251 Lab 2 Chrisine
... Purpose: To search our sequence for the occurrence of any highly unusual repeat of a long word (> 3 nucleotides in length) The people who did the statistical analysis for the program BLAST (which we will begin using next week) said that it was below any reasonable level of statistical significance t ...
... Purpose: To search our sequence for the occurrence of any highly unusual repeat of a long word (> 3 nucleotides in length) The people who did the statistical analysis for the program BLAST (which we will begin using next week) said that it was below any reasonable level of statistical significance t ...
Tail DNA-Rapid Method - UMass Medical School
... When preparing samples of genomic DNA, use only those materials and solutions reserved for genomic use. These include Eppendorf tubes, proteinase K, dH2O, ethanol, and TE. When pipetting genomic DNA samples, only use pipette tips from which the tips have been snipped off (or purchased large-bore tip ...
... When preparing samples of genomic DNA, use only those materials and solutions reserved for genomic use. These include Eppendorf tubes, proteinase K, dH2O, ethanol, and TE. When pipetting genomic DNA samples, only use pipette tips from which the tips have been snipped off (or purchased large-bore tip ...
Topic 7 The Discovery of DNA & Its Roles
... Studied the T2 bacteriophage that infects E. coli T2 is a virus comprised of DNA & protein Infected E. coli produce new viruses; viral DNA OR protein is responsible 32P and protein with 35S Separately radiolabelled each component; DNA with Allowed infection to proceed, then blended & centrif ...
... Studied the T2 bacteriophage that infects E. coli T2 is a virus comprised of DNA & protein Infected E. coli produce new viruses; viral DNA OR protein is responsible 32P and protein with 35S Separately radiolabelled each component; DNA with Allowed infection to proceed, then blended & centrif ...
Chapter Fourteen ANSWERS TO REVIEW QUESTIONS All the
... 4. An allele frequency describes the proportion or percentage of a given allele in a population; phenotypic frequency, that of a particular phenotype; genotypic frequency the proportion or percentage of a particular genotype at a given locus. 5. Nonrandom mating, migration, genetic drift, mutation, ...
... 4. An allele frequency describes the proportion or percentage of a given allele in a population; phenotypic frequency, that of a particular phenotype; genotypic frequency the proportion or percentage of a particular genotype at a given locus. 5. Nonrandom mating, migration, genetic drift, mutation, ...
1. Explain why researchers originally thought
... 1. What does primase synthesize? A primer 2. Okazaki fragments make up which replicating strand? lagging Telomeres are special nucleotide sequences 3. _____ found at the ends of eukaryotic chromosomal DNA molecules. 4. Which proteins make up almost half of chromatin? histones 5. The less compacted, ...
... 1. What does primase synthesize? A primer 2. Okazaki fragments make up which replicating strand? lagging Telomeres are special nucleotide sequences 3. _____ found at the ends of eukaryotic chromosomal DNA molecules. 4. Which proteins make up almost half of chromatin? histones 5. The less compacted, ...
Here
... the genetics of the crops we plant. Although this section of the course is largely about genetically modified organisms (what we commonly refer to as the acronym, GMOs), one cannot understand genetic modification without understanding other breeding techniques, including induced-mutations and tradit ...
... the genetics of the crops we plant. Although this section of the course is largely about genetically modified organisms (what we commonly refer to as the acronym, GMOs), one cannot understand genetic modification without understanding other breeding techniques, including induced-mutations and tradit ...
BIO208 Bacterial Genetics Worksheet 1 1. . Fill in: Transformation
... a. _____________________________ DNA that binds repressor to prevent transcription b. _____________________________ DNA that binds RNA polymerase c. _________________________ binds repressor to allow transcription to occur d. _____________________________ a structural gene of the operon e. _________ ...
... a. _____________________________ DNA that binds repressor to prevent transcription b. _____________________________ DNA that binds RNA polymerase c. _________________________ binds repressor to allow transcription to occur d. _____________________________ a structural gene of the operon e. _________ ...
DNA Replication
... The process of making a copy of DNA is called DNA replication. The process can be broken down into three steps. Step 1: Before replication can begin, the double helix must unwind. This is accomplished by enzymes called DNA helicases, which open up the double helix by breaking the hydrogen bonds that ...
... The process of making a copy of DNA is called DNA replication. The process can be broken down into three steps. Step 1: Before replication can begin, the double helix must unwind. This is accomplished by enzymes called DNA helicases, which open up the double helix by breaking the hydrogen bonds that ...
Unit 5 : Cell Growth and Reproduction
... Ribosomes contain rRNA (ribosomal RNA) and protein. Steps to Translation: 1. mRNA attaches to a ribosome. 2. The ribosome reads the mRNA and tRNA (transfer RNA) will transfer the correct amino acid onto the chain to build a protein. • 3. The anticodon is the region on tRNA that is complementary to t ...
... Ribosomes contain rRNA (ribosomal RNA) and protein. Steps to Translation: 1. mRNA attaches to a ribosome. 2. The ribosome reads the mRNA and tRNA (transfer RNA) will transfer the correct amino acid onto the chain to build a protein. • 3. The anticodon is the region on tRNA that is complementary to t ...
7.014 Problem Set 3 Solutions
... iii. Model C: DNA is a double-stranded helix with sugar-phosphate backbones on the outside, and bases in the middle, where purines (A and G) pair with purines and pyrimidines (T and C) pair with pyrimidines. The strands are running anti-parallel to each other. This model should be rejected. This des ...
... iii. Model C: DNA is a double-stranded helix with sugar-phosphate backbones on the outside, and bases in the middle, where purines (A and G) pair with purines and pyrimidines (T and C) pair with pyrimidines. The strands are running anti-parallel to each other. This model should be rejected. This des ...
7.014 Problem Set 3 Solutions
... iii. Model C: DNA is a double-stranded helix with sugar-phosphate backbones on the outside, and bases in the middle, where purines (A and G) pair with purines and pyrimidines (T and C) pair with pyrimidines. The strands are running anti-parallel to each other. This model should be rejected. This des ...
... iii. Model C: DNA is a double-stranded helix with sugar-phosphate backbones on the outside, and bases in the middle, where purines (A and G) pair with purines and pyrimidines (T and C) pair with pyrimidines. The strands are running anti-parallel to each other. This model should be rejected. This des ...
Conjugative plasmids are circular pieces of DNA that not only
... Conjugative plasmids are circular pieces of DNA that not only replicate in host bacteria, but also encode machinery to transfer itself into bacteria that do not contain the plasmid. This process is termed conjugation. Nickase is an enzyme that initiates the transfer of the plasmid from “donor” bacte ...
... Conjugative plasmids are circular pieces of DNA that not only replicate in host bacteria, but also encode machinery to transfer itself into bacteria that do not contain the plasmid. This process is termed conjugation. Nickase is an enzyme that initiates the transfer of the plasmid from “donor” bacte ...
Public data and tool repositories Section 2 Survey of
... 1. Integrates feature identity information with whole genome view 2. Allows one to view and search an organism's complete genome 3. Displays chromosome maps 4. User can zoom into progressively greater levels of detail, down to the sequence data for a region of interest. 5. Focus more on individual s ...
... 1. Integrates feature identity information with whole genome view 2. Allows one to view and search an organism's complete genome 3. Displays chromosome maps 4. User can zoom into progressively greater levels of detail, down to the sequence data for a region of interest. 5. Focus more on individual s ...
File
... from replication fork b. Synthesized discontinuously in batches c. 5' 3' synthesis catalyzed by d. Segments called e. attaches fragment to lagging strand 7. Overall replication process is termed D. Stages of Replication in a Prokaryote 1. Initiation a. at an rich area within b. primed with replicati ...
... from replication fork b. Synthesized discontinuously in batches c. 5' 3' synthesis catalyzed by d. Segments called e. attaches fragment to lagging strand 7. Overall replication process is termed D. Stages of Replication in a Prokaryote 1. Initiation a. at an rich area within b. primed with replicati ...
Chapter 10 Nucleic Acids and Protein synthesis
... to form a strip of RNA. NOTE – Base pairing rules are the same as in DNA replication, EXCEPT URACIL REPLACES THYMINE!! Transcription continues until the RNA polymerase reaches a DNA region called the “TERMINATION SIGNAL” = specific sequence of nucleotides that marks the end of a gene or genes. NOTE ...
... to form a strip of RNA. NOTE – Base pairing rules are the same as in DNA replication, EXCEPT URACIL REPLACES THYMINE!! Transcription continues until the RNA polymerase reaches a DNA region called the “TERMINATION SIGNAL” = specific sequence of nucleotides that marks the end of a gene or genes. NOTE ...
Vocabulary to Know
... c. Does this person have a genetic disorder? If so, which one. 6. How are kayotypes used by genetic counselors? 7. Siblings are given up for adoption at birth and raised separately from one another for twenty-five years. When they meet for the first time, they realize that although they share the so ...
... c. Does this person have a genetic disorder? If so, which one. 6. How are kayotypes used by genetic counselors? 7. Siblings are given up for adoption at birth and raised separately from one another for twenty-five years. When they meet for the first time, they realize that although they share the so ...
DNA Workshop - Mrs. Sills` Science Site
... 6. Why does DNA replication occur? _________________________________________________ Please return to the original screen and click on “DNA Workshop Activity”. On the left of your screen click on “DNA Replication”. Click on “unzip the cell”. 7. Where does DNA replication occur? _____________________ ...
... 6. Why does DNA replication occur? _________________________________________________ Please return to the original screen and click on “DNA Workshop Activity”. On the left of your screen click on “DNA Replication”. Click on “unzip the cell”. 7. Where does DNA replication occur? _____________________ ...
Answers to Mastering Concepts Questions
... The DNA sample is divided among four test tubes, each containing the unknown sequence, primers, normal nucleotides (A, C, T, and G), replication enzymes, and a small amount of one type of terminator nucleotide. Replication results in different-sized fragments of complimentary copies of the unknown D ...
... The DNA sample is divided among four test tubes, each containing the unknown sequence, primers, normal nucleotides (A, C, T, and G), replication enzymes, and a small amount of one type of terminator nucleotide. Replication results in different-sized fragments of complimentary copies of the unknown D ...
Evidence of relationships between organisms
... Students should understand the following: • Genetic comparisons can be made between different species by direct examination of their DNA or of the proteins encoded by this DNA. • Comparison of DNA base sequences is used to elucidate relationships between organisms. These comparisons have led to new ...
... Students should understand the following: • Genetic comparisons can be made between different species by direct examination of their DNA or of the proteins encoded by this DNA. • Comparison of DNA base sequences is used to elucidate relationships between organisms. These comparisons have led to new ...
Finding genes
... tuberculosis PknB Ser/Thr protein kinase is . . . ELKL motif kinase 1. Query = the part of the PknB sequence that matches ELKL-1. Subject = ELKL-1. Expect = expectation value = the number of hits of this quality expected by chance in a database of this size (5e-24 = 5 x 10-24; is this a big number o ...
... tuberculosis PknB Ser/Thr protein kinase is . . . ELKL motif kinase 1. Query = the part of the PknB sequence that matches ELKL-1. Subject = ELKL-1. Expect = expectation value = the number of hits of this quality expected by chance in a database of this size (5e-24 = 5 x 10-24; is this a big number o ...
Assignment1 (50points)
... Assignment1 (50points) The goals of this exercise: * To assess the significance of the similarity between sequences (alignment, P ...
... Assignment1 (50points) The goals of this exercise: * To assess the significance of the similarity between sequences (alignment, P ...