operon
... • The term inducer is used to describe any effector that turns on transcription of an inducible operon • The lac operon is an inducible operon because it is turned off unless induced © 2012 Pearson Education, Inc. ...
... • The term inducer is used to describe any effector that turns on transcription of an inducible operon • The lac operon is an inducible operon because it is turned off unless induced © 2012 Pearson Education, Inc. ...
VegT activates Bix4 to specify endodermal
... of Bix4 contains sites that bind VegT as well as Xbra (Tada et al., 1998). In this paper, we first show that expression of Bix4 requires maternal VegT and that VegT is a potent activator both of endogenous Bix4 and of reporter constructs driven by the Bix4 upstream regulatory region. Two T-box bindi ...
... of Bix4 contains sites that bind VegT as well as Xbra (Tada et al., 1998). In this paper, we first show that expression of Bix4 requires maternal VegT and that VegT is a potent activator both of endogenous Bix4 and of reporter constructs driven by the Bix4 upstream regulatory region. Two T-box bindi ...
Construction and Characterization of a Highly Regulable Expression
... conditions that induce protein production. Unfortunately, however, numerous researchers have found that these vectors produce significant amounts of protein even when grown under repressed conditions. We describe here a new expression vector, pLAC11, which was designed to be more regulable and thus ...
... conditions that induce protein production. Unfortunately, however, numerous researchers have found that these vectors produce significant amounts of protein even when grown under repressed conditions. We describe here a new expression vector, pLAC11, which was designed to be more regulable and thus ...
Cooperative Effects of Bacterial Mutations Affecting A N Gene
... growth of phage A, an effect primarily seen when Snu mutations are combined with another class of mutations, nus. Nus mutations have previously been shown to inhibit the expression of the N gene product of h, and the experiments reported here suggest that Snu mutations add to this inhibitory effect. ...
... growth of phage A, an effect primarily seen when Snu mutations are combined with another class of mutations, nus. Nus mutations have previously been shown to inhibit the expression of the N gene product of h, and the experiments reported here suggest that Snu mutations add to this inhibitory effect. ...
Linear Dominance Relationship among Four Class
... enzyme, resulting in a wrinkled-seed character (Bhattacharyya et al. 1990). To date, dominant/recessive alleles have been isolated and characterized for many genes related to several phenomena in various plant species (Glazebrook 1999, Szymanski et al. 2000, Theisen 2001). The biological phenomena r ...
... enzyme, resulting in a wrinkled-seed character (Bhattacharyya et al. 1990). To date, dominant/recessive alleles have been isolated and characterized for many genes related to several phenomena in various plant species (Glazebrook 1999, Szymanski et al. 2000, Theisen 2001). The biological phenomena r ...
Complete Characterization of the 3 Mouse Hereditary Hemochromatosis HFE Gene and
... discovered 3⬘ sequence, in order to perform RTPCR experiments to determine whether this novel EST contig sequence was, in fact, part of the HFE gene (Fig. 1, primers Ex6U and ESTL). Two bands were detected in several tissues (Fig. 2A), the upper band corresponding to the genomic sequence including t ...
... discovered 3⬘ sequence, in order to perform RTPCR experiments to determine whether this novel EST contig sequence was, in fact, part of the HFE gene (Fig. 1, primers Ex6U and ESTL). Two bands were detected in several tissues (Fig. 2A), the upper band corresponding to the genomic sequence including t ...
Comparative analysis of different TaqMan real-time RT
... HEV is transmitted by the faecal-oral route or contaminated water. It may occur in three different forms: large epidemics, smaller outbreaks or sporadic infections (Okamoto 2007). Symptoms of HEV infection are fever, ...
... HEV is transmitted by the faecal-oral route or contaminated water. It may occur in three different forms: large epidemics, smaller outbreaks or sporadic infections (Okamoto 2007). Symptoms of HEV infection are fever, ...
Iron-sulfur Clusters in RNA Polymerase: A New Role for an Ancient
... and Planetary Sciences, University of Arkansas, Fayetteville, AR 72701; 3Department of Botany and Microbiology, University of Oklahoma, Norman, OK 73019 Introduction Iron-sulfur (Fe-S) clusters are ubiquitous prosthetic groups that serve essential catalytic, structural, and regulatory functions in a ...
... and Planetary Sciences, University of Arkansas, Fayetteville, AR 72701; 3Department of Botany and Microbiology, University of Oklahoma, Norman, OK 73019 Introduction Iron-sulfur (Fe-S) clusters are ubiquitous prosthetic groups that serve essential catalytic, structural, and regulatory functions in a ...
Par-1
... machinery Many components of RNAi machinery have been identified through genetic screening for RNAi defective mutants and through biochemical studies using cell extracts (e.g. Drosophila embryo extract). ...
... machinery Many components of RNAi machinery have been identified through genetic screening for RNAi defective mutants and through biochemical studies using cell extracts (e.g. Drosophila embryo extract). ...
Molecular Design of Expression Systems
... upon interaction with a corresponding specific repressor protein. This interaction may be influenced by adjusting the temperature (ts regulatory mutant) or the composition (inducer or corepressor concentration) of the growth medium in order to switch from low to high promoter activity. Limitations o ...
... upon interaction with a corresponding specific repressor protein. This interaction may be influenced by adjusting the temperature (ts regulatory mutant) or the composition (inducer or corepressor concentration) of the growth medium in order to switch from low to high promoter activity. Limitations o ...
RNA interference - Bio
... ODNs- ODNs are generally ~20 nucleotides in length, their mode of action is by hybridizing to pre-mRNA and mRNA to produce a substrate for ribonuclease H (RNaseH) which specifically degrades the RNA strand of the formed RNA-DNA duplexes. Modification of ODN’s in a way to prevent the action of RNaseH ...
... ODNs- ODNs are generally ~20 nucleotides in length, their mode of action is by hybridizing to pre-mRNA and mRNA to produce a substrate for ribonuclease H (RNaseH) which specifically degrades the RNA strand of the formed RNA-DNA duplexes. Modification of ODN’s in a way to prevent the action of RNaseH ...
Pleiotropic effects of the mouse lethal yellow (Ay) mutation
... when homozygous (Dickie, 1962; Wolff et al., 1986). Finally, genetic complementation analysis suggests that there are multiple genes close to or within the agouti locus required for embryonic development [(Barsh and Epstein, 1989; Lyon et al., 1985; Russell et al., 1963); reviewed in Siracusa (1991) ...
... when homozygous (Dickie, 1962; Wolff et al., 1986). Finally, genetic complementation analysis suggests that there are multiple genes close to or within the agouti locus required for embryonic development [(Barsh and Epstein, 1989; Lyon et al., 1985; Russell et al., 1963); reviewed in Siracusa (1991) ...
Structure based hypothesis of a mitochondrial
... Recently, the hypothesis was put forward that mtRF1 recognizes adenine as the first base of nonstandard stop codons in vertebrate mitochondria [19]. However, this hypothesis seems to contradict the experimental observation that mtRF1a suffices to terminate all 13 human mitochondrial open reading fra ...
... Recently, the hypothesis was put forward that mtRF1 recognizes adenine as the first base of nonstandard stop codons in vertebrate mitochondria [19]. However, this hypothesis seems to contradict the experimental observation that mtRF1a suffices to terminate all 13 human mitochondrial open reading fra ...
RT2 Profiler™ PCR Arrays: Pathway
... 1. Specificity: Using BLAST and other algorithms, the specificity of each primer set is measured against the entire human, mouse, or rat genome to prevent the amplification of sequence-related, non-specific secondary products. The primer specificity is also checked against the E. coli genome to assure th ...
... 1. Specificity: Using BLAST and other algorithms, the specificity of each primer set is measured against the entire human, mouse, or rat genome to prevent the amplification of sequence-related, non-specific secondary products. The primer specificity is also checked against the E. coli genome to assure th ...
RT2 Profiler PCR Arrays: Pathway-focused gene
... RT² Profiler PCR Array, only primer pairs with similar GC content, melting temperature (Tm), and other ...
... RT² Profiler PCR Array, only primer pairs with similar GC content, melting temperature (Tm), and other ...
Relationships Between RNA Polymerase II Activity and Spt
... yeast genome and many of these exhibit modulation by many factors (Martens et al. 2004, 2005; Hainer et al. 2011; Bird et al. 2006). Furthermore, widespread antisense transcription is being revealed as a mechanism for shaping gene regulation in a number of ways, including transcription over promoter ...
... yeast genome and many of these exhibit modulation by many factors (Martens et al. 2004, 2005; Hainer et al. 2011; Bird et al. 2006). Furthermore, widespread antisense transcription is being revealed as a mechanism for shaping gene regulation in a number of ways, including transcription over promoter ...
Characterization of microbial activity in the surface layers of a
... the surrounding vegetation and the top layers of sediment are regularly mixed by infaunal bioturbation, wind-driven turbulence, and human activity such as the setting of crab traps. The top 5 cm of sediment used in these experiments was aerobic and appeared uniform in texture and color: fairly dense ...
... the surrounding vegetation and the top layers of sediment are regularly mixed by infaunal bioturbation, wind-driven turbulence, and human activity such as the setting of crab traps. The top 5 cm of sediment used in these experiments was aerobic and appeared uniform in texture and color: fairly dense ...
NanoString™: User Guide | nCounter® Expression Data Analysis
... Each miRGE CodeSet contains probes designed against sixteen ERCC transcript sequences. Six of these sequences are used as positive hybridization controls, two are used as ligation controls and eight are designed as negative controls. For each positive hybridization control, in-vitro transcribed RNA ...
... Each miRGE CodeSet contains probes designed against sixteen ERCC transcript sequences. Six of these sequences are used as positive hybridization controls, two are used as ligation controls and eight are designed as negative controls. For each positive hybridization control, in-vitro transcribed RNA ...
Page 4 of 4 - Thermo Fisher Scientific
... components of the product in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The buyer cannot sell or otherwise transfer (a) this product (b) its components or (c) materials made using this product or its components to a third party or otherwise use this prod ...
... components of the product in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The buyer cannot sell or otherwise transfer (a) this product (b) its components or (c) materials made using this product or its components to a third party or otherwise use this prod ...
Gene Section SRSF1 (serine/arginine rich splicing factor 1) -
... Online updated version : http://AtlasGeneticsOncology.org/Genes/SRSF1ID47631ch17q22.html ...
... Online updated version : http://AtlasGeneticsOncology.org/Genes/SRSF1ID47631ch17q22.html ...
1 Oviduct-embryo interactions in cattle
... the 8- to 16-cell stage) [1]. The developing embryo then enters the uterus where it soon forms a blastocyst, hatches from the zona pellucida and forms an ovoid, then tubular structure before undergoing elongation to form a filamentous conceptus that initiates implantation around Day 19 post concepti ...
... the 8- to 16-cell stage) [1]. The developing embryo then enters the uterus where it soon forms a blastocyst, hatches from the zona pellucida and forms an ovoid, then tubular structure before undergoing elongation to form a filamentous conceptus that initiates implantation around Day 19 post concepti ...
Multistep process of FUS aggregation in the cell cytoplasm involves
... enriched in these structures from unfixed GFP-FUS R522G transfected cells. The protein accumulated in Triton X-100insoluble fraction with time (Fig. 2E), and when lysates of cells collected 24 h post-transfection were treated with RNase A before fractionation, a dramatic shift of FUS from insoluble ...
... enriched in these structures from unfixed GFP-FUS R522G transfected cells. The protein accumulated in Triton X-100insoluble fraction with time (Fig. 2E), and when lysates of cells collected 24 h post-transfection were treated with RNase A before fractionation, a dramatic shift of FUS from insoluble ...
Leukaemia Section t(3;12)(q26;p13) ETV6/MECOM / t(3;12)(q26;p13) ETV6/EVI1 Atlas of Genetics and Cytogenetics
... The ETV6 gene spans a region of less than 250 kb at band 12p13.1 and consists of 8 exons. There are two start codons, one (exon 1a starting at codon 1) located at the beginning of the gene and another alternative (exon 1b starting at codon 43) upstream of exon 3. Protein The ETV6 protein (452 amino ...
... The ETV6 gene spans a region of less than 250 kb at band 12p13.1 and consists of 8 exons. There are two start codons, one (exon 1a starting at codon 1) located at the beginning of the gene and another alternative (exon 1b starting at codon 43) upstream of exon 3. Protein The ETV6 protein (452 amino ...
Genes for two multicopper proteins required for Fe(III) oxide
... Childers et al., 2002; Mehta et al., 2006; Reguera et al., 2005). One of these, designated outer-membrane protein B (OmpB), is a putative multicopper protein (Mehta et al., 2006) that is loosely associated with the outer cell surface (Qian et al., 2007), and is required for the reduction of insolubl ...
... Childers et al., 2002; Mehta et al., 2006; Reguera et al., 2005). One of these, designated outer-membrane protein B (OmpB), is a putative multicopper protein (Mehta et al., 2006) that is loosely associated with the outer cell surface (Qian et al., 2007), and is required for the reduction of insolubl ...
Differential Gene Expression in the Siphonophore
... from the same samples with three different off-the-shelf expression workflows: SOLiD SAGE (Life Technologies), Illumina mRNASeq, and Helicos Digital Gene Expression (DGE). These workflows differ in sample preparation protocols (Figure S1), sequencing platform, and read mapping. All these differences ...
... from the same samples with three different off-the-shelf expression workflows: SOLiD SAGE (Life Technologies), Illumina mRNASeq, and Helicos Digital Gene Expression (DGE). These workflows differ in sample preparation protocols (Figure S1), sequencing platform, and read mapping. All these differences ...
Non-coding RNA
A non-coding RNA (ncRNA) is an RNA molecule that is not translated into a protein. Less-frequently used synonyms are non-protein-coding RNA (npcRNA), non-messenger RNA (nmRNA) and functional RNA (fRNA). The DNA sequence from which a functional non-coding RNA is transcribed is often called an RNA gene.Non-coding RNA genes include highly abundant and functionally important RNAs such as transfer RNAs (tRNAs) and ribosomal RNAs (rRNAs), as well as RNAs such as snoRNAs, microRNAs, siRNAs, snRNAs, exRNAs, and piRNAs and the long ncRNAs that include examples such as Xist and HOTAIR (see here for a more complete list of ncRNAs). The number of ncRNAs encoded within the human genome is unknown; however, recent transcriptomic and bioinformatic studies suggest the existence of thousands of ncRNAs., but see Since many of the newly identified ncRNAs have not been validated for their function, it is possible that many are non-functional. It is also likely that many ncRNAs are non functional (sometimes referred to as Junk RNA), and are the product of spurious transcription.