Transformation Lab - Towson University
... environment. It has a single circular chromosome that contains about five million DNA base pairs, only 1/600th the haploid amount of DNA in a human cell. E.coli may also contain small circular DNA molecules called plasmids (1,000-200,000 DNA base pairs). These plasmids carry genetic information and ...
... environment. It has a single circular chromosome that contains about five million DNA base pairs, only 1/600th the haploid amount of DNA in a human cell. E.coli may also contain small circular DNA molecules called plasmids (1,000-200,000 DNA base pairs). These plasmids carry genetic information and ...
The tightly regulated promoter of the xanA gene of
... we have shown that the one from Neurospora crassa fully complements a xanA deletion (Cultrone et al., 2005). In this article we investigate whether this gene is subject to the same regulatory signals as all other enzymes of the purine degradation pathway. We observed that the promoter element of xan ...
... we have shown that the one from Neurospora crassa fully complements a xanA deletion (Cultrone et al., 2005). In this article we investigate whether this gene is subject to the same regulatory signals as all other enzymes of the purine degradation pathway. We observed that the promoter element of xan ...
Genomic rearrangements account for more than one
... range PCR experiments using primers located in the exons surrounding the deletions. In this way, we successfully amplified abnormal migrating fragments ranging in size from 6 to 9 kb in all three patients (Fig. 4). Subsequent narrowing of the breakpoint-containing regions was achieved by comparison ...
... range PCR experiments using primers located in the exons surrounding the deletions. In this way, we successfully amplified abnormal migrating fragments ranging in size from 6 to 9 kb in all three patients (Fig. 4). Subsequent narrowing of the breakpoint-containing regions was achieved by comparison ...
Chapter 1.
... conditions of moderate stringency. Typically primers are 9 or 10-mer oligonucleotides ...
... conditions of moderate stringency. Typically primers are 9 or 10-mer oligonucleotides ...
animal genetics
... Chromosomes are made up of tightly-coiled strands of DNA. DNA is a complex molecule composed of deoxyribose, phosphoric acid, and four bases. Individual genes are located in a fixed position (known as the loci) on the strands of DNA. Illustration of DNA Double Helix from Wikipedia. ...
... Chromosomes are made up of tightly-coiled strands of DNA. DNA is a complex molecule composed of deoxyribose, phosphoric acid, and four bases. Individual genes are located in a fixed position (known as the loci) on the strands of DNA. Illustration of DNA Double Helix from Wikipedia. ...
A method for fast database search for all k-nucleotide repeats
... A signi cant portion of DNA consists of repeating patterns of various sizes, from very small (one, two and three nucleotides) to very large (over 300 nucleotides). Although the functions of these repeating regions are not well understood, they appear important for understanding the expression, regul ...
... A signi cant portion of DNA consists of repeating patterns of various sizes, from very small (one, two and three nucleotides) to very large (over 300 nucleotides). Although the functions of these repeating regions are not well understood, they appear important for understanding the expression, regul ...
Down syndrome, trisomy 21, parental origin, PCR, D21S11 and
... from paediatric genetics department of The Children’s Hospital & Institute of Child Health, (CH&ICH) Lahore. Blood samples were collected in heparin containing tubes for karyotyping and in sterile sodium-EDTA vacutainer tubes for molecular detection of Down syndrome. Complete physical examination of ...
... from paediatric genetics department of The Children’s Hospital & Institute of Child Health, (CH&ICH) Lahore. Blood samples were collected in heparin containing tubes for karyotyping and in sterile sodium-EDTA vacutainer tubes for molecular detection of Down syndrome. Complete physical examination of ...
7.1 Introduction
... exist, and established that there are two stable mating types. Another important figure in early yeast genetics, Carl C. Lindegren, published the first genetic map of yeast in 1949 (2874). Lindegren also helped to lead the yeast field toward using heterothallic yeast strains (that is, those that are ...
... exist, and established that there are two stable mating types. Another important figure in early yeast genetics, Carl C. Lindegren, published the first genetic map of yeast in 1949 (2874). Lindegren also helped to lead the yeast field toward using heterothallic yeast strains (that is, those that are ...
Power Point
... Chromosomes are made up of tightly-coiled strands of DNA. DNA is a complex molecule composed of deoxyribose, phosphoric acid, and four bases. Individual genes are located in a fixed position (known as the loci) on the strands of DNA. Illustration of DNA Double Helix from Wikipedia. ...
... Chromosomes are made up of tightly-coiled strands of DNA. DNA is a complex molecule composed of deoxyribose, phosphoric acid, and four bases. Individual genes are located in a fixed position (known as the loci) on the strands of DNA. Illustration of DNA Double Helix from Wikipedia. ...
Analysis of expressed sequence tags from the Huperzia serrata leaf
... tetraketides (Morita et al. 2007, Wanibuchi et al. 2007). However, as of January 2009, there were only 10 nucleotide sequences from H . serrata available in the NCBI database. The limited information on the genetic contents of this plant triggered our efforts to construct a cDNA library from the H . ...
... tetraketides (Morita et al. 2007, Wanibuchi et al. 2007). However, as of January 2009, there were only 10 nucleotide sequences from H . serrata available in the NCBI database. The limited information on the genetic contents of this plant triggered our efforts to construct a cDNA library from the H . ...
Use of an exogenous plasmid standard and quantitative PCR to
... of 100 µl eluted DNA was quantified. This adjusted reaction sq is then corrected for inhibition of PCR (I) and efficiency of DNA recovery by the extraction protocol (E). The fraction of pGEM recovered after adding to eluted DNA is I, and the fraction of pGEM recovered after addition at the beginning ...
... of 100 µl eluted DNA was quantified. This adjusted reaction sq is then corrected for inhibition of PCR (I) and efficiency of DNA recovery by the extraction protocol (E). The fraction of pGEM recovered after adding to eluted DNA is I, and the fraction of pGEM recovered after addition at the beginning ...
Immortalization of T-Cells Is Accompanied by Gradual Changes in
... cells, overexpression of TERT or immortalization with a doxycyclineinducible system (TERT and SV40-TAg) resulted in telomere extension but did not prevent senescence-associated DNA methylation [19]. It was also noted that methylation patterns were maintained throughout both long-term culture and agi ...
... cells, overexpression of TERT or immortalization with a doxycyclineinducible system (TERT and SV40-TAg) resulted in telomere extension but did not prevent senescence-associated DNA methylation [19]. It was also noted that methylation patterns were maintained throughout both long-term culture and agi ...
fulltext - UmU DiVA portal
... cells, overexpression of TERT or immortalization with a doxycyclineinducible system (TERT and SV40-TAg) resulted in telomere extension but did not prevent senescence-associated DNA methylation [19]. It was also noted that methylation patterns were maintained throughout both long-term culture and agi ...
... cells, overexpression of TERT or immortalization with a doxycyclineinducible system (TERT and SV40-TAg) resulted in telomere extension but did not prevent senescence-associated DNA methylation [19]. It was also noted that methylation patterns were maintained throughout both long-term culture and agi ...
Distinguishing Different DNA Heterozygotes by
... including the targeted HFE mutation, 3 rare SNPs, and 2 rare compound heterozygotes. In all cases studied, different heterozygotes could be distinguished, including those within the same SNP class (1 ). At the factor V and HFE loci, compound heterozygotes were easily distinguished from “single” hete ...
... including the targeted HFE mutation, 3 rare SNPs, and 2 rare compound heterozygotes. In all cases studied, different heterozygotes could be distinguished, including those within the same SNP class (1 ). At the factor V and HFE loci, compound heterozygotes were easily distinguished from “single” hete ...
Maintenance of DNA Methylation during the Arabidopsis Life Cycle
... methylation of cytosine residues located in the direct repeats of the 59 region mediated by the DNA methyltransferase MET1 (Kinoshita et al., 2004). The transcriptionally active FWA maternal allele in endosperm is characterized by demethylation of the direct repeats. This demethylation may occur in ...
... methylation of cytosine residues located in the direct repeats of the 59 region mediated by the DNA methyltransferase MET1 (Kinoshita et al., 2004). The transcriptionally active FWA maternal allele in endosperm is characterized by demethylation of the direct repeats. This demethylation may occur in ...
Annotation mapping functions
... post.to corresponds directly to the from and to arguments in translate, with ”pre” for the translation before mapping and ”post” for the translation after mapping. In this example we used the org.Hs.eg.db to map the input from the symbol to Ensembl. As org.Hs.eg.db is centred around Entrez gene ids, ...
... post.to corresponds directly to the from and to arguments in translate, with ”pre” for the translation before mapping and ”post” for the translation after mapping. In this example we used the org.Hs.eg.db to map the input from the symbol to Ensembl. As org.Hs.eg.db is centred around Entrez gene ids, ...
pdf
... [Thompson et al., 1997]. Neighbor-joining trees were produced using distance matrix methods (PAUP 4.0, Sinauer Associates), and the reliability of the phylogenetic reconstructions (1000 replicates) was evaluated by bootstrapping. For descriptive purposes, nirS and nirK gene clusters were identified ...
... [Thompson et al., 1997]. Neighbor-joining trees were produced using distance matrix methods (PAUP 4.0, Sinauer Associates), and the reliability of the phylogenetic reconstructions (1000 replicates) was evaluated by bootstrapping. For descriptive purposes, nirS and nirK gene clusters were identified ...
Evolution of Mammalian KELL Blood Group Glycoproteins and
... N-glycosylation sites were conserved among the mammalian KELL proteins examined. Sequence alignments, key amino acid residues and conserved predicted secondary and tertiary structures were also studied, including active site residues, predicted disulfide forming Cys residues, cytoplasmic, transmembr ...
... N-glycosylation sites were conserved among the mammalian KELL proteins examined. Sequence alignments, key amino acid residues and conserved predicted secondary and tertiary structures were also studied, including active site residues, predicted disulfide forming Cys residues, cytoplasmic, transmembr ...
Rosalind Franklin and the Double Helix
... proposed by Watson, explains the identical size of the ladder rungs and also Erwin Chargaff's 1952 observation that G and C (and likewise A and T) are always present in DNA in approximately equal amounts. The consistent pairings, along with the irregular linear vertical sequence of the bases, underl ...
... proposed by Watson, explains the identical size of the ladder rungs and also Erwin Chargaff's 1952 observation that G and C (and likewise A and T) are always present in DNA in approximately equal amounts. The consistent pairings, along with the irregular linear vertical sequence of the bases, underl ...
Unexpectedly High Levels of Cryptic Diversity Uncovered by a
... have mainly been using the 16S rRNA gene instead [11], and few DNA barcoding studies on non-avian reptiles have been conducted so far, with still fewer on islands [12]. Consequently, there is a lack of DNA barcodes for reptiles even though they are one of the best models for evolutionary, biogeograp ...
... have mainly been using the 16S rRNA gene instead [11], and few DNA barcoding studies on non-avian reptiles have been conducted so far, with still fewer on islands [12]. Consequently, there is a lack of DNA barcodes for reptiles even though they are one of the best models for evolutionary, biogeograp ...
The Use of Cytochrome B Sequence Variation in Estimation of
... traditional classificationof the genus.Johnsonet al. (1988) find a groupcontainingmembers of the subgenera Vireo and Lanivireo. a lone taxon containing the species V. bellii and a division of the subgenusVireosylvainto a gilvusand an olivaceusgroup.To test theseresults independently, sequencedata fr ...
... traditional classificationof the genus.Johnsonet al. (1988) find a groupcontainingmembers of the subgenera Vireo and Lanivireo. a lone taxon containing the species V. bellii and a division of the subgenusVireosylvainto a gilvusand an olivaceusgroup.To test theseresults independently, sequencedata fr ...
Click
... page in several ways, but the quickest and easiest is to simply click on the [Gene summaries] link found beneath each hit (click). This takes you to any Gene page that exists at NCBI for the accessions in OMIM. Note that there are other resources that are directly accessible by these links with each ...
... page in several ways, but the quickest and easiest is to simply click on the [Gene summaries] link found beneath each hit (click). This takes you to any Gene page that exists at NCBI for the accessions in OMIM. Note that there are other resources that are directly accessible by these links with each ...
