Trans-acting siRNA-mediated repression of ETTIN
... R. Scott Poethig* Mutations in the ARGONAUTE gene ZIPPY(ZIP)/AGO7 in Arabidopsis accelerate the juvenile-to-adult transition. A screen for mutations that suppress this precocious phenotype yielded alleles of two auxin-related transcription factors known to be upregulated in zip: ETTIN (ETT)/ARF3 and ...
... R. Scott Poethig* Mutations in the ARGONAUTE gene ZIPPY(ZIP)/AGO7 in Arabidopsis accelerate the juvenile-to-adult transition. A screen for mutations that suppress this precocious phenotype yielded alleles of two auxin-related transcription factors known to be upregulated in zip: ETTIN (ETT)/ARF3 and ...
Access Presentation
... - G/G mutation enhances binding of transcriptional activator Sp1 to P2 resulting in increase in Mdm2 transcription ...
... - G/G mutation enhances binding of transcriptional activator Sp1 to P2 resulting in increase in Mdm2 transcription ...
one-step and stepwise magnification of a bobbed lethal
... to account for ribosomal gene increase during magnification and provide an explanation for reversion of bb' chromosomes more rapidly than is permitted by single events of unequal sister strand exchange. During the course of this study we also analyzed several stepwise bb'" chromosomes. These stepwis ...
... to account for ribosomal gene increase during magnification and provide an explanation for reversion of bb' chromosomes more rapidly than is permitted by single events of unequal sister strand exchange. During the course of this study we also analyzed several stepwise bb'" chromosomes. These stepwis ...
Design considerations for highly specific and efficient
... functionality algorithm, we developed an optimized alignment program to perform rapid, flexible, and complete specificity analysis of crRNAs, including detection of gapped alignments. We have combined this comprehensive specificity check with our functionality algorithm to select and score highly sp ...
... functionality algorithm, we developed an optimized alignment program to perform rapid, flexible, and complete specificity analysis of crRNAs, including detection of gapped alignments. We have combined this comprehensive specificity check with our functionality algorithm to select and score highly sp ...
Causes that Make a Difference - Philsci
... generalizations that hold over as wide a range of conditions as possible. Indeed, many philosophers of science claim that physical scientists seek causal generalizations which hold over all possible conditions. There is an active debate concerning whether such universality is an appropriate ideal fo ...
... generalizations that hold over as wide a range of conditions as possible. Indeed, many philosophers of science claim that physical scientists seek causal generalizations which hold over all possible conditions. There is an active debate concerning whether such universality is an appropriate ideal fo ...
Simple identification of dominant p53 mutants by
... are unable to function as sequence-specific transcription factors, generally because they contain mutations in the DNA binding domain which reduce the affinity for DNA (3,4). The high level of p53 protein commonly seen in tumours probably reflects the presence of a persistent p53 activating signal a ...
... are unable to function as sequence-specific transcription factors, generally because they contain mutations in the DNA binding domain which reduce the affinity for DNA (3,4). The high level of p53 protein commonly seen in tumours probably reflects the presence of a persistent p53 activating signal a ...
Rapid Diversification of RNase A Superfamily Ribonucleases from
... Identification of Novel Ribonuclease Genes from the Bullfrog, Rana catesbeiana Shown in Fig. 1A is an alignment of the amino acid sequences of the 14 ribonucleases identified in three Rana species (R. catesbeiana, R. japonica, and R. pipiens). The novel sequences are those of the 200 series: rc-203, ...
... Identification of Novel Ribonuclease Genes from the Bullfrog, Rana catesbeiana Shown in Fig. 1A is an alignment of the amino acid sequences of the 14 ribonucleases identified in three Rana species (R. catesbeiana, R. japonica, and R. pipiens). The novel sequences are those of the 200 series: rc-203, ...
ABSTRACT Title of Document: PROGRAMMED
... The PTC is located at the bottom of the large cleft on the interface side of the large subunit underneath the central protuberance (Polacek & Mankin, 2005; Rodnina et al, 2006). The acceptor ends of the tRNAs meet at the bottom of the funnel-shaped active site above the entrance to the peptide exit ...
... The PTC is located at the bottom of the large cleft on the interface side of the large subunit underneath the central protuberance (Polacek & Mankin, 2005; Rodnina et al, 2006). The acceptor ends of the tRNAs meet at the bottom of the funnel-shaped active site above the entrance to the peptide exit ...
A role for interleukin-1 in epidermal differentiation
... Although human epidermis contains levels of interleukin1 (IL-1) up to 100 times higher than other tissues, the role of this cytokine in epidermal biology is unknown. Here, we show that interleukin-1 regulates the expression of mRNAs for two proteins associated with the differentiated phenotype of hu ...
... Although human epidermis contains levels of interleukin1 (IL-1) up to 100 times higher than other tissues, the role of this cytokine in epidermal biology is unknown. Here, we show that interleukin-1 regulates the expression of mRNAs for two proteins associated with the differentiated phenotype of hu ...
DYAD in meiotic chromosome organisation - Development
... probes which were labelled with 32P. In situ hybridisation was carried out as described earlier (Siddiqi et al., 2000) using antisense riboprobe synthesised from the complete coding region of the DYAD cDNA. Control experiments using sense RNA gave no signal (data not shown). ...
... probes which were labelled with 32P. In situ hybridisation was carried out as described earlier (Siddiqi et al., 2000) using antisense riboprobe synthesised from the complete coding region of the DYAD cDNA. Control experiments using sense RNA gave no signal (data not shown). ...
PDF - Oxford Academic - Oxford University Press
... MSA; then, using random shuffling of each column, calculates the statistical significance of the observed values (Z-scores); and finally, using the Bernoulli estimator procedure, finds those columns of the MSA that have the highest Z-scores (SDPs) and have the minimal probability to arise by chance. ...
... MSA; then, using random shuffling of each column, calculates the statistical significance of the observed values (Z-scores); and finally, using the Bernoulli estimator procedure, finds those columns of the MSA that have the highest Z-scores (SDPs) and have the minimal probability to arise by chance. ...
Structural Energetics of a RNA-DNA Hybrid
... Figure 1.1.3. RNA can adopt a greater variety of structures, including hairpin loops, bulges, internal loops, and single strands. Double-stranded RNA is typically in the Aform. The properties of nucleic acids play important roles in the effectiveness of gene expression. In the transfer of genetic in ...
... Figure 1.1.3. RNA can adopt a greater variety of structures, including hairpin loops, bulges, internal loops, and single strands. Double-stranded RNA is typically in the Aform. The properties of nucleic acids play important roles in the effectiveness of gene expression. In the transfer of genetic in ...
hag expression in Bacillus subtilis is both negatively
... determine purity and recovery of the protein. The purified protein, which showed a single band with purity greater than 95 % (data not shown), was used for raising polyclonal anti-FlgM antibodies in rabbits (by Bangalore Genei, Bangalore, India). We tested the ability of the anti-FlgM antibodies to ...
... determine purity and recovery of the protein. The purified protein, which showed a single band with purity greater than 95 % (data not shown), was used for raising polyclonal anti-FlgM antibodies in rabbits (by Bangalore Genei, Bangalore, India). We tested the ability of the anti-FlgM antibodies to ...
Quantitative expression of cholera toxin mRNA in Vibrio cholerae
... influence of toxR on CT production in the classical strain. Both classical and El Tor strains have been shown to express equivalent levels of ToxR. In contrast, the classical strain expresses more ToxT, which has a higher binding affinity to toxbox, resulting in higher expression of CT (González-Bo ...
... influence of toxR on CT production in the classical strain. Both classical and El Tor strains have been shown to express equivalent levels of ToxR. In contrast, the classical strain expresses more ToxT, which has a higher binding affinity to toxbox, resulting in higher expression of CT (González-Bo ...
of Lactobacillus pentosus
... Davis et al., 1984, Davis and Henderson, 1987). This xylE gene was not found within the cluster of xyl genes but is present on a separate locus on theE. coli chromosome. The xylR gene codes for a regulatory protein involved in the activation of expression of the xylose genes in the presence of D-xyl ...
... Davis et al., 1984, Davis and Henderson, 1987). This xylE gene was not found within the cluster of xyl genes but is present on a separate locus on theE. coli chromosome. The xylR gene codes for a regulatory protein involved in the activation of expression of the xylose genes in the presence of D-xyl ...
factor occupancy and gene expression Effects of
... Chromatin state may also play a role either in increasing TF occupancy at variants bound by multiple TFs, or in maintaining a state established by pioneer factors. In support of this hypothesis, the DNA near TF hubs had increased sensitivity to DNase I when compared with regions bound by a single fa ...
... Chromatin state may also play a role either in increasing TF occupancy at variants bound by multiple TFs, or in maintaining a state established by pioneer factors. In support of this hypothesis, the DNA near TF hubs had increased sensitivity to DNase I when compared with regions bound by a single fa ...
factor occupancy and gene expression Effects of sequence variation
... Chromatin state may also play a role either in increasing TF occupancy at variants bound by multiple TFs, or in maintaining a state established by pioneer factors. In support of this hypothesis, the DNA near TF hubs had increased sensitivity to DNase I when compared with regions bound by a single fa ...
... Chromatin state may also play a role either in increasing TF occupancy at variants bound by multiple TFs, or in maintaining a state established by pioneer factors. In support of this hypothesis, the DNA near TF hubs had increased sensitivity to DNase I when compared with regions bound by a single fa ...
factor occupancy and gene expression Effects of sequence variation
... Chromatin state may also play a role either in increasing TF occupancy at variants bound by multiple TFs, or in maintaining a state established by pioneer factors. In support of this hypothesis, the DNA near TF hubs had increased sensitivity to DNase I when compared with regions bound by a single fa ...
... Chromatin state may also play a role either in increasing TF occupancy at variants bound by multiple TFs, or in maintaining a state established by pioneer factors. In support of this hypothesis, the DNA near TF hubs had increased sensitivity to DNase I when compared with regions bound by a single fa ...
Gustav Fischer Verlag Stuttgart • New York
... methanogenic archaebacteria. B ' a n d B " w i t h i n one Anti - ß' ( E . c o l i ) (S. c e r e v i s i a e ) R N A polymerase are i m m u n o l o g i c a l l y unrelated, but both components crossreact w i t h the B subunit of the R N A polymerases of sulfur-dependent archaebacteria. — Subunit ß o ...
... methanogenic archaebacteria. B ' a n d B " w i t h i n one Anti - ß' ( E . c o l i ) (S. c e r e v i s i a e ) R N A polymerase are i m m u n o l o g i c a l l y unrelated, but both components crossreact w i t h the B subunit of the R N A polymerases of sulfur-dependent archaebacteria. — Subunit ß o ...
Fragaria multicipita - DigitalCommons@University of Nebraska
... standards used were PhiX174 RF HaeIII digest (Life Technologies) and 1.6 kb ladder (Fermentas AB, Vilnius, Lithuania). RFLP patterns were compared with those previously published (Davis et al., 1997; Jomantiene et al. 1998a,b, 2002; Lee et al. 1998). Phytoplasma 16S rRNA group and subgroup designati ...
... standards used were PhiX174 RF HaeIII digest (Life Technologies) and 1.6 kb ladder (Fermentas AB, Vilnius, Lithuania). RFLP patterns were compared with those previously published (Davis et al., 1997; Jomantiene et al. 1998a,b, 2002; Lee et al. 1998). Phytoplasma 16S rRNA group and subgroup designati ...
No Slide Title
... • Loss of RNAi reveals centromeric transcripts. • dsRNA from centromere targets transcriptional silencing. ...
... • Loss of RNAi reveals centromeric transcripts. • dsRNA from centromere targets transcriptional silencing. ...
Document
... VIROIDS: DID YOU KNOW VIROIDS… •are infectious particles. •are smaller than any of the known viruses. •range in size from approximately 20 nm (8 x 10-7 inches) ... •are just 100 times larger than Atoms which measure in at .1 nanometers. •cause certain plant and human diseases. •consists only of Nuc ...
... VIROIDS: DID YOU KNOW VIROIDS… •are infectious particles. •are smaller than any of the known viruses. •range in size from approximately 20 nm (8 x 10-7 inches) ... •are just 100 times larger than Atoms which measure in at .1 nanometers. •cause certain plant and human diseases. •consists only of Nuc ...
Computational Definition of
... A fundamental step in the transfer of genetic information from DNA to protein is the splicing of RNA transcripts. In this process relatively small exons (~100 nt) are selected from among generally much larger introns (1000’s of nt) and joined to form the mature mRNA. Pre-mRNA splicing is accomplishe ...
... A fundamental step in the transfer of genetic information from DNA to protein is the splicing of RNA transcripts. In this process relatively small exons (~100 nt) are selected from among generally much larger introns (1000’s of nt) and joined to form the mature mRNA. Pre-mRNA splicing is accomplishe ...
Robust gene silencing mediated by antisense small RNAs in the
... sRNAs in E. histolytica. We show for the first time that AS sRNAs directly mediate gene silencing. Our data demonstrate that a small portion of a gene-coding region to which large numbers of AS sRNAs map is sufficient to ‘trigger’ silencing of a gene fused to it. Gene silencing only occurred in E. his ...
... sRNAs in E. histolytica. We show for the first time that AS sRNAs directly mediate gene silencing. Our data demonstrate that a small portion of a gene-coding region to which large numbers of AS sRNAs map is sufficient to ‘trigger’ silencing of a gene fused to it. Gene silencing only occurred in E. his ...
Non-coding RNA
A non-coding RNA (ncRNA) is an RNA molecule that is not translated into a protein. Less-frequently used synonyms are non-protein-coding RNA (npcRNA), non-messenger RNA (nmRNA) and functional RNA (fRNA). The DNA sequence from which a functional non-coding RNA is transcribed is often called an RNA gene.Non-coding RNA genes include highly abundant and functionally important RNAs such as transfer RNAs (tRNAs) and ribosomal RNAs (rRNAs), as well as RNAs such as snoRNAs, microRNAs, siRNAs, snRNAs, exRNAs, and piRNAs and the long ncRNAs that include examples such as Xist and HOTAIR (see here for a more complete list of ncRNAs). The number of ncRNAs encoded within the human genome is unknown; however, recent transcriptomic and bioinformatic studies suggest the existence of thousands of ncRNAs., but see Since many of the newly identified ncRNAs have not been validated for their function, it is possible that many are non-functional. It is also likely that many ncRNAs are non functional (sometimes referred to as Junk RNA), and are the product of spurious transcription.