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No Slide Title - University of Vermont
No Slide Title - University of Vermont

Identification of Transcription Factor Binding Sites
Identification of Transcription Factor Binding Sites

... Using AlignACE 18 motifs from 12 clusters were found. 7 of the found motifs were identified experimentally ...
Ditto Chapter 15 Chromosomes
Ditto Chapter 15 Chromosomes

... 8. About 5% of individuals with Down Syndrome are the result of a chromosomal ...
Rethinking Gene Expression and Evolution (Nobel Lecture)
Rethinking Gene Expression and Evolution (Nobel Lecture)

... suggests how the genetic material can be replicated. They stated in their famously brief paper in Nature[1] that, “It has not escaped our notice that the specific pairing we have postulated immediately suggests a possible copying mechanism for the genetic material.” The DNA strands are wrapped aroun ...
Gene Enrichment Analysis
Gene Enrichment Analysis

... This lecture introduces the notion of enrichment analysis, where one wishes to assign biological meaning to some group of genes. Whereas in the past each gene product was studied individually to assign it functions and roles in biological processes, there now exist tools that allow this process to b ...
Mom and Dad are Fighting
Mom and Dad are Fighting

... allowing the father’s genes to act without any restraint. Children with Angelman try to nurse more than average children do. The smiles and laughter that are associated with Angelman may also come from attention-grabbing strategies encoded by paternal genes. Prader-Willi emerges from an opposite bre ...
The importance ofRNA
The importance ofRNA

... that compose it, while deoxyribonucleic acid (DNA) does not. This seemingly minor difference makes RNA much more flexible than DNA, resulting in a molecule that can adopt many different structures and acquire an array of functions. At the same time, RNA can in some cases use these hydroxyl groups to ...
Chapter 10: Genes and Chromosomes
Chapter 10: Genes and Chromosomes

Solid Tumour Section t(4;22)(q35;q12) in embryonal rhabdomyo-sarcoma (ERMS) Atlas of Genetics and Cytogenetics
Solid Tumour Section t(4;22)(q35;q12) in embryonal rhabdomyo-sarcoma (ERMS) Atlas of Genetics and Cytogenetics

... DUX4 Location 4q35 Protein DUX4 (double homeobox, chromosome 4) contains two homeodomains (about 60 amino acids, involved in DNA-binding), each similar in sequence to PAX3 and PAX7 homeodomains. It is a transcription factor DUX4 is involved in myogenic differentiation and cell-cycle control (Dixit e ...
Dosage compensation: do birds do it as well?
Dosage compensation: do birds do it as well?

... obviously contains many genes. For one of the nine genes analysed by quantitative RT–PCR, ScII, expression levels were consistently twice as high in males as in females [4]. This might suggest that some avian Z-linked genes escape dosage compensation in a similar manner to about 10% of X-linked huma ...
Methods S1.
Methods S1.

... fresh MS medium or MS supplemented with 200 mM NaCl. For kinetics of stress-mediated STRS relocalization, 10 day-old seedlings grown upon mesh on MS medium were transferred to a cover slip chamber (Nalge Nunc International) and flushed with liquid MS or MS supplemented with 200 mM NaCl, 500 mM manni ...
Lecture 5 pdf
Lecture 5 pdf

... Phenotype is result of complex, integrated pattern of reactions under control of more than one gene and the environment. 1. Epistasis (true non-allelic interaction) - expression of a single trait depends on interaction between 2 or more genes examples: comb shape in chickens ...
A Mini-Review
A Mini-Review

... protein is stabilized and regulated by nuclear lamina interactions [8]. Hence, the altered cell-cycle regulation observed in HGPS cells is probably due to an impaired function of the retinoblastoma protein network. Moreover, two recent in silico ceRNA and interactome analyses [9, 10] pointed to seve ...
Michigan State University Plant Genomics Program
Michigan State University Plant Genomics Program

Supplementary information
Supplementary information

... studied. Similarly, specific mouse mRNA species were measured relative to Pmm1 expression using mouse specific gene primers, listed in Supplementary Table 2. Invasion assays. MC38 cells were serum starved in 1% FBS media for 5 hrs prior to invasion assay. For invasion assays, 40l of 2 mg/ml matrige ...
Complex gene interactions in coat color
Complex gene interactions in coat color

Chem 465 Biochemistry II Hour Exam 3
Chem 465 Biochemistry II Hour Exam 3

... 1. Back in Chapter 24 it was stated that up to 45% of the human genome is made up of transposons. What is a transposon? How are simple and complex transposons different? What is the difference between direct transposition and replicative transposition? And, finally How are Eukaryotic transposons dif ...
The Murine Interleukin-3 Receptor a Subunit Gene
The Murine Interleukin-3 Receptor a Subunit Gene

... under the same condition except for annealing temperature at 55°C. The final amplified product was treated with T4 DNA polymerase to create blunt ends. After heat inactivation of polymerase, the DNA was cleaved with EcoRI, as the anchor sequence includes an EcoRI site. The resultant DNA fragments we ...
Microarray
Microarray

... Hypothesis testing is a common form of inferential statistics. A null hypothesis is stated, such as: “There is no difference in signal intensity for the gene expression measurements in normal and diseased samples.” The alternative hypothesis is that there is a difference. We use a test statistic to ...
Identification of porcine Lhx3 and SF1 as candidate genes for QTL
Identification of porcine Lhx3 and SF1 as candidate genes for QTL

... reactions (Neg) with no input cDNA also are shown. The assays for each stage were performed at least three times; a representative gel is shown. ...
Widespread Organ Expression of the Rat Proenkephalin Gene
Widespread Organ Expression of the Rat Proenkephalin Gene

... skeletal muscle is greater than that observed in neonatal brain (Fig. 2). Transcript abundance in muscle shows a steady several-fold decline during the first 2 weeks after birth and falls below the limits of detection in the adult (Fig. 3). In contrast, both brain and heart exhibited the opposite pa ...
Document
Document

... transcription and -10 and -35 boxes for this promoter are indicated in red above the sequence. P2 denotes the downstream promoter, recognized by σ E; the start of transcription and -10 and -35 boxes for this promoter are indicated in blue below the sequence. ...
TimeClust: a clustering tool for gene expression
TimeClust: a clustering tool for gene expression

... give indication about the optimal number of clusters thus the choice has to be quite arbitrary. BC, being a model-based evolution of the HC explicitly designed to consider the specific nature of temporal data, represents the most suitable algorithm for this kind of data. It is quite robust and compu ...
portable document (.pdf) format
portable document (.pdf) format

... a similar definition as univariate ranking (UR) as in Golub et al. (1999). Recently, Tomlins et al. (2005) introduced a method called “cancer outlier profile analysis” (COPA) for detecting “oncogene outliers.” Those genes show systematically increased expressions in disease samples, but only for a s ...
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Long non-coding RNA

Long non-coding RNAs (long ncRNAs, lncRNA) are non-protein coding transcripts longer than 200 nucleotides. This somewhat arbitrary limit distinguishes long ncRNAs from small regulatory RNAs such as microRNAs (miRNAs), short interfering RNAs (siRNAs), Piwi-interacting RNAs (piRNAs), small nucleolar RNAs (snoRNAs), and other short RNAs.
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