
Gene Expression and DNA Replication

... • Most DNA occurs in nature as a right-handed doublehelical molecule known as Watson-Crick DNA or BDNA. • The hydrophilic sugar-phosphate backbone of each strand is on the outside of the double helix. The hydrogen-bonded base pairs are stacked in the center of the molecule. • There are about 10 ba ...
Supplementary Information (doc 884K)
Supplementary Information (doc 884K)

... A set of pre-miRNAs, composed by four different double-stranded oligonucleotides for each gene, targeting the expression of D2 or Gpr88 (BLOCK-IT™ miR RNAi Select) were acquired from Invitrogen. Recombinant expression clones carrying the DNA sequence for stable RNA interference were produced using t ...
Efficient delivery of small interfering RNA to plant cells
Efficient delivery of small interfering RNA to plant cells

... Available online 15 May 2006 ...
B. Eukaryotic RNA polymerases
B. Eukaryotic RNA polymerases

... a) Nucleoli are sites of ribosomal RNA (rRNA production) synthesis and the beginning of ribosome assembly 2. Nucleolar organizing regions a) Area where many copies of rRNA genes (except 5S rRNA) are being actively transcribed 3. Ribosomal RNA is not translated VII.EUKARYOTIC ...
AP Review
AP Review

... The role of DNA in heredity was first studied by using bacteria and viruses. Griffith (1928) was studying streptococcus pneumonia. Transformation: a change in genotype and phenotype due to the assimilation of external DNA by a cell. Avery (1944) purified various chemicals from the heat killed bacter ...
Protein Synthesis: Transcription and Translation
Protein Synthesis: Transcription and Translation

... Messenger RNA contains genetic information. It is a copy of a portion of the DNA. It carries genetic information from the gene (DNA) out of the nucleus, into the cytoplasm of the cell where it is translated to produce protein. Ribosomal RNA (rRNA) This type of RNA is a structural component of the ri ...
video slide - CARNES AP BIO
video slide - CARNES AP BIO

... • These triplets are the smallest units of uniform length that can code for all the amino acids • Example: AGT at a particular position on a DNA strand results in the placement of the amino acid serine at the corresponding position of the polypeptide to be produced Copyright © 2008 Pearson Education ...
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RNA silencing

RNA silencing (associated with the concept of post-transcriptional gene silencing or RNA interference) refers to a family of gene silencing effects by which the expression of one or more genes is downregulated or entirely suppressed by non-coding RNAs, particularly small RNAs. It may also refer to the introduction of a synthetic antisense RNA molecule used in scientific experiments on gene expression. RNA silencing may also be defined as sequence-specific regulation of gene expression triggered by double-stranded RNA (dsRNA). RNA silencing mechanisms are highly conserved in most eukaryotes. The most common and well-studied example is RNA interference (RNAi), in which endogenously expressed microRNA (miRNA) or exogenously derived small interfering RNA (siRNA) induces the degradation of complementary messenger RNA. Other classes of small RNA have been identified, including piwi-interacting RNA (piRNA) and its subspecies repeat associated small interfering RNA (rasiRNA).
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