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Cloning and characterization in Escherichia coli of the gene
Cloning and characterization in Escherichia coli of the gene

... 50 Wg ampicillin per ml. The preculture was transferred to 200 ml of the fresh 2UYT medium containing 1 mM isopropyl-L-D-thiogalactopyranoside and 50 Wg ampicillin per ml, and cultured for 15 h at 37³C. The cells were harvested by centrifugation, suspended in 10 ml potassium phosphate bu¡er (pH 7.0) ...
K -Channel Transgenes Reduce K Currents in Paramecium
K -Channel Transgenes Reduce K Currents in Paramecium

... protein that causes the hyperexcitable phenotype. Accordingly, we engineered a pPXV plasmid with a single base-pair insertion downstream of the start codon of the PAK11 ORF, frameshifted (fs) pPXV-PAK11, and tested for its transformation effect. This insertion (5⬘ ATGATAC . . . to 5⬘ ATGATAAC . . .) ...
Gene Expression of Heart and Adipocyte Fatty Acid
Gene Expression of Heart and Adipocyte Fatty Acid

... IMF content and abdominal fat are shown in Table 3. The correlation coefficients between H-FABP gene mRNA and cardiac muscle, breast muscle and leg muscle were -0.749, -0.668 and -0.723 respectively. H-FABP gene mRNA expression level was significantly negative correlated with IMF content. Moreover, ...
Origins of Life - My George School
Origins of Life - My George School

... http://www.sciencechannel.com/tv-shows/greatest-discoveries/videos/100greatest-discoveries-origin-of-life/ ...
in plant physiology
in plant physiology

... group of tRNA genes, but not photosynthesis genes (Kanamaru et al., 2001), while SIG6 is essential for the transcription of a wide range of photosynthesis-related genes at an early stage of chloroplast development (Ishizaki et al., 2005). It seems likely that SIG2 and SIG6 work in cooperation durin ...
Protein Synthesis
Protein Synthesis

...  That’s all there is to it. When you get to the end, just right click or click on the arrow here, and then click on ‘End Show’,  If you don’t see the arrow, move the mouse and it will appear! ...
module 3: transcription part ii
module 3: transcription part ii

... using a chemical method to tag the special structure that occurs at 5’ ends of transcript, fishing out the RNA molecules using these tags, and mapping the sequence back to the genome, a method called “CAGE” (cap analysis of gene expression). In addition, we will also display the "D. mel. cDNA" track ...
Document
Document

... A. coding sequences - eukaryotes have introns within their coding sequence B. promoter C. enhancers – found in eukaryotes D. UTRs – found in eukaryotes E. poly-adenylation sequence – found within the eukaryotic 3’ UTR ...
Figure 2 - GEP Community Server
Figure 2 - GEP Community Server

Genomic characterization and phylogenetic analysis
Genomic characterization and phylogenetic analysis

... CSBV is similar to SBV-UK in its physiological and biochemical features, but differs in antigenicity and do not show cross-infection. Sequence analysis indicated that CSBV has some differences, but is highly homologous to SBV-UK (Zhang et al., 2001). The CSBV genome is composed of a positive single- ...
Monday March 10th in-class “assignment”: studying SRS and BWS
Monday March 10th in-class “assignment”: studying SRS and BWS

... -Trends: BWS and SRS facial characteristics show opposite phenotype. Other explanations for the differences in phenotypes could be: environmental, or differences in other areas of the genome. 2. The authors report using OMIM to obtain some information for their research. Take a few minutes to look u ...
published a paper
published a paper

... lane 5). Since previously isolated ribozymes from our laboratory also use PRPP as a substrate for 6SGMP synthesis, it was possible that the PRPP product resulted from cross-contamination with these ribozymes. To eliminate this possibility, the pR1PR products and pR1-PRPP product were purified separa ...
NIH Public Access
NIH Public Access

... repeats (identified as repeat A, B, C, D). Studies of Xist RNA transgenes in mouse ES cells suggest that the A-repeat region is required for Xist RNA to silence chromatin (Wutz et al., 2002) and is important for PRC2 (EZH2) recruitment to enact H3K27 methylation (Zhao et al., 2008). With respect to ...
Infectious Pancreatic Necrosis Virus
Infectious Pancreatic Necrosis Virus

... amplification, forward and reverse primers hybridize to the IPNV cDNA. A fluorogenic probe is included in the same reaction mixture which consists of a DNA probe labeled with a 5`-dye and a 3`-quencher. During PCR amplification, the probe is cleaved and the reporter dye and quencher are separated. T ...
Brian Yu - Comparison of Microarrayc and RNA-Seq Analysis Methods for Single Cell Transcriptiomics
Brian Yu - Comparison of Microarrayc and RNA-Seq Analysis Methods for Single Cell Transcriptiomics

... Independent component analysis (ICA) is another method to extract biological significant dimensions from microarray data. Compared to SVD, ICA assumes non-Gaussian expression variations and models the microarray observations as a linear combination of its components, which are chosen to be as indepe ...
AP Lesson #50 After transcription, do prokaryotes need to modify
AP Lesson #50 After transcription, do prokaryotes need to modify

... – Eukaryotic genes have junk DNA! – Average gene contains 27,000 bases, of which about 1,200 bases actually code for proteins ...
File
File

... No protein is required to physically remove the RNA from the DNA This type of termination is also called intrinsic ...
Chapter 06 Lecture PowerPoint - McGraw Hill Higher Education
Chapter 06 Lecture PowerPoint - McGraw Hill Higher Education

... • There are 2 main types of terminators – Intrinsic terminators function with the RNA polymerase by itself without help from other proteins – Other type depends on auxiliary factor called rho (r, these are rho or r-dependent terminators ...
Document
Document

... Biochemical and Structural Characterization of RNA Modifying Enzymes RNA Uridine methyltransferase, RumA – Biochemical characterization of a RNA Uridine Methylatransfearse, RumA was done. This Methyltransferase contains a 4Fe-4S cluster and we are currently studying the role of this cluster. We have ...
Supporting Information Legends Supporting Figure 1. Amino acid
Supporting Information Legends Supporting Figure 1. Amino acid

... sequence ...
Giant chromosomes
Giant chromosomes

... • The paired chromosomes of oocytes in meiosis consist of numerous chromatin loops arranged along an axis . Chiasma formation is visible at various locations. • Each segment of a lampbrush chromosome consists of a series of chromatin loops, originating from an axis and a condensed structure, the chr ...
Biochimica et Biophysica Acta
Biochimica et Biophysica Acta

... contains a modified lacZ reporter gene with an RNA-binding element located close to the translation initiation region. A second plasmid expresses the RNA-binding protein that recognizes this RNA element. When the protein is bound to its cognate RNA located on the reporter mRNA, ribosomal assembly is ...
Gill: Transcription Regulation I
Gill: Transcription Regulation I

Can the Origin of the Genetic Code Be Explained - BIO
Can the Origin of the Genetic Code Be Explained - BIO

... proteins recognizes a specific amino acid and the specific anticodons it binds to within the code. They then bind amino acids to the tRNA that bears the corresponding anticodon. Thus, instead of the code reducing to a simple set of stereochemical affinities, biochemists have found a functionally int ...
Human microRNA target analysis and gene ontology clustering by
Human microRNA target analysis and gene ontology clustering by

... and the experimental one, TarBase. An intersection analysis between each prediction database and TarBase was performed by calculating the total number of common targets between each prediction database and TarBase for all the miRNAs available in TarBase (Figure 2A). TargetScan predictions, as docume ...
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RNA silencing

RNA silencing (associated with the concept of post-transcriptional gene silencing or RNA interference) refers to a family of gene silencing effects by which the expression of one or more genes is downregulated or entirely suppressed by non-coding RNAs, particularly small RNAs. It may also refer to the introduction of a synthetic antisense RNA molecule used in scientific experiments on gene expression. RNA silencing may also be defined as sequence-specific regulation of gene expression triggered by double-stranded RNA (dsRNA). RNA silencing mechanisms are highly conserved in most eukaryotes. The most common and well-studied example is RNA interference (RNAi), in which endogenously expressed microRNA (miRNA) or exogenously derived small interfering RNA (siRNA) induces the degradation of complementary messenger RNA. Other classes of small RNA have been identified, including piwi-interacting RNA (piRNA) and its subspecies repeat associated small interfering RNA (rasiRNA).
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