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... and previously reported low-lignin HCT- and COMT-antisense transgenic backgrounds (Guo et al., 2001a; Shadle et al., 2007). Lines showing the presence of the transgene in the genomic DNA were selected for further characterization, and transgenic lines transformed with empty vector were considered as ...
... and previously reported low-lignin HCT- and COMT-antisense transgenic backgrounds (Guo et al., 2001a; Shadle et al., 2007). Lines showing the presence of the transgene in the genomic DNA were selected for further characterization, and transgenic lines transformed with empty vector were considered as ...
Familial nonrandom inactivation linked to the X inactivation
... out subsequent cell divisions and, by this process, the dosage of proteins encoded by genes on the X chromosome is equalised in males and females. The choice of which of the two X chromosomes is the active one is usually random. Although stochastic events may account for a less than equal distributi ...
... out subsequent cell divisions and, by this process, the dosage of proteins encoded by genes on the X chromosome is equalised in males and females. The choice of which of the two X chromosomes is the active one is usually random. Although stochastic events may account for a less than equal distributi ...
A Conserved Molecular Framework for Compound Leaf Development
... search tree) from this multiple sequence alignment were generated with PhyML(2) using optimal amino acide substitution model JTT + G select using ModelGenerator (v0.84)(3). The NAC1 protein from A. thaliana was used as outgroup. Bootstrap confidence values were obtained by 100 replicates and only bo ...
... search tree) from this multiple sequence alignment were generated with PhyML(2) using optimal amino acide substitution model JTT + G select using ModelGenerator (v0.84)(3). The NAC1 protein from A. thaliana was used as outgroup. Bootstrap confidence values were obtained by 100 replicates and only bo ...
Sample final exam questions – BI/FS 430(H)/530
... 39. In “GM Crops: The Global and Economic and Environmental Impact - The First 9 Years” what are the main economic and environmental benefits claimed from nearly a decade of use of GM crops? Why does the article claim that GM crops have helped to reduced greenhouse gas emissions important to global ...
... 39. In “GM Crops: The Global and Economic and Environmental Impact - The First 9 Years” what are the main economic and environmental benefits claimed from nearly a decade of use of GM crops? Why does the article claim that GM crops have helped to reduced greenhouse gas emissions important to global ...
Genome Evolution Due to Allopolyploidization in Wheat
... silencing or duplication and pseudogenization (Levy and Feldman 2004; Feldman and Levy 2005, 2009; and references therein). We therefore have distinguished between revolutionary changes—which occur rapidly, within a few generations—and evolutionary changes, which take place gradually in the polyploi ...
... silencing or duplication and pseudogenization (Levy and Feldman 2004; Feldman and Levy 2005, 2009; and references therein). We therefore have distinguished between revolutionary changes—which occur rapidly, within a few generations—and evolutionary changes, which take place gradually in the polyploi ...
Why we have (only) five fingers per hand: Hox genes
... the anterior-posterior axis. However, the Hox-4 genes are also coordinately expressed in the CNS and elsewhere in the body mesenchyme. Thus to alter their expression would affect more than just the limb. In theory, the effects of a newly derived Hox-4 gene could be limited to the limbs by creating a ...
... the anterior-posterior axis. However, the Hox-4 genes are also coordinately expressed in the CNS and elsewhere in the body mesenchyme. Thus to alter their expression would affect more than just the limb. In theory, the effects of a newly derived Hox-4 gene could be limited to the limbs by creating a ...
Analysis of clones carrying repeated DNA sequences in two YAC
... main categories of repeated DNA sequences. This would enable chromosome walks into these highly repeated regions to be quickly recognized and terminated. YAC clones containing chimaeric inserts between repeated and unique DNA sequences could also be more readily identified. The stability of YAC clon ...
... main categories of repeated DNA sequences. This would enable chromosome walks into these highly repeated regions to be quickly recognized and terminated. YAC clones containing chimaeric inserts between repeated and unique DNA sequences could also be more readily identified. The stability of YAC clon ...
Advances in genetics show the need for extending screening
... (threshold for complex traits), since this means that a large number of individuals is needed for the analysis.15,16 Another means of identifying novel ADH genes is through genome-wide association studies. This approach received substantial interest in the last few years. For ADH, several new candid ...
... (threshold for complex traits), since this means that a large number of individuals is needed for the analysis.15,16 Another means of identifying novel ADH genes is through genome-wide association studies. This approach received substantial interest in the last few years. For ADH, several new candid ...
Photoreceptor Biotechnology Matthew Hudson Department of Crop
... this volume) and the B sensing phototropins (see Christie, this volume). The strategies used to influence desirable traits using these receptors are discussed in Section II. Of the three families, the phytochromes have so far attracted the most interest for biotechnology applications. This is in pa ...
... this volume) and the B sensing phototropins (see Christie, this volume). The strategies used to influence desirable traits using these receptors are discussed in Section II. Of the three families, the phytochromes have so far attracted the most interest for biotechnology applications. This is in pa ...
Fulltext PDF - Indian Academy of Sciences
... Plants that are vernalized as seeds or young seedlings do not flower immediately upon being raised to higher temperatures, but often weeks later. There is, therefore, a clear temporal separation between the perception of cold temperature and the switch from vegetative to reproductive growth. Somehow ...
... Plants that are vernalized as seeds or young seedlings do not flower immediately upon being raised to higher temperatures, but often weeks later. There is, therefore, a clear temporal separation between the perception of cold temperature and the switch from vegetative to reproductive growth. Somehow ...
Y chromosome azoospermia factor region microdeletions and
... kb [26] and it is specifically expressed in testis tissue [27]. This gene encodes an ATP dependent RNA helicase DEAD box protein in humans and plays a significant role in the pre-meiotic spermatogonia phase of spermatogenesis [27]. Molecular analysis revealed a high prevalence of deletions or mutati ...
... kb [26] and it is specifically expressed in testis tissue [27]. This gene encodes an ATP dependent RNA helicase DEAD box protein in humans and plays a significant role in the pre-meiotic spermatogonia phase of spermatogenesis [27]. Molecular analysis revealed a high prevalence of deletions or mutati ...
High-efficiency Agrobacterium-mediated transformation of chickpea
... appeared as a major limiting factor and the problem became more acute due to non-availability of sufficient amount of target tissues, competent enough for efficient integration of T-DNA from Agrobacterium or foreign DNA coated on microcarriers (Babaoglu et al. 2000; Somers et al. 2003). Somatic embr ...
... appeared as a major limiting factor and the problem became more acute due to non-availability of sufficient amount of target tissues, competent enough for efficient integration of T-DNA from Agrobacterium or foreign DNA coated on microcarriers (Babaoglu et al. 2000; Somers et al. 2003). Somatic embr ...
News from the west: Ancient DNA from a French megalithic burial
... Neolithic agriculturalists had limited impact on modern European female lineages. Setting aside the possibility of significant post-Neolithic migrations, they proposed a Paleolithic ancestry for modern Europeans. Nonetheless, two recent publications (Bramanti et al., 2009; Malmström et al., 2009) re ...
... Neolithic agriculturalists had limited impact on modern European female lineages. Setting aside the possibility of significant post-Neolithic migrations, they proposed a Paleolithic ancestry for modern Europeans. Nonetheless, two recent publications (Bramanti et al., 2009; Malmström et al., 2009) re ...
- Zurich Open Repository and Archive
... consecutive removal from chl of phytol and the Mg atom by chlorophyllase and a metal-chelating substance, respectively. Loss of the green color occurs during the subsequent opening of the porphyrin macrocycle of pheophorbide (pheide) a by the coupled action of pheide a oxygenase (PAO) and red chl c ...
... consecutive removal from chl of phytol and the Mg atom by chlorophyllase and a metal-chelating substance, respectively. Loss of the green color occurs during the subsequent opening of the porphyrin macrocycle of pheophorbide (pheide) a by the coupled action of pheide a oxygenase (PAO) and red chl c ...
PCDH19-related Infantile Epileptic Encephalopathy - HAL
... In contrast, females with heterozygous PCDH19 mutations present with early intractable seizures and a variable degree of mental retardation. PCDH19 is located in a region submitted to Xinactivation in females [Dibbens et al., 2008]. Random X-inactivation in mutated females is expected to lead to tis ...
... In contrast, females with heterozygous PCDH19 mutations present with early intractable seizures and a variable degree of mental retardation. PCDH19 is located in a region submitted to Xinactivation in females [Dibbens et al., 2008]. Random X-inactivation in mutated females is expected to lead to tis ...
NIH Public Access
... Since biochemical analysis of such a large, tightly chromatin bound RNA has been difficult by standard extraction-based approaches, we pursued a different approach to manipulate the binding or release of XIST RNA directly within living cells. This strategy built upon a significant clue from our earl ...
... Since biochemical analysis of such a large, tightly chromatin bound RNA has been difficult by standard extraction-based approaches, we pursued a different approach to manipulate the binding or release of XIST RNA directly within living cells. This strategy built upon a significant clue from our earl ...
The genetics of deafness - Archives of Disease in Childhood
... offspring risks, provided the syndrome has been recognised. Among the more familiar such syndromes are Waardenburg's syndrome and Branchio-Oto-Renal syndrome, both of which are inherited as autosomal dominant conditions, and Usher's syndrome, which is inherited in an autosomal recessive fashion. By ...
... offspring risks, provided the syndrome has been recognised. Among the more familiar such syndromes are Waardenburg's syndrome and Branchio-Oto-Renal syndrome, both of which are inherited as autosomal dominant conditions, and Usher's syndrome, which is inherited in an autosomal recessive fashion. By ...
ANALYSIS OF MULTIPLE RESTRICTION FRAGMENT LENGTH
... probes and containing an insert of 14 .5 kb, termed XGSB 16.1, was restriction mapped (Fig. 1 B) . The Pvu II-Bam HI fragment, GB2PE, which contained neither human repeats nor CRI-1- and CRI-4-like sequences, hybridized to the 14.5-kb Bam HI fragment characteristic ofan S allele and to nonallelic fr ...
... probes and containing an insert of 14 .5 kb, termed XGSB 16.1, was restriction mapped (Fig. 1 B) . The Pvu II-Bam HI fragment, GB2PE, which contained neither human repeats nor CRI-1- and CRI-4-like sequences, hybridized to the 14.5-kb Bam HI fragment characteristic ofan S allele and to nonallelic fr ...
Defining characteristics of Tn5 Transposase non
... and restriction enzymes, the importance of nonspecific DNA interactions for transposases has not been investigated. Here, we discuss non-specific DNA-Tn5 Transposase (Tnp) interactions and suggest how they stabilize the Tnp and modulate Tnp localization of the 19 bp Tnp recognition end sequences (ES ...
... and restriction enzymes, the importance of nonspecific DNA interactions for transposases has not been investigated. Here, we discuss non-specific DNA-Tn5 Transposase (Tnp) interactions and suggest how they stabilize the Tnp and modulate Tnp localization of the 19 bp Tnp recognition end sequences (ES ...
Genome Jigsaw: Implications of 16S Ribosomal RNA Gene
... may be effective for measuring diversity; however, the variable region source of these small fragments may require higher or lower id thresholds. How precisely should the pieces of this ‘genomic jigsaw’ be characterized and distinguished? Two algorithms, UCLUST and CD-HIT-EST, were used to cluster c ...
... may be effective for measuring diversity; however, the variable region source of these small fragments may require higher or lower id thresholds. How precisely should the pieces of this ‘genomic jigsaw’ be characterized and distinguished? Two algorithms, UCLUST and CD-HIT-EST, were used to cluster c ...
The Isolation of Mutagen-Sensitive nuv Mutants of
... and KELLY1987; FRIEDBERC 1988, 1991). Work with filamentous fungi has progressed much more slowly such that only a relatively small number of such mutants have been isolated and characterized. In Aspergillus nidulans only nine UV-sensitive (uvs) mutants have previously been extensively characterized ...
... and KELLY1987; FRIEDBERC 1988, 1991). Work with filamentous fungi has progressed much more slowly such that only a relatively small number of such mutants have been isolated and characterized. In Aspergillus nidulans only nine UV-sensitive (uvs) mutants have previously been extensively characterized ...
Spatially restricted domains of homeo-gene
... a segmentally restricted manner and that their expression presages morphological differentiation of segmental structures (e.g. Levine et a|.1983; Akam & Martinez-Arias, 1985; Chadwick & McGinnis, 1987). The discovery of homeo-genes in a wide variety of metazoz, including vertebrates, led to the sugg ...
... a segmentally restricted manner and that their expression presages morphological differentiation of segmental structures (e.g. Levine et a|.1983; Akam & Martinez-Arias, 1985; Chadwick & McGinnis, 1987). The discovery of homeo-genes in a wide variety of metazoz, including vertebrates, led to the sugg ...
Lactococcus lactis LM0230 contains a single
... prepared containing all 20 common amino acids at 50 µg ml−" except where stated. Additionally, M9 medium was supplemented with 20 µg adenine, guanine, uracil and xanthine ml−". L. lactis LM0230 was routinely grown at 30 mC in M17 (Terzaghi & Sandine, 1975) supplemented with 0n5 % (w\v) glucose (M17- ...
... prepared containing all 20 common amino acids at 50 µg ml−" except where stated. Additionally, M9 medium was supplemented with 20 µg adenine, guanine, uracil and xanthine ml−". L. lactis LM0230 was routinely grown at 30 mC in M17 (Terzaghi & Sandine, 1975) supplemented with 0n5 % (w\v) glucose (M17- ...
ANOVA and the Bootstrap - Computational Diagnostics Group
... 2. If the null is rejected, calculate a 95% confidence interval for a and assess based on the confidence interval if the effect is relevant. The linear effect was estimated as a=0.024. If this effect could be extrapolated to longer time intervals, it would take 30 days to half the expression level m ...
... 2. If the null is rejected, calculate a 95% confidence interval for a and assess based on the confidence interval if the effect is relevant. The linear effect was estimated as a=0.024. If this effect could be extrapolated to longer time intervals, it would take 30 days to half the expression level m ...
... After hybridization, the hybrid genome underwent extensive chromosomal rearrangements, including chromosome losses and the generation of chimeric chromosomes by the nonreciprocal recombination between homeologous chromosomes. These nonreciprocal recombinations between homeologous chromosomes occurre ...