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problem set
problem set

... The sequences in a pre-mRNA that dictate where splicing occurs are located at the exon/intron boundaries of the message (Fig. 8.7 below). These sequences are bound by the snRNA components of the snRNPs that make up spliceosomes (Fig. 8.9). Thus, the intron sequences ultimately tell the splicing mach ...
gene to protein 1
gene to protein 1

... 1. Base-pair substitutions involving the third base of a codon are unlikely to result in an error in the polypeptide. This is because a. substitutions are corrected before transcription begins. b. substitutions are restricted to introns. c. the base-pairing rules are less strict for the third base o ...
What is Biology? The word biology is 1………………………. from the
What is Biology? The word biology is 1………………………. from the

... biochemistry. A 9………………………. area of molecular biology concerns understanding how various cellular systems interact in terms of the way DNA, RNA and protein synthesis function, mainly dealing with the study of proteins, nucleic acids, and enzymes, and the macromolecules essential to life processes. T ...
04/03
04/03

... In all cells, constitutive expression of transcription factors that bind to upstream promoter elements ensures active transcription at all times. ...
NZY M-MuLV Reverse Transcriptase
NZY M-MuLV Reverse Transcriptase

... enzyme gives high yields of first-strand cDNA up to 7 kb. NZY M-MuLV Reverse Transcriptase can be used in firststrand cDNA synthesis experiments, RT-PCR, RT-qPCR, DNA labelling and analysis of RNA by primer extension. ...
Biology Slide 1 of 39 End Show
Biology Slide 1 of 39 End Show

... DNA is copied in the form of RNA ...
gida bi̇yoteknoloji̇si̇-2
gida bi̇yoteknoloji̇si̇-2

... • In a living cell, many metabolic reactions are carried out by proteins • Proteins are one of the most important elements of the living organisms. E.g: Blood plasma prroteins Hormons Anticores Enzymes Chloroplast Mithocondria Cell wall proteins ...
No Slide Title
No Slide Title

... Each aaRS recognizes its particular amino acid and the tRNAs coding for that amino acid. Accurate translation of the genetic code depends on attachment of each amino acid to an appropriate tRNA. Domains of tRNA recognized by an aaRS are called identity elements. Most identity elements are in the ...
Gene Regulation - Biomedical Informatics
Gene Regulation - Biomedical Informatics

... 46. Many of the eukaryotic genes contain two or more protein-coding exons and intervening non-coding introns. 47. In prokaryotic cells, translation of an mRNA into protein can begin from the 5’ end of the mRNA even while the 3’ end is still being copied from DNA. 48. In eukaryotic cells, the primary ...
Gene expression
Gene expression

... • Is the DNA in different cells of your body exactly the same? • If the DNA in every cell is the same, how do cells become different from each other? • The light switch analogy: – Cells “turn on,” or transcribe and translate only those genes that code for the proteins they need. Other genes are “tur ...
Slide 1
Slide 1

... subunit of RNA polymerase. These promoters have very similar sequences. • Eukarya: the major classes of RNA are transcribed by three different RNA polymerases, with RNA polymerase II producing most mRNA. • Archaea: have a single RNA that resembles in structure and function the RNA polymerase II. ...
Transcription PPT
Transcription PPT

Genetics/DNA PowerPoint
Genetics/DNA PowerPoint

... replication is DNA polymerase. It “proofreads” each new DNA strand to make sure that each new copy is identical to the original. ...
Reverse Genetics -
Reverse Genetics -

... •  RNAi is not heritable in a Mendelian sense. - Endogenous target gene shows no sequence change. - Can have epigenetic changes (methylation in plants). •  Does not require 1:1 stoichiometry of dsRNA to endogenous target mRNA. (Antisense RNA requires > 20 fold excess.) •  Endogenous homologous mRNA ...
Chapter 19: Viruses 1. Viral Structure & Reproduction What exactly is a Virus?
Chapter 19: Viruses 1. Viral Structure & Reproduction What exactly is a Virus?

... maintain homeostasis in any way **It’s hard to “kill” something that’s not really alive, so antibiotics that kill bacteria, fungi, etc, do NOT harm viruses** ...
Chapter 17 notes
Chapter 17 notes

... • DNA portion (one gene in length) unwinds, unzips • Free RNA nucleotides pair up on 1 of the DNA strands • Promoters signal initiation of transcription until terminator sequence is reached, then it breaks off & DNA rejoins & rewinds • Modified, then mRNA travels to cytoplasm ...
Chapter 17~ From Gene to Protein
Chapter 17~ From Gene to Protein

... RNA polymerases  3 RNA polymerase enzymes  RNA polymerase 1  only transcribes rRNA genes  makes ribosomes  RNA polymerase 2  transcribes genes into mRNA  RNA polymerase 3  only transcribes tRNA genes  each has a specific promoter sequence it recognizes ...
Proteins determine what?
Proteins determine what?

... • 1. Unwind and separation of DNA; • 2. DNA polymerase enzyme adds in freefloating nucleotides to each DNA strand; • 3. Covalent bonds are formed between sugars and phosphates and H-bonds between the nitrogen bases; • 4. resulting in 2 identical DNA helixes ...
PartThreeAnswers.doc
PartThreeAnswers.doc

... AAUAAA. After RNA polymerase II has transcribed beyond this sequence, an endonuclease (uncharacterized at this time) cleaves the primary transcript at a position about 25 to 30 nucleotides 3' to the AAUAAA. Then the enzyme polyadenylate polymerase adds a string of 20 to 250 A's to the free 3' end, g ...
Eukaryotic Gene Regulation
Eukaryotic Gene Regulation

... • Small fragments of RNA strands called Micro RNA (miRNA) (22 nucleotides in length). Can regulated gene expression in a number of ways: – Degrade the target (mature) mRNA – Prevent the early stages on translation by ribosome “drop off” – Affect chromatin Remodelling by causing histones to bind more ...
Topic 3 The chemistry of life
Topic 3 The chemistry of life

... 49. The exposed bases of each strand are then paired with an available nucleotide by complementary base pairing. The result is two strands where only one was first present. 50. DNA polymerase is an enzyme that allows the connection between nucleotides lined up by base-pairing. 51. This replication i ...
2.7 Review - Peoria Public Schools
2.7 Review - Peoria Public Schools

... 49. The exposed bases of each strand are then paired with an available nucleotide by complementary base pairing. The result is two strands where only one was first present. 50. DNA polymerase is an enzyme that allows the connection between nucleotides lined up by base-pairing. 51. This replication i ...
Worksheet 13.2
Worksheet 13.2

... (pages 366-371; iBooks pages 621-629) 1. Proteins are made by joining _____________________ into long called polypeptides. 2. How can only four bases in RNA carry instructions for 20 different amino acids? ...
Biology - Meester Martinez
Biology - Meester Martinez

... Copyright Pearson Prentice Hall ...
Removal of introns CORRECT ANSWER
Removal of introns CORRECT ANSWER

... • Which of the following is unique to prokaryotes? A. Coupled transcription-translation CORRECT ANSWER B. Removal of introns C. 3' polyadenylation D. mRNA capping E. Promoter sequences ...
< 1 ... 108 109 110 111 112 113 114 115 116 ... 225 >

RNA silencing

RNA silencing (associated with the concept of post-transcriptional gene silencing or RNA interference) refers to a family of gene silencing effects by which the expression of one or more genes is downregulated or entirely suppressed by non-coding RNAs, particularly small RNAs. It may also refer to the introduction of a synthetic antisense RNA molecule used in scientific experiments on gene expression. RNA silencing may also be defined as sequence-specific regulation of gene expression triggered by double-stranded RNA (dsRNA). RNA silencing mechanisms are highly conserved in most eukaryotes. The most common and well-studied example is RNA interference (RNAi), in which endogenously expressed microRNA (miRNA) or exogenously derived small interfering RNA (siRNA) induces the degradation of complementary messenger RNA. Other classes of small RNA have been identified, including piwi-interacting RNA (piRNA) and its subspecies repeat associated small interfering RNA (rasiRNA).
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