Supplementary Data 1 (doc 909K)
... recognizes ECOP in SCC-9, a squamous cell carcinoma derived cell line, as two protein species of 21.2 and 19.7 kD (supplemental data). The proteins recognized by this antiserum are specifically reduced by siRNA oligonucleotides directed against the ECOP transcript, but not by scrambled oligonucleoti ...
... recognizes ECOP in SCC-9, a squamous cell carcinoma derived cell line, as two protein species of 21.2 and 19.7 kD (supplemental data). The proteins recognized by this antiserum are specifically reduced by siRNA oligonucleotides directed against the ECOP transcript, but not by scrambled oligonucleoti ...
A Penetrating Look at stochasticity in Development
... the cytoplasm, where Dicer generates ?21 nucleotide double-stranded RNA intermediates. Such double-stranded RNAs are processed further, and one strand, the mature miRNA, interacts with Argonaute (Ago) proteins to form miRNA-protein complexes (miRNPs) (Bartel, 2009; Carthew and Sontheimer, 2009). miR ...
... the cytoplasm, where Dicer generates ?21 nucleotide double-stranded RNA intermediates. Such double-stranded RNAs are processed further, and one strand, the mature miRNA, interacts with Argonaute (Ago) proteins to form miRNA-protein complexes (miRNPs) (Bartel, 2009; Carthew and Sontheimer, 2009). miR ...
Von Neumann`s Quintessential Message: Genotype C Ribotype D
... In 1958, one year after John von Neumann’s death, two major events took place in the history of molecular biology: First, Francis Crick, one of the discoverers of the DNA double helix, put forward what he called the central dogma of molecular biology: Proteins are not made directly from genes—there ...
... In 1958, one year after John von Neumann’s death, two major events took place in the history of molecular biology: First, Francis Crick, one of the discoverers of the DNA double helix, put forward what he called the central dogma of molecular biology: Proteins are not made directly from genes—there ...
Nucleic acids
... • A nucleoside consists of a nitrogen base linked by a glycosidic bond to C1’ of a ribose or deoxyribose • Nucleosides are named by changing the nitrogen base ending to -osine for purines and –idine for pyrimidines • A nucleotide is a nucleoside that forms a phosphate ester with the C5’ OH group of ...
... • A nucleoside consists of a nitrogen base linked by a glycosidic bond to C1’ of a ribose or deoxyribose • Nucleosides are named by changing the nitrogen base ending to -osine for purines and –idine for pyrimidines • A nucleotide is a nucleoside that forms a phosphate ester with the C5’ OH group of ...
![easy-spinTM [DNA free] Total RNA Extraction Kit](http://s1.studyres.com/store/data/022411047_1-c79ddf09c383916f41257db55a59cdba-300x300.png)
easy-spinTM [DNA free] Total RNA Extraction Kit
... 10sec), and add 1ml of Lysis buffer(easy-BLUETM reagent). Note : In case of adherent cell, measure the viable count after trypsin treatment. In case of suspended cell, measure the viable count after centrifugation. Although 1ml of Lysis Buffer is good for the preparation of up to 5-10x106 cell, it i ...
... 10sec), and add 1ml of Lysis buffer(easy-BLUETM reagent). Note : In case of adherent cell, measure the viable count after trypsin treatment. In case of suspended cell, measure the viable count after centrifugation. Although 1ml of Lysis Buffer is good for the preparation of up to 5-10x106 cell, it i ...
1.B.1 Conserved Core Processes
... Organisms share many conserved core processes and features that evolved and are widely distributed among organisms today. ...
... Organisms share many conserved core processes and features that evolved and are widely distributed among organisms today. ...
Update on FRDA Research
... (FXN) - 95% of abnormal alleles. The other 5% of abnormal alleles are point mutations in the coding regions of the gene. ONE DOES NOT HAVE TO PUT IN NEW GENE, ONLY TURN THAT WHICH THERE ON ...
... (FXN) - 95% of abnormal alleles. The other 5% of abnormal alleles are point mutations in the coding regions of the gene. ONE DOES NOT HAVE TO PUT IN NEW GENE, ONLY TURN THAT WHICH THERE ON ...
Origin of Life - stephen fleenor
... into polymers (DNA, RNA, proteins, and lipids) These molecules began self-replicating in water ...
... into polymers (DNA, RNA, proteins, and lipids) These molecules began self-replicating in water ...
A CAAT–Box Binding Factor Gene That Regulates Seed Development
... •Transcription factors are sequence-specific DNA binding factors proteins. They promote or block transcription by controlling the recruitment of RNA polymerase •Transcription is initiated at regions of DNA called promoters. Specific sequences of nucleotide bases at a promoter are recognized by both ...
... •Transcription factors are sequence-specific DNA binding factors proteins. They promote or block transcription by controlling the recruitment of RNA polymerase •Transcription is initiated at regions of DNA called promoters. Specific sequences of nucleotide bases at a promoter are recognized by both ...
Chapter 7A
... involved in lactose metabolism, uses both mechanisms of control (Fig. 7.3). A specific repressor protein (the lac repressor) inhibits transcription from the lac promoter by binding to an adjacent DNA sequence known as the lac operator in the absence of lactose. A general activator protein known as c ...
... involved in lactose metabolism, uses both mechanisms of control (Fig. 7.3). A specific repressor protein (the lac repressor) inhibits transcription from the lac promoter by binding to an adjacent DNA sequence known as the lac operator in the absence of lactose. A general activator protein known as c ...
OLSON LAB PROTOCOL: Working with RNA
... non-coding species, such as ribosomal RNA, transfer RNA and micro RNAs. These types of RNAs are not translated into proteins, but are nevertheless able to act as functional enzymes or substrates for gene translation or regulation. As such, they are expressed in effectively all cells at all times (co ...
... non-coding species, such as ribosomal RNA, transfer RNA and micro RNAs. These types of RNAs are not translated into proteins, but are nevertheless able to act as functional enzymes or substrates for gene translation or regulation. As such, they are expressed in effectively all cells at all times (co ...
Inquiry into Life Twelfth Edition
... • Structure of yeast polymerase II (specifically pol II 4/7) at atomic resolution reveals a deep cleft(갈라진 틈, 옴폭 들어간 부분) that accepts a linear DNA template from one end to another • Catalytic center lies at the bottom of the cleft and ...
... • Structure of yeast polymerase II (specifically pol II 4/7) at atomic resolution reveals a deep cleft(갈라진 틈, 옴폭 들어간 부분) that accepts a linear DNA template from one end to another • Catalytic center lies at the bottom of the cleft and ...
Gene Activity - Haiku Learning
... The Genetic Code The unit of a code consists of codons, each of which is a unique arrangement of symbols Each of the 20 amino acids found in proteins is uniquely specified by one or more codons The symbols used by the genetic code are the mRNA bases - Function as “letters” of the genetic alphabet ...
... The Genetic Code The unit of a code consists of codons, each of which is a unique arrangement of symbols Each of the 20 amino acids found in proteins is uniquely specified by one or more codons The symbols used by the genetic code are the mRNA bases - Function as “letters” of the genetic alphabet ...
Figure S2.
... Figure S2. NELF-E potentiates expression of the slp1[PESE]-lacZ reporter. Fluorescent double in situ hybridization was used to compare the expression of a reporter gene containing a slp1 cis-regulatory element extending from 3.9 to 1.8 kb upstream of the slp1 promoter fused to a 129 bp slp1 basal pr ...
... Figure S2. NELF-E potentiates expression of the slp1[PESE]-lacZ reporter. Fluorescent double in situ hybridization was used to compare the expression of a reporter gene containing a slp1 cis-regulatory element extending from 3.9 to 1.8 kb upstream of the slp1 promoter fused to a 129 bp slp1 basal pr ...
Appendix A: General Remarks on Handling RNA
... No currently available purification method can guarantee that RNA is completely free of DNA, even when it is not visible on an agarose gel. To prevent any interference by DNA in RT-PCR applications, we recommend designing primers that anneal at intron splice junctions so that genomic DNA will not be ...
... No currently available purification method can guarantee that RNA is completely free of DNA, even when it is not visible on an agarose gel. To prevent any interference by DNA in RT-PCR applications, we recommend designing primers that anneal at intron splice junctions so that genomic DNA will not be ...
2 Biogenesis and the regulation of the maturation of miRNAs
... RNAs) and were implicated in Caenorhabditis elegans development [1]. In subsequent studies these small RNAs were shown to be common and abundant in plants and animals [2–4], but do not exist in the bacterial kingdom. They are now proven to be the key regulators of gene expression in virtually all bi ...
... RNAs) and were implicated in Caenorhabditis elegans development [1]. In subsequent studies these small RNAs were shown to be common and abundant in plants and animals [2–4], but do not exist in the bacterial kingdom. They are now proven to be the key regulators of gene expression in virtually all bi ...
