Laboratory of Molecular Genetics, KNU
... (10) Mate two heterozygotes and genotype their offspring. This will give all three genotypes--wild type homozygotes, heterozygotes, and transgenic homozygotes. ...
... (10) Mate two heterozygotes and genotype their offspring. This will give all three genotypes--wild type homozygotes, heterozygotes, and transgenic homozygotes. ...
Solid Tumour Section inv(X)(p11.4p11.22) BCOR/CCNB3 in bone sarcoma Atlas of Genetics and Cytogenetics
... NIH3T3 fibroblasts overexpressing BCOR-CCNB3 or ∆CCNB3, a truncated version of CCNB3 corresponding to the portion involved in the fusion gene, was performed. Compared to the empty vector, both expression vectors led to a twofold increase in the proportion of cells in S phase. Although cell cycle ana ...
... NIH3T3 fibroblasts overexpressing BCOR-CCNB3 or ∆CCNB3, a truncated version of CCNB3 corresponding to the portion involved in the fusion gene, was performed. Compared to the empty vector, both expression vectors led to a twofold increase in the proportion of cells in S phase. Although cell cycle ana ...
Cis-regulatory mutations in human disease
... respect to genotype, while feasible in some readily accessible human cell types, remains a significant challenge during human embryonic development. It is therefore common practice to use in vitro assays, or experimental organisms as a proxy. To determine whether the MCS possessed cis-regulatory fun ...
... respect to genotype, while feasible in some readily accessible human cell types, remains a significant challenge during human embryonic development. It is therefore common practice to use in vitro assays, or experimental organisms as a proxy. To determine whether the MCS possessed cis-regulatory fun ...
A twist in sea urchin gastrulation and mesoderm specification
... secondary mesenchyme cells, are specified prior to the initiation of gastrulation [3]. Cell dissociation experiments have substantiated this for the endoderm [4J, but when and how th e different secondary mesenchyme lineages are specified is uncertam. The only secondary mesenchyme lineage for which ...
... secondary mesenchyme cells, are specified prior to the initiation of gastrulation [3]. Cell dissociation experiments have substantiated this for the endoderm [4J, but when and how th e different secondary mesenchyme lineages are specified is uncertam. The only secondary mesenchyme lineage for which ...
Gene Section FANCD2 (Fanconi anemia, complementation group D2) Atlas of Genetics and Cytogenetics
... FANCE, FANCF, and FANCG; this complex is only found in the nucleus. FANCA and FANCG form a complex in the cytoplasm, through a N-term FANCA (involving the nuclear localization signal) - FANCG interaction; FANCC join the complex; phosphorylation of FANCA would induce its translocation into the nucleu ...
... FANCE, FANCF, and FANCG; this complex is only found in the nucleus. FANCA and FANCG form a complex in the cytoplasm, through a N-term FANCA (involving the nuclear localization signal) - FANCG interaction; FANCC join the complex; phosphorylation of FANCA would induce its translocation into the nucleu ...
Non-coding RNAs are widely distributed in the 3 life domains
... CsrA and CsrA homolog RsmA and effect of sequestration by sRNA(A) Gene expression is controlled by CsrA binding to leader segments of target mRNAs (e.g. pgaABCD involved in PGA biosynthesis and export) affecting their translation and stability. CsrA activity is repressed via sequestration of CsrA b ...
... CsrA and CsrA homolog RsmA and effect of sequestration by sRNA(A) Gene expression is controlled by CsrA binding to leader segments of target mRNAs (e.g. pgaABCD involved in PGA biosynthesis and export) affecting their translation and stability. CsrA activity is repressed via sequestration of CsrA b ...
Chapter 10
... DNA replication takes place in large complexes called replication factories, where replication of multiple DNA molecules takes place. ...
... DNA replication takes place in large complexes called replication factories, where replication of multiple DNA molecules takes place. ...
Gene Section STEAP2 (six transmembrane epithelial antigen of the prostate 2)
... STEAP2 is highly expressed in androgen receptorpositive prostate cancer cell line LNCaP, but not in the androgen receptor-negative prostate cancer cell lines PC-3 and DU-145. However, STEAP2 expression is not significantly regulated by androgens in prostate cancer cells (Korkmaz et al., 2002). In hu ...
... STEAP2 is highly expressed in androgen receptorpositive prostate cancer cell line LNCaP, but not in the androgen receptor-negative prostate cancer cell lines PC-3 and DU-145. However, STEAP2 expression is not significantly regulated by androgens in prostate cancer cells (Korkmaz et al., 2002). In hu ...
word
... Name it as sgRNA, give it a “feature type” such as “misc”, choose a color and click OK. Now this sgRNA is added to the list of features. Continue to add all the sgRNA candidates you'd like to keep in your list of features. 8b) Before or after you have searched for CCN19CC sequences in your ApE file ...
... Name it as sgRNA, give it a “feature type” such as “misc”, choose a color and click OK. Now this sgRNA is added to the list of features. Continue to add all the sgRNA candidates you'd like to keep in your list of features. 8b) Before or after you have searched for CCN19CC sequences in your ApE file ...
Supplementary Figure Legends - Word file
... silenced due to the interaction of silencing factors with cis-acting var promoter elements. Activation of the episomal upsC promoter (orange) is only possible if the episome occupies an exclusive var transcription site (position 3) that is associated with the active chromosomeend cluster at the nucl ...
... silenced due to the interaction of silencing factors with cis-acting var promoter elements. Activation of the episomal upsC promoter (orange) is only possible if the episome occupies an exclusive var transcription site (position 3) that is associated with the active chromosomeend cluster at the nucl ...
Case Study Learning via Simulations of Molecular Biology Techniques
... The disease is multifactorial and inheritance patterns are complex. Some forms of familial Alzheimer disease appear to be inherited as autosomal dominant traits, while others are recessive. Spontaneous Alzheimer disease also can occur in the absence of inherited factors. Mutations in at least four g ...
... The disease is multifactorial and inheritance patterns are complex. Some forms of familial Alzheimer disease appear to be inherited as autosomal dominant traits, while others are recessive. Spontaneous Alzheimer disease also can occur in the absence of inherited factors. Mutations in at least four g ...
Nuclear Genes That Encode Mitochondrial Proteins
... sion of particular subgenomic DNA molecules to nearly undetectable levels during plant development. This process, first discovered in maize (Small et al., 1987), appears to be widespread in plants and may constitute a means of maintaining mitochondrial genetic variation in a silenced but retrievable ...
... sion of particular subgenomic DNA molecules to nearly undetectable levels during plant development. This process, first discovered in maize (Small et al., 1987), appears to be widespread in plants and may constitute a means of maintaining mitochondrial genetic variation in a silenced but retrievable ...
POLYMERASE-CHAIN-REACTION (PCR) ANALYSIS OF
... using primers designedto flank the microsatel- radiation from an extra band and a main band. Inheritancepattern.--To investigatethe inherlites gaveamplificationproductsof varying but approximately expectedsize. No individual ex- itancepattern of the avian microsatellites, they hibited more than two ...
... using primers designedto flank the microsatel- radiation from an extra band and a main band. Inheritancepattern.--To investigatethe inherlites gaveamplificationproductsof varying but approximately expectedsize. No individual ex- itancepattern of the avian microsatellites, they hibited more than two ...
guidelines
... tubes, plates, pipette tips, boxes for storing, but also pens and pencils!) should be treated separately from those ones used in other labs! These kinds of products should be autoclaved and/or UV sterilized. ...
... tubes, plates, pipette tips, boxes for storing, but also pens and pencils!) should be treated separately from those ones used in other labs! These kinds of products should be autoclaved and/or UV sterilized. ...
Quantitating Maxwell® Extracted DNA Samples Using the
... concentration. The QuantiFluor® dsDNA System provides a fluorescent DNAbinding dye that enables sensitive and specific quantitation of small amounts of double-stranded DNA (dsDNA) in solution. The dye shows minimal binding to single-stranded DNA (ssDNA) and RNA, allowing specific quantitation of dsD ...
... concentration. The QuantiFluor® dsDNA System provides a fluorescent DNAbinding dye that enables sensitive and specific quantitation of small amounts of double-stranded DNA (dsDNA) in solution. The dye shows minimal binding to single-stranded DNA (ssDNA) and RNA, allowing specific quantitation of dsD ...
Gene Section WT1 (Wilms' tumor suppressor gene) Atlas of Genetics and Cytogenetics
... Nephroblastoma of childhood. Prognosis Good with treatment according to NWTS or SIOP. Cytogenetics 11p13 deletions/translocations can be seen in some cases. Oncogenesis Up to 15% of tumors show mainly biallelic inactivation of WT1 through deletion or mutation. ...
... Nephroblastoma of childhood. Prognosis Good with treatment according to NWTS or SIOP. Cytogenetics 11p13 deletions/translocations can be seen in some cases. Oncogenesis Up to 15% of tumors show mainly biallelic inactivation of WT1 through deletion or mutation. ...
PPT
... • Insertion of CHD7 with subsequent amplification results in increased gene copy number & regulatory elements • OVEREXPRESSION ...
... • Insertion of CHD7 with subsequent amplification results in increased gene copy number & regulatory elements • OVEREXPRESSION ...
GENE EXPRESSION
... gene expression, an Escherichia coli cell, for example, would produce all its proteins in large quantities all the time, and all the cells in a eukaryotic organism would be identical. Although most control mechanisms are negative (preventing something from happening), controls can also be positive ( ...
... gene expression, an Escherichia coli cell, for example, would produce all its proteins in large quantities all the time, and all the cells in a eukaryotic organism would be identical. Although most control mechanisms are negative (preventing something from happening), controls can also be positive ( ...
Chapter 20
... Storing Cloned Genes in DNA Libraries • The cloning procedure just discussed does not target a single gene for cloning. Thousands of different recombinant plasmids are produced in step 3, and a clone of cells carrying each type of plasmid ends up as a white colony in step 5. – A genomic library is ...
... Storing Cloned Genes in DNA Libraries • The cloning procedure just discussed does not target a single gene for cloning. Thousands of different recombinant plasmids are produced in step 3, and a clone of cells carrying each type of plasmid ends up as a white colony in step 5. – A genomic library is ...
Objective 2.1 Lesson D Recombinant Organisms
... Go to DNAi (www.dnai.org) > Manipulation. Focus on the modules Revolution and Production, found at the bottom of the page. Work through the problem, players, pieces of the puzzle and putting it together sections of these modules. Answer on a separate sheet of paper. 1. What is recombinant DNA? 2. Ex ...
... Go to DNAi (www.dnai.org) > Manipulation. Focus on the modules Revolution and Production, found at the bottom of the page. Work through the problem, players, pieces of the puzzle and putting it together sections of these modules. Answer on a separate sheet of paper. 1. What is recombinant DNA? 2. Ex ...
Quantitative analysis to assess the performance of the
... Comparative genomic hybridization (CGH) is a technique for studying chromosomal changes in cancer. As cancerous cells multiply, they can undergo dramatic chromosomal changes, including chromosome loss, duplication, and the translocation of DNA from one chromosome to another. Chromosome aberrations h ...
... Comparative genomic hybridization (CGH) is a technique for studying chromosomal changes in cancer. As cancerous cells multiply, they can undergo dramatic chromosomal changes, including chromosome loss, duplication, and the translocation of DNA from one chromosome to another. Chromosome aberrations h ...
Clox, a mammalian homeobox gene related to Drosophila cut
... mammalian homeoprotein that is closely related to Cut and CDP, named Clox (for Cut-like homeobox). Clox contains the three diagnostic internal cut repeats and a homeodomain that is more closely related to that of Cut and CDP than to other homeoproteins. Clox comprises a family of nuclear proteins th ...
... mammalian homeoprotein that is closely related to Cut and CDP, named Clox (for Cut-like homeobox). Clox contains the three diagnostic internal cut repeats and a homeodomain that is more closely related to that of Cut and CDP than to other homeoproteins. Clox comprises a family of nuclear proteins th ...
Role of microRNA in Skeleton Development
... the miRNA function, this approach often is used as an experimental loss-of-function strategy to ...
... the miRNA function, this approach often is used as an experimental loss-of-function strategy to ...
Primary transcript
A primary transcript is the single-stranded ribonucleic acid (RNA) product synthesized by transcription of DNA, and processed to yield various mature RNA products such as mRNAs, tRNAs, and rRNAs. The primary transcripts designated to be mRNAs are modified in preparation for translation. For example, a precursor messenger RNA (pre-mRNA) is a type of primary transcript that becomes a messenger RNA (mRNA) after processing.There are several steps contributing to the production of primary transcripts. All these steps involve a series of interactions to initiate and complete the transcription of DNA in the nucleus of eukaryotes. Certain factors play key roles in the activation and inhibition of transcription, where they regulate primary transcript production. Transcription produces primary transcripts that are further modified by several processes. These processes include the 5' cap, 3'-polyadenylation, and alternative splicing. In particular, alternative splicing directly contributes to the diversity of mRNA found in cells. The modifications of primary transcripts have been further studied in research seeking greater knowledge of the role and significance of these transcripts. Experimental studies based on molecular changes to primary transcripts the processes before and after transcription have led to greater understanding of diseases involving primary transcripts.