cDNA chips
... rich source of basic information and commercial and academic links DNA chips for dummies animation A step by step description of a microarray experiment by Jeremy Buhler The Big Leagues: Pat Brown and NHGRI ...
... rich source of basic information and commercial and academic links DNA chips for dummies animation A step by step description of a microarray experiment by Jeremy Buhler The Big Leagues: Pat Brown and NHGRI ...
X-inactivation
... Xist) due to no inactivation of the Xist deleted X chromosome (129 is B). This leads to skewed monoallelic expression between clones because A (PGK) is always inactivated and no X-related RNA is observed from it. ...
... Xist) due to no inactivation of the Xist deleted X chromosome (129 is B). This leads to skewed monoallelic expression between clones because A (PGK) is always inactivated and no X-related RNA is observed from it. ...
Erythematosus The Epigenetic Face of Systemic Lupus
... Two major groups of changes contribute to defining the epigenome of a cell: DNA methylation and histone modifications. The most common form of DNA methylation occurs at the 5⬘ position of cytosine in the context of CpG dinucleotides, which are unevenly distributed throughout the genome. Particularly ...
... Two major groups of changes contribute to defining the epigenome of a cell: DNA methylation and histone modifications. The most common form of DNA methylation occurs at the 5⬘ position of cytosine in the context of CpG dinucleotides, which are unevenly distributed throughout the genome. Particularly ...
12.3 How Is Biotechnology Used In Forensic Science?
... them a gene, called Bt, from the bacterium, Bacillus thuringiensis. • The protein encoded by the Bt gene damages the _________ tract of insects, but not mammals. • Bt crops therefore suffer less damage from insects, and farmers have to apply less pesticide to their fields. ...
... them a gene, called Bt, from the bacterium, Bacillus thuringiensis. • The protein encoded by the Bt gene damages the _________ tract of insects, but not mammals. • Bt crops therefore suffer less damage from insects, and farmers have to apply less pesticide to their fields. ...
Changes in DNA-binding activity of transcription factors in the
... This study showed that nuclear proteins from the bovine mammary gland form two NFI-specific DNA-protein complexes (Photo 2A). Changes of slowly migrating complex 1 show some similarity to NFI DNA-protein complex in mice. This DNAprotein complex was abundant in virgin and pregnant heifers but sharply ...
... This study showed that nuclear proteins from the bovine mammary gland form two NFI-specific DNA-protein complexes (Photo 2A). Changes of slowly migrating complex 1 show some similarity to NFI DNA-protein complex in mice. This DNAprotein complex was abundant in virgin and pregnant heifers but sharply ...
Detecting phenotype-specific interactions between
... Detecting interactions that have changed significantly in the phenotype • Represent differentially expressed genes, in a phenotype, and their biological functions as a matrix – vector space model with biological processes as column vectors • Find associations between pairs of biological ...
... Detecting interactions that have changed significantly in the phenotype • Represent differentially expressed genes, in a phenotype, and their biological functions as a matrix – vector space model with biological processes as column vectors • Find associations between pairs of biological ...
Minute Sketches - Paul D. Heideman
... copies of each chromosome that are termed homologous (shown by two unconnected chromosomes, with two genes that are marked to indicate differences). DNA uses four nitrogenous bases (represented by A, T, C, and G) in a double stranded helix (represented by the wavy lines), with A binding to T and C b ...
... copies of each chromosome that are termed homologous (shown by two unconnected chromosomes, with two genes that are marked to indicate differences). DNA uses four nitrogenous bases (represented by A, T, C, and G) in a double stranded helix (represented by the wavy lines), with A binding to T and C b ...
Lab 1 genomic DNA
... Three components are necessary for successful nucleic acid extraction: 1) inhibition of nucleases, 2) removal of proteins, and 3) physical separation of the nucleic acid from other cellular components. Nuclease inhibition and removal of proteins are not mutually exclusive and often are accomplished ...
... Three components are necessary for successful nucleic acid extraction: 1) inhibition of nucleases, 2) removal of proteins, and 3) physical separation of the nucleic acid from other cellular components. Nuclease inhibition and removal of proteins are not mutually exclusive and often are accomplished ...
presentation slides - Environmental Health and Safety
... microorganisms that are not known to acquire the trait naturally, if such acquisition could compromise the ability to control disease agents in humans, veterinary medicine, or agriculture... Consideration should be given as to whether the drug resistance trait to be used in the experiment would rend ...
... microorganisms that are not known to acquire the trait naturally, if such acquisition could compromise the ability to control disease agents in humans, veterinary medicine, or agriculture... Consideration should be given as to whether the drug resistance trait to be used in the experiment would rend ...
Tissue- and Development-specific Expression of Multiple
... Isolation of multiple nNOS transcripts with unique 59UTRs. Cloning and sequencing of cDNA by RACE from brain, kidney, heart, intestine, and embryo led to the identification of three different nNOS mRNA species that are designated as nNOSa, nNOSb, and nNOSc. nNOSa was overall the most abundant isofor ...
... Isolation of multiple nNOS transcripts with unique 59UTRs. Cloning and sequencing of cDNA by RACE from brain, kidney, heart, intestine, and embryo led to the identification of three different nNOS mRNA species that are designated as nNOSa, nNOSb, and nNOSc. nNOSa was overall the most abundant isofor ...
Chapter 6 - VU Research Portal
... disorder and primary involvement of myelin, oligodendrocytes or astrocytes may be suspected, it is important to realize that tracts are determined by axons and not by oligodendrocytes or astrocytes, suggesting that the primary problem in LBSL may involve neurons or axons. The cell type specific effe ...
... disorder and primary involvement of myelin, oligodendrocytes or astrocytes may be suspected, it is important to realize that tracts are determined by axons and not by oligodendrocytes or astrocytes, suggesting that the primary problem in LBSL may involve neurons or axons. The cell type specific effe ...
An Interaction-Dependent Model for Transcription Factor Binding
... based on genome wide Chromatin Immunoprecipitation (ChIP) assay, 70% of CREBbound regions do not contain the CGTCA motif in the ~800 bps region [12]. More generally, we have found that for a majority of factors for which genome-wide ChIPchip assay has been performed, the derived PWM does not suffici ...
... based on genome wide Chromatin Immunoprecipitation (ChIP) assay, 70% of CREBbound regions do not contain the CGTCA motif in the ~800 bps region [12]. More generally, we have found that for a majority of factors for which genome-wide ChIPchip assay has been performed, the derived PWM does not suffici ...
Responses to Editor
... expression levels in each experiment that are associated with the cellular compartment by crossreferencing with the localization databases. The row “75%” contains the expression value of the top quartile line, i.e. the 75% line. For instance, 75% transcripts belonging to the cytoplasm in the Holsteg ...
... expression levels in each experiment that are associated with the cellular compartment by crossreferencing with the localization databases. The row “75%” contains the expression value of the top quartile line, i.e. the 75% line. For instance, 75% transcripts belonging to the cytoplasm in the Holsteg ...
genes, pseudogenes, deletions, insertion elements and DNA islands
... the aroG gene through to the beginning of opcB and 90% from the end of the opcB gene to comEA. Thus, large portions of the two regions were strongly homologous within each species and between both species. The Opc protein family The opcA region of Ng strains FA1090 and MS11 contains an opcA-like gen ...
... the aroG gene through to the beginning of opcB and 90% from the end of the opcB gene to comEA. Thus, large portions of the two regions were strongly homologous within each species and between both species. The Opc protein family The opcA region of Ng strains FA1090 and MS11 contains an opcA-like gen ...
TGAC * Sequence Polymorphisms Module
... XVI. Determine the function of a gene Click on the ‘OMIM’ link for the gene Q.40: What is the name of the gene? CDH 13 or Cadherin H 13 Q.41: What is the function of the gene? CDH13 function is not fully understood. CDH13 protein may act as a coreceptor for a signaling receptor through which adipone ...
... XVI. Determine the function of a gene Click on the ‘OMIM’ link for the gene Q.40: What is the name of the gene? CDH 13 or Cadherin H 13 Q.41: What is the function of the gene? CDH13 function is not fully understood. CDH13 protein may act as a coreceptor for a signaling receptor through which adipone ...
The Pax and large Maf families of genes in mammalian eye development Vertebrate eye development is dependent on the coordinated action of thousands of genes. A specific group of over one hundred of regulatory genes is both responsible for ocular cell
... in which a certain group of its specific target genes do not respond properly to the protein levels that are below a threshold value (Cvekl and Tamm, 2004). Haploinsufficiency of the mouse Pax2 gene results in kidney and retinal defects. Similar human PAX2 mutations cause renal‐coloboma syndrome ...
... in which a certain group of its specific target genes do not respond properly to the protein levels that are below a threshold value (Cvekl and Tamm, 2004). Haploinsufficiency of the mouse Pax2 gene results in kidney and retinal defects. Similar human PAX2 mutations cause renal‐coloboma syndrome ...
Year 13 Biology, 2010.
... The role of DNA includes DNA structure and replication, the control of gene expression, protein synthesis, and the determination of phenotype. ...
... The role of DNA includes DNA structure and replication, the control of gene expression, protein synthesis, and the determination of phenotype. ...
Review over DNA, RNA, proteins, viruses, bacteria, DNA technology
... c. Genetic information flows from a sequence of nucleotides in a gene to a sequence of amino acids in a protein. Evidence of student learning is a demonstrated understanding of each of the following: .2. In eukaryotic cells the mRNA transcript undergoes a series of enzyme-regulated modifications. To ...
... c. Genetic information flows from a sequence of nucleotides in a gene to a sequence of amino acids in a protein. Evidence of student learning is a demonstrated understanding of each of the following: .2. In eukaryotic cells the mRNA transcript undergoes a series of enzyme-regulated modifications. To ...
COYOTE BIOTECH MD-Box
... approach compared to standard PCR, where the product of the reaction is detected at its end. Two common methods for the detection of products in quantitative PCR are: (1) non-specific fluorescent dyes that intercalate with any doublestranded DNA, and (2) sequence-specific DNA probes consisting of ol ...
... approach compared to standard PCR, where the product of the reaction is detected at its end. Two common methods for the detection of products in quantitative PCR are: (1) non-specific fluorescent dyes that intercalate with any doublestranded DNA, and (2) sequence-specific DNA probes consisting of ol ...
The role of the tumour suppressor p33ING1b in human neoplasia
... modulate p53 dependent transactivation of the kinase inhibitor p21WAF1” In mice, ING1 has been found to encode two protein products, which share 80–88% homology with human ING1 proteins.8 The bigger protein (p37) acts as a p53 cooperator and hence it behaves as a tumour suppressor. In contrast, the ...
... modulate p53 dependent transactivation of the kinase inhibitor p21WAF1” In mice, ING1 has been found to encode two protein products, which share 80–88% homology with human ING1 proteins.8 The bigger protein (p37) acts as a p53 cooperator and hence it behaves as a tumour suppressor. In contrast, the ...
The Chloroplast-Located Homolog of Bacterial DNA Recombinase
... washed in import buffer, and the radioactive translation products separated by 10% SDS-PAGE, then detected by autoradiography. N-Terminal analysis of the mature RecA-AT protein—Transcription and translation was performed as above, with both 33 SMet and 3H-Leu in the reaction medium. Intact isolated ...
... washed in import buffer, and the radioactive translation products separated by 10% SDS-PAGE, then detected by autoradiography. N-Terminal analysis of the mature RecA-AT protein—Transcription and translation was performed as above, with both 33 SMet and 3H-Leu in the reaction medium. Intact isolated ...
Homology-based cloning and expression analysis of Rf genes
... in PPR motifs is similar to that in TPR, and they also contain two alpha helices, according to which they were predicted to be nucleic acid-binding proteins (Small and Peeters, 2000). Recently, it has been confirmed in some cases that the PPRs are single-strand RNA-binding proteins. For example, Lah ...
... in PPR motifs is similar to that in TPR, and they also contain two alpha helices, according to which they were predicted to be nucleic acid-binding proteins (Small and Peeters, 2000). Recently, it has been confirmed in some cases that the PPRs are single-strand RNA-binding proteins. For example, Lah ...
Plant Telomere Biology
... telomeres was done for the next three decades. Nevertheless, all of the great strides in molecular biology during this time—confirmation of DNA as the genetic material, discovery of DNA structure, elucidation of the mechanisms of semiconservative replication, development of DNA cloning techniques, a ...
... telomeres was done for the next three decades. Nevertheless, all of the great strides in molecular biology during this time—confirmation of DNA as the genetic material, discovery of DNA structure, elucidation of the mechanisms of semiconservative replication, development of DNA cloning techniques, a ...
Combination of Reverse Transcription and Multienzyme Restriction
... tool to detect and identify bacteria, but it cannot distinguish between DNA from viable cells and that from dead cells. In addition to the ethidium monoazide bromide PCR method, reverse-transcription PCR (RT-PCR) can differentiate between viable and dead bacteria because most mRNAs have short half-l ...
... tool to detect and identify bacteria, but it cannot distinguish between DNA from viable cells and that from dead cells. In addition to the ethidium monoazide bromide PCR method, reverse-transcription PCR (RT-PCR) can differentiate between viable and dead bacteria because most mRNAs have short half-l ...
Primary transcript
A primary transcript is the single-stranded ribonucleic acid (RNA) product synthesized by transcription of DNA, and processed to yield various mature RNA products such as mRNAs, tRNAs, and rRNAs. The primary transcripts designated to be mRNAs are modified in preparation for translation. For example, a precursor messenger RNA (pre-mRNA) is a type of primary transcript that becomes a messenger RNA (mRNA) after processing.There are several steps contributing to the production of primary transcripts. All these steps involve a series of interactions to initiate and complete the transcription of DNA in the nucleus of eukaryotes. Certain factors play key roles in the activation and inhibition of transcription, where they regulate primary transcript production. Transcription produces primary transcripts that are further modified by several processes. These processes include the 5' cap, 3'-polyadenylation, and alternative splicing. In particular, alternative splicing directly contributes to the diversity of mRNA found in cells. The modifications of primary transcripts have been further studied in research seeking greater knowledge of the role and significance of these transcripts. Experimental studies based on molecular changes to primary transcripts the processes before and after transcription have led to greater understanding of diseases involving primary transcripts.