Introduction to sequence similarity searches and sequence
... Compares the amino acid query sequence with the amino acid sequences in the database. Translates the database nucleotide sequences into all six frames and compares the resulting amino acid sequences with the amino acid query sequences. tfasty allows intra-codon substitutions and frameshifts. Transla ...
... Compares the amino acid query sequence with the amino acid sequences in the database. Translates the database nucleotide sequences into all six frames and compares the resulting amino acid sequences with the amino acid query sequences. tfasty allows intra-codon substitutions and frameshifts. Transla ...
Slide 1
... Read the chapter 7 in your text book, Essential Cell Biology (3rd Edition) Questions to [email protected], Rm 3114 ...
... Read the chapter 7 in your text book, Essential Cell Biology (3rd Edition) Questions to [email protected], Rm 3114 ...
MYbaits v2 manual
... If quantitative PCR is used to monitor enrichment, primer pairs should be designed such that the amplicon length is longer than the bait length or such that both primers cannot bind to a particular bait sequence. The RNA baits may contain trace amounts of DNA template used during production that cou ...
... If quantitative PCR is used to monitor enrichment, primer pairs should be designed such that the amplicon length is longer than the bait length or such that both primers cannot bind to a particular bait sequence. The RNA baits may contain trace amounts of DNA template used during production that cou ...
Lab 7: Molecular Biology
... Purified restriction enzymes are used by molecular biologists to cut large DNA molecules into smaller and more manageable pieces. An important property of restriction enzymes is that the ends of DNA fragments generated by restriction enzyme digestion have singlestranded extensions that are complemen ...
... Purified restriction enzymes are used by molecular biologists to cut large DNA molecules into smaller and more manageable pieces. An important property of restriction enzymes is that the ends of DNA fragments generated by restriction enzyme digestion have singlestranded extensions that are complemen ...
Using Total Internal Reflection Fluorescence Microscopy, DNA
... membranes [5-6], but these early TIRFM experiments were not yet able to resolve individual molecules. The use of TIRFM for visualizing single fluorescent molecules was brought about through significant advances in charge-coupled device (CCD) technologies that greatly enhanced the ability of these ca ...
... membranes [5-6], but these early TIRFM experiments were not yet able to resolve individual molecules. The use of TIRFM for visualizing single fluorescent molecules was brought about through significant advances in charge-coupled device (CCD) technologies that greatly enhanced the ability of these ca ...
Gene7-28
... overlapping proteins. Domain 1 is present in all proteins, domain 2 in the 289 and 243 residue proteins, and domain 3 is unique to the 2The adenovirus E1A region is spliced to form three transcripts that code for overlapping proteins. Domain 1 is present in all proteins, domain 2 in the 289 and 243 ...
... overlapping proteins. Domain 1 is present in all proteins, domain 2 in the 289 and 243 residue proteins, and domain 3 is unique to the 2The adenovirus E1A region is spliced to form three transcripts that code for overlapping proteins. Domain 1 is present in all proteins, domain 2 in the 289 and 243 ...
Control of Cell Division: Models from
... This separation of various DNA components, precise in time antl quantity, cannot be arranged by chance, as with cytoplasmic contents. The most attractive model assumes that the mechanism for segregation of genetic material at bacterial di vision is very like the one observed with cells of higher or ...
... This separation of various DNA components, precise in time antl quantity, cannot be arranged by chance, as with cytoplasmic contents. The most attractive model assumes that the mechanism for segregation of genetic material at bacterial di vision is very like the one observed with cells of higher or ...
to 3 - NUAMESAPBio
... ▪ The discovery of the genetic role of DNA began with research by Frederick Griffith in 1928 ▪ Griffith worked with two strains of a bacterium, one pathogenic and one harmless ▪ When he mixed heat-killed remains of the pathogenic strain with living cells of the harmless strain, some living cells bec ...
... ▪ The discovery of the genetic role of DNA began with research by Frederick Griffith in 1928 ▪ Griffith worked with two strains of a bacterium, one pathogenic and one harmless ▪ When he mixed heat-killed remains of the pathogenic strain with living cells of the harmless strain, some living cells bec ...
Deletion of Exon 4 in the N-Acetylgalactosamine-4 - J
... Neither PCR nor qPCR produced a signal for exon 4 when using genomic DNA from the patient studied; alternative splicing was not detected at the RNA level. We expect that the break/junction point for this deletion is located within the intron regions that flank exon 4. However, because exons 3 and 5 ...
... Neither PCR nor qPCR produced a signal for exon 4 when using genomic DNA from the patient studied; alternative splicing was not detected at the RNA level. We expect that the break/junction point for this deletion is located within the intron regions that flank exon 4. However, because exons 3 and 5 ...
Molecular Biology of the Cell
... The well-characterized MyoD gene encoded a master transcriptional regulatory protein for muscular gene promoters, including itself. The active chromatin surrounding the MyoD promoter contains the variant histone H3.3 in a Lys 4 methylate form. By injecting excess mRNA encoding normal H3.3 or mutant ...
... The well-characterized MyoD gene encoded a master transcriptional regulatory protein for muscular gene promoters, including itself. The active chromatin surrounding the MyoD promoter contains the variant histone H3.3 in a Lys 4 methylate form. By injecting excess mRNA encoding normal H3.3 or mutant ...
Document
... that allows the plasmid to replicate in the bacteria using the host DNA synthesis enzymes A promoter sequence for initiating transcription of the inserted gene A gene encoding a protein for antibiotic resistance, which allows for identification of bacteria that have taken in the plasmid ...
... that allows the plasmid to replicate in the bacteria using the host DNA synthesis enzymes A promoter sequence for initiating transcription of the inserted gene A gene encoding a protein for antibiotic resistance, which allows for identification of bacteria that have taken in the plasmid ...
Engineering of robust synthetic gene networks with the help of
... The main players are Transcription Factors (TFs) that regulate the expression of 100-1000 of genes when necessary. ...
... The main players are Transcription Factors (TFs) that regulate the expression of 100-1000 of genes when necessary. ...
Leukaemia Section t(11;17)(q23;q21) Atlas of Genetics and Cytogenetics in Oncology and Haematology
... In patients with t(11;17)(q23;q21), t(5;17)(q35;q21), and t(11;17)(q13;q21) where RARa is fused to the PLZF (promyelocytic leukemia zinc finger), NPM (nucleophosmin) and NuMA (nuclear mitotic apparatus) genes respectively, chromosome 17 and RARa but not PML are involved. Patients were initially repo ...
... In patients with t(11;17)(q23;q21), t(5;17)(q35;q21), and t(11;17)(q13;q21) where RARa is fused to the PLZF (promyelocytic leukemia zinc finger), NPM (nucleophosmin) and NuMA (nuclear mitotic apparatus) genes respectively, chromosome 17 and RARa but not PML are involved. Patients were initially repo ...
Genetic approaches to development: Drosophila as a model organism
... Up-regulation of pgc leads to somatic cellularisation defects similar to the ones observed in torso loss of function alleles Wild-type ...
... Up-regulation of pgc leads to somatic cellularisation defects similar to the ones observed in torso loss of function alleles Wild-type ...
Phenotypic characterization of three temperature
... M o d e s t c h a n g e s in the synthesis o f host a n d E93 v i r a l p r o t e i n s c a n be o b s e r v e d at b o t h t h e p e r m i s s i v e a n d n o n p e r m i s s i v e t e m p e r a t u r e s , (Fig. l c, d). E a r l y p r o t e i n synthesis b e g a n w i t h i n 1 h b u t the shut-of ...
... M o d e s t c h a n g e s in the synthesis o f host a n d E93 v i r a l p r o t e i n s c a n be o b s e r v e d at b o t h t h e p e r m i s s i v e a n d n o n p e r m i s s i v e t e m p e r a t u r e s , (Fig. l c, d). E a r l y p r o t e i n synthesis b e g a n w i t h i n 1 h b u t the shut-of ...
Why Gene Duplication? ,
... fact, the nature of natural selection and chromosomes are such that the incorporation of the multiple copies entails inherent disadvantages. The fact that ribosomal RNA isolated from Xenopus laeuis, of the tailless amphibian order Anura, hybridize very well with the nucleolar organizing DNA isolated ...
... fact, the nature of natural selection and chromosomes are such that the incorporation of the multiple copies entails inherent disadvantages. The fact that ribosomal RNA isolated from Xenopus laeuis, of the tailless amphibian order Anura, hybridize very well with the nucleolar organizing DNA isolated ...
Fatma El-Sayed Ibrahim Ali_A Symmetric Encryption Algorithm
... CYTOSINE(C) and GUANINE (G), which can be encoded based on 0 or 1 by four digits: 0=A(00), 1=C(01), 2=G(10), 3=T(11). This binary coding is used to transfer the binary secret message to DNA sequence. Then from DNA sequence to Amino Acid form. There are 64 possible 3-letter combinations of the DNA co ...
... CYTOSINE(C) and GUANINE (G), which can be encoded based on 0 or 1 by four digits: 0=A(00), 1=C(01), 2=G(10), 3=T(11). This binary coding is used to transfer the binary secret message to DNA sequence. Then from DNA sequence to Amino Acid form. There are 64 possible 3-letter combinations of the DNA co ...
Document
... Fig. 23.9 Chloroplasts are inherited via the seed cytoplasm 3 types of eggs (female): ...
... Fig. 23.9 Chloroplasts are inherited via the seed cytoplasm 3 types of eggs (female): ...
chapter_13b
... Fig. 23.9 Chloroplasts are inherited via the seed cytoplasm 3 types of eggs (female): ...
... Fig. 23.9 Chloroplasts are inherited via the seed cytoplasm 3 types of eggs (female): ...
MICRO-MANIPULATION OF CHICKEN CHROM OSOMES AND
... No, DISC-PCR as the technique has been named, has been recently developed. Even though it allows assignment of small fragments of DNA by PCR amplification directly from chromosomes, it also requires the observation of a very large number of metaphase plates and statistical analysis of signals observ ...
... No, DISC-PCR as the technique has been named, has been recently developed. Even though it allows assignment of small fragments of DNA by PCR amplification directly from chromosomes, it also requires the observation of a very large number of metaphase plates and statistical analysis of signals observ ...
Transcripts of the MHM region on the chicken Z chromosome
... but much less methylated on the single Z chromosome in the female. The state of methylation of the MHM region is established after fertilization by about the 1-day embryonic stage. The MHM region is transcribed only in the female from the particular strand into heterogeneous, high molecular-mass, no ...
... but much less methylated on the single Z chromosome in the female. The state of methylation of the MHM region is established after fertilization by about the 1-day embryonic stage. The MHM region is transcribed only in the female from the particular strand into heterogeneous, high molecular-mass, no ...
Modular proteins I
... Exon shuffling by intronic recombination Middle repetitive sequences flanking an exon may facilitate “looping out” or insertion of modules by intronic recombination Best example of contraction and expansion of a multidomain protein found in apolipoprotein(a): Number of tandem kringle domains ranges ...
... Exon shuffling by intronic recombination Middle repetitive sequences flanking an exon may facilitate “looping out” or insertion of modules by intronic recombination Best example of contraction and expansion of a multidomain protein found in apolipoprotein(a): Number of tandem kringle domains ranges ...
Allele replacement: an application that permits rapid manipulation of
... not accurately quantify the p45–25 packaging efficiency, as in some instances we could detect helper virus that had arisen via recombination between the homologous sequences present in both p45–25 and the amplicon plasmid, that is, OriS. These data suggest that despite the advantage of DNA stability ...
... not accurately quantify the p45–25 packaging efficiency, as in some instances we could detect helper virus that had arisen via recombination between the homologous sequences present in both p45–25 and the amplicon plasmid, that is, OriS. These data suggest that despite the advantage of DNA stability ...
Primary transcript
A primary transcript is the single-stranded ribonucleic acid (RNA) product synthesized by transcription of DNA, and processed to yield various mature RNA products such as mRNAs, tRNAs, and rRNAs. The primary transcripts designated to be mRNAs are modified in preparation for translation. For example, a precursor messenger RNA (pre-mRNA) is a type of primary transcript that becomes a messenger RNA (mRNA) after processing.There are several steps contributing to the production of primary transcripts. All these steps involve a series of interactions to initiate and complete the transcription of DNA in the nucleus of eukaryotes. Certain factors play key roles in the activation and inhibition of transcription, where they regulate primary transcript production. Transcription produces primary transcripts that are further modified by several processes. These processes include the 5' cap, 3'-polyadenylation, and alternative splicing. In particular, alternative splicing directly contributes to the diversity of mRNA found in cells. The modifications of primary transcripts have been further studied in research seeking greater knowledge of the role and significance of these transcripts. Experimental studies based on molecular changes to primary transcripts the processes before and after transcription have led to greater understanding of diseases involving primary transcripts.