An Experimental Program for Introducing First
... more scientific questions. However, for first and second year biology classes to successfully integrate scientific literature, the right approach must be found through assessing the successes and shortcomings of experimental programs. The program discussed in this paper uses a novel method for educa ...
... more scientific questions. However, for first and second year biology classes to successfully integrate scientific literature, the right approach must be found through assessing the successes and shortcomings of experimental programs. The program discussed in this paper uses a novel method for educa ...
Using High-Throughput Sequencing to Investigate the Transgenerational
... chromatin immunoprecipitation combined with high-throughput sequencing, known as ChIP-Seq. ChIPSeq is used to analyze the binding-site interactions between protein transcription factors and DNA, in particular, the relationship between the specific binding site of those proteins and relative gene exp ...
... chromatin immunoprecipitation combined with high-throughput sequencing, known as ChIP-Seq. ChIPSeq is used to analyze the binding-site interactions between protein transcription factors and DNA, in particular, the relationship between the specific binding site of those proteins and relative gene exp ...
Gene overexpression reveals alternative
... bMin is S. cerevisiae minimal medium that does not induce a response of the general control of amino-acid biosynthesis. 3-AT is Min supplemented with 10 m M 3-amino-l,2,4-triazole, which simulates amino-acid limitation. Cwt is 1eu2-112, ura3-52 $288C derivative complemented with plasmid pRS315[GCN4- ...
... bMin is S. cerevisiae minimal medium that does not induce a response of the general control of amino-acid biosynthesis. 3-AT is Min supplemented with 10 m M 3-amino-l,2,4-triazole, which simulates amino-acid limitation. Cwt is 1eu2-112, ura3-52 $288C derivative complemented with plasmid pRS315[GCN4- ...
Drosophila Past1 is involved in endocytosis and is required for
... Endocytosis in eukaryotes mediates a variety of key cellular processes, such as maintenance of homeostasis, development, uptake of nutrients and downregulation of signal transduction (Le Roy and Wrana, 2005; Mukherjee et al., 1997), which is highly regulated by a complex network of interacting prote ...
... Endocytosis in eukaryotes mediates a variety of key cellular processes, such as maintenance of homeostasis, development, uptake of nutrients and downregulation of signal transduction (Le Roy and Wrana, 2005; Mukherjee et al., 1997), which is highly regulated by a complex network of interacting prote ...
causes2 - Families Against Cancer & Toxics
... • Think of DNA as a zipper, and picture our cells as having tiny scavenger proteins that spend all of their time searching for and eliminating stray threads and foreign matter and broken teeth that have made their way into our DNA zippers. • There is a high incidence of leukemia in children with inh ...
... • Think of DNA as a zipper, and picture our cells as having tiny scavenger proteins that spend all of their time searching for and eliminating stray threads and foreign matter and broken teeth that have made their way into our DNA zippers. • There is a high incidence of leukemia in children with inh ...
Corchorus yellow vein virus, a New World geminivirus from the Old
... iterons, both upstream of the AC1 TATA box, as well as identical stem–loop sequences that included the conserved TAATATTAC nonanucleotide sequence present in the CRs of all characterized geminiviruses (Fig. 1). A PWRLMAGT motif was identified at the N terminus of the deduced CoYVV CP sequence encode ...
... iterons, both upstream of the AC1 TATA box, as well as identical stem–loop sequences that included the conserved TAATATTAC nonanucleotide sequence present in the CRs of all characterized geminiviruses (Fig. 1). A PWRLMAGT motif was identified at the N terminus of the deduced CoYVV CP sequence encode ...
PowerPoint Presentation - Etiology of childhood leukemia
... • Think of DNA as a zipper, and picture our cells as having tiny scavenger proteins that spend all of their time searching for and eliminating stray threads and foreign matter and broken teeth that have made their way into our DNA zippers. • There is a high incidence of leukemia in children with inh ...
... • Think of DNA as a zipper, and picture our cells as having tiny scavenger proteins that spend all of their time searching for and eliminating stray threads and foreign matter and broken teeth that have made their way into our DNA zippers. • There is a high incidence of leukemia in children with inh ...
Protective action of vitamin C against DNA damage induced by
... 37°C. Vitamin C at 10 and 50 mM diminished the extent of DNA damage evoked by (NH3)2Pt(SeO3) but had no effect on the kinetics of DNA repair. The vitamin did not directly inactivate the conjugate. Lymphocytes treated with endonuclease III, which recognises oxidised pyrimidines, displayed a greater t ...
... 37°C. Vitamin C at 10 and 50 mM diminished the extent of DNA damage evoked by (NH3)2Pt(SeO3) but had no effect on the kinetics of DNA repair. The vitamin did not directly inactivate the conjugate. Lymphocytes treated with endonuclease III, which recognises oxidised pyrimidines, displayed a greater t ...
Slides - Workforce Development in Stem Cell Research
... Lecture notes (hyperlink) Activity notes (hyperlink) More links… ...
... Lecture notes (hyperlink) Activity notes (hyperlink) More links… ...
High-Throughput Neurotechnology
... Fig. 4. Schematic of zebrafish manipulation and imaging platform. Larvae are automatically loaded to the system from either reservoirs or multiwell plates. Reservoirs are connected to the system via fluidic valves and a bubble mixer prevents the larvae from settling. The multiwell plate is located o ...
... Fig. 4. Schematic of zebrafish manipulation and imaging platform. Larvae are automatically loaded to the system from either reservoirs or multiwell plates. Reservoirs are connected to the system via fluidic valves and a bubble mixer prevents the larvae from settling. The multiwell plate is located o ...
Bacteriophage-mediated nucleic acid immunisation
... Compared to standard naked DNA technology, the system o¡ers several advantages: vaccine nucleic acid is contained within a stable matrix which appears to both target APCs and protect it from nuclease degradation. Expression constructs of up to 15^20 kb in size can be used in a standard bacteriophage ...
... Compared to standard naked DNA technology, the system o¡ers several advantages: vaccine nucleic acid is contained within a stable matrix which appears to both target APCs and protect it from nuclease degradation. Expression constructs of up to 15^20 kb in size can be used in a standard bacteriophage ...
Read the Nobel Lecture
... First we needed an assay – a way to detect if telomere elongation was happening. The first assay we tried explored whether a piece of DNA that included a telomere would incorporate DNA precursors more readily than a piece of DNA containing non-telomeric sequences. The idea was that if there was an ...
... First we needed an assay – a way to detect if telomere elongation was happening. The first assay we tried explored whether a piece of DNA that included a telomere would incorporate DNA precursors more readily than a piece of DNA containing non-telomeric sequences. The idea was that if there was an ...
Mitosis in Drosophila development - Journal of Cell Science
... correct completion of S phase in order for both nuclear and cytoplasmic events of M phase to take place. This is not to say that some critical aspect of S phase is not completed, and if indeed aphidicolin has its only primary effects on DNA polymerases a and <5, this could well be possible. Neverthe ...
... correct completion of S phase in order for both nuclear and cytoplasmic events of M phase to take place. This is not to say that some critical aspect of S phase is not completed, and if indeed aphidicolin has its only primary effects on DNA polymerases a and <5, this could well be possible. Neverthe ...
Supplementary Information (doc 7548K)
... cord blood cells collected for RNA-seq are shown. Sex and volume of blood obtained from each donor are indicated along with total mononuclear cell (MNC) counts. Percentages of CD34+ cells pre and post FACS sorting are indicated. Supplementary Table 3 | Occurrence of allele-specific expression in non ...
... cord blood cells collected for RNA-seq are shown. Sex and volume of blood obtained from each donor are indicated along with total mononuclear cell (MNC) counts. Percentages of CD34+ cells pre and post FACS sorting are indicated. Supplementary Table 3 | Occurrence of allele-specific expression in non ...
Cold-induced silencing by long antisense transcripts of an
... of a linked reporter. In the context of endogenous FLC silencing we propose that COOLAIR is involved in early, cold-dependent and transient, transcriptional silencing of FLC. This silencing would then be reinforced and epigenetically maintained by the Polycomb machinery. A possible sequence of event ...
... of a linked reporter. In the context of endogenous FLC silencing we propose that COOLAIR is involved in early, cold-dependent and transient, transcriptional silencing of FLC. This silencing would then be reinforced and epigenetically maintained by the Polycomb machinery. A possible sequence of event ...
Identification of genes altered in a mos1 mutagenesis I
... 4. Heat-shock the transformation mixture at 42°C for 45 seconds. 5. Incubate the transformation mixture on ice for 2 minutes. 6. Add 250 μl of pre-warmed LB medium to the transformation reaction mixture. Allow the competent cells to recover for at least 1 hour at 37°C with agitation. (Lay the tube o ...
... 4. Heat-shock the transformation mixture at 42°C for 45 seconds. 5. Incubate the transformation mixture on ice for 2 minutes. 6. Add 250 μl of pre-warmed LB medium to the transformation reaction mixture. Allow the competent cells to recover for at least 1 hour at 37°C with agitation. (Lay the tube o ...
Genes: Structure, Replication, and Mutation
... information on plasmids and the nature of genetic recombination in microorganisms. These three chapters provide the background needed for understanding the material in Part Five: recombinant DNA technology (chapter 14) and microbial genomics (chapter 15). Geneticists, including microbial geneticists ...
... information on plasmids and the nature of genetic recombination in microorganisms. These three chapters provide the background needed for understanding the material in Part Five: recombinant DNA technology (chapter 14) and microbial genomics (chapter 15). Geneticists, including microbial geneticists ...
Lab 6: Electrophoresis
... Restriction endonucleases recognize specific DNA sequences in the double-stranded DNA and digest the DNA at the sites. The result is the production of fragments of DNA of various lengths corresponding to the distance between identical DNA sequences within the chromosome. Some restriction enzymes cut ...
... Restriction endonucleases recognize specific DNA sequences in the double-stranded DNA and digest the DNA at the sites. The result is the production of fragments of DNA of various lengths corresponding to the distance between identical DNA sequences within the chromosome. Some restriction enzymes cut ...
pdf
... periods, labeled nucleotides can be incorporated during initiation of the short nascent chain as well as the during the elongation and termination. Since the 5’ end was labeled only during longer pulses, it must be the part synthesized first. Thus the direction of chain growth is 5’ to 3. Answer 5.1 ...
... periods, labeled nucleotides can be incorporated during initiation of the short nascent chain as well as the during the elongation and termination. Since the 5’ end was labeled only during longer pulses, it must be the part synthesized first. Thus the direction of chain growth is 5’ to 3. Answer 5.1 ...
1. Telomeres 2. Centromeric Repeats 3. Retrotransposons (Class I
... Three of the Tos family (Tos10, Tos17, Tos19) have been shown to be active under tissue culture conditions Tos17 was found to only have two copies in the Nipponbare genome Tos17, when activated, has a preference for insertion into low copy sequences in the rice genome Tos17 activation leads to a gra ...
... Three of the Tos family (Tos10, Tos17, Tos19) have been shown to be active under tissue culture conditions Tos17 was found to only have two copies in the Nipponbare genome Tos17, when activated, has a preference for insertion into low copy sequences in the rice genome Tos17 activation leads to a gra ...
Primary transcript
A primary transcript is the single-stranded ribonucleic acid (RNA) product synthesized by transcription of DNA, and processed to yield various mature RNA products such as mRNAs, tRNAs, and rRNAs. The primary transcripts designated to be mRNAs are modified in preparation for translation. For example, a precursor messenger RNA (pre-mRNA) is a type of primary transcript that becomes a messenger RNA (mRNA) after processing.There are several steps contributing to the production of primary transcripts. All these steps involve a series of interactions to initiate and complete the transcription of DNA in the nucleus of eukaryotes. Certain factors play key roles in the activation and inhibition of transcription, where they regulate primary transcript production. Transcription produces primary transcripts that are further modified by several processes. These processes include the 5' cap, 3'-polyadenylation, and alternative splicing. In particular, alternative splicing directly contributes to the diversity of mRNA found in cells. The modifications of primary transcripts have been further studied in research seeking greater knowledge of the role and significance of these transcripts. Experimental studies based on molecular changes to primary transcripts the processes before and after transcription have led to greater understanding of diseases involving primary transcripts.