Application of Bruchin B to pea pods results in
... been derived from a gene that was up-regulated by bruchin treatment, with stronger up-regulation in Np/Np pods than in np/np pods. After cloning and sequencing, the length of the GT13 clone was 710 bp, which corresponded to the size of the band observed on the DD gels. The sequence of the GT13 cDNA ...
... been derived from a gene that was up-regulated by bruchin treatment, with stronger up-regulation in Np/Np pods than in np/np pods. After cloning and sequencing, the length of the GT13 clone was 710 bp, which corresponded to the size of the band observed on the DD gels. The sequence of the GT13 cDNA ...
Nucleic Acid Structures, Energetics, and Dynamics
... reports on the structure of nucleic acids. The first, by Watson and Crick,1 proposed that DNA was a double helix of antiparallel strands held together by hydrogen bonds between complementary bases: guanine and cytosine or adenine and thymine. Their model followed from the fact that this was the only ...
... reports on the structure of nucleic acids. The first, by Watson and Crick,1 proposed that DNA was a double helix of antiparallel strands held together by hydrogen bonds between complementary bases: guanine and cytosine or adenine and thymine. Their model followed from the fact that this was the only ...
molecular biology
... – RNA nucleotides contain ribose while DNA contains deoxyribose (a type of ribose that lacks one oxygen atom) – in RNA the nucleotide uracil substitutes for thymine, which is present in DNA Machine Learning & Bioinformatics ...
... – RNA nucleotides contain ribose while DNA contains deoxyribose (a type of ribose that lacks one oxygen atom) – in RNA the nucleotide uracil substitutes for thymine, which is present in DNA Machine Learning & Bioinformatics ...
Presentation 1 Guidelines
... C4. The building blocks of a nucleotide are a sugar (ribose or deoxyribose), a nitrogenous base, and phosphate. In a nucleotide, the phosphate is already linked to the 5 position on the sugar. When two nucleotides are hooked together, a phosphate on one nucleotide forms a covalent bond with a hydro ...
... C4. The building blocks of a nucleotide are a sugar (ribose or deoxyribose), a nitrogenous base, and phosphate. In a nucleotide, the phosphate is already linked to the 5 position on the sugar. When two nucleotides are hooked together, a phosphate on one nucleotide forms a covalent bond with a hydro ...
Supplementary Information (doc 82K)
... Table 1). The SS-cDNA was prepared using forward primers for antisense RNA and reverse primers for sense RNA against 18S rRNA (298 bp), 5.8S rRNA (262 bp) and LSU α (28S α) rRNA (266 bp). Arrow marks indicate amplified fragments obtained for respective sense and antisense RNA. -RT, cDNA was prepared ...
... Table 1). The SS-cDNA was prepared using forward primers for antisense RNA and reverse primers for sense RNA against 18S rRNA (298 bp), 5.8S rRNA (262 bp) and LSU α (28S α) rRNA (266 bp). Arrow marks indicate amplified fragments obtained for respective sense and antisense RNA. -RT, cDNA was prepared ...
plantcell.org - Schnable Lab
... from companion cells labeled by the expression of green fluorescent protein (Brandt et al., 1999). However, a disadvantage of this method is that it requires the use of cell-specific promoters, and relatively few such promoters are available. A second way to collect specific cell types is to use pro ...
... from companion cells labeled by the expression of green fluorescent protein (Brandt et al., 1999). However, a disadvantage of this method is that it requires the use of cell-specific promoters, and relatively few such promoters are available. A second way to collect specific cell types is to use pro ...
SN1 Question Paper Sum 2007
... Answer ALL SEVEN questions in the spaces provided in this booklet. If you need to use additional answer sheets, attach them loosely but securely inside this booklet. Show all the steps in any calculations and state the units. Calculators may be used. Include diagrams in your answers where these are ...
... Answer ALL SEVEN questions in the spaces provided in this booklet. If you need to use additional answer sheets, attach them loosely but securely inside this booklet. Show all the steps in any calculations and state the units. Calculators may be used. Include diagrams in your answers where these are ...
Reverse Transcriptase and cDNA Synthesis
... previously established the focus assay system for transformation by RSV. This work made it possible to titrate an amount of the virus by counting the number of foci. Using this technique, he analyzed replication of the virus and made a series of unexpected observations indicating that the replicatio ...
... previously established the focus assay system for transformation by RSV. This work made it possible to titrate an amount of the virus by counting the number of foci. Using this technique, he analyzed replication of the virus and made a series of unexpected observations indicating that the replicatio ...
Intracellular Distribution of Radioactivity in Nucleic Acid tration of
... various thicknesses to differentiate the C―and P―.By means of empirical factors, the specific activity the protein was calculated. ...
... various thicknesses to differentiate the C―and P―.By means of empirical factors, the specific activity the protein was calculated. ...
Telomerase Is a True Reverse Transcriptase
... How, then, is the chromosome end maintained? The molecular basis of telomere replication came to light in 1985 with the discovery by Greider and Blackburn of the enzyme telomere terminal transferase or telomerase in Tetrahymena thermophila [10]. They later showed that telomerase was a ribonucleoprot ...
... How, then, is the chromosome end maintained? The molecular basis of telomere replication came to light in 1985 with the discovery by Greider and Blackburn of the enzyme telomere terminal transferase or telomerase in Tetrahymena thermophila [10]. They later showed that telomerase was a ribonucleoprot ...
Central Dogma of Molecular Biology Nucleotide Structure
... translated. The mRNA population in a cell is very heterogeneous in size and base sequence, as the cell has essentially a different mRNA molecule for each of the thousands of different proteins made by that cell. 4. Heterogeneous nuclear RNA (hnRNA or pre-mRNA), which is found only in the nucleus of ...
... translated. The mRNA population in a cell is very heterogeneous in size and base sequence, as the cell has essentially a different mRNA molecule for each of the thousands of different proteins made by that cell. 4. Heterogeneous nuclear RNA (hnRNA or pre-mRNA), which is found only in the nucleus of ...
Effective Controls for RNA Interference (RNAi) Experiments using
... target gene is expressed in all cell types at a level that does not fluctuate with cell cycle. An abundantly expressed gene allows easy and accurate assay of its mRNA and protein levels. However, silencing of the gene should not affect either the cell phenotype or its viability. Three popular target ...
... target gene is expressed in all cell types at a level that does not fluctuate with cell cycle. An abundantly expressed gene allows easy and accurate assay of its mRNA and protein levels. However, silencing of the gene should not affect either the cell phenotype or its viability. Three popular target ...
Ribosomes: Cashing in on crystals
... density between the bound and free ribosome crystals. The overall architecture of the 70S ribosome seen here is similar to that observed previously by cryo-electron microscopy. Furthermore, structural features seen in the 30S and 50S electron density maps are also observed, such as the long rRNA hel ...
... density between the bound and free ribosome crystals. The overall architecture of the 70S ribosome seen here is similar to that observed previously by cryo-electron microscopy. Furthermore, structural features seen in the 30S and 50S electron density maps are also observed, such as the long rRNA hel ...
Transcription regulation of the Escherichia coli pcnB gene coding for
... There are two major eVectors of the stringent response. The Wrst one is a couple of two speciWc nucleotides, guanosine tetraphosphate (ppGpp) and guanosine pentaphosphate (pppGpp), collectively called (p)ppGpp and rapidly produced in response to variety of physico-chemical and nutritional stresses ( ...
... There are two major eVectors of the stringent response. The Wrst one is a couple of two speciWc nucleotides, guanosine tetraphosphate (ppGpp) and guanosine pentaphosphate (pppGpp), collectively called (p)ppGpp and rapidly produced in response to variety of physico-chemical and nutritional stresses ( ...
Host-pathogen interactions in drosophila: new tricks from an old friend
... Another family of insect proteins thought to function as opsonins are the Teps (thioester-containing proteins). The drosophila genome includes six potential Teps, all of which include several domains common to the α2-macroglobulin and complement (C3) family. These proteins form covalent adducts with ...
... Another family of insect proteins thought to function as opsonins are the Teps (thioester-containing proteins). The drosophila genome includes six potential Teps, all of which include several domains common to the α2-macroglobulin and complement (C3) family. These proteins form covalent adducts with ...
The RNA world meets behavior: AfiI pre
... that can be generated from an edited gene. Another difference between editing and normal genetic variation is the potential for spatial and temporal regulation. Because an ADAR enzyme introduces modifications into mRNAs, editing is thus dependent on the amount and location of that protein. Indeed, d ...
... that can be generated from an edited gene. Another difference between editing and normal genetic variation is the potential for spatial and temporal regulation. Because an ADAR enzyme introduces modifications into mRNAs, editing is thus dependent on the amount and location of that protein. Indeed, d ...
Chapter 17--6 slides per page
... The information content of genes is in the specific sequences of nucleotides The DNA inherited by an organism leads to specific traits by dictating the synthesis of proteins Thus, proteins are the links between genotype and phenotype Gene expression, the process by which DNA directs protein ...
... The information content of genes is in the specific sequences of nucleotides The DNA inherited by an organism leads to specific traits by dictating the synthesis of proteins Thus, proteins are the links between genotype and phenotype Gene expression, the process by which DNA directs protein ...
Genes
... cells and epithelial cells. It occurs during growth, replacement, or repair. Cell division that forms gametes is termed meiosis and occurs only once in a cell line. ...
... cells and epithelial cells. It occurs during growth, replacement, or repair. Cell division that forms gametes is termed meiosis and occurs only once in a cell line. ...
RNA-binding proteins
... RNA-binding proteins (RBPs) play key roles in post-transcriptional control of RNAs, which, along with transcriptional regulation, is a major way to regulate patterns of gene expression during development. Post-transcriptional control can occur at many different steps in RNA metabolism, including spl ...
... RNA-binding proteins (RBPs) play key roles in post-transcriptional control of RNAs, which, along with transcriptional regulation, is a major way to regulate patterns of gene expression during development. Post-transcriptional control can occur at many different steps in RNA metabolism, including spl ...
On the energy and material cost of gene duplication
... The material cost of gene expression in the yeast Saccharomyces cerevisiae Some elemental nutrients are major components of the biomass produced in gene expression. Specifically, RNA contains carbon, nitrogen, and phosphorus. Protein contains carbon, nitrogen, and sulfur. Nutrients such as nitrogen ...
... The material cost of gene expression in the yeast Saccharomyces cerevisiae Some elemental nutrients are major components of the biomass produced in gene expression. Specifically, RNA contains carbon, nitrogen, and phosphorus. Protein contains carbon, nitrogen, and sulfur. Nutrients such as nitrogen ...
Handbook for Azospirillum
... 4.1.3 Transposon Mutagenesis and Gene Knockout Classical methods of bacterial mutagenesis such as chemical treatment or UV irradiation have been successfully employed in Azospirillum (examples are given in Elmerich 1983; Del Gallo et al. 1985; Holguin et al. 1999). However, mutated genes are more ea ...
... 4.1.3 Transposon Mutagenesis and Gene Knockout Classical methods of bacterial mutagenesis such as chemical treatment or UV irradiation have been successfully employed in Azospirillum (examples are given in Elmerich 1983; Del Gallo et al. 1985; Holguin et al. 1999). However, mutated genes are more ea ...
From Genes to Proteins
... should see that many three-letter combinations can be produced. Compare this exercise to the table in Figure 4. Ask students how the two compare. (In the exercise, letters are combined to form words, but in the table the letters stand for combinations of ...
... should see that many three-letter combinations can be produced. Compare this exercise to the table in Figure 4. Ask students how the two compare. (In the exercise, letters are combined to form words, but in the table the letters stand for combinations of ...