Analysis of DNA transcription termination sequences of gene coding
... The proteins involved in the synthesis of PHAs are encoded by the pha gene cluster containing two polymerase genes phaC1 and phaC2, a depolymerase gene phaZ and three regulatory genes phaD, phaF and phaI. Even though the key genes engaged in the process of PHAs synthesis are known, there is a lack o ...
... The proteins involved in the synthesis of PHAs are encoded by the pha gene cluster containing two polymerase genes phaC1 and phaC2, a depolymerase gene phaZ and three regulatory genes phaD, phaF and phaI. Even though the key genes engaged in the process of PHAs synthesis are known, there is a lack o ...
Localization and structural analysis of the ribosomal RNA operons of
... E. coli rRNA operons. The fact that insertion of a cartridge resulted in the loss of the wild type signal, leads to the conclusion that there are only three rRNA operons in R. sphaeroides. Thus rmA can be assigned to the 10 kb BamYU signal, rrnB to the 14 kb BamYU signal, and rmC to the 13 kb BamYU ...
... E. coli rRNA operons. The fact that insertion of a cartridge resulted in the loss of the wild type signal, leads to the conclusion that there are only three rRNA operons in R. sphaeroides. Thus rmA can be assigned to the 10 kb BamYU signal, rrnB to the 14 kb BamYU signal, and rmC to the 13 kb BamYU ...
Nonsensemediated decay of glutathione peroxidase 1 mRNA in the
... consequence of routine abnormalities in gene expression, such as aberrant or inef®cient pre-mRNA splicing and non-productive somatic cell rearrangements characteristic of the immunoglobulin and T-cell receptor genes. For reasons unknown, some mammalian mRNAs are subject to NMD exclusively in the cyt ...
... consequence of routine abnormalities in gene expression, such as aberrant or inef®cient pre-mRNA splicing and non-productive somatic cell rearrangements characteristic of the immunoglobulin and T-cell receptor genes. For reasons unknown, some mammalian mRNAs are subject to NMD exclusively in the cyt ...
Slides
... Copy Number Variation by Prof. Stephen Scherer CNVs in human genomes by Prof. Chris Ponting The Future of CNVs: Sequence base resolution and links to human disea Professor Evan Eichler – University of Washington • You will need the Stanford name and password (stanford, member) in order to watch this ...
... Copy Number Variation by Prof. Stephen Scherer CNVs in human genomes by Prof. Chris Ponting The Future of CNVs: Sequence base resolution and links to human disea Professor Evan Eichler – University of Washington • You will need the Stanford name and password (stanford, member) in order to watch this ...
PAX6 mRNA Transcript Analysis in Various Ocular/Non
... the ocular/non-ocular tissues expressed the β-actin gene equal levels and showed 540bp amplification. Majority of the genes commonly considered to have a housekeeping function (e.g., β-actin and GAPDH) exhibit considerably variable expression levels from one tissue type to another. However, expressi ...
... the ocular/non-ocular tissues expressed the β-actin gene equal levels and showed 540bp amplification. Majority of the genes commonly considered to have a housekeeping function (e.g., β-actin and GAPDH) exhibit considerably variable expression levels from one tissue type to another. However, expressi ...
IMPROVE SMALL RNA-MEDIATED GENE SILENCING
... trigger in Caenorhabditis elegans and proposed the term RNA interference (RNAi) for the first time (Fire et al. 1998). Later on, the potency of dsRNA to induce gene silencing was also demonstrated in plants (Waterhouse et al. 1998), protozoa (Ngo et al. 1998), and insects (Kennerdell and Carthew 199 ...
... trigger in Caenorhabditis elegans and proposed the term RNA interference (RNAi) for the first time (Fire et al. 1998). Later on, the potency of dsRNA to induce gene silencing was also demonstrated in plants (Waterhouse et al. 1998), protozoa (Ngo et al. 1998), and insects (Kennerdell and Carthew 199 ...
Extensive tRNA gene changes in synthetic Brassica
... A total of 1157 putative tRNA genes were predicted from B. rapa genome sequences using tRNA predicting program “tRNAscan-SE”. These genes were classified into 23 groups according to the type of tRNA isoacceptor (see Fig. 1 for detailed information). There were 67 intron-containing tRNA genes, belon ...
... A total of 1157 putative tRNA genes were predicted from B. rapa genome sequences using tRNA predicting program “tRNAscan-SE”. These genes were classified into 23 groups according to the type of tRNA isoacceptor (see Fig. 1 for detailed information). There were 67 intron-containing tRNA genes, belon ...
Increased transcription rates correlate with increased reversion rates
... would be expected to have higher rates of transcription and mutation than cells that can not activate derepressed genes (relA mutants). In addition, inactivation of a repressor would also be expected to increase transcription and thereby affect mutation rates. Unlike the situation in regulated strai ...
... would be expected to have higher rates of transcription and mutation than cells that can not activate derepressed genes (relA mutants). In addition, inactivation of a repressor would also be expected to increase transcription and thereby affect mutation rates. Unlike the situation in regulated strai ...
Morphology of nuclear transcription | SpringerLink
... unique conformation in the genome. Individual genes are arranged in a repetitive head-to-tail orientation. The transcribed genes (transcription units; TU) are separated by intergenic spacer sequences (IGS). Several tens of such repeats form a nucleolar organizer region (NOR) which, in humans, lies e ...
... unique conformation in the genome. Individual genes are arranged in a repetitive head-to-tail orientation. The transcribed genes (transcription units; TU) are separated by intergenic spacer sequences (IGS). Several tens of such repeats form a nucleolar organizer region (NOR) which, in humans, lies e ...
Is HP1 an RNA detector that functions both in repression and
... protein 1 (HP1)* by Elgin and co-workers in the mid1980s suggested that the distinct cytological features of this chromatin may be related to its unique nucleoprotein composition (James and Elgin, 1986; James et al., 1989). HP1 immunostaining on polytene chromosomes from Drosophila larval salivary g ...
... protein 1 (HP1)* by Elgin and co-workers in the mid1980s suggested that the distinct cytological features of this chromatin may be related to its unique nucleoprotein composition (James and Elgin, 1986; James et al., 1989). HP1 immunostaining on polytene chromosomes from Drosophila larval salivary g ...
Mitochondrial Transcript Processing and Restoration of Male Fertility
... of the CMS-associated mitochondrial proteins. Although Rf2 has no detectable effect on transcript processing or URF13 accumulation in maize ( Dewey et al. 1987; Wise RP, Dill CL, and Schnable PS, unpublished data), the abundance of URF13 is reduced by approximately 80% in all tissues examined in pla ...
... of the CMS-associated mitochondrial proteins. Although Rf2 has no detectable effect on transcript processing or URF13 accumulation in maize ( Dewey et al. 1987; Wise RP, Dill CL, and Schnable PS, unpublished data), the abundance of URF13 is reduced by approximately 80% in all tissues examined in pla ...
Telomere Shortening and Tumor Formation by Mouse Cells Lacking
... MEF cultures from G1 WT and mTR-/- G2, G4 and G6 animals → all chromosome ends were examined with a (TTAGGG)probe, which had a fluorescence-tag Definition: 1 telomere fluorescence unit (TFU) represents 1 kb of (TTAGGG)-repeats ...
... MEF cultures from G1 WT and mTR-/- G2, G4 and G6 animals → all chromosome ends were examined with a (TTAGGG)probe, which had a fluorescence-tag Definition: 1 telomere fluorescence unit (TFU) represents 1 kb of (TTAGGG)-repeats ...
informe tecnológico de patentes
... Huntingtin messenger RNA (mRNA) should provide therapeutic benefit, but normal Huntingtin likely contributes to neuronal function [11-13]. No si RNA strategy can yet distinguish among the normal and disease Huntingtin alleles and other mRNAs containing CAG repeats [14]. siRNAs targeting the disease ...
... Huntingtin messenger RNA (mRNA) should provide therapeutic benefit, but normal Huntingtin likely contributes to neuronal function [11-13]. No si RNA strategy can yet distinguish among the normal and disease Huntingtin alleles and other mRNAs containing CAG repeats [14]. siRNAs targeting the disease ...
Gel Electrophoresis - Integrated DNA Technologies
... The term “electrophoresis” was originally meant to refer to the migration of charged particles in an electrical field. The alternative term “ionophoresis” had been reserved for the migration of lower molecular weight substances in stabilized media such as gels and powders [1]. Today, the general ter ...
... The term “electrophoresis” was originally meant to refer to the migration of charged particles in an electrical field. The alternative term “ionophoresis” had been reserved for the migration of lower molecular weight substances in stabilized media such as gels and powders [1]. Today, the general ter ...
- Philsci-Archive
... relationship between DNA sequence and the proteins for which it “codes” is “specific”. My concern is with what this claim means—with the empirical features that biologists believe this relationship to possess which leads them to think of it as specific -- and only secondarily with the complicated an ...
... relationship between DNA sequence and the proteins for which it “codes” is “specific”. My concern is with what this claim means—with the empirical features that biologists believe this relationship to possess which leads them to think of it as specific -- and only secondarily with the complicated an ...
An rpoB signature sequence provides unique resolution for the
... was repeated until there was no further resolution in the resulting tree. These consecutive trees are called tier trees in this study. Signature sequence localization. The position of the indel within the rpoB gene was visualized using SMART (http://smart.emblheidelberg.de/). The position of the ind ...
... was repeated until there was no further resolution in the resulting tree. These consecutive trees are called tier trees in this study. Signature sequence localization. The position of the indel within the rpoB gene was visualized using SMART (http://smart.emblheidelberg.de/). The position of the ind ...
localization of histone gene transcripts in newt lampbrush
... are now pipetted on to a preparation of lampbrush chromosomes, and covered with an acidwashed 13-mm square coverslip. Three such covered preparations are placed in a square plastic dish, propped up on 2 L-shaped glass rods, with a pad of absorbent paper below liberally wetted with 20 x SSC, and the ...
... are now pipetted on to a preparation of lampbrush chromosomes, and covered with an acidwashed 13-mm square coverslip. Three such covered preparations are placed in a square plastic dish, propped up on 2 L-shaped glass rods, with a pad of absorbent paper below liberally wetted with 20 x SSC, and the ...
A rule-based kinetic model of RNA polymerase II C
... modelled as acting on Ser2P during elongation and on both Ser2P and Ser5P during recycling. The Ser2 kinase Bur1 is recruited to CTD repeats of elongating RNAPII phosphorylated on Ser5. Bur1 augments the major Ser2 kinase (Ctk1) and is capable of phosphorylating CTD repeats at Ser2, when Ctk1 is ...
... modelled as acting on Ser2P during elongation and on both Ser2P and Ser5P during recycling. The Ser2 kinase Bur1 is recruited to CTD repeats of elongating RNAPII phosphorylated on Ser5. Bur1 augments the major Ser2 kinase (Ctk1) and is capable of phosphorylating CTD repeats at Ser2, when Ctk1 is ...
Spider Silk - Consortium for Mathematics and its Applications
... Because of significant laboratory research, we already know the amino acid sequences for many silk proteins. Additionally, research suggests that the technology to manufacture spider silk is not too far off. But perhaps we could do even better. What if we changed the amino acid sequence? Could we fi ...
... Because of significant laboratory research, we already know the amino acid sequences for many silk proteins. Additionally, research suggests that the technology to manufacture spider silk is not too far off. But perhaps we could do even better. What if we changed the amino acid sequence? Could we fi ...
8 The Genetic Code
... In 1966, Francis Crick developed the wobble hypothesis, which proposed that some nonstandard pairings of bases could occur at the third position of a codon. For example, a G in the anticodon may pair with either a C or a U in the third position of the codon (Figure 8.3). The important thing to remem ...
... In 1966, Francis Crick developed the wobble hypothesis, which proposed that some nonstandard pairings of bases could occur at the third position of a codon. For example, a G in the anticodon may pair with either a C or a U in the third position of the codon (Figure 8.3). The important thing to remem ...
A defense-offense multi-layered regulatory switch in a pathogenic
... starts at the OFF state, when the level of the sRNA regulator is 0 and the TF is expressed and active (A), leading to a high level of protein 1 and a low level of protein 2 (B). Upon change in condition (ON step, at t = 10 h) the sRNA is activated, leading to a decrease in the level of the TF (A) as ...
... starts at the OFF state, when the level of the sRNA regulator is 0 and the TF is expressed and active (A), leading to a high level of protein 1 and a low level of protein 2 (B). Upon change in condition (ON step, at t = 10 h) the sRNA is activated, leading to a decrease in the level of the TF (A) as ...
The trp Operon - aandersonbiology
... and automatically used in every cell all the time. Each cell uses or expresses only a small portion of its total number of genes. The finger cells are not expressing the gene for insulin production. In 1961, Fracois Jacob and Jacques Monod described the control of gene expression as the operon model ...
... and automatically used in every cell all the time. Each cell uses or expresses only a small portion of its total number of genes. The finger cells are not expressing the gene for insulin production. In 1961, Fracois Jacob and Jacques Monod described the control of gene expression as the operon model ...
Sequence Heterogeneities Among 16s
... RNA sequences are now widely used for phylogenetic analyses and identifications of species. For phylogeny, rRNA sequences have become popular because they are universally present and have a conserved function (they are homologous in all organisms), because they are easy to sequence, and finally beca ...
... RNA sequences are now widely used for phylogenetic analyses and identifications of species. For phylogeny, rRNA sequences have become popular because they are universally present and have a conserved function (they are homologous in all organisms), because they are easy to sequence, and finally beca ...
Widespread expression of the bovine Agouti gene results from at
... identified in any studied bovine breed individuals suggesting that the Agouti coding region is highly conserved among cattle breeds. Agouti is expressed in every studied bovine tissue Tissue distribution of bovine Agouti mRNA was first examined by RT-PCR using gene-specific primers Ag1 and Ag2 (Fig. ...
... identified in any studied bovine breed individuals suggesting that the Agouti coding region is highly conserved among cattle breeds. Agouti is expressed in every studied bovine tissue Tissue distribution of bovine Agouti mRNA was first examined by RT-PCR using gene-specific primers Ag1 and Ag2 (Fig. ...
Calculating Nucleic Acid or Protein Concentration
... While direct concentration of nucleic acids is fairly accurate, there can be dramatic variation in direct protein concentration results measured at OD280. Because only tryptophan, tyrosine and cysteine contribute significantly to protein absorbance at 280 nm, the light absorption of protein is depen ...
... While direct concentration of nucleic acids is fairly accurate, there can be dramatic variation in direct protein concentration results measured at OD280. Because only tryptophan, tyrosine and cysteine contribute significantly to protein absorbance at 280 nm, the light absorption of protein is depen ...