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Sequence comparison of aflR from different Aspergillus species
Sequence comparison of aflR from different Aspergillus species

... biosynthetic pathway regulatory gene, aflR, which encodes a Cys6 Zn2 -type DNA-binding protein. Homologs of aflR from Aspergillus nomius, bombycis, parasiticus, flavus, and pseudotamarii were compared to investigate the molecular basis for variation among aflatoxin-producing taxa in the regulation of afl ...
The Drosophila pipsqueak gene encodes a nuclear BTB
The Drosophila pipsqueak gene encodes a nuclear BTB

... cells and somatically derived follicle cells throughout oogenesis and that it is required prior to stage one of oogenesis. PsqA contains a BTB (POZ) domain at its amino terminus; additionally, we have identified an evolutionarily conserved motif of unknown function present four times in tandem at th ...
Parallel Genetic and Phenotypic Evolution of DNA Superhelicity in
Parallel Genetic and Phenotypic Evolution of DNA Superhelicity in

... some viral and bacterial pathogens (Crandall et al. 1999; Reid et al. 2000). The parallel evolution of DNA superhelicity in the long-term experiment with E. coli provides therefore highly suggestive evidence that the resulting changes are adaptive. The change in DNA supercoiling was then further exa ...
and GvpD-mediated transcription regulation of the p
and GvpD-mediated transcription regulation of the p

... the reason for these differences. The amino acid sequence of GvpD indicates a conserved p-loop motif near the N-terminus typical of ATP/GTP binding proteins and important for the repressor function of GvpD (Pfeifer et al., 2001). The GvpD and GvpE proteins of Hfx. mediterranei are able to interact i ...
Neurobiology transfection guide
Neurobiology transfection guide

Propeller-twisted Adenin.Thymine and Guanine.Cytosine Base Pairs
Propeller-twisted Adenin.Thymine and Guanine.Cytosine Base Pairs

... which significantly deviates from zero in DNA crystal structures. It is furthermore a very interesting general observation that negative propellers dominate over positive ones in DNA (Dickerson 1988). We set the propeller to a number of fixed values within —50° and +50° in Watson-Crick C.G and T.A b ...
Gene Section LPP (lipoma preferred partner) Atlas of Genetics and Cytogenetics
Gene Section LPP (lipoma preferred partner) Atlas of Genetics and Cytogenetics

... shuttle between the nucleus and the cytoplasm, LPP and its family members (see below) have been proposed to be scaffolding proteins involved in signal transduction from sites of cell adhesion to the nucleus; LPP has been shown to harbour transcriptional activation capacity in luciferase reporter ass ...
IOSR Journal of Agriculture and Veterinary Science (IOSR-JAVS)
IOSR Journal of Agriculture and Veterinary Science (IOSR-JAVS)

... factors affect gene expression. Researchers have suggested that many enzymes such as lipase, PLC, protease, and RNase are putative virulence factors for Aeromonas sp. [14, 15] and these factors may interfere in hemolysin production, even hindered by a number of environmental factors or even growth c ...
Institutional Biosafety Committee (IBC) Guidelines
Institutional Biosafety Committee (IBC) Guidelines

... Office and the Asst. VPRC oversees the committee activities. We are currently working on an Executive memorandum covering IBC function and biological safety. What is the authority of the IBC? The IBC can approve or disapprove protocols, stop further research in non-compliant laboratories, make recom ...
double-strand break repair pathways in dna structure
double-strand break repair pathways in dna structure

... Research on DNA structure did not cease progression after the scientific revolutionary discovery by Watson and Crick in 1953 of the canonical B-form righthanded DNA double helix conformation (displayed on the top in Figure 1) (5). On the contrary, the field increased with many other findings in the ...
IOSR Journal of Dental and Medical Sciences (IOSR-JDMS)
IOSR Journal of Dental and Medical Sciences (IOSR-JDMS)

... Astanand Jugessur ,Fedik Rahimov , Jeffrey C.Murray Genetic variants in IRF6 and risk of facial clefts: single marker and haplotype-based analyses in a population-based case control study of facial clefts in Norway. 2008 DOI: 10.1002/gepi.20314 Alexandre R Viera , Joseph R Avila , Jill Harrington Me ...
the association of chloroplast dna with photosynthetic membrane
the association of chloroplast dna with photosynthetic membrane

... in the electron microscope they appear as in Figs. 6-8. They are bound by a membrane to which adhere varying numbers of grana lamellae. The number of grana lamellae seen in a single section will vary according to the section plane. The grana are partially disrupted due to the isolation in the 3-5 mM ...
The specificity of regulatory protein binding to DNA is due to a
The specificity of regulatory protein binding to DNA is due to a

... analysis is carried out assuming that association process is very fast and that dissociation of nonspecific complexes is a ratelimiting step in the recognition of a specific binding site on DNA. The calculations show that a ligand can recognize its specific binding site on DNA within a reasonably li ...
FEMS Microbiology Letters
FEMS Microbiology Letters

... revealed the existence of one PFO (encoded by nifJ) in Synechocystis 6803 and N. punctiforme but two different PFOs, encoded by nifJ1 and nifJ2, in Anabaena. Sequence comparison now indicates that all cyanobacterial PFOs are more closely related to those of anaerobes than to those of aerobes. Revers ...
Patterns of Segmental Duplication in the Human Genome
Patterns of Segmental Duplication in the Human Genome

... divided into 500-kb segments and Blasting was performed on all-against-all segments using the default parameters. In this study, we were interested in examining duplications with size  1 kb and sequence similarity  90%; we did not consider older duplications, because they are more difficult to def ...
Reconstruction of the original mycoflora in pelleted feed by PCR
Reconstruction of the original mycoflora in pelleted feed by PCR

... quantify fungal DNA in these feed types, PCR-based methods were applied. The DNA bands in SSCP gel were analyzed with the GELCOMPAR II program. The results show that the similarities of DNA band profiles from both feed types (ground vs. pellet) were > 85% up to 97.6%. The within-group variability o ...
Introduction to BLAST ppt
Introduction to BLAST ppt

... For detecting highly conserved regions (eg., genes) between two sequences (eg., genomes) ...
A DNA-sequence based phylogeny for triculine snails (Gastropoda
A DNA-sequence based phylogeny for triculine snails (Gastropoda

... pomatiopsid or hydrobiid taxon and so a member of another rissooidean taxon was chosen. For each data set a χ 2 -test for intertaxon variation in nucleotide frequency was performed using PAUP∗ v. 4.0b10 (Swofford, 2002). The data were tested for (substitution) saturation using plots of the numbers o ...
Speciation and Gene Flow between Snails of Opposite Chirality
Speciation and Gene Flow between Snails of Opposite Chirality

... almost nothing is known about the first stages in any embryo. Snails may be crucial tools for studying left-right asymmetry, because their chirality is determined at a very early cleavage, and because several species have morphological variation, so that the gene(s) involved can be mapped [6]. It is ...
Synapsis-Mediated Fusion of Free DNA Ends Forms Inverted Dimer Plasmids in Yeast.
Synapsis-Mediated Fusion of Free DNA Ends Forms Inverted Dimer Plasmids in Yeast.

... range of DNA concentrations. This observation was reproduced in several similar experiments with these plasmids or with the closely related plasmids, pSK 136 and pSK142 (shown in Figure 6A). As will be seen below, the recovery of Ura+ Leu+ transformants is due to theformation of heterozygous inverte ...
Curr. Microbiol. 42
Curr. Microbiol. 42

... cry7 (Fig. 1A). A necessary condition for defining a new gene is either different interval(s) among the five conserved blocks, as found in cry8 (Fig 1B) and in other cry genes [28, 29], or homology variation in conserved blocks. This method can be practical for a distinct cry group or for several gr ...
Transcription blockage by stable H-DNA analogs in
Transcription blockage by stable H-DNA analogs in

... versions of H-DNA have been experimentally detected, and several nomenclatures to distinguish these two isoforms of H-DNA were suggested (reviewed in (5)). Here we have only studied the reverse-Hoogsteen structure (see below), referring to it as ‘H-DNA’. The net energetic balance of base pairing (i. ...
A multiplex PCR assay for the identification of animal species
A multiplex PCR assay for the identification of animal species

... possible to apply the assay to samples whose DNA is highly degraded by heat following the treatment indicated by the EU Law for thermal processing of animal ingredients (134.4 – 141.9 8C and 3.03 – 4.03 bar for 24 min). This degradation has caused some drawbacks in PCR application as observed by Bot ...
FLUORESCENT PROTEIN IN Escherichia coli
FLUORESCENT PROTEIN IN Escherichia coli

... The search by scientists for novel antimicrobial drugs that are less prone to causing resistance has been diverted back to the antibiotics produced by nature itself. This search had a breakthrough when magainin, the first antimicrobial peptide (AMP), was isolated by Zasloff [1987] from African clawe ...
Application No. DIR 108 SUMMARY INFORMATION
Application No. DIR 108 SUMMARY INFORMATION

... (EPSPS) enzyme. EPSPS enzymes participate in a biosynthetic pathway found in both plants and microorganisms that is required for the synthesis of some essential amino acids. Most plant EPSPS enzymes are inhibited by glyphosate, which results in plant death due to the lack of essential amino acids. H ...
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Helitron (biology)

A helitron is a transposon found in eukaryotes that is thought to replicate by a so-called ""rolling-circle"" mechanism. This category of transposons was discovered by Vladimir Kapitonov and Jerzy Jurka in 2001. The rolling-circle process begins with a break being made at the terminus of a single strand of the helitron DNA. Transposase then sits at this break and at another break where the helitron targets as a migration site. The strand is then displaced from its original location at the site of the break and attached to the target break, forming a circlular heteroduplex. This heteroduplex is then resolved into a flat piece of DNA via replication. During the rolling-circle process, DNA can be replicated beyond the initial helitron sequence, resulting in the flanking regions of DNA being ""captured"" by the helitron as it moves to a new location.
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