Slide - Gerstein Lab
... information associated with these molecules, on a large-scale. • Bioinformatics is a practical discipline with many applications. ...
... information associated with these molecules, on a large-scale. • Bioinformatics is a practical discipline with many applications. ...
An accessible database for mouse and human whole transcriptome
... especially in transcriptome studies, where different primer combinations allow identification of specific transcripts such as splice variants or precursor messenger RNA. Several softwares that implement various rules for optimal primer design are available. Nevertheless, as designing qPCR primers ne ...
... especially in transcriptome studies, where different primer combinations allow identification of specific transcripts such as splice variants or precursor messenger RNA. Several softwares that implement various rules for optimal primer design are available. Nevertheless, as designing qPCR primers ne ...
Resources for the map-based cloning of tga1
... contig numbers for the B73 and Mo17 BAC libraries that were in use at the time of our research. Since these numbers change over time as the physical map for maize is improved, current contig numbers can be found by searching the maize physical map for any of the BAC clones at the Arizona Genomics In ...
... contig numbers for the B73 and Mo17 BAC libraries that were in use at the time of our research. Since these numbers change over time as the physical map for maize is improved, current contig numbers can be found by searching the maize physical map for any of the BAC clones at the Arizona Genomics In ...
An Introduction to RNA Interference (RNAi)
... biological pathway is the ability to translate knowledge on normal function into the development of even safer and more efficacious therapeutics. Indeed, due to their central importance in many cell biological processes, an unprecedented research effort is aimed at elucidating additional RNA biology ...
... biological pathway is the ability to translate knowledge on normal function into the development of even safer and more efficacious therapeutics. Indeed, due to their central importance in many cell biological processes, an unprecedented research effort is aimed at elucidating additional RNA biology ...
Kallikrein-like prorenin-converting enzymes in inbred
... Tissue preparation. Part of the surgically removed submandibular gland was frozen in liquid nitrogen for further use, whereas the rest of it was fixed in chilled phosphate buffered saline (PBS) containing 3% paraformaldehyde and 0.02% diethylpyrocarbonate for 2–3 h. Preparation of tissue extract. One ...
... Tissue preparation. Part of the surgically removed submandibular gland was frozen in liquid nitrogen for further use, whereas the rest of it was fixed in chilled phosphate buffered saline (PBS) containing 3% paraformaldehyde and 0.02% diethylpyrocarbonate for 2–3 h. Preparation of tissue extract. One ...
SMN1
... Mechanisms proposed to explain how reduced SMN levels cause SMA According to one hypothesis, reduced SMN levels result in reduced assembly of Sm proteins onto snRNA. This unevenly alters the levels of specific endogenous snRNPs, such as those used to splice minor introns (particularly U11) from pr ...
... Mechanisms proposed to explain how reduced SMN levels cause SMA According to one hypothesis, reduced SMN levels result in reduced assembly of Sm proteins onto snRNA. This unevenly alters the levels of specific endogenous snRNPs, such as those used to splice minor introns (particularly U11) from pr ...
GENE EXPRESSION
... the most part, is then translated into protein. Control mechanisms are exercised along the way. Without some control of gene expression, an Escherichia coli cell, for example, would produce all its proteins in large quantities all the time, and all the cells in a eukaryotic organism would be identic ...
... the most part, is then translated into protein. Control mechanisms are exercised along the way. Without some control of gene expression, an Escherichia coli cell, for example, would produce all its proteins in large quantities all the time, and all the cells in a eukaryotic organism would be identic ...
Q-RT-PCR replaced 4-21-06
... d. Initial evaluation should also include NRTC (no reverse transcriptase control) e. Standard curves can be generated using serial dilutions of cDNA with highest content of mRNA of interest 8. Cap tubes and place in PE 5700 QPCR machine 9. Run at 950C for 10 minutes and then 40 cycles of 950C for 15 ...
... d. Initial evaluation should also include NRTC (no reverse transcriptase control) e. Standard curves can be generated using serial dilutions of cDNA with highest content of mRNA of interest 8. Cap tubes and place in PE 5700 QPCR machine 9. Run at 950C for 10 minutes and then 40 cycles of 950C for 15 ...
Protocol for Real-Time RT-PCR - MGH-PGA
... to the appropriate templates, we obtained positive PCR results in contrast to the otherwise negative PCRs (see our paper for more details). Poor PCR amplification efficiency. The accuracy of real-time PCR is highly dependent on PCR efficiency. A reasonable efficiency should be at least 80%. Poor pri ...
... to the appropriate templates, we obtained positive PCR results in contrast to the otherwise negative PCRs (see our paper for more details). Poor PCR amplification efficiency. The accuracy of real-time PCR is highly dependent on PCR efficiency. A reasonable efficiency should be at least 80%. Poor pri ...
Identification and Analysis of Arabidopsis Expressed Sequence
... correspond to RNAs that lack significant open reading frames and encode RNA as their final product. Accumulating evidence indicates that such “non-coding RNAs” (ncRNAs) can play critical roles in a wide range of cellular processes, including chromosomal silencing, transcriptional regulation, develop ...
... correspond to RNAs that lack significant open reading frames and encode RNA as their final product. Accumulating evidence indicates that such “non-coding RNAs” (ncRNAs) can play critical roles in a wide range of cellular processes, including chromosomal silencing, transcriptional regulation, develop ...
Supplementary Information (doc 1628K)
... the RNeasy kit (Qiagen, Inc., Germantown, MD). Samples were analyzed using the Genechip primeview assay for target synthesis and labeling. Samples were hybridized to the Genechip primeview human gene expression array and those demonstrating a cutoff greater or less than 1.5-fold difference from the ...
... the RNeasy kit (Qiagen, Inc., Germantown, MD). Samples were analyzed using the Genechip primeview assay for target synthesis and labeling. Samples were hybridized to the Genechip primeview human gene expression array and those demonstrating a cutoff greater or less than 1.5-fold difference from the ...
Use of Gene-Specific Primer Cocktails for First
... chances are slim that priming will begin at the very The genes for α1-adrenoreceptors (α1ARs) con3′ end of the transcript. Lastly, a gene-specific tain a large (~16 kb) intron between the sixth and primer can be used to prime first-strand cDNA seventh transmembrane domains.3 The primers synthesis. T ...
... chances are slim that priming will begin at the very The genes for α1-adrenoreceptors (α1ARs) con3′ end of the transcript. Lastly, a gene-specific tain a large (~16 kb) intron between the sixth and primer can be used to prime first-strand cDNA seventh transmembrane domains.3 The primers synthesis. T ...
Sequence analysis of three mitochondrial DNA molecules reveals
... exonic ORFs (the eight structural genes only) is 79.6 and 77.5%, respectively. VAR1 differs from the average by having an A+T content at 94.2%, and ATP9 differs by having only 65.8% A+T. The intronic ORF encoded by S.castellii COX1.2 has an A+T content (76.9%) close to the average. For S.servazzii, ...
... exonic ORFs (the eight structural genes only) is 79.6 and 77.5%, respectively. VAR1 differs from the average by having an A+T content at 94.2%, and ATP9 differs by having only 65.8% A+T. The intronic ORF encoded by S.castellii COX1.2 has an A+T content (76.9%) close to the average. For S.servazzii, ...
In vivo evidence for the prokaryotic model of extended codon
... Figure 2B show that in the U-AUG+U strain, the mutant tRNA is fully aminoacylated. The chloroplast tRNALeu was used in all cases as a control for successful retention or removal of the charged amino acid. Finally, to determine whether the insertion of a trnfM gene downstream of petA affected petA mR ...
... Figure 2B show that in the U-AUG+U strain, the mutant tRNA is fully aminoacylated. The chloroplast tRNALeu was used in all cases as a control for successful retention or removal of the charged amino acid. Finally, to determine whether the insertion of a trnfM gene downstream of petA affected petA mR ...
Analysis and Characterization of Nucleic Acids and Proteins
... In end labeling, labeled nucleotides are added to the end of the probe using terminal transferase or T4 polynucleotide kinase. ...
... In end labeling, labeled nucleotides are added to the end of the probe using terminal transferase or T4 polynucleotide kinase. ...
Author`s personal copy - Real Jardín Botánico
... 1055-7903/$ - see front matter Ó 2011 Elsevier Inc. All rights reserved. doi:10.1016/j.ympev.2011.06.023 ...
... 1055-7903/$ - see front matter Ó 2011 Elsevier Inc. All rights reserved. doi:10.1016/j.ympev.2011.06.023 ...
A Novel Two Domain-Fusion Protein in Cyanobacteria with
... PCR (qPCR), and Northern Hybridization Whole-cell RNA was isolated, purified, and stored at –80C (Steunou et al., 2006). The RNA used for both reverse transcription PCR (RT-PCR) and quantitative PCR (qPCR) was subjected to DNAse digestion (TurboDNAse, Ambion) and then purified by phenol/chloroform ...
... PCR (qPCR), and Northern Hybridization Whole-cell RNA was isolated, purified, and stored at –80C (Steunou et al., 2006). The RNA used for both reverse transcription PCR (RT-PCR) and quantitative PCR (qPCR) was subjected to DNAse digestion (TurboDNAse, Ambion) and then purified by phenol/chloroform ...
cbb752-mg-spr09-bioinfo
... information associated with these molecules, on a large-scale. • Bioinformatics is a practical discipline with many applications. ...
... information associated with these molecules, on a large-scale. • Bioinformatics is a practical discipline with many applications. ...
Directed Evolution of Polymerases To Accept Nucleotides with
... primers are, however, tagged with DNA sequences that contain multiple P’s. The small amounts of chimeric primers are rapidly consumed in the initial rounds of PCR, requiring the external primers to later “carry” the amplification. Because the external primers must be present at high concentrations fo ...
... primers are, however, tagged with DNA sequences that contain multiple P’s. The small amounts of chimeric primers are rapidly consumed in the initial rounds of PCR, requiring the external primers to later “carry” the amplification. Because the external primers must be present at high concentrations fo ...
Alnylam Licenses Intellectual Property from Cold Spring Harbor
... has built in the fundamental patents, technology, and know-how that underlie the discovery, development and commercialization of RNAi therapeutics Under the terms of the agreement, Alnylam receives a non-exclusive license from Cold Spring Harbor Laboratory to therapeutic uses of patent applications ...
... has built in the fundamental patents, technology, and know-how that underlie the discovery, development and commercialization of RNAi therapeutics Under the terms of the agreement, Alnylam receives a non-exclusive license from Cold Spring Harbor Laboratory to therapeutic uses of patent applications ...
Complete Thyroxine-Binding Globulin (TBG) Deficiency Produced
... creates a new acceptor splice site one nucleotide upstream of the authentic acceptor splice site adding an intronic G to the downstream exon. This is in agreement with the majority of acceptor splice site mutations reported (15, 18). Exonic and intronic recognition sequences have an established role ...
... creates a new acceptor splice site one nucleotide upstream of the authentic acceptor splice site adding an intronic G to the downstream exon. This is in agreement with the majority of acceptor splice site mutations reported (15, 18). Exonic and intronic recognition sequences have an established role ...
Microbial Genetics - MyCourses
... Learning Objectives 8-1 Define genetics, genome, chromosome, gene, genetic code, genotype, phenotype, and genomics. 8-2 Describe how DNA serves as genetic information. 8-3 Describe the process of DNA replication. 8-4 Describe protein synthesis, including transcription, RNA processing, and translatio ...
... Learning Objectives 8-1 Define genetics, genome, chromosome, gene, genetic code, genotype, phenotype, and genomics. 8-2 Describe how DNA serves as genetic information. 8-3 Describe the process of DNA replication. 8-4 Describe protein synthesis, including transcription, RNA processing, and translatio ...
A pseudogene cluster in the leader region of the Euglena
... 16S rRNA leader region 1s a r e l i c of 16S-23S rRNA gene cluster having no functions for the genes. By what reasons the pseudogene cluster emerged? A large rRNA precursor containing both the 16S and 23S rRNA genes was observed in spinach (27,28) and Chlamidomonas (29) chloroplasts. A large rRNA of ...
... 16S rRNA leader region 1s a r e l i c of 16S-23S rRNA gene cluster having no functions for the genes. By what reasons the pseudogene cluster emerged? A large rRNA precursor containing both the 16S and 23S rRNA genes was observed in spinach (27,28) and Chlamidomonas (29) chloroplasts. A large rRNA of ...
Ribosomal RNA Secondary Structure
... pairing and nonpairing bases are 1,082 (54.4%) and 907 (45.6%)) respectively (table 1). These proportions are not significantly different [x2 = 0.03, degrees of freedom (df) = 1, P > 0.81 from the proportions predicted by the Xenopus model of secondary structure for the entire 28s gene [ 2,244 (54.6 ...
... pairing and nonpairing bases are 1,082 (54.4%) and 907 (45.6%)) respectively (table 1). These proportions are not significantly different [x2 = 0.03, degrees of freedom (df) = 1, P > 0.81 from the proportions predicted by the Xenopus model of secondary structure for the entire 28s gene [ 2,244 (54.6 ...
Applications of Bioinformatics and Genomics/Proteomics
... Office hours: Every Wednesday and Friday from 9 AM to 10 AM in the office of Dr. Fedorov (Room 308 at Health Science building, HSC; tel: 419-383-5270). Also, students may contact remotely via Skype (Afedorov_lab) every Wednesday and Friday from 9 am to 10 am. Homework time policy: Each homework assi ...
... Office hours: Every Wednesday and Friday from 9 AM to 10 AM in the office of Dr. Fedorov (Room 308 at Health Science building, HSC; tel: 419-383-5270). Also, students may contact remotely via Skype (Afedorov_lab) every Wednesday and Friday from 9 am to 10 am. Homework time policy: Each homework assi ...