Determination of phylo-group diversity of Escherichia coli in
... colonies were re-isolated onto fresh EMB plates until only metallic colonies were growing on the plates. The isolated metallic colonies from each sample were re-inoculated into a broth at 37 ºC. The broth was diluted to the McFarland Standard (1.5 x 108 CFU/mL) and the DNA was extracted using the Qi ...
... colonies were re-isolated onto fresh EMB plates until only metallic colonies were growing on the plates. The isolated metallic colonies from each sample were re-inoculated into a broth at 37 ºC. The broth was diluted to the McFarland Standard (1.5 x 108 CFU/mL) and the DNA was extracted using the Qi ...
Application to Modify an Approved Exempt or Notifiable Low Risk
... Please initial each of the following statements to indicate that you understand your responsibilities when dealing with GMOs and then sign the application form. I have read, considered and understand my responsibilities under the Gene Technology Act 2000 and agree to undertake the GMO dealing outlin ...
... Please initial each of the following statements to indicate that you understand your responsibilities when dealing with GMOs and then sign the application form. I have read, considered and understand my responsibilities under the Gene Technology Act 2000 and agree to undertake the GMO dealing outlin ...
A disproportionate role for mtDNA in DobzhanskyMuller
... small and highly conserved in size (typically between 14 and 18 kb) and gene content (13 protein coding genes, 2 rRNAs and 22 tRNAs). In contrast, plant and fungal mtDNAs are much larger (up to 2000 kb) and contain 120–140 genes (Schuster & Brennicke 1994). Plant mtDNA contains many repeated element ...
... small and highly conserved in size (typically between 14 and 18 kb) and gene content (13 protein coding genes, 2 rRNAs and 22 tRNAs). In contrast, plant and fungal mtDNAs are much larger (up to 2000 kb) and contain 120–140 genes (Schuster & Brennicke 1994). Plant mtDNA contains many repeated element ...
Plant Functional Genomics Plant Functional Genomics
... insert size cloning capacity is much lower than that of the YAC system, it is this limited cloning capacity that helps to prevent chimerism, because the inserts with sizes between 130–200 kb can be selected, while larger inserts, composed of two or more DNA fragments, are beyond the cloning capacity ...
... insert size cloning capacity is much lower than that of the YAC system, it is this limited cloning capacity that helps to prevent chimerism, because the inserts with sizes between 130–200 kb can be selected, while larger inserts, composed of two or more DNA fragments, are beyond the cloning capacity ...
Capstone project 2015 in pdf format
... We recently demonstrated resilience of dinoflagellates to DNA damage agents. The present project aims to isolate and characterize dinoflagellate mutants with different genome sizes. The project will involve cell biological and molecular techniques in the generation and monitoring of dinoflagellate l ...
... We recently demonstrated resilience of dinoflagellates to DNA damage agents. The present project aims to isolate and characterize dinoflagellate mutants with different genome sizes. The project will involve cell biological and molecular techniques in the generation and monitoring of dinoflagellate l ...
The photosynthetic apparatus of Prochlorococcus
... light-harvesting antenna systems of a single genus, Prochlorococcus. This organism, discovered only about ten years ago, is an extremely small, Chl b-containing cyanobacterium that sometimes constitutes up to 50 % of the photosynthetic biomass in the oceans. Various Prochlorococcus strains are known ...
... light-harvesting antenna systems of a single genus, Prochlorococcus. This organism, discovered only about ten years ago, is an extremely small, Chl b-containing cyanobacterium that sometimes constitutes up to 50 % of the photosynthetic biomass in the oceans. Various Prochlorococcus strains are known ...
Mechanistic Comparison of High-Fidelity and Error
... 1.1. Scope of This Review Discovered in the early 1970s, DNA polymerase β (Pol β) was the first mammalian DNA repair polymerase to be characterized. Its relatively small size (39 kD) and the fact that it lacks an exonuclease proofreading activity make it a tractable system for studying the mechanism ...
... 1.1. Scope of This Review Discovered in the early 1970s, DNA polymerase β (Pol β) was the first mammalian DNA repair polymerase to be characterized. Its relatively small size (39 kD) and the fact that it lacks an exonuclease proofreading activity make it a tractable system for studying the mechanism ...
Nucleic Acids exploringorigins.org - vtu-nptel
... edutube.org/.../video/structure-and-function-macromolecules-nucleic-aci... Jan 14, 2013 - Nucleic acid structure and all its gory details are the focus of this screencast. ... Edutube educational videos | Learn something new every day ... Watch Nucleic Acid Video www.ovguide.com/nucleic-acid-9202a8c ...
... edutube.org/.../video/structure-and-function-macromolecules-nucleic-aci... Jan 14, 2013 - Nucleic acid structure and all its gory details are the focus of this screencast. ... Edutube educational videos | Learn something new every day ... Watch Nucleic Acid Video www.ovguide.com/nucleic-acid-9202a8c ...
Towards Programmable Molecular Machines
... consumes another type of DNA strand which they call fuel strand. This new tweezer switches between the two states autonomously until all the fuel strands get used up. Conceptually, this makes it quite similar to an engine, though of course such molecular machines can not yet be used to perform usefu ...
... consumes another type of DNA strand which they call fuel strand. This new tweezer switches between the two states autonomously until all the fuel strands get used up. Conceptually, this makes it quite similar to an engine, though of course such molecular machines can not yet be used to perform usefu ...
Tissue- and Development-specific Expression of Multiple
... well as direct sequencing of PCR products yielded no evidence for additional nNOS mRNA species or further variations within the coding sequence, except for a previously described 102-bp insert between exon 16 and 17 (23) that was detectable in skeletal muscle and embryo (data not shown). Comparative ...
... well as direct sequencing of PCR products yielded no evidence for additional nNOS mRNA species or further variations within the coding sequence, except for a previously described 102-bp insert between exon 16 and 17 (23) that was detectable in skeletal muscle and embryo (data not shown). Comparative ...
The Genome of a Mongolian Individual Reveals
... We here also carried out the annotation of the genome draft using the pipeline developed by BGI (Li, Fan, et al. 2010). For repeats annotation, we used the RepeatModeler (1.0.5) to construct the de novo repeat library. We also used the RepeatMasker (version 3.3.0) (http://repeatmasker.org) with the ...
... We here also carried out the annotation of the genome draft using the pipeline developed by BGI (Li, Fan, et al. 2010). For repeats annotation, we used the RepeatModeler (1.0.5) to construct the de novo repeat library. We also used the RepeatMasker (version 3.3.0) (http://repeatmasker.org) with the ...
The Preservation and Persistence of Human DNA in Soil during
... human DNA in soil, as the mere presence of human DNA in the soil can provide useful information in a number of circumstances. For example, in the event that a clandestine grave is located but is devoid of a body, the recovery of trace amounts of human DNA of a high enough quality could provide infor ...
... human DNA in soil, as the mere presence of human DNA in the soil can provide useful information in a number of circumstances. For example, in the event that a clandestine grave is located but is devoid of a body, the recovery of trace amounts of human DNA of a high enough quality could provide infor ...
cDNA cloning, expression and chromosomal localization of the
... located on a protrusion of the protein (Eklund et al., 1991). So far, only two thioredoxins have been described in mammalian cells: Trx1 is a cytosolic protein with many different functions most of them depending on the redox active site (Holmgren, 1985) and Trx2 is a mitocondrial protein whose only ...
... located on a protrusion of the protein (Eklund et al., 1991). So far, only two thioredoxins have been described in mammalian cells: Trx1 is a cytosolic protein with many different functions most of them depending on the redox active site (Holmgren, 1985) and Trx2 is a mitocondrial protein whose only ...
DNA-based biosensor for the electrocatalytic determination of
... rent of NADH (de-los-Santos-Alvarez et al., 2006; Mano and Kuhn, 1999; Toh et al., 2003). This effect is not completely understood at the molecular level, although it is speculated that divalent cations can effectively counterbalance the negative charge of the catalyst favouring the approach of the ...
... rent of NADH (de-los-Santos-Alvarez et al., 2006; Mano and Kuhn, 1999; Toh et al., 2003). This effect is not completely understood at the molecular level, although it is speculated that divalent cations can effectively counterbalance the negative charge of the catalyst favouring the approach of the ...
Mammalian monogamy is not controlled by a single gene
... 95°C for 2 min, followed by 40 cycles of denaturation at 95°C for 1 min, primer annealing at 50°C for 1 min, sequence extension at 72°C for 1 min, and a final extension step at 72°C for 30 min. PCR products were separated on a 1.5% agarose gel, and length differences were scored by comparison with a ...
... 95°C for 2 min, followed by 40 cycles of denaturation at 95°C for 1 min, primer annealing at 50°C for 1 min, sequence extension at 72°C for 1 min, and a final extension step at 72°C for 30 min. PCR products were separated on a 1.5% agarose gel, and length differences were scored by comparison with a ...
The Nature of Life
... (b) Single strand of DNA. These geometric shapes and letters are simple symbols for the nucleotides in a small section of one chain of a DNA molecule. Genetic information is encoded in specific sequences of the four types of nucleotides (their names are abbreviated here as A, T, C, and G). ...
... (b) Single strand of DNA. These geometric shapes and letters are simple symbols for the nucleotides in a small section of one chain of a DNA molecule. Genetic information is encoded in specific sequences of the four types of nucleotides (their names are abbreviated here as A, T, C, and G). ...
B-Bolivia, an Allele of the Maize b1 Gene with Variable Expression
... averaged 20.9% colored kernels and those of GS averaged 62.1% colored kernels. In both cases, the plants grown from colored seeds produced a somewhat higher proportion of colored kernels, however this difference between the average values was not statistically significant. These results indicate tha ...
... averaged 20.9% colored kernels and those of GS averaged 62.1% colored kernels. In both cases, the plants grown from colored seeds produced a somewhat higher proportion of colored kernels, however this difference between the average values was not statistically significant. These results indicate tha ...
Analysis of Cross Sequence Similarities for Multiple - PolyU
... I-frame since the redundancy found between two consecutive frames is always significant when comparing with that found within the frame itself. In the field of DNA compression, current algorithms are analogous to intra-frame compression as redundant information is exploited only within one chromosom ...
... I-frame since the redundancy found between two consecutive frames is always significant when comparing with that found within the frame itself. In the field of DNA compression, current algorithms are analogous to intra-frame compression as redundant information is exploited only within one chromosom ...
Specific Combinations of Zein Genes and Genetic Backgrounds
... In the case of Bo2It, the mutant line from which the o2-Italian allele has been recovered and first described in the sixties (Nelson, 1967), a proper comparison is not possible because its O2 isogenic line is not available in any collection. The two GBs, W22 and Oh43, carrying the o2-676 allele (Auk ...
... In the case of Bo2It, the mutant line from which the o2-Italian allele has been recovered and first described in the sixties (Nelson, 1967), a proper comparison is not possible because its O2 isogenic line is not available in any collection. The two GBs, W22 and Oh43, carrying the o2-676 allele (Auk ...
PHI-Canto video tutorial text - PHI-base
... And we move onto Step5c adding an annotation term. # In this case to the curation type ‘PHI phenotype’. # I am looking for ‘unaffected pathogenicity’ in the search box # which is one of 9 upper level PHI phenotype outcome terms used in the preliminary annotation. Subsequent additional PHI phenotype ...
... And we move onto Step5c adding an annotation term. # In this case to the curation type ‘PHI phenotype’. # I am looking for ‘unaffected pathogenicity’ in the search box # which is one of 9 upper level PHI phenotype outcome terms used in the preliminary annotation. Subsequent additional PHI phenotype ...
Pourcel et al., Microbiology 2005
... The remarkable repetitive elements called CRISPRs (clustered regularly interspaced short palindromic repeats) consist of repeats interspaced with non-repetitive elements or ‘spacers’. CRISPRs are present in both archaea and bacteria, in association with genes involved in DNA recombination and repair ...
... The remarkable repetitive elements called CRISPRs (clustered regularly interspaced short palindromic repeats) consist of repeats interspaced with non-repetitive elements or ‘spacers’. CRISPRs are present in both archaea and bacteria, in association with genes involved in DNA recombination and repair ...
Genome Mapping in the Horse
... Our ability to map and sequence whole genomes is one of the most important developments in biological science. It will provide us with an unprecedented insight into the genetic background of phenotypic traits, such as disease resistance, reproduction and growth and also makes it feasible to study th ...
... Our ability to map and sequence whole genomes is one of the most important developments in biological science. It will provide us with an unprecedented insight into the genetic background of phenotypic traits, such as disease resistance, reproduction and growth and also makes it feasible to study th ...
Exploring Human Visual Pigments Copyright 2008, Gale Rhodes
... your search, in the colors that signify their BLAST scores, are located for you on the diagram. Notice that there are about 100 proteins (discovered so far, that is) that have 40% or more positives in alignment with red opsin. The opsins are members of the very large family of G protein-coupled rece ...
... your search, in the colors that signify their BLAST scores, are located for you on the diagram. Notice that there are about 100 proteins (discovered so far, that is) that have 40% or more positives in alignment with red opsin. The opsins are members of the very large family of G protein-coupled rece ...
The Mitochondrial Genome of Chara vulgaris
... mtDNA than Chaetosphaeridium mtDNA. The high similarity of codon usage observed for these mtDNAs also is seen at the level of stop codons. Five of the 11 Chara mitochondrial genes ending with TGA or TAG have counterparts with the same stop codon in Marchantia and/or angiosperm mtDNAs ( atp4, atp8, r ...
... mtDNA than Chaetosphaeridium mtDNA. The high similarity of codon usage observed for these mtDNAs also is seen at the level of stop codons. Five of the 11 Chara mitochondrial genes ending with TGA or TAG have counterparts with the same stop codon in Marchantia and/or angiosperm mtDNAs ( atp4, atp8, r ...