Detailed Methods: Supplementary Information
... then centrifuged at 13,000 x g for 15 min at 4°C. The upper aqueous phase was transferred to a new eppendorf and one volume of 70% ethanol was added, then mixed thoroughly. 700μl of sample was transferred to a RNeasy Mini spin column (Qiagen) in a 2ml collection tube. The column/tube assembly was ce ...
... then centrifuged at 13,000 x g for 15 min at 4°C. The upper aqueous phase was transferred to a new eppendorf and one volume of 70% ethanol was added, then mixed thoroughly. 700μl of sample was transferred to a RNeasy Mini spin column (Qiagen) in a 2ml collection tube. The column/tube assembly was ce ...
Health and Technology
... probability that the alleles will be separated during meiosis. This means that more of the parental genotypes appear in the F2 generation. Different linked genes are inherited as a group rather than as separate units. The closer 2 genes are on a chromosome, the more likely it is that they will be in ...
... probability that the alleles will be separated during meiosis. This means that more of the parental genotypes appear in the F2 generation. Different linked genes are inherited as a group rather than as separate units. The closer 2 genes are on a chromosome, the more likely it is that they will be in ...
An Exceptional Gene: Evolution of the TSPY Gene Family
... From these two analyses, we can see that the human minor array is distinct from all the other arrays because most of its flanking regions do not align with them (Figure 1), and even where there is good alignment, it is the most divergent sequence (Figure 2). In contrast, the human major array and it ...
... From these two analyses, we can see that the human minor array is distinct from all the other arrays because most of its flanking regions do not align with them (Figure 1), and even where there is good alignment, it is the most divergent sequence (Figure 2). In contrast, the human major array and it ...
PowerPoint - Oregon State University
... • Utilize real-time Polymerase Chain Reaction (realtime PCR) to quantify the expression of three zinc transporter genes in the dorsal and ventral grey matter of the spinal cord: i) ZnT-1 ii) ZnT-3 iii) ZnT-4 ...
... • Utilize real-time Polymerase Chain Reaction (realtime PCR) to quantify the expression of three zinc transporter genes in the dorsal and ventral grey matter of the spinal cord: i) ZnT-1 ii) ZnT-3 iii) ZnT-4 ...
Introduction to Microarray Analysis (Section D1)
... Small, solid supports onto which the sequences from thousands of different genes are immobilized, or attached, at fixed locations. The DNA is printed, spotted, or actually synthesized directly onto the support. The spots themselves can be DNA, complementary DNA (cDNA, DNA synthesized from a mRNA tem ...
... Small, solid supports onto which the sequences from thousands of different genes are immobilized, or attached, at fixed locations. The DNA is printed, spotted, or actually synthesized directly onto the support. The spots themselves can be DNA, complementary DNA (cDNA, DNA synthesized from a mRNA tem ...
A Hybrid Knowledge-Driver Approach to Clustering Gene
... (SOM) [9] and hierarchical clustering [4]. Despite the fact that they have been developed outside the biological community, their application on gene expression data may yield biologically meaningful results. However, very often these algorithms are associated with one or more user-defined parameter ...
... (SOM) [9] and hierarchical clustering [4]. Despite the fact that they have been developed outside the biological community, their application on gene expression data may yield biologically meaningful results. However, very often these algorithms are associated with one or more user-defined parameter ...
Transcription factories are nuclear subcompartments that remain in
... (P < 0.001). However, a significant disruption in the interactions of Hbb-b1 with HS2 and HS3 of the LCR was observed in heat-shock treatment compared to both untreated (P = 0.014, P = 0.033) and DRB-treated (P = 0.002, P = 0.004) cells, suggesting there may be increased mobility in this region unde ...
... (P < 0.001). However, a significant disruption in the interactions of Hbb-b1 with HS2 and HS3 of the LCR was observed in heat-shock treatment compared to both untreated (P = 0.014, P = 0.033) and DRB-treated (P = 0.002, P = 0.004) cells, suggesting there may be increased mobility in this region unde ...
Human fetal normal cDNA panel
... Intended use of the products: All products sold by AMSBIO are intended for research use only and must not be used for medical diagnostic or drug purposes. AMSBIO products may not resell, modified for resale or used to manufacture commercial products without written approval from AMSBIO. Limited prod ...
... Intended use of the products: All products sold by AMSBIO are intended for research use only and must not be used for medical diagnostic or drug purposes. AMSBIO products may not resell, modified for resale or used to manufacture commercial products without written approval from AMSBIO. Limited prod ...
Comparative Genomic Study of upstream Open Reading Frames
... regulate the amount of protein synthesised from a specific mRNA. In a fraction of mRNAs, upstream open reading frames (uORFs) are present. A general method for detecting uORFs with a regulatory role is of great importance. The ribosome can recognise the AUG of a uORF as an initiation codon, translat ...
... regulate the amount of protein synthesised from a specific mRNA. In a fraction of mRNAs, upstream open reading frames (uORFs) are present. A general method for detecting uORFs with a regulatory role is of great importance. The ribosome can recognise the AUG of a uORF as an initiation codon, translat ...
Gene Section IGK@ (Immunoglobulin Kappa) Atlas of Genetics and Cytogenetics
... the lymph nodes, which will considerably increase their diversity. These somatic mutations can be analysed using IMGT/V-QUEST tool. ...
... the lymph nodes, which will considerably increase their diversity. These somatic mutations can be analysed using IMGT/V-QUEST tool. ...
PDF - 279 KB - University of Guelph
... set of downstream genes in ways that alter the phenotype. A second possibility is that the protein has changed in “specificity” but not in “function as related to the phenotype.” For example, a pair of interacting proteins in one species may have coevolved on a particular path, whereas in a second s ...
... set of downstream genes in ways that alter the phenotype. A second possibility is that the protein has changed in “specificity” but not in “function as related to the phenotype.” For example, a pair of interacting proteins in one species may have coevolved on a particular path, whereas in a second s ...
Relative expression of wild-type and activated Ki
... simplicity of this relationship to the fact that no correction is required to match the efficiencies of synthesis of the mutant and wild-type products: during both reverse transcription and the polymerase chain reaction, synthesis of both proceeds within the same reaction vessel from the same primer ...
... simplicity of this relationship to the fact that no correction is required to match the efficiencies of synthesis of the mutant and wild-type products: during both reverse transcription and the polymerase chain reaction, synthesis of both proceeds within the same reaction vessel from the same primer ...
1 4 SEX CHROMOSOMES AND SEX DETERMINATION 4.1 Sex
... Although 95% of the Y chromosome lies between the pseudoautosomal regions, fewer than 80 genes have been found here. Some of these encode proteins used by all cells (and both sexes). The others encode proteins that appear to function only in the testes. A key player in this latter group is SRY. ...
... Although 95% of the Y chromosome lies between the pseudoautosomal regions, fewer than 80 genes have been found here. Some of these encode proteins used by all cells (and both sexes). The others encode proteins that appear to function only in the testes. A key player in this latter group is SRY. ...
Return to the RNAi world: rethinking gene expression and
... These products, in turn, can direct the subsequent development of these cells such that, once differentiated in this way, these cells remain committed to their specific tasks in the animal through numerous rounds of cell division. These remarkably stable differentiation events can be maintained for ...
... These products, in turn, can direct the subsequent development of these cells such that, once differentiated in this way, these cells remain committed to their specific tasks in the animal through numerous rounds of cell division. These remarkably stable differentiation events can be maintained for ...
splicing
... unedited region at the 3’-border of editing in the pre-mRNA • The 5’-ends of the rest of the gRNAs hybridize to edited regions progressively closer to the 5’-end of the region to be edited in the pre-mRNA • All of these gRNAs provide A’s and G’s as templates for the incorporation of U’s missing from ...
... unedited region at the 3’-border of editing in the pre-mRNA • The 5’-ends of the rest of the gRNAs hybridize to edited regions progressively closer to the 5’-end of the region to be edited in the pre-mRNA • All of these gRNAs provide A’s and G’s as templates for the incorporation of U’s missing from ...
Biotechnology Laboratory
... genes of interest to map transcription start sites and to define operons. In array design 2, most genes in Synechococcus are covered with 3 probes repeated three times on each array, but highdensity UTR probes are included for all of the predicted genes. Example 1 (Electron transfer mutants): RNAs m ...
... genes of interest to map transcription start sites and to define operons. In array design 2, most genes in Synechococcus are covered with 3 probes repeated three times on each array, but highdensity UTR probes are included for all of the predicted genes. Example 1 (Electron transfer mutants): RNAs m ...
Patterns of Inheritance Worksheet #5
... acids. In many cases, the dominant allele codes for a polypeptide that works, whereas the recessive allele codes for a polypeptide that does not work. For example, suppose that the allele B codes for an enzyme that makes a black pigment in a mouse’s fur and allele b codes for a defective enzyme that ...
... acids. In many cases, the dominant allele codes for a polypeptide that works, whereas the recessive allele codes for a polypeptide that does not work. For example, suppose that the allele B codes for an enzyme that makes a black pigment in a mouse’s fur and allele b codes for a defective enzyme that ...
Diplosporous development in Boehmeria tricuspis: Insights
... Most apomicts are polyploid, and apomixis has previously been proposed to be a consequence of hybridisation and/or genome doubling, i.e. the events of polyploidisation4. However, polyploidisation alone is not sufficient to induce apomixis, as not all polyploids are apomicts6. In fact, through dosage ...
... Most apomicts are polyploid, and apomixis has previously been proposed to be a consequence of hybridisation and/or genome doubling, i.e. the events of polyploidisation4. However, polyploidisation alone is not sufficient to induce apomixis, as not all polyploids are apomicts6. In fact, through dosage ...
Pre-natal Orofacial Development - Causes of Cleft Lip and Cleft Palate
... bone (bone of roof of the mouth) that forms the palatal shelves do not come together and fuse. This disruption of the palatal shelving could also cause the soft palate of the mesenchymal cells to not fuse as well. Palatogenesis is usually complete by week twelve. Some classes of genes involved in cr ...
... bone (bone of roof of the mouth) that forms the palatal shelves do not come together and fuse. This disruption of the palatal shelving could also cause the soft palate of the mesenchymal cells to not fuse as well. Palatogenesis is usually complete by week twelve. Some classes of genes involved in cr ...
Transcription-Dependent Somatic Hypermutation Occurs
... V segments and incomplete DJ rearrangements are transcribed at low levels in immature B cells, V to DJ approximation boosts transcription by positioning one VH promoter under the control of the E enhancer (reviewed in Ref. 21). Regarding allelic exclusion, the relative transcription rate of Ig loci ...
... V segments and incomplete DJ rearrangements are transcribed at low levels in immature B cells, V to DJ approximation boosts transcription by positioning one VH promoter under the control of the E enhancer (reviewed in Ref. 21). Regarding allelic exclusion, the relative transcription rate of Ig loci ...
The Pax and large Maf families of genes in mammalian eye development Vertebrate eye development is dependent on the coordinated action of thousands of genes. A specific group of over one hundred of regulatory genes is both responsible for ocular cell
... in which a certain group of its specific target genes do not respond properly to the protein levels that are below a threshold value (Cvekl and Tamm, 2004). Haploinsufficiency of the mouse Pax2 gene results in kidney and retinal defects. Similar human PAX2 mutations cause renal‐coloboma syndrome ...
... in which a certain group of its specific target genes do not respond properly to the protein levels that are below a threshold value (Cvekl and Tamm, 2004). Haploinsufficiency of the mouse Pax2 gene results in kidney and retinal defects. Similar human PAX2 mutations cause renal‐coloboma syndrome ...