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A missense mutation in growth differentiation factor 9 (GDF9) is
A missense mutation in growth differentiation factor 9 (GDF9) is

... Results: A QTL-region was identified on sheep chromosome 5, close to the growth differentiation factor 9 (GDF9), which is known to be a strong candidate gene for increased ovulation rate/litter size. Sequencing of the GDF9 coding region in the most extreme sires (high and low BLUP values) revealed a ...
Figures and figure supplements
Figures and figure supplements

... was detected in a large-budded cell (1). At the same time, GFP also appeared in the daughter cell that arose from the previous cell division (2). Due to the assymetry of switch 1 (only the mother cell remained green, implying that recombination occurred after DNA replication), switches 1 and 2 must ...
Brassinosteroids Rescue the Deficiency of CYP90, a Cytochrome
Brassinosteroids Rescue the Deficiency of CYP90, a Cytochrome

... consists of eight exons (see Figure 3A) with consensus splice sites at the exon–intron boundaries. The CPD cDNA showed over 90% homology with expressed sequence tags (ESTs) (e.g., EMBL accession number Z29017 and GenBank accession number T43151) from several organ-specific Arabidopsis cDNA libraries ...
Association of estrogen receptor β gene polymorphisms with bulimic
Association of estrogen receptor β gene polymorphisms with bulimic

... variants for this group of bulimic women. Identification of novel variants of the ERb gene The association described above could exist either because changes at the nucleotide positions in question affect ERb function or because they are in linkage disequilibrium with functional changes that might o ...
Silent polymorphisms in the RYR1 gene do not modify the
Silent polymorphisms in the RYR1 gene do not modify the

... Background: Central core disease is a congenital myopathy, characterized by presence of central core-like areas in muscle fibers. Patients have mild or moderate weakness, hypotonia and motor developmental delay. The disease is caused by mutations in the human ryanodine receptor gene (RYR1), which en ...
Light affects motility and infectivity of Agrobacterium tumefaciens
Light affects motility and infectivity of Agrobacterium tumefaciens

... grown A. tumefaciens. The proteins whose abundance varied between the different growth conditions at least twofold were identified by mass spectrometry. A list of proteins that appeared to be light regulated in three out of three independent experiments is given in Table 1. The level of six proteins ...
Different strategies of osmoadaptation in the closely
Different strategies of osmoadaptation in the closely

... E. coli, we deleted the gene otsB, which encodes trehalose-6phosphate phosphatase. The DNA regions upstream and downstream of otsB were joined by applying the SOE-PCR technique. The resulting DotsB fragment was cloned into the temperature-sensitive plasmid pMAK705, which facilitated the selection fo ...
Hemophilia A
Hemophilia A

... hemophilia A and only a small proportion of these mutations can be recognized by restriction endonuclease analysis, PCR and denaturing gradient gel electrophoresis (DGGE) were used to characterize single nucleotide substitutions.  A GC clamp was attached to the 5-prime PCR primer to allow detection ...
Supporting Information Parfenov et al. 10.1073/pnas.1416074111
Supporting Information Parfenov et al. 10.1073/pnas.1416074111

... mean-centered the RPKM values and divided by their SD for each exon. The lengths of the composite exons were transformed using the square root of the length. DNA Methylation and mRNA Expression Analysis. The Infinium HM450k array was used for TCGA Head and Neck samples (13). This platform includes p ...
SNP - Asia University, Taiwan
SNP - Asia University, Taiwan

... Aspects of frequency distribution • Population structure - example: SNP can be more frequent in one population than another. As migration is a potent (有效的) source of diversity, isolation affects the rate at which variation is lost (i.e. no variation) due to drift. • Nucleotide Diversity - the averag ...
New and Redesigned pRS Plasmid Shuttle Vectors for Genetic
New and Redesigned pRS Plasmid Shuttle Vectors for Genetic

... Heterologous dominant drug resistance markers, such as the kanMX module that confers resistance to G418 (Wach et al. 1994), provide some advantages over prototrophic biosynthetic markers for PCR-mediated gene disruption/deletion. Although using a prototrophic marker requires working with a strain th ...
a nine-base pair deletion distinguishes two en/spm
a nine-base pair deletion distinguishes two en/spm

... sequence represents the parental type and the a1-m (papu) is a derived type. [The molecular differences between al-m(dense) and al-m(Au) could not be determined as al-m(dense) is not available]. Origin of the 9-base pair deletion. How the deletion occurred is not known since no detectable change occ ...
Homeotic genes regulate the spatial expression
Homeotic genes regulate the spatial expression

... mutants (Immergliick et al. 1990). Thus, a key element of the control of differentiation by homeotic genes in the midgut, as well as in the epidermis, is the differential spatial expression pattern of each gene. The sequencing of wg revealed that its product is closely related to the product of the ...
The Tel-PDGFRß fusion gene produces a chronic
The Tel-PDGFRß fusion gene produces a chronic

... negative for the Philadelphia chromosome. A subset of CMML shows a distinctive t(5,12) chromosomal translocation, and shows features of both MDS and chronic myeloproliferative syndrome.4 Insight into CMML was obtained by the cloning and sequencing of the t(5;12) chromosomal breakpoint, associated wi ...
Charge Transport in DNA - Insights from
Charge Transport in DNA - Insights from

... DNA molecules were the initial impulses for the contemplations on the potential use of DNA-based devices in nano-electronics.[14, 15] Albeit, the structure of the DNA species in these experiments could not be resolved unambiguously. While it was concluded that the structure played a key role in dete ...
Atlas of Genetics and Cytogenetics in Oncology and Haematology  Scope
Atlas of Genetics and Cytogenetics in Oncology and Haematology Scope

... functions during differentiation; there is embryonic lethality of mice nullizygous for p300 (with defects in neurulation and heart development), and as well of mice double heterozygous for p300 and CBP, underlining their essential and associated role. ...
Chapter 1
Chapter 1

... microcytic hemolytic anemia. Beta thalassemia intermedia - Severity lies between the minor and major. Beta thalassemia major - homozygous disorder resulting in severe transfusiondependent hemolytic anemia. ...
Microarray data normalization and transformation
Microarray data normalization and transformation

... during labeling or hybridization; for example, if some genes preferentially label with the red or green dye. Let us assume we have two samples, A and B. In the first hybridization, we label A with our red dye and B with our green dye and reverse the dye labeling in the second, so that the ratios for ...
Failures in Mitochondrial tRNA and tRNA Metabolism
Failures in Mitochondrial tRNA and tRNA Metabolism

... America and the world. In particular, hypertension affects ⬇1 billion individuals worldwide and 130 million in China.1 The etiology of cardiovascular disease is not well understood because of the multifactorial causes. Cardiovascular disease can be caused by a single gene or multifactorial condition ...
PPTX - Tandy Warnow
PPTX - Tandy Warnow

... • MetaPhyler, MetaPhlAn, and mOTU are marker-based techniques (but use different marker genes). ...
Sickle Cell Anemia - University of Washington Department of
Sickle Cell Anemia - University of Washington Department of

... caused by a protozoan parasite (Plasmodium) that is transmitted to humans by the Anopheles mosquito. When malarial parasites invade the bloodstream, the red cells that contain defective hemoglobin become sickled and die, trapping the parasites inside them and reducing infection. Compared to AS heter ...
Detection of the Most Common Genetic Causes of
Detection of the Most Common Genetic Causes of

... 1A Y isoform gene (EIF1AY) and the RNA binding motif (RBM) family are found on AZFb region. EIF1AY encodes an essential translation factor. The PTP-BL-related Y (PRY) family of genes is mapped to AZFb and AZFc regions and encodes proteins proposed to be involved in apoptosis. RBM and deleted-in-azoo ...
Introductory genetics for veterinary students
Introductory genetics for veterinary students

...  eQTN has no significant “residual” effect on stature  No failure to quantitatively complement ...
PPR2263, a DYW-Subgroup Pentatricopeptide
PPR2263, a DYW-Subgroup Pentatricopeptide

... addition to numerous experimental localizations in plastids and mitochondria, nuclear (Ding et al., 2006) or dual localization to mitochondria and nuclei (Hammani et al., 2011) has been demonstrated experimentally. Whereas PPR proteins have been implicated in a wide range of biological processes, in ...
Opsin genes, cone photopigments, color vision, and color blindness
Opsin genes, cone photopigments, color vision, and color blindness

... Owing to the double-helical structure of DNA, the nucleotide in one DNA strand has a complementary nucleotide in the opposite strand. The two are held together, in nucleotide or base pairs (bp), by weak hydrogen bonds. Adenine (A) conjoins with thymine (T) and guanine (G) with cytosine (C); no other ...
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Therapeutic gene modulation

Therapeutic gene modulation refers to the practice of altering the expression of a gene at one of various stages, with a view to alleviate some form of ailment. It differs from gene therapy in that gene modulation seeks to alter the expression of an endogenous gene (perhaps through the introduction of a gene encoding a novel modulatory protein) whereas gene therapy concerns the introduction of a gene whose product aids the recipient directly.Modulation of gene expression can be mediated at the level of transcription by DNA-binding agents (which may be artificial transcription factors), small molecules, or synthetic oligonucleotides. It may also be mediated post-transcriptionally through RNA interference.
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