Translational control of regA, a key gene controlling
... have a natural PmeI cloning site at the 5⬘ end and a BglII cloning site at the 3⬘ end with which they could be inserted in the pVcRegA1 plasmid. A DNA fragment consisting of five copies of a BamHI linker sequence was used previously to generate a stable hairpin that blocked ribosomal scanning but no ...
... have a natural PmeI cloning site at the 5⬘ end and a BglII cloning site at the 3⬘ end with which they could be inserted in the pVcRegA1 plasmid. A DNA fragment consisting of five copies of a BamHI linker sequence was used previously to generate a stable hairpin that blocked ribosomal scanning but no ...
Mechanisms of Transcription-Replication
... plasmid carrying the lacIq gene (Fig. 4C), it was completely repressed in the absence of IPTG, while being strongly activated in its presence (20). Figure 4C shows that replication stalling in this case was evident only when transcription from the head-on–oriented trc promoter was active. We have al ...
... plasmid carrying the lacIq gene (Fig. 4C), it was completely repressed in the absence of IPTG, while being strongly activated in its presence (20). Figure 4C shows that replication stalling in this case was evident only when transcription from the head-on–oriented trc promoter was active. We have al ...
Multistep process of FUS aggregation in the cell cytoplasm involves
... evolutionary distant protein, yeast Npl3 (Fig. 3A). FUS-TDP-43 chimeric protein readily aggregated in the cytoplasm, appeared in detergent-insoluble fraction (Fig. 3D) and formed structures resembling aggregates in cells expressing FUS R522G (Fig. 3B). In contrast, FUS-Npl3 chimeric protein was foun ...
... evolutionary distant protein, yeast Npl3 (Fig. 3A). FUS-TDP-43 chimeric protein readily aggregated in the cytoplasm, appeared in detergent-insoluble fraction (Fig. 3D) and formed structures resembling aggregates in cells expressing FUS R522G (Fig. 3B). In contrast, FUS-Npl3 chimeric protein was foun ...
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... INTRODUCTION The H19 gene was cloned 20 years ago (Pachnis et al., 1988) and was one of the first imprinted genes to be identified. Genomic imprinting is an epigenetic mechanism that leads to parent-of-origin specific monoallelic expression. Thus, H19 is expressed from the maternal allele in mouse a ...
... INTRODUCTION The H19 gene was cloned 20 years ago (Pachnis et al., 1988) and was one of the first imprinted genes to be identified. Genomic imprinting is an epigenetic mechanism that leads to parent-of-origin specific monoallelic expression. Thus, H19 is expressed from the maternal allele in mouse a ...
Transvection and pairing of a Drosophila Hox long noncoding RNA
... segments (Pattatucci et al. 1991). Strikingly, five different GOF mutations associated with translocations or inversions with breakpoints mapping to the fushi tarazu– Antennapedia (ftz-Antp) interval result in mis-regulation of Scr in trans, on the wildtype (wt) chromosome (Southworth and Kennison 2 ...
... segments (Pattatucci et al. 1991). Strikingly, five different GOF mutations associated with translocations or inversions with breakpoints mapping to the fushi tarazu– Antennapedia (ftz-Antp) interval result in mis-regulation of Scr in trans, on the wildtype (wt) chromosome (Southworth and Kennison 2 ...
Control of ribosome traffic by position-dependent
... to be 2/sec [11] and not rate-limiting. We study various values of the on-rate Ks. The on-rate Ks for lacZ for cells growing in a minimal media has been estimated to be about 0.9/sec [11]. Note that Ks is larger than the actual initiation rate, since the occlusion time of the start codon is finite. ...
... to be 2/sec [11] and not rate-limiting. We study various values of the on-rate Ks. The on-rate Ks for lacZ for cells growing in a minimal media has been estimated to be about 0.9/sec [11]. Note that Ks is larger than the actual initiation rate, since the occlusion time of the start codon is finite. ...
Modified uridine at wobble position in tRNA of
... polymerase III (Sprague, 1995). The precursor tRNAs undergo a series of posttranscriptional processing events to generate a mature form. These events include (1) cleavage of the 5' leader by Ribonuclease P (RNase P) (Darr et al., 1992; Altman et al, 1995); (2) removal of the 3' trailer sequence by e ...
... polymerase III (Sprague, 1995). The precursor tRNAs undergo a series of posttranscriptional processing events to generate a mature form. These events include (1) cleavage of the 5' leader by Ribonuclease P (RNase P) (Darr et al., 1992; Altman et al, 1995); (2) removal of the 3' trailer sequence by e ...
Pleiotropic effects of the mouse lethal yellow (Ay) mutation
... To determine whether inhibition of Merc gene expression affected development of non-mutant embryos, a sense or antisense oligodeoxynucleotide that spanned the predicted Merc translational initiation site was injected into fertilized 1-cell embryos obtained from superovulated FVB/N or C57BL/6J mice. ...
... To determine whether inhibition of Merc gene expression affected development of non-mutant embryos, a sense or antisense oligodeoxynucleotide that spanned the predicted Merc translational initiation site was injected into fertilized 1-cell embryos obtained from superovulated FVB/N or C57BL/6J mice. ...
Molecular Biology and Applied Genetics
... approach, in which the gene products themselves are purified and their activities studied in vitro. Genetics tells that a gene product has a role in the process that are studying in vivo, but it doesn’t necessarily tell how direct that role is. Biochemistry, by contrast, tells what a factor can do i ...
... approach, in which the gene products themselves are purified and their activities studied in vitro. Genetics tells that a gene product has a role in the process that are studying in vivo, but it doesn’t necessarily tell how direct that role is. Biochemistry, by contrast, tells what a factor can do i ...
Mutations at the Darkener of apricot Locus Modulate Transcript
... 1993; ZACHAR, CHoU and BINGHAM1987). In general, the products of second-site modifier loci play a role in the expression of the mutation-causing transposable element, and in modifying its activity, result in an alteration of the mutant phenotype. We are seeking to understand the functions these modi ...
... 1993; ZACHAR, CHoU and BINGHAM1987). In general, the products of second-site modifier loci play a role in the expression of the mutation-causing transposable element, and in modifying its activity, result in an alteration of the mutant phenotype. We are seeking to understand the functions these modi ...
A set reduction and pattern matching problem motivated by Allele
... located at a single chromosomal location (a locus) [14].1 Additional genomic diversity is introduced when genes duplicated during evolution acquire a new function. Gene duplication is common in higher organisms and whole genome duplication (polyploidy) is a phenomenon that is particularly common in ...
... located at a single chromosomal location (a locus) [14].1 Additional genomic diversity is introduced when genes duplicated during evolution acquire a new function. Gene duplication is common in higher organisms and whole genome duplication (polyploidy) is a phenomenon that is particularly common in ...
Engineered bacteriophage-defence systems in bioprocessing
... genetic content and genomic organization of these seven phages exhibit significant similarities to other members of the λ-superfamily of phages23. Bioinformatic analyses have provided significant insight into the evolution of S. thermophilus phages, revealing that their genomes are molecular mosaics ...
... genetic content and genomic organization of these seven phages exhibit significant similarities to other members of the λ-superfamily of phages23. Bioinformatic analyses have provided significant insight into the evolution of S. thermophilus phages, revealing that their genomes are molecular mosaics ...
Mechanisms of translational regulation in bacteria
... gene order within an operon on the fitness of bacterial cells. Operons group functionally related genes which are together transcribed as single mRNAs in E. coli and other bacteria. Correlation of protein levels is thus to a large extent attributed to this coupling on the transcriptional level. In a ...
... gene order within an operon on the fitness of bacterial cells. Operons group functionally related genes which are together transcribed as single mRNAs in E. coli and other bacteria. Correlation of protein levels is thus to a large extent attributed to this coupling on the transcriptional level. In a ...
genomebiology.com
... remain as such due to the intron definition mechanism by which they are recognized [18-21]. Thus, there is presumably a selection against TE insertion into such introns. However, with the presumed transition from intron to exon definition during evolution [20,22], introns were freed from length cons ...
... remain as such due to the intron definition mechanism by which they are recognized [18-21]. Thus, there is presumably a selection against TE insertion into such introns. However, with the presumed transition from intron to exon definition during evolution [20,22], introns were freed from length cons ...
Natural rules for Arabidopsis thaliana
... nucleotides up- and down-stream of each splicing site were defined as “full sequence” and grouped by their locations for analysis. For sequences shorter than 50 nucleotides - “shorter sequence” on one side of splicing site, manual sequence trimming on the other side was performed to bring equal leng ...
... nucleotides up- and down-stream of each splicing site were defined as “full sequence” and grouped by their locations for analysis. For sequences shorter than 50 nucleotides - “shorter sequence” on one side of splicing site, manual sequence trimming on the other side was performed to bring equal leng ...
Identification of novel endogenous antisense transcripts by DNA
... Results: First, we designed microarray probes to target the complementary strand of genes for which an antisense counterpart had been identified only in human public cDNA sources, but not in the mouse. We observed a prominent expression signal from 66.1% of 635 target genes, and 58 genes of these sh ...
... Results: First, we designed microarray probes to target the complementary strand of genes for which an antisense counterpart had been identified only in human public cDNA sources, but not in the mouse. We observed a prominent expression signal from 66.1% of 635 target genes, and 58 genes of these sh ...
Complete Characterization of the 3 Mouse Hereditary Hemochromatosis HFE Gene and
... Mayka Sánchez,1,2 Miquel Bruguera,3 Joan Rodés,3 and Rafael Oliva1,2 ABSTRACT: The human HFE gene was identified in 1996 as the gene whose mutations are responsible for hereditary hemochromatosis in most patients. Expression analysis by Northern blot indicated that the gene was approximately 4.1 k ...
... Mayka Sánchez,1,2 Miquel Bruguera,3 Joan Rodés,3 and Rafael Oliva1,2 ABSTRACT: The human HFE gene was identified in 1996 as the gene whose mutations are responsible for hereditary hemochromatosis in most patients. Expression analysis by Northern blot indicated that the gene was approximately 4.1 k ...
The murine homologue of HIRA, a DiGeorge
... various laboratories. These attempts led to the isolation of DGCR3 and DGCR5. DGCR3 is an open reading frame (ORF) of 260 amino acids (aa) that is interrupted by the translocation breakpoint (21). However, no cDNAs were isolated and expression studies were only informative under low stringency condi ...
... various laboratories. These attempts led to the isolation of DGCR3 and DGCR5. DGCR3 is an open reading frame (ORF) of 260 amino acids (aa) that is interrupted by the translocation breakpoint (21). However, no cDNAs were isolated and expression studies were only informative under low stringency condi ...
T-box-mediated control of the anabolic proline biosynthetic genes of
... Bacillus subtilis possesses interlinked routes for the synthesis of proline. The ProJ–ProA–ProH route is responsible for the production of proline as an osmoprotectant, and the ProB–ProA–ProI route provides proline for protein synthesis. We show here that the transcription of the anabolic proBA and ...
... Bacillus subtilis possesses interlinked routes for the synthesis of proline. The ProJ–ProA–ProH route is responsible for the production of proline as an osmoprotectant, and the ProB–ProA–ProI route provides proline for protein synthesis. We show here that the transcription of the anabolic proBA and ...
pyrimidine
... The H-bonds stretch and the torsions allow some rotations, so the ropes can form roughly spherical shapes when not constrained by histones Shape is sequence-dependent, which influences protein-DNA interactions ...
... The H-bonds stretch and the torsions allow some rotations, so the ropes can form roughly spherical shapes when not constrained by histones Shape is sequence-dependent, which influences protein-DNA interactions ...
Cold-induced silencing by long antisense transcripts of an
... is mostly unknown1. We are investigating the link between noncoding RNA and chromatin regulation through analysis of FLC — a regulator of flowering time in Arabidopsis and a target of several chromatin pathways. Here we use an unbiased strategy to characterize non-coding transcripts of FLC and show ...
... is mostly unknown1. We are investigating the link between noncoding RNA and chromatin regulation through analysis of FLC — a regulator of flowering time in Arabidopsis and a target of several chromatin pathways. Here we use an unbiased strategy to characterize non-coding transcripts of FLC and show ...
Computational Definition of
... exonic splicing mutations showed good agreement between the splicing phenotype and the effect of the mutation on the motifs defined here. The large number of effective sequences implied by these results suggest that sequences that influence splicing may be very abundant in pre-mRNA. A fundamental st ...
... exonic splicing mutations showed good agreement between the splicing phenotype and the effect of the mutation on the motifs defined here. The large number of effective sequences implied by these results suggest that sequences that influence splicing may be very abundant in pre-mRNA. A fundamental st ...
The arbitrariness of the genetic code Author
... than they actually are. Of course, this does not say much about the sense in which they could be different. A more substantial claim is, for example, that the genetic code could be different because an early version of the code became established by chance events rather than by selection or stereoch ...
... than they actually are. Of course, this does not say much about the sense in which they could be different. A more substantial claim is, for example, that the genetic code could be different because an early version of the code became established by chance events rather than by selection or stereoch ...