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(type I) and mannose-resistant F8 (P) fimbriae of Escherichia coli
(type I) and mannose-resistant F8 (P) fimbriae of Escherichia coli

... shown in this paper the genetic determinants of F8 fimbriae were m a p p e d at a position between 17--20 on the chromosome of strain 2980 of E. coli O18:K5. Therefore, it seems that the gene clusters coding for type I fimbriae are located at fixed positions on the chromosomes of different strains. ...
temperature effects, and localization of a mobile genetic element Dm
temperature effects, and localization of a mobile genetic element Dm

... then a high incidence of pupal mortality would be expected. This was not observed. As regards selection of germ-line cells at the sensitive periods, modifier genes of the radial vein are then nonfunctional, and so their combinations could not be estimated by selection either of this character, or of ...
Standard for the presentation of nucleotide and amino acid
Standard for the presentation of nucleotide and amino acid

... signal sequences, when present, may have negative numbers, counting backwards starting with the amino acid next to number 1. Zero (0) is not used when the numbering of amino acids uses negative numbers to distinguish the mature protein. It shall be marked under the sequence every five amino acids. T ...
The Response of the Phosphate Uptake System and the Organic
The Response of the Phosphate Uptake System and the Organic

... al. 1991, Oshima et al. 1996, Lenburg and O’Shea 1996). Recent studies have shown that Pi starvation in plants results in the activation of multiple genes, suggesting that plants may have a gene regulation system similar to the yeast pho-regulon. In this study, we compared the regulation of the Pi u ...
The development of apical embryonic pattern in Arabidopsis
The development of apical embryonic pattern in Arabidopsis

... must derive from the EPR. In agreement with this, the bases of of embryogenesis, expression has spread and is found in a the cotyledons meet along a region that is three to four cells in continuous band between the presumptive cotyledons (Fig. diameter in the wild-type – approximately the same numbe ...
Saccharomyces cerevisiae.
Saccharomyces cerevisiae.

... that can survive under both conditions. The question is what the difference is between these organisms. Why can some grow only in the presence of molecular oxygen, some only in the absence, and are some able to withstand both conditions? The yeast Saccharomyces cerevisiae is one of the few yeasts wi ...
Practice exam 3 key
Practice exam 3 key

... b) EcoRI cuts both strands of DNA. The position of the first cut is indicated by the arrow above. Draw an arrow to indicate the position of the second cut. (1 pt) See 1a. c) The position of the two cuts makes EcoRI a particularly useful tool for manipulating DNA. Explain. (3 pts) Leaves single-stran ...
Metabolomics based gene function annotation in Escherichia coli
Metabolomics based gene function annotation in Escherichia coli

... mutants. Additionally, metabolomics can be used to explore metabolic diversity of different accessions/strains, tissues, and cell types of an organism. Once the metabolic diversity has been described, the genetic-basis for the metabolic and/or phenotypic differences can be elucidated using other omi ...
Genomic Analysis of Arabidopsis Thaliana
Genomic Analysis of Arabidopsis Thaliana

... Arabidopsis, while millions of clones must be screened for plant species with a huge genome such as wheat and pea. For Southern blotting, less than 1 microgram of DNA is sufficient to detect a singlecopy sequence in Arabidopsis, making it easy to perform RFLP (restriction fragment length polymorphis ...
Developing a Bioassay for Triclosan Toxicity Abstract
Developing a Bioassay for Triclosan Toxicity Abstract

... expression of actin2b, the reference gene, dramatically decreased. This suggests that differences in gene expression of ndr2 and pkd2 between control and TCS treated embryos was not due to differential amounts of the RNA being used for PCR. In order to fully understand the genetic changes, the exper ...
Chapter 14: MENDEL AND THE GENE IDEA
Chapter 14: MENDEL AND THE GENE IDEA

... It also refers to plants that produce offspring of the same variety when they self-pollinate (the offspring are like the parent plants and like each other). For example, a plant with purple flowers is true-breeding if the seeds produced by self-pollination all give rise to plants that also have pu ...
Requirement for chitin biosynthesis in epithelial tube morphogenesis. Proc. Natl. Acad. Sci. USA 102: 17014-17019. pdf
Requirement for chitin biosynthesis in epithelial tube morphogenesis. Proc. Natl. Acad. Sci. USA 102: 17014-17019. pdf

... UAS-GFP transgenes to label tracheal cells with GFP. White dots outline lumen. (F–J) Same as A–E, except lumen is directly visualized by mAb 2A12 staining of similar stage embryos. Because 2A12 antigen is reduced in stronger cystic alleles, histochemical staining reactions for animals in H–J were ex ...
Allelic Variation at the Rht8 Locus in a 19th
Allelic Variation at the Rht8 Locus in a 19th

... Rht8 and associated WMS261-192 bp was introduced from the Japanese variety “Akakomugi” through Italian breeding programs in the 1920’s [4]. After that, it was used in several crossings and spread to the rest of the world [17]. In southern and central Europe, this allele is now very abundant [4, 6] a ...
Role of Genes - Native American Cancer Research
Role of Genes - Native American Cancer Research

... p53 is a tumor suppressor Unless it is damaged (mutation) Bemis, UMN-Duluth, Burhansstipanov, Native American Cancer Initiatives, Incorporated (NACI) Mayo Clinic’s “Spirit of EAGLES Community Network Programs 2” [P.I. Kaur; U54CA153605] ; Northwest Portland Area Indian Health Board; ...
hag expression in Bacillus subtilis is both negatively
hag expression in Bacillus subtilis is both negatively

... (Caldwell et al., 2001), which are mainly transcribed by the sD-dependent RNA polymerase (Ordal et al., 1993). This conclusion has been derived from the transcription profiling of scoC mutant cells (scoC4), which showed that most of the motility genes, including hag, are transcribed at lower levels ...
PPT
PPT

... 17 (c) Mark Gerstein, 2000, Yale, bioinfo.mbb.yale.edu ...
Section 2: Mendel`s Theory
Section 2: Mendel`s Theory

... Mendel’s Second Experiments • A dihybrid cross involves two characters, such as seed color and seed shape. • Mendel used dihybrid crosses in his second experiments and found that the inheritance of one character did not affect the inheritance of another character. • In modern terms, the law of indep ...
Proof-of-principle rapid noninvasive prenatal diagnosis
Proof-of-principle rapid noninvasive prenatal diagnosis

... by the HapMap Consortium (18). Moreover, previous studies have identified a conserved AJ N370S founder haplotype that extends even further downstream of GBA (12, 19). Nevertheless, although it is likely that the founder haplotype is longer than we initially mapped, our SNP-sequencing panel still suc ...
Cancer Prone Disease Section Familial Juvenile Polyposis Syndrome in Oncology and Haematology
Cancer Prone Disease Section Familial Juvenile Polyposis Syndrome in Oncology and Haematology

... presence of five or more juvenile polyps in the colorectum, or at least one upper and one lower GI juvenile polyp, or any number of juvenile polyps in a patient with a family history of Juvenile Polyposis. Juvenile Polyposis has variable expressivity. Even within the same family carrying a particula ...
88KB
88KB

... product was purified under native conditions from the precursor and the cleaved leader sequence (Figure 2b). To ensure all of the 13C,15N-labeled 3′ strand was involved in complex formation, the NMR samples were prepared with excess 5′ strand, and the self-cleaved complex was purified as described a ...
Plant and Soil.
Plant and Soil.

... New molecular approaches that analyze the bacterial genome are renewing our interest in bacterial systematics and taxonomy, and broadening the perception that man has of microbes. These approaches have not only revealed unsuspected relationships among apparently unrelated bacteria, but also demonstr ...
Parallel Genetic and Phenotypic Evolution of DNA Superhelicity in
Parallel Genetic and Phenotypic Evolution of DNA Superhelicity in

... Losos et al. 1998; Huey et al. 2000). Parallel changes sometimes extend even to the genome, as has been described for some viral and bacterial pathogens (Crandall et al. 1999; Reid et al. 2000). The parallel evolution of DNA superhelicity in the long-term experiment with E. coli provides therefore h ...
Genetics of host response in leprosy
Genetics of host response in leprosy

... account for the major clinical differences observed among patients; (ii) epidemiological studies have been reporting consistently that contacts exhibit the highest risk of developing the disease (household contacts with close consanguineous relationship to the index case are at the highest risk);2 ( ...
Applet for calculating heritability for threshold traits
Applet for calculating heritability for threshold traits

... the milk yield. For each A or B allele an individual has a yield increase of one kilogram. The alleles A and a have the same frequency in the distributions. For a realistic picture of the genetic background for milk yields, hundreds of gene pairs have to be involved. The milk yield has by selection ...
clValid: An R Package for Cluster Validation
clValid: An R Package for Cluster Validation

... researcher conducting the experiment. An additional, related problem is determining the number of clusters that are most appropriate for the data. Ideally, the resulting clusters should not only have good statistical properties (compact, well-separated, connected, and stable), but also give results ...
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RNA-Seq



RNA-seq (RNA sequencing), also called whole transcriptome shotgun sequencing (WTSS), is a technology that uses the capabilities of next-generation sequencing to reveal a snapshot of RNA presence and quantity from a genome at a given moment in time.
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