The hepatitis C virus Core protein is a potent nucleic acid chaperone
... Core protein. Here we report that Core protein chaperones the annealing of complementary DNA and RNA sequences and the formation of the most stable duplex by strand exchange. These results show that the HCV Core is a nucleic acid chaperone similar to retroviral NC proteins. We also ®nd that the Core ...
... Core protein. Here we report that Core protein chaperones the annealing of complementary DNA and RNA sequences and the formation of the most stable duplex by strand exchange. These results show that the HCV Core is a nucleic acid chaperone similar to retroviral NC proteins. We also ®nd that the Core ...
Analysis of the 3′-terminal nucleotide sequence of vesicular
... slice in 1 ml of 100 m M N a C l , 10 mM Tris-HCl, 1 mM EDTA, 0.1% sodium dodecyl sulphate overnight. The solution was then filtered through a 50-ul DEAE-cellulose column. After washing with water, the oligonucleotide was eluted with 1 M triethylamine carbonate pH 10 and recovered by several cycles ...
... slice in 1 ml of 100 m M N a C l , 10 mM Tris-HCl, 1 mM EDTA, 0.1% sodium dodecyl sulphate overnight. The solution was then filtered through a 50-ul DEAE-cellulose column. After washing with water, the oligonucleotide was eluted with 1 M triethylamine carbonate pH 10 and recovered by several cycles ...
biological background the central dogma of molecular biology
... • Consist of very long, linear double stranded DNA. • The DNA is complexed with twice as much protein (histones organized in nucleosomes). • The protein helps compact it into the nucleus. ...
... • Consist of very long, linear double stranded DNA. • The DNA is complexed with twice as much protein (histones organized in nucleosomes). • The protein helps compact it into the nucleus. ...
Chpt10_TxnRNAPol.doc
... f. The reaction progresses (the enzyme moves) about 50 nts per sec. This is much slower than the rate of replication (about 1000 nts per sec). g. If the template is topologically constrained, the DNA ahead of the RNA polymerase becomes overwound (positive superhelical turns) and the DNA behind the R ...
... f. The reaction progresses (the enzyme moves) about 50 nts per sec. This is much slower than the rate of replication (about 1000 nts per sec). g. If the template is topologically constrained, the DNA ahead of the RNA polymerase becomes overwound (positive superhelical turns) and the DNA behind the R ...
What do we need DNA for?
... tube can prevent false priming in the initial round of DNA replication) Annealing: The default is usually 55°C. This temperature variable is the most critical one for getting a successful PCR reaction. This is the best variable to start with when trying to optimize a PCR reaction for a specific set ...
... tube can prevent false priming in the initial round of DNA replication) Annealing: The default is usually 55°C. This temperature variable is the most critical one for getting a successful PCR reaction. This is the best variable to start with when trying to optimize a PCR reaction for a specific set ...
DNA and RNA
... twist the staircase to remove the spiral so we are left with the ladder-like structure depicted in Figure 4.1. The two backbones to this ladder are composed of sugars (S in the figure) and phosphates (P); they need not concern us further. The whole action of DNA is in the rungs. Each rung of the lad ...
... twist the staircase to remove the spiral so we are left with the ladder-like structure depicted in Figure 4.1. The two backbones to this ladder are composed of sugars (S in the figure) and phosphates (P); they need not concern us further. The whole action of DNA is in the rungs. Each rung of the lad ...
Normalization of gene expression measurements in tumor tissues
... RNA amount in some studies.3,9 This does not prove that these genes are invariably expressed during tumorigenesis. In addition, total RNA quantification does not correct for RNA quality differences (eg due to storage), nor for differences in reverse transcriptase efficiencies between samples.27 It i ...
... RNA amount in some studies.3,9 This does not prove that these genes are invariably expressed during tumorigenesis. In addition, total RNA quantification does not correct for RNA quality differences (eg due to storage), nor for differences in reverse transcriptase efficiencies between samples.27 It i ...
Structural Studies on the Dosage Compensation Complex from
... residues, which interact with MSL1 via RING finger domain at its N-terminus. MSL2 is expressed only in Drosophila males. The DNA binding of the cysteine rich (CXC) domain is evolutionary conserved in human MSL2 ortholog (Lyman, et al., 1997). CXC domain is involved in the proper X-chromosomal target ...
... residues, which interact with MSL1 via RING finger domain at its N-terminus. MSL2 is expressed only in Drosophila males. The DNA binding of the cysteine rich (CXC) domain is evolutionary conserved in human MSL2 ortholog (Lyman, et al., 1997). CXC domain is involved in the proper X-chromosomal target ...
View PDF - DNA and Natural Algorithms Group
... and catalysts that would have preceded or mimicked those we see in modern metabolism. The process by which ancient catalysts may have arisen can be roughly reproduced in the laboratory. Random sequence RNA pools can be generated by a combination of chemical and enzymatic synthesis, and molecules tha ...
... and catalysts that would have preceded or mimicked those we see in modern metabolism. The process by which ancient catalysts may have arisen can be roughly reproduced in the laboratory. Random sequence RNA pools can be generated by a combination of chemical and enzymatic synthesis, and molecules tha ...
Genetic Biomarkers of Aging Drosophila Melanogaster Daria Solodovnikova
... are many theories of aging. One of them is the mitochondrial free radical theory of aging. Mitochondria-related aging may be associated with the generation of reactive oxygen species, the alteration of redox homeostasis, and the changes that happen in mitochondrial DNA, such as different point mutat ...
... are many theories of aging. One of them is the mitochondrial free radical theory of aging. Mitochondria-related aging may be associated with the generation of reactive oxygen species, the alteration of redox homeostasis, and the changes that happen in mitochondrial DNA, such as different point mutat ...
Gene Regulation Notes
... • MicroRNAs (miRNAs) are small singlestranded RNA molecules that can bind to mRNA • These miRNAs can degrade mRNA or block its ...
... • MicroRNAs (miRNAs) are small singlestranded RNA molecules that can bind to mRNA • These miRNAs can degrade mRNA or block its ...
TAR-RNA binding by HIV-1 Tat protein is
... nucleotides that interact with a nucleic acid binding peptide of HIV Tat (42). This and other published examples have proven that SELEX can be a powerful tool for the selection of nucleic acid sequences having very high affinity for a variety of biological molecules, including HIV-1 reverse transcri ...
... nucleotides that interact with a nucleic acid binding peptide of HIV Tat (42). This and other published examples have proven that SELEX can be a powerful tool for the selection of nucleic acid sequences having very high affinity for a variety of biological molecules, including HIV-1 reverse transcri ...
Identification and functional analysis of novel genes
... The analysis of the role of pleiotropic genes in germline development is rather difficult, but the RNAi technique provides an ideal tool since it enables the generation of a series of phenotypes of different strength. Using this approach, we were able to detect genes essential for germline developme ...
... The analysis of the role of pleiotropic genes in germline development is rather difficult, but the RNAi technique provides an ideal tool since it enables the generation of a series of phenotypes of different strength. Using this approach, we were able to detect genes essential for germline developme ...
Evidence for Repeat-Induced Gene Silencing in Cultured
... (gray box) [25] and Pgk-1 polyadenylation and transcription termination signals (black box) [26] flanking the lacZ-neo coding region [24]. LacZ-neo (also called geo) consists of a fusion between the coding regions of lacZ and the neomycin-resistance gene. The solid line represents the plasmid vecto ...
... (gray box) [25] and Pgk-1 polyadenylation and transcription termination signals (black box) [26] flanking the lacZ-neo coding region [24]. LacZ-neo (also called geo) consists of a fusion between the coding regions of lacZ and the neomycin-resistance gene. The solid line represents the plasmid vecto ...
Phylogenetic Affinity of Mitochondria of Euglena
... RNA molecules called guide RNAs mediate the uridine insertion/deletion type of RNA editing (Simpson et al. 1993). It is known that these guide RNA molecules can be capped in vitro with guanylyl transferase and GTP (Blum and Simpson 1990). To search for similar RNA species in E. gracilis mitochondria ...
... RNA molecules called guide RNAs mediate the uridine insertion/deletion type of RNA editing (Simpson et al. 1993). It is known that these guide RNA molecules can be capped in vitro with guanylyl transferase and GTP (Blum and Simpson 1990). To search for similar RNA species in E. gracilis mitochondria ...
video slide - Geneva High School
... ribosomes (attached to the ER) • Free ribosomes mostly synthesize proteins that function in the cytosol • Bound ribosomes make proteins of the endomembrane system and proteins that are secreted from the cell • Ribosomes are identical and can switch from free to bound Copyright © 2008 Pearson Educati ...
... ribosomes (attached to the ER) • Free ribosomes mostly synthesize proteins that function in the cytosol • Bound ribosomes make proteins of the endomembrane system and proteins that are secreted from the cell • Ribosomes are identical and can switch from free to bound Copyright © 2008 Pearson Educati ...
Par-1
... Components of RNAi machinery Many components of RNAi machinery have been identified through genetic screening for RNAi defective mutants and through biochemical studies using cell extracts (e.g. Drosophila embryo extract). ...
... Components of RNAi machinery Many components of RNAi machinery have been identified through genetic screening for RNAi defective mutants and through biochemical studies using cell extracts (e.g. Drosophila embryo extract). ...
mRNA
... • Of the 64 triplets, 61 code for amino acids; 3 triplets are “stop” signals to end translation • The genetic code is redundant but not ambiguous; no codon specifies more than one amino acid • Codons must be read in the correct reading frame (correct groupings) in order for the specified polypeptide ...
... • Of the 64 triplets, 61 code for amino acids; 3 triplets are “stop” signals to end translation • The genetic code is redundant but not ambiguous; no codon specifies more than one amino acid • Codons must be read in the correct reading frame (correct groupings) in order for the specified polypeptide ...
1st set of Journal Clubs this Wednesday!
... Prediction: If protein Gilbert and Müller-Hill isolated really was the lac repressor then it should bind to the lac operator in an inducer sensitive manner (i.e. the addition of inducer should prevent the repressor from binding to the operator). Experiment: Cohn and colleagues used a nitrocellulose ...
... Prediction: If protein Gilbert and Müller-Hill isolated really was the lac repressor then it should bind to the lac operator in an inducer sensitive manner (i.e. the addition of inducer should prevent the repressor from binding to the operator). Experiment: Cohn and colleagues used a nitrocellulose ...
Against the Central Dogma
... The Disease-Related Human LncProfiler kit contains 83 primer sets for studying human LncRNAs, along with 12 primer sets for internal controls and housekeeping genes. Each primer set has been designed according to several individual peer-reviewed literature references that demonstrate the importance ...
... The Disease-Related Human LncProfiler kit contains 83 primer sets for studying human LncRNAs, along with 12 primer sets for internal controls and housekeeping genes. Each primer set has been designed according to several individual peer-reviewed literature references that demonstrate the importance ...
pdf
... which is a melting or unwinding of about 12 bp. c. The initiating nucleotides can bind to the enzyme, as directed by their complementary nucleotides in the DNA template strand, and the enzyme will catalyze formation of a phosphodiester bond between them. This polymerase-DNA-RNA complex is referred t ...
... which is a melting or unwinding of about 12 bp. c. The initiating nucleotides can bind to the enzyme, as directed by their complementary nucleotides in the DNA template strand, and the enzyme will catalyze formation of a phosphodiester bond between them. This polymerase-DNA-RNA complex is referred t ...