Engineered bacteriophage-defence systems in bioprocessing
... slowly but steadily increased over the course of the ongoing infection; these genes were considered late genes because their highest expression was at the end of the lytic cycle. The genes that were designated late #2 include those that encode proteins that are involved in host lysis (holin and endo ...
... slowly but steadily increased over the course of the ongoing infection; these genes were considered late genes because their highest expression was at the end of the lytic cycle. The genes that were designated late #2 include those that encode proteins that are involved in host lysis (holin and endo ...
Reverse Transcription PCR (RT-PCR): The Molecular
... latency that can last for several years. The proviral DNA is replicated along with the cellular DNA and can be inherited through many generations. The HIV proviral DNA contains the major genes common to all non-transducing retroviruses. These genes are gag, pol and env (see Figure 2). HIV also conta ...
... latency that can last for several years. The proviral DNA is replicated along with the cellular DNA and can be inherited through many generations. The HIV proviral DNA contains the major genes common to all non-transducing retroviruses. These genes are gag, pol and env (see Figure 2). HIV also conta ...
BioBank cDNA Manual
... The BioBank a high quality source of cDNA validated for use in real-time PCR experiments. The cDNA is reverse transcribed from high quality DNAse treated RNA from a variety of sources using an optimised blend of Oligo-dT and random nonamer primers. BioBank cDNA is therefore essentially free of genom ...
... The BioBank a high quality source of cDNA validated for use in real-time PCR experiments. The cDNA is reverse transcribed from high quality DNAse treated RNA from a variety of sources using an optimised blend of Oligo-dT and random nonamer primers. BioBank cDNA is therefore essentially free of genom ...
Next Generation Quantitative PCR
... as SYBR Green Dye, or special probes such as TaqMan probe. abm selection of EvaGreen qPCR MasterMixes and TaqProbe qPCR MasterMixes provides various options for both methods which also accommodates most qPCR thermal instrument models available on the market. ...
... as SYBR Green Dye, or special probes such as TaqMan probe. abm selection of EvaGreen qPCR MasterMixes and TaqProbe qPCR MasterMixes provides various options for both methods which also accommodates most qPCR thermal instrument models available on the market. ...
Maneeshi Prasad
... planning, organization, and regulation. • It plays an important role in the integration of sensory and mnemonic information and the regulation of intellectual function and action. • It is also involved in working memory. • Complex mental activities require additional cortical and subcortical circuit ...
... planning, organization, and regulation. • It plays an important role in the integration of sensory and mnemonic information and the regulation of intellectual function and action. • It is also involved in working memory. • Complex mental activities require additional cortical and subcortical circuit ...
Transcriptional analysis of the gene for glutamine synthetase II and
... GSI (encoded by glnA, Wray and Fisher 1988) is composed of 12 identical subunits and is similar to other bacterial GSs of type I. The heat-sensitive GSII (encoded by glnII; Hillemann et al. 1993) is an octamer and resembles the eukaryotic GS type. In S. coelicolor, both GSI activity and glnA express ...
... GSI (encoded by glnA, Wray and Fisher 1988) is composed of 12 identical subunits and is similar to other bacterial GSs of type I. The heat-sensitive GSII (encoded by glnII; Hillemann et al. 1993) is an octamer and resembles the eukaryotic GS type. In S. coelicolor, both GSI activity and glnA express ...
Quantitative genetics of gene expression during fruit fly - EMBL-EBI
... through which differences between genomes result in differences between whole organisms remain poorly understood. To bridge this gap between genotype and phenotype, we need to understand the consequences of genetic variation at the cellular level. A major factor in this is gene regulation, in which ...
... through which differences between genomes result in differences between whole organisms remain poorly understood. To bridge this gap between genotype and phenotype, we need to understand the consequences of genetic variation at the cellular level. A major factor in this is gene regulation, in which ...
Stage- and Tissue-Specific Expression of Ethylene Receptor
... (Zhou et al., 1996; corresponding to LeETR1) is not affected by ethylene treatment. RP-ERS1 is under environmental control, and the mRNA level increases after submergence at low O2 concentrations and at high CO2 concentrations (Voesenek et al., 1997; Vriezen et al., 1997). However, because there are ...
... (Zhou et al., 1996; corresponding to LeETR1) is not affected by ethylene treatment. RP-ERS1 is under environmental control, and the mRNA level increases after submergence at low O2 concentrations and at high CO2 concentrations (Voesenek et al., 1997; Vriezen et al., 1997). However, because there are ...
special - Microbiology
... The availability of a transformation system, and of readily isolable streptomycete plasmids and phages, led to the rapid development of DNA cloning systems (Bibb e t al., 1980; Suarez & Chater, 1980; Thompson e t al., 1980; reviewed by Hopwood e t al., 1987). The ability to clone streptomycete genes ...
... The availability of a transformation system, and of readily isolable streptomycete plasmids and phages, led to the rapid development of DNA cloning systems (Bibb e t al., 1980; Suarez & Chater, 1980; Thompson e t al., 1980; reviewed by Hopwood e t al., 1987). The ability to clone streptomycete genes ...
SPT3 interacts with TFIID to allow normal transcription in
... To study the defects caused by sptl5-21 by a genetic approach, a selection for suppressors was carried out. The selection for sptl 5-21 suppressors relied on the phenotypes of an sptl 5-21 strain carrying the insertion mutations his4-9173 and lys2-173R2. An SPT15 + strain that contains these inserti ...
... To study the defects caused by sptl5-21 by a genetic approach, a selection for suppressors was carried out. The selection for sptl 5-21 suppressors relied on the phenotypes of an sptl 5-21 strain carrying the insertion mutations his4-9173 and lys2-173R2. An SPT15 + strain that contains these inserti ...
Expression of p53 Target Genes in the Early Phase of Long
... experimental model of synaptic plasticity. We have studied the expression of at least 50 genes that are transcriptionally regulated by p53, as well as other genes that are related to p53-dependent processes, in the early phase of LTP. Within 30 min after Schaffer collaterals (SC) tetanization, incre ...
... experimental model of synaptic plasticity. We have studied the expression of at least 50 genes that are transcriptionally regulated by p53, as well as other genes that are related to p53-dependent processes, in the early phase of LTP. Within 30 min after Schaffer collaterals (SC) tetanization, incre ...
Fig. 1 - Repositorio Académico
... MA) for production of transgenic flies. For each transgene, at least three independent insertions were isolated and analyzed. 2.8. Mad-binding sites prediction In a series of previously known regulatory regions of Dpp target genes (training set), we identified overrepresented motifs using the software ...
... MA) for production of transgenic flies. For each transgene, at least three independent insertions were isolated and analyzed. 2.8. Mad-binding sites prediction In a series of previously known regulatory regions of Dpp target genes (training set), we identified overrepresented motifs using the software ...
Class17 1-31 Win16 Cell Cycle Notes
... The consensus promoter sequence of the Tau operon is ATTGCTGATC. Which of the following promoters is more likely to give high expression levels: ATTGGTGATC or AGTGCTTATT? (only one strand is shown) What are the molecular interactions that determine the level of expression in either [high glucose, hi ...
... The consensus promoter sequence of the Tau operon is ATTGCTGATC. Which of the following promoters is more likely to give high expression levels: ATTGGTGATC or AGTGCTTATT? (only one strand is shown) What are the molecular interactions that determine the level of expression in either [high glucose, hi ...
The Structures of DNA and RNA
... character and is capable of folding into a wealth of diverse tertiary structures. These structures are full of surprises, such as non-classical base pairs, base-backbone interactions, and knot-like configurations. Most remarkable of all, and of profound evolutionary significance, some RNA molecules ...
... character and is capable of folding into a wealth of diverse tertiary structures. These structures are full of surprises, such as non-classical base pairs, base-backbone interactions, and knot-like configurations. Most remarkable of all, and of profound evolutionary significance, some RNA molecules ...
Biomarker for Spinal Muscular Atrophy: Expression
... causing weakness and atrophy of proximal muscles (for review see [2]). All SMA patients have at least one copy of SMN2, an inverted gene duplication of SMN1 that is exclusively present in humans ([3]). However, SMN2 has a transcriptionally silent C to T mutation in exon 7. This mutation drastically ...
... causing weakness and atrophy of proximal muscles (for review see [2]). All SMA patients have at least one copy of SMN2, an inverted gene duplication of SMN1 that is exclusively present in humans ([3]). However, SMN2 has a transcriptionally silent C to T mutation in exon 7. This mutation drastically ...
Histones - scientia.global
... performed in this field. They demonstrated that SLBP is the major protein that binds the 3’ end of histone mRNA in both the nucleus and the cytoplasm and that it is required for histone pre-mRNA processing. They also showed that SLBP binds to the 3’ stem-loop end of histone mRNA in polyribosomes. Th ...
... performed in this field. They demonstrated that SLBP is the major protein that binds the 3’ end of histone mRNA in both the nucleus and the cytoplasm and that it is required for histone pre-mRNA processing. They also showed that SLBP binds to the 3’ stem-loop end of histone mRNA in polyribosomes. Th ...
REAL-TIME PCR KITS FOR DIAGNOSIS
... Real Time PCR (qPCR) has revolutionized biological and medical sciences by providing quantitative detection of specific nucleic acid sequences. Today, Real Time PCR is one of the primary choices of the clinicians and laboratory professionals for the diagnosis of the pathological diseases. Efficient ...
... Real Time PCR (qPCR) has revolutionized biological and medical sciences by providing quantitative detection of specific nucleic acid sequences. Today, Real Time PCR is one of the primary choices of the clinicians and laboratory professionals for the diagnosis of the pathological diseases. Efficient ...
Control of ribosome traffic by position-dependent
... transfer RNA (tRNA) concentration, the degree of tRNA charging and/or the strength of the interaction between tRNA and codon in the ribosomal A-site [10,12]. Here, we study the effect of the position-dependent choice of synonymous codon on the ribosome traffic in Escherichia coli, by using the model ...
... transfer RNA (tRNA) concentration, the degree of tRNA charging and/or the strength of the interaction between tRNA and codon in the ribosomal A-site [10,12]. Here, we study the effect of the position-dependent choice of synonymous codon on the ribosome traffic in Escherichia coli, by using the model ...
Modified uridine at wobble position in tRNA of
... cytidine (C), and uridine (U). Although modified nucleosides are present at many positions in tRNAs, two positions in the anticodon region, position 34 (wobble position) and position 37, show the largest variety of modified nucleosides. In Saccharomyces cerevisiae, the xm5U type of modified uridines ...
... cytidine (C), and uridine (U). Although modified nucleosides are present at many positions in tRNAs, two positions in the anticodon region, position 34 (wobble position) and position 37, show the largest variety of modified nucleosides. In Saccharomyces cerevisiae, the xm5U type of modified uridines ...
INTRODUCTION - Mount Holyoke College
... weak how mutant allele has to be used for the flies to survive to metamorphosis. The howr17 allele is an appropriate weak allele since most flies survive to metamorphosis and some form viable adult flies. The howr17 mutation was induced by imprecise excision of a transposable Pelement {Baehrecke, 19 ...
... weak how mutant allele has to be used for the flies to survive to metamorphosis. The howr17 allele is an appropriate weak allele since most flies survive to metamorphosis and some form viable adult flies. The howr17 mutation was induced by imprecise excision of a transposable Pelement {Baehrecke, 19 ...
Spider Silk - Consortium for Mathematics and its Applications
... Because of significant laboratory research, we already know the amino acid sequences for many silk proteins. Additionally, research suggests that the technology to manufacture spider silk is not too far off. But perhaps we could do even better. What if we changed the amino acid sequence? Could we fi ...
... Because of significant laboratory research, we already know the amino acid sequences for many silk proteins. Additionally, research suggests that the technology to manufacture spider silk is not too far off. But perhaps we could do even better. What if we changed the amino acid sequence? Could we fi ...
Computational Identification of Plant MicroRNAs and
... controlling development, particularly those of transcription factors and F-box proteins. However, plant miRNAs have conserved regulatory functions extending beyond development, in that they also target superoxide dismutases, laccases, and ATP sulfurylases. The expression of miR395, the sulfurylaseta ...
... controlling development, particularly those of transcription factors and F-box proteins. However, plant miRNAs have conserved regulatory functions extending beyond development, in that they also target superoxide dismutases, laccases, and ATP sulfurylases. The expression of miR395, the sulfurylaseta ...
Non-coding RNA
A non-coding RNA (ncRNA) is an RNA molecule that is not translated into a protein. Less-frequently used synonyms are non-protein-coding RNA (npcRNA), non-messenger RNA (nmRNA) and functional RNA (fRNA). The DNA sequence from which a functional non-coding RNA is transcribed is often called an RNA gene.Non-coding RNA genes include highly abundant and functionally important RNAs such as transfer RNAs (tRNAs) and ribosomal RNAs (rRNAs), as well as RNAs such as snoRNAs, microRNAs, siRNAs, snRNAs, exRNAs, and piRNAs and the long ncRNAs that include examples such as Xist and HOTAIR (see here for a more complete list of ncRNAs). The number of ncRNAs encoded within the human genome is unknown; however, recent transcriptomic and bioinformatic studies suggest the existence of thousands of ncRNAs., but see Since many of the newly identified ncRNAs have not been validated for their function, it is possible that many are non-functional. It is also likely that many ncRNAs are non functional (sometimes referred to as Junk RNA), and are the product of spurious transcription.