J Biol Chem, v 275, pp 12237-12242
... channels: they dramatically increase ␣1 expression at the plasma membrane, and they alter the biophysical properties of the channel currents. In general,  subunits have little effect on the expression of low voltage-activated currents (4). Although only one ␥ and ␣2␦ subunit have been characterized ...
... channels: they dramatically increase ␣1 expression at the plasma membrane, and they alter the biophysical properties of the channel currents. In general,  subunits have little effect on the expression of low voltage-activated currents (4). Although only one ␥ and ␣2␦ subunit have been characterized ...
Pax1/Pax9-Related Genes in an Agnathan Vertebrate, Lampetra
... 1% SDS, 50 g/ml yeast tRNA, 50 g/ml heparin sulfate, 0.1% Chaps, 5 mM EDTA, 1% blocking solution (Boehringer Mannheim)]. Hybridization was performed in freshly prepared hybridization buffer containing 2.0 g/ml probe for overnight (O/N) at 70°C. After hybridization, the specimens were washed in so ...
... 1% SDS, 50 g/ml yeast tRNA, 50 g/ml heparin sulfate, 0.1% Chaps, 5 mM EDTA, 1% blocking solution (Boehringer Mannheim)]. Hybridization was performed in freshly prepared hybridization buffer containing 2.0 g/ml probe for overnight (O/N) at 70°C. After hybridization, the specimens were washed in so ...
The Chromosomal Basis of Inheritance
... 1:1:1:1 RATIO However, the genes are not completely linked… in other words, since some recombinants exist… the chromosomes must have recombined via crossing over… ...
... 1:1:1:1 RATIO However, the genes are not completely linked… in other words, since some recombinants exist… the chromosomes must have recombined via crossing over… ...
MADS Monsters: Controlling Floral Organ Identity
... 1894), coined the term “homeosis” to describe variations in form that resulted in the abnormal patterning or positioning of normal body parts or organs—for example, “modification of the antenna of an insect into a foot, of the eye of a Crustacean into an antenna, of a petal into a stamen, and the li ...
... 1894), coined the term “homeosis” to describe variations in form that resulted in the abnormal patterning or positioning of normal body parts or organs—for example, “modification of the antenna of an insect into a foot, of the eye of a Crustacean into an antenna, of a petal into a stamen, and the li ...
PDF - Hopkins Lab
... Levin demonstrated that the shift from light-blue to dark-red flowers in P. drummondii decreases interspecific hybridization by 66%, indicating that the change in flower colour substantially increases prezygotic reproductive isolation12. Given the above estimates, the hybridization rate before the e ...
... Levin demonstrated that the shift from light-blue to dark-red flowers in P. drummondii decreases interspecific hybridization by 66%, indicating that the change in flower colour substantially increases prezygotic reproductive isolation12. Given the above estimates, the hybridization rate before the e ...
PCR settings, pitfalls and artefacts
... • Never opening more than one tube at a time • Using a separate thermocycler ...
... • Never opening more than one tube at a time • Using a separate thermocycler ...
WJG-23-1787
... L02/HBx cells (Figure 1A). The 20 top-ranking differentially expressed genes were subjected to a cluster analysis (Figure 1B), which determined several groups of genes with identical expression patterns. According to comparison with the control L02/pcDNA cells, the expression of GPR6, KLHL31, FOXC1, ...
... L02/HBx cells (Figure 1A). The 20 top-ranking differentially expressed genes were subjected to a cluster analysis (Figure 1B), which determined several groups of genes with identical expression patterns. According to comparison with the control L02/pcDNA cells, the expression of GPR6, KLHL31, FOXC1, ...
Smchd1 regulates a subset of autosomal genes subject to
... [9-11], and (d) the apparently random widespread monoallelic expression of possibly hundreds of individual genes spread throughout the genome [12,13]. While the molecular mechanisms underlying the different forms of monoallelic expression have unique characteristics, they may be expected to share co ...
... [9-11], and (d) the apparently random widespread monoallelic expression of possibly hundreds of individual genes spread throughout the genome [12,13]. While the molecular mechanisms underlying the different forms of monoallelic expression have unique characteristics, they may be expected to share co ...
Nat. Struct. Biol. 8, 192-194.
... fixed substrate, perhaps the nuclear matrix (see below). A way of explaining both the effect of insulators on enhancer–promoter interactions and their ability to buffer transgenes from position effects is to assume that the barrier is a consequence of the involvement of insulators in the establishme ...
... fixed substrate, perhaps the nuclear matrix (see below). A way of explaining both the effect of insulators on enhancer–promoter interactions and their ability to buffer transgenes from position effects is to assume that the barrier is a consequence of the involvement of insulators in the establishme ...
No Slide Title
... partially or wholly compensates for telomere shortening. Telomerases in all eukaryotic species share at least two components essential for catalytic activity: a telomerase reverse transcriptase protein (TERT) and a telomerase RNA. The TERT encoding gene is a retroelement. In humans, this retroelemen ...
... partially or wholly compensates for telomere shortening. Telomerases in all eukaryotic species share at least two components essential for catalytic activity: a telomerase reverse transcriptase protein (TERT) and a telomerase RNA. The TERT encoding gene is a retroelement. In humans, this retroelemen ...
RNA-Seq analysis
... Catalogue all species of transcript including: mRNAs, non-coding RNAs and small RNAs Determine the transcriptional structure of genes in terms of: Start sites 5′ and 3′ ends Splicing patterns Other post-transcriptional modifications Quantification of expression levels and comparison (d ...
... Catalogue all species of transcript including: mRNAs, non-coding RNAs and small RNAs Determine the transcriptional structure of genes in terms of: Start sites 5′ and 3′ ends Splicing patterns Other post-transcriptional modifications Quantification of expression levels and comparison (d ...
pdf
... broad-host-range plasmid pVLT33 (Fig. 1), a RSF1010 derivative and therefore able to replicate in a wide variety of Gram-negative bacteria and susceptible of efficient mobilization by the RP4 conjugation system [3]. The resultant plasmid, pSJ33, has five unique restriction sites which facilitate the ...
... broad-host-range plasmid pVLT33 (Fig. 1), a RSF1010 derivative and therefore able to replicate in a wide variety of Gram-negative bacteria and susceptible of efficient mobilization by the RP4 conjugation system [3]. The resultant plasmid, pSJ33, has five unique restriction sites which facilitate the ...
letter Widespread aneuploidy revealed by DNA microarray expression profiling
... sion bias in a 56-ORF region on rps24a∆/rps24a∆ (sector 4) cells or their isogenic parental wild-type cells (sectors 1 and 3) were streaked onto solid the right arm of chromosome medium and incubated at 30 °C for 2–4 d. Fast-growing colonies, shown to harbour an extra copy of chromosome IX, are high ...
... sion bias in a 56-ORF region on rps24a∆/rps24a∆ (sector 4) cells or their isogenic parental wild-type cells (sectors 1 and 3) were streaked onto solid the right arm of chromosome medium and incubated at 30 °C for 2–4 d. Fast-growing colonies, shown to harbour an extra copy of chromosome IX, are high ...
Working with ribonucleic acid (RNA)-based biotechnologies)
... molecules, and represent an interesting alternative to the RNAi. Unfortunately ribozyme design has proved difficult. It is likely that further RNA molecules will be identified. ...
... molecules, and represent an interesting alternative to the RNAi. Unfortunately ribozyme design has proved difficult. It is likely that further RNA molecules will be identified. ...
Recurrent Tandem Gene Duplication Gave Rise
... It is well recognized that gene duplication is prevalent in eukaryotes. Genomic analyses of model organisms have shown that over one-third of all protein-coding genes belong to multigene families (Rubin et al. 2000; Kent et al. 2003). The mechanisms of gene duplication can be classified based on its ...
... It is well recognized that gene duplication is prevalent in eukaryotes. Genomic analyses of model organisms have shown that over one-third of all protein-coding genes belong to multigene families (Rubin et al. 2000; Kent et al. 2003). The mechanisms of gene duplication can be classified based on its ...
BMC Genomics - Gene Quantification
... compared these with experimental microarray data generated from single embryonic neural stem cells in vivo. We show that the actual distribution of measured gene expression ratios for pairs of neural stem cells is much broader than that predicted from our sampling effect model. Conclusion: Our resul ...
... compared these with experimental microarray data generated from single embryonic neural stem cells in vivo. We show that the actual distribution of measured gene expression ratios for pairs of neural stem cells is much broader than that predicted from our sampling effect model. Conclusion: Our resul ...
Synthetic Zinc Finger Transcription Factor Action at
... luciferase reporter. The efficiency of activation under these circumstances at a defined site is dependent on DNA binding affinity. In contrast, only a subset of these same zinc finger proteins is able to activate the endogenous chromosomal locus. The activity of these proteins is influenced by thei ...
... luciferase reporter. The efficiency of activation under these circumstances at a defined site is dependent on DNA binding affinity. In contrast, only a subset of these same zinc finger proteins is able to activate the endogenous chromosomal locus. The activity of these proteins is influenced by thei ...