KIN241: a gene involved in cell morphogenesis in
... single cell, the egg, it would not be surprising that some of the mechanisms that play a role in ciliate morphogenesis might have been conserved and be involved in early steps of metazoan development. Here, we report the characterization of a gene, KIN241, identified by a morphological mutation in P ...
... single cell, the egg, it would not be surprising that some of the mechanisms that play a role in ciliate morphogenesis might have been conserved and be involved in early steps of metazoan development. Here, we report the characterization of a gene, KIN241, identified by a morphological mutation in P ...
Foundations of Biology - Geoscience Research Institute
... cells all the time. These continually expressed genes are called constitutive genes. Other genes are only needed by certain cells or at specific times. The expression of these inducible genes is tightly controlled. For example, pancreas beta cells make the protein insulin by expressing the insul ...
... cells all the time. These continually expressed genes are called constitutive genes. Other genes are only needed by certain cells or at specific times. The expression of these inducible genes is tightly controlled. For example, pancreas beta cells make the protein insulin by expressing the insul ...
translational - Bioinformatics Institute
... • Translation takes place on defined cytoplasmic organelles called RIBOSOMES. ...
... • Translation takes place on defined cytoplasmic organelles called RIBOSOMES. ...
video slide - SharpSchool
... arginine in their growth medium and had shown genetically that these mutants fell into three classes, each defective in a different gene. From other considerations, they suspected that the metabolic pathway of arginine biosynthesis included the precursors ornithine and citrulline. Their most famous ...
... arginine in their growth medium and had shown genetically that these mutants fell into three classes, each defective in a different gene. From other considerations, they suspected that the metabolic pathway of arginine biosynthesis included the precursors ornithine and citrulline. Their most famous ...
Identification of three MADS‐box genes expressed in sunflower
... As a ®rst step towards this goal, MADS-box containing cDNAs were cloned from sun¯ower R2-stage in¯orescence RNA by RTPCR using degenerated oligonucleotides deduced from conserved regions of members of the AG and DEF groups. Only one expressed gene could be identi®ed with oligonucleotides for the AG ...
... As a ®rst step towards this goal, MADS-box containing cDNAs were cloned from sun¯ower R2-stage in¯orescence RNA by RTPCR using degenerated oligonucleotides deduced from conserved regions of members of the AG and DEF groups. Only one expressed gene could be identi®ed with oligonucleotides for the AG ...
Plant Virology
... • Loss due to plant viruses is often difficult to quantify, but they are often of great importance as plant pathogens (fungi are most economically important ) • Viruses do not usually kill plants, and symptoms on plants are often subtle • Virus diseases of plants are not subject to chemical control ...
... • Loss due to plant viruses is often difficult to quantify, but they are often of great importance as plant pathogens (fungi are most economically important ) • Viruses do not usually kill plants, and symptoms on plants are often subtle • Virus diseases of plants are not subject to chemical control ...
RNA PCR Kit (AMV)
... amplify RNA. However, synthesis of cDNA from RNA using reverse transcriptase enables to apply PCR process to the RNA analysis. Many reports of various fields have been made by applying this method, such as of structual analysis of RNA, efficient cDNA cloning, analysis of gene expression at the RNA l ...
... amplify RNA. However, synthesis of cDNA from RNA using reverse transcriptase enables to apply PCR process to the RNA analysis. Many reports of various fields have been made by applying this method, such as of structual analysis of RNA, efficient cDNA cloning, analysis of gene expression at the RNA l ...
Formation and nuclear export of tRNA, rRNA and mRNA is regulated
... reduced in the rsp5-3 strain during incubation at 37 1C, but this decline was much slower than that seen for the pre-rRNAs, suggesting that it may be an indirect effect of the growth arrest. The defective nuclear RNA export in rsp5-3 may be due to an inhibition of components of the respective RNA bi ...
... reduced in the rsp5-3 strain during incubation at 37 1C, but this decline was much slower than that seen for the pre-rRNAs, suggesting that it may be an indirect effect of the growth arrest. The defective nuclear RNA export in rsp5-3 may be due to an inhibition of components of the respective RNA bi ...
SafeView - NBS Biologicals
... Staining solution may be stored at room temperature and reused. SafeView is non-carcinogenic but may cause skin and eye irritations. Always wear gloves when working with the product. This product is distributed for laboratory research only. CAUTION: Not for diagnostic use. The safety and efficiency ...
... Staining solution may be stored at room temperature and reused. SafeView is non-carcinogenic but may cause skin and eye irritations. Always wear gloves when working with the product. This product is distributed for laboratory research only. CAUTION: Not for diagnostic use. The safety and efficiency ...
Effect of Adriamycin on DNA, RNA, and Protein Synthesis in Cell
... DNA polymerase reaction is shown in Table 1. When the hypotonicity of the cell suspension was adjusted to isoto concentration of denatured DNA in the reaction mixture was nicity by addition of an equal volume of Buffer B (40 mM Tnis-HCI, pH 7.5; 10 mM potassium phosphate buffer, pH 100 jig/mI, the c ...
... DNA polymerase reaction is shown in Table 1. When the hypotonicity of the cell suspension was adjusted to isoto concentration of denatured DNA in the reaction mixture was nicity by addition of an equal volume of Buffer B (40 mM Tnis-HCI, pH 7.5; 10 mM potassium phosphate buffer, pH 100 jig/mI, the c ...
Chapter 14
... pN is an antitermination factor that allows RNA polymerase to continue transcription past the ends of the two immediate early genes. pQ is the product of a delayed early gene and is an antiterminator that allows RNA polymerase to transcribe the late genes. Lambda DNA circularizes after infection; as ...
... pN is an antitermination factor that allows RNA polymerase to continue transcription past the ends of the two immediate early genes. pQ is the product of a delayed early gene and is an antiterminator that allows RNA polymerase to transcribe the late genes. Lambda DNA circularizes after infection; as ...
NuGEN Introduces Target Enrichment Technology for
... “Screening samples for a broad range of gene fusions has been a costly and time consuming process until now,” commented Doug Amorese, VP of R&D at NuGEN. “The SPET technology for targeted RNA sequencing enables NGS analysis to identify any fusion event in the targeted gene set and will be an importa ...
... “Screening samples for a broad range of gene fusions has been a costly and time consuming process until now,” commented Doug Amorese, VP of R&D at NuGEN. “The SPET technology for targeted RNA sequencing enables NGS analysis to identify any fusion event in the targeted gene set and will be an importa ...
transcription factor
... and a very small fraction of the non-protein-coding DNA consists of genes for RNA such as rRNA and tRNA • A significant amount of the genome may be transcribed into noncoding RNAs (ncRNAs) • Noncoding RNAs regulate gene expression at two points: mRNA translation and chromatin configuration ...
... and a very small fraction of the non-protein-coding DNA consists of genes for RNA such as rRNA and tRNA • A significant amount of the genome may be transcribed into noncoding RNAs (ncRNAs) • Noncoding RNAs regulate gene expression at two points: mRNA translation and chromatin configuration ...
Recombination in large RNA viruses: Coronaviruses
... Using this approach, recombinant viruses which had incorporated DI sequences into the viral RNA could be obtained at a higher efficiency than when nonreplicating RNAs were used.34,35,39 Theoretically, either the 5'- or 3'-end sequences of the DI RNAs could be incorporated into the viral RNA via this ...
... Using this approach, recombinant viruses which had incorporated DI sequences into the viral RNA could be obtained at a higher efficiency than when nonreplicating RNAs were used.34,35,39 Theoretically, either the 5'- or 3'-end sequences of the DI RNAs could be incorporated into the viral RNA via this ...
PART I
... gene transcripts. The false positive rate with DDRT-PCR can be highly variable and therefore it can be best used as a screening procedure rather than a cloning strategy. A similar RT-PCR screening technique called RAP-PCR (RNA arbitrarily primed PCR), is based on a genomic DNA fingerprinting strateg ...
... gene transcripts. The false positive rate with DDRT-PCR can be highly variable and therefore it can be best used as a screening procedure rather than a cloning strategy. A similar RT-PCR screening technique called RAP-PCR (RNA arbitrarily primed PCR), is based on a genomic DNA fingerprinting strateg ...
Separation of Nucleic acid constituents Nucleic acids do exist in
... (5'-AMP), 5'-guanylic acid (5'-GMP), 5'-cytidylic acid (5'-CMP) and 5'-uridylic acid (5'-UMP), and 5'-AMP is then converted into 5'-inosinic acid (5'-IMP). ( Please see Fig.VIII-7-2. ) 5'-GMP and 5'-IMP have not only the taste of shiitake mushroom and the one of dried bonito respectively but also sy ...
... (5'-AMP), 5'-guanylic acid (5'-GMP), 5'-cytidylic acid (5'-CMP) and 5'-uridylic acid (5'-UMP), and 5'-AMP is then converted into 5'-inosinic acid (5'-IMP). ( Please see Fig.VIII-7-2. ) 5'-GMP and 5'-IMP have not only the taste of shiitake mushroom and the one of dried bonito respectively but also sy ...
12_ Nucleic Acids
... The secondary structure of DNA was proposed by James Watson and Francis Crick in 1953. This was perhaps the greatest discovery of modern biology and one of the most remarkable and profound events in the history of science. Watson and Crick concluded that DNA is a double helix containing two polynucl ...
... The secondary structure of DNA was proposed by James Watson and Francis Crick in 1953. This was perhaps the greatest discovery of modern biology and one of the most remarkable and profound events in the history of science. Watson and Crick concluded that DNA is a double helix containing two polynucl ...
12.1 Components of Nucleic Acids
... The secondary structure of DNA was proposed by James Watson and Francis Crick in 1953. This was perhaps the greatest discovery of modern biology and one of the most remarkable and profound events in the history of science. Watson and Crick concluded that DNA is a double helix containing two polynucl ...
... The secondary structure of DNA was proposed by James Watson and Francis Crick in 1953. This was perhaps the greatest discovery of modern biology and one of the most remarkable and profound events in the history of science. Watson and Crick concluded that DNA is a double helix containing two polynucl ...
Gene regulation I Biochemistry 302
... • Dictated by changes in RNA secondary structure • Extends the possible range of transcription rates (moderate to high Trp levels) ...
... • Dictated by changes in RNA secondary structure • Extends the possible range of transcription rates (moderate to high Trp levels) ...
Promega Enzyme Resource Guide, Cloning Enzymes , BR075B
... ligase for ligation of single-strand breaks or joining of DNA molecules with cohesive termini. However, unlike T4 DNA ligase, the E. coli enzyme does not show blunt-ended ligation activity, except under conditions of molecular crowding with PEG 8000 (5). For this reason T4 DNA ligase has become more ...
... ligase for ligation of single-strand breaks or joining of DNA molecules with cohesive termini. However, unlike T4 DNA ligase, the E. coli enzyme does not show blunt-ended ligation activity, except under conditions of molecular crowding with PEG 8000 (5). For this reason T4 DNA ligase has become more ...
RNA aptamers as genetic control devices: The potential of
... eukaryotes and mammalian cell lines for higher eukaryotes [19]. In developmental biology, biotechnology and in medicine, other organisms are frequently employed as physiological, developmental and disease models or as cell factories for the production of materials [75–80]. Since endogenous riboswitc ...
... eukaryotes and mammalian cell lines for higher eukaryotes [19]. In developmental biology, biotechnology and in medicine, other organisms are frequently employed as physiological, developmental and disease models or as cell factories for the production of materials [75–80]. Since endogenous riboswitc ...
