Homogenisation in the ribosomal RNA genes of an Epichloe
... gene arrays occurs more slowly than that of rDNA arrays in hybrids. However the 5S rRNA genes in the Epichloe endophytes were shown to be organised as dispersed copies, not in tandem arrays. Shared polymorphisms between Lp 1 , Lp5 and E8 may indicate the homogenisation rate of these dispersed repeat ...
... gene arrays occurs more slowly than that of rDNA arrays in hybrids. However the 5S rRNA genes in the Epichloe endophytes were shown to be organised as dispersed copies, not in tandem arrays. Shared polymorphisms between Lp 1 , Lp5 and E8 may indicate the homogenisation rate of these dispersed repeat ...
Exam 2
... B. puffer fish show greater genetic variety than E. coli. C. a nematode and flowering mustard plant have the same number of chromosomes. D. the larger the genome of an organism, the greater the number of proteins it produces. Question 18 β-cells in the human pancreas produce insulin. mRNA transcript ...
... B. puffer fish show greater genetic variety than E. coli. C. a nematode and flowering mustard plant have the same number of chromosomes. D. the larger the genome of an organism, the greater the number of proteins it produces. Question 18 β-cells in the human pancreas produce insulin. mRNA transcript ...
Mitochondrial DNA: The Second Genetic System
... length of mitochondrial DNA almost completely. The majority of the genes are transcribed from one of the two strands, which is designated as the heavy (H) strand because of its relative density in a solution of cesium chloride. These genes include those for the 2 rRNA species, 12 genes coding for pr ...
... length of mitochondrial DNA almost completely. The majority of the genes are transcribed from one of the two strands, which is designated as the heavy (H) strand because of its relative density in a solution of cesium chloride. These genes include those for the 2 rRNA species, 12 genes coding for pr ...
Suppression of the Malignant Phenotype in Somatic Cell Hybrids
... well as the detection of the viral DNA in the tumor (6) have provided strong evidence for an etiological role of EBV in the development of endemic BL. In addition specific chromosomal translocations are consist ently found in BL which involve the c-myc gene on chromosome 8 and immunoglobulin gene lo ...
... well as the detection of the viral DNA in the tumor (6) have provided strong evidence for an etiological role of EBV in the development of endemic BL. In addition specific chromosomal translocations are consist ently found in BL which involve the c-myc gene on chromosome 8 and immunoglobulin gene lo ...
Stretching DNA Fibers out of a Chromosome in Solution
... The total length of DNA wound on a chromosome ranges from several hundred micrometer of bacterial or eucaryotic cells to several meter of higher species. Since DNA is only 2-nm thick, it is fragile and easily broken even by a gentle flow of the surrounding medium during handling. The present study a ...
... The total length of DNA wound on a chromosome ranges from several hundred micrometer of bacterial or eucaryotic cells to several meter of higher species. Since DNA is only 2-nm thick, it is fragile and easily broken even by a gentle flow of the surrounding medium during handling. The present study a ...
Chapter 14
... cro, which are transcribed by host RNA polymerase. • N is required to express the delayed early genes. ...
... cro, which are transcribed by host RNA polymerase. • N is required to express the delayed early genes. ...
Drugs and addiction: an introduction to epigenetics
... chromatin structure and small interfering RNA (siRNA) Chromatin existing in an activated open state that permits the access of the cells’ transcriptional machinery to DNA, promoting gene expression Condensed chromatin represented by the tight packaging of the DNA and histone proteins that is associa ...
... chromatin structure and small interfering RNA (siRNA) Chromatin existing in an activated open state that permits the access of the cells’ transcriptional machinery to DNA, promoting gene expression Condensed chromatin represented by the tight packaging of the DNA and histone proteins that is associa ...
Student Guide - the BIOTECH Project
... 1. Get your electrophoresis apparatus and seal both ends of the gel tray with tape or stoppers. 2. Make sure one comb is in place at the negative electrode (black end of the gel). 3. Pour melted agarose into the gel space until the gel is about 5 mm deep. Let the agarose harden, which should take 5- ...
... 1. Get your electrophoresis apparatus and seal both ends of the gel tray with tape or stoppers. 2. Make sure one comb is in place at the negative electrode (black end of the gel). 3. Pour melted agarose into the gel space until the gel is about 5 mm deep. Let the agarose harden, which should take 5- ...
This article appeared in a journal published by Elsevier. The
... CMV strains can be classified into three subgroups, IA, IB and II [20]. It has been found that subgroup I strains are, in general, more virulent than subgroup II strains and some studies have suggested that this differential virulence is mediated by the 2b gene [21]. Alignment of aa sequences of 2b p ...
... CMV strains can be classified into three subgroups, IA, IB and II [20]. It has been found that subgroup I strains are, in general, more virulent than subgroup II strains and some studies have suggested that this differential virulence is mediated by the 2b gene [21]. Alignment of aa sequences of 2b p ...
Biology 261 Molecular and General Genetics
... Welcome to Molecular and General Genetics Genetics is one of the most interesting and important topics in biology. It is a fascinating subject in itself, and in the last few decades it has also become an extremely important component of many other specialities within biology. There have been very ra ...
... Welcome to Molecular and General Genetics Genetics is one of the most interesting and important topics in biology. It is a fascinating subject in itself, and in the last few decades it has also become an extremely important component of many other specialities within biology. There have been very ra ...
SGD sample annotations
... of Rcl1p leads to inhibition of processing at sites A0, A1, and A2”. As cleavage of these three sites within the 35S pre-rRNA is part of processing the primary 35S rRNA transcript, the appropriate process term is “35S primary transcript processing” and the appropriate evidence code is IMP because th ...
... of Rcl1p leads to inhibition of processing at sites A0, A1, and A2”. As cleavage of these three sites within the 35S pre-rRNA is part of processing the primary 35S rRNA transcript, the appropriate process term is “35S primary transcript processing” and the appropriate evidence code is IMP because th ...
H National Quali cations EXEMPLAR PAPER ONLY EP04/H
... prokaryotes. DNA molecules are double-stranded whereas RNA molecules are single-stranded. (a) (i) Give two other structural differences between DNA and RNA molecules. ...
... prokaryotes. DNA molecules are double-stranded whereas RNA molecules are single-stranded. (a) (i) Give two other structural differences between DNA and RNA molecules. ...
Here - EdSpace
... Through plasmid based delivery, 20bp sgRNAs can be integrated into a template plasmid through site-directed mutagenesis. Genomic software can be used to determine the idea location to insert sgRNAs into a plasmid. Construction of an expression plasmid for sgRNA is quite simple and rapid as it only r ...
... Through plasmid based delivery, 20bp sgRNAs can be integrated into a template plasmid through site-directed mutagenesis. Genomic software can be used to determine the idea location to insert sgRNAs into a plasmid. Construction of an expression plasmid for sgRNA is quite simple and rapid as it only r ...
Barbara McClintock
... Discovering science and pursuing an education In high school Barbara discovered science and the joy of problem solving. She decided to pursue science in college. Each of the McClintock children’s individuality had been supported and encouraged — at least until adolescence. Then Barbara’s mother beca ...
... Discovering science and pursuing an education In high school Barbara discovered science and the joy of problem solving. She decided to pursue science in college. Each of the McClintock children’s individuality had been supported and encouraged — at least until adolescence. Then Barbara’s mother beca ...
Full-Text PDF
... through functional analysis [3]. Concomitant with the rapid accumulation of available genetic information, this reverse genetics approach is increasingly used for strain improvement in this synthetic biology age [4]. Specific genes residing in bacterial genomes (or chromosomal DNA) are targeted by v ...
... through functional analysis [3]. Concomitant with the rapid accumulation of available genetic information, this reverse genetics approach is increasingly used for strain improvement in this synthetic biology age [4]. Specific genes residing in bacterial genomes (or chromosomal DNA) are targeted by v ...
7.2mb ppt - UCLA.edu
... cells, which represent a later stage of B-cell differentiation that does not normally undergo SHM. In one hybridoma, mutations were exclusively in GC base pairs that were mostly within RGYW or WRCY motifs, suggesting that AID has primary responsibility for mutations at these nucleotides. The activat ...
... cells, which represent a later stage of B-cell differentiation that does not normally undergo SHM. In one hybridoma, mutations were exclusively in GC base pairs that were mostly within RGYW or WRCY motifs, suggesting that AID has primary responsibility for mutations at these nucleotides. The activat ...
Transcriptional profiling of interleukin-2
... Schematic representation of the RNA-seq experimental design. RNA was isolated from IL-2 treated and non-treated (control) SR and SEN cell culture samples and subject to library preparation and sequencing as described in the Materials and Methods. Panels B-D show the effect of the beta-actin (ACTB) ...
... Schematic representation of the RNA-seq experimental design. RNA was isolated from IL-2 treated and non-treated (control) SR and SEN cell culture samples and subject to library preparation and sequencing as described in the Materials and Methods. Panels B-D show the effect of the beta-actin (ACTB) ...
Coordination of replication and transcription along a Drosophila
... arrays of cDNAs have demonstrated a correlation between time of replication and the probability that a specific gene is expressed, it remained to be determined what step(s) in the replication initiation process are influenced by transcription. Similarly, because the prior studies lacked contiguous i ...
... arrays of cDNAs have demonstrated a correlation between time of replication and the probability that a specific gene is expressed, it remained to be determined what step(s) in the replication initiation process are influenced by transcription. Similarly, because the prior studies lacked contiguous i ...
The Effects of Plasmid on Genotype and Phenotype
... replicate independently of the bacterial chromosome, and many plasmids can also be transferred naturally among their bacterial hosts. Generally, plasmid genes code for proteins that are not essential for the survival of their host in its normal environment. However, some plasmid DNAs encode genes th ...
... replicate independently of the bacterial chromosome, and many plasmids can also be transferred naturally among their bacterial hosts. Generally, plasmid genes code for proteins that are not essential for the survival of their host in its normal environment. However, some plasmid DNAs encode genes th ...
Chromothripsis: how does such a catastrophic event impact human
... since only balanced chromothripsis outcomes compatible with life have been found in individuals to date. Altogether, these key features define the molecular signature of chromothripsis (Maher and Wilson, 2012; Korbel and Campbell, 2013). The definition of these features characterizing chromothripsis i ...
... since only balanced chromothripsis outcomes compatible with life have been found in individuals to date. Altogether, these key features define the molecular signature of chromothripsis (Maher and Wilson, 2012; Korbel and Campbell, 2013). The definition of these features characterizing chromothripsis i ...
Bio 211 Genetics Laboratory Experiment 5: Bioinformatics
... b. The second section, titled Descriptions, lists sequences with significant alignments and information links about each. Clicking on a Max. score number for an Accession item (such as AY258957.1) will link you to the third section. Clicking on the Accession link will take you to a page of in ...
... b. The second section, titled Descriptions, lists sequences with significant alignments and information links about each. Clicking on a Max. score number for an Accession item (such as AY258957.1) will link you to the third section. Clicking on the Accession link will take you to a page of in ...
Primary transcript
A primary transcript is the single-stranded ribonucleic acid (RNA) product synthesized by transcription of DNA, and processed to yield various mature RNA products such as mRNAs, tRNAs, and rRNAs. The primary transcripts designated to be mRNAs are modified in preparation for translation. For example, a precursor messenger RNA (pre-mRNA) is a type of primary transcript that becomes a messenger RNA (mRNA) after processing.There are several steps contributing to the production of primary transcripts. All these steps involve a series of interactions to initiate and complete the transcription of DNA in the nucleus of eukaryotes. Certain factors play key roles in the activation and inhibition of transcription, where they regulate primary transcript production. Transcription produces primary transcripts that are further modified by several processes. These processes include the 5' cap, 3'-polyadenylation, and alternative splicing. In particular, alternative splicing directly contributes to the diversity of mRNA found in cells. The modifications of primary transcripts have been further studied in research seeking greater knowledge of the role and significance of these transcripts. Experimental studies based on molecular changes to primary transcripts the processes before and after transcription have led to greater understanding of diseases involving primary transcripts.