Phytic Acid and Inorganic Phosphate Composition in Soybean Lines
... Reaction conditions were as above with thermocycling parameters: 95°C for 3 minutes followed by 45 cycles of 95°C for 20 seconds, 56°C for 20 seconds, and 72°C for 20 seconds. A melt curve was performed by reading every 0.2°C for 1 second from 52°C to 77° The wild-type (FN38) peak was present at 65° ...
... Reaction conditions were as above with thermocycling parameters: 95°C for 3 minutes followed by 45 cycles of 95°C for 20 seconds, 56°C for 20 seconds, and 72°C for 20 seconds. A melt curve was performed by reading every 0.2°C for 1 second from 52°C to 77° The wild-type (FN38) peak was present at 65° ...
Bonus, a Drosophila TIF1 homologue, is a chromatin
... performed several controls. First, we used an antibody that recognizes the T7–Tag. Drosophila does not contain any proteins with this sequence and therefore we expect no ...
... performed several controls. First, we used an antibody that recognizes the T7–Tag. Drosophila does not contain any proteins with this sequence and therefore we expect no ...
Detection of mcr-1 colistin resistance gene in
... [14]. Given the discrepancies between the international committees and the lack of colistin breakpoints for Enterobacteriaceae in CLSI, we considered it convenient to apply a screening method. Although disc diffusion is not recommended to test colistin susceptibility, it was useful for an initial sc ...
... [14]. Given the discrepancies between the international committees and the lack of colistin breakpoints for Enterobacteriaceae in CLSI, we considered it convenient to apply a screening method. Although disc diffusion is not recommended to test colistin susceptibility, it was useful for an initial sc ...
Structure-Function Analysis of the Conserved Histone Chaperone
... Chromatin structure is crucial to regulate access to the genome for processes such as transcription, recombination, DNA repair, and DNA replication. Spt6, a key factor involved in regulating chromatin struct ...
... Chromatin structure is crucial to regulate access to the genome for processes such as transcription, recombination, DNA repair, and DNA replication. Spt6, a key factor involved in regulating chromatin struct ...
Storage cells in the bone marrow
... What should we do? • We should follow the WHO classification but should note the presence of relevant cytogenetic abnormalities • We should recognize that topoisomerase IIinhibitor-related secondary AML is quite a different matter from t-AML related to alkylating ...
... What should we do? • We should follow the WHO classification but should note the presence of relevant cytogenetic abnormalities • We should recognize that topoisomerase IIinhibitor-related secondary AML is quite a different matter from t-AML related to alkylating ...
PDF - FEMS Microbiology Letters
... and, in an attempt to discover whether both compounds act as substrates for the same enzyme or whether a separate enzyme is involved, he concluded that it seems highly probable that only one enzyme is involved in both decarboxylations (Epps, 1944). Finally in 1948, McGilvery and Cohen, in the course ...
... and, in an attempt to discover whether both compounds act as substrates for the same enzyme or whether a separate enzyme is involved, he concluded that it seems highly probable that only one enzyme is involved in both decarboxylations (Epps, 1944). Finally in 1948, McGilvery and Cohen, in the course ...
Assessment of the mosaic structure in the
... amplicons are sequenced using a battery of gene specific primers (often the PCR primers). In a recent study, we described an improved strategy using M13 and T7 sequence tagged primers in PCR amplification covering the cagA EPIYA motifs. Tagging of the PCR primers enables rapid sequencing using unive ...
... amplicons are sequenced using a battery of gene specific primers (often the PCR primers). In a recent study, we described an improved strategy using M13 and T7 sequence tagged primers in PCR amplification covering the cagA EPIYA motifs. Tagging of the PCR primers enables rapid sequencing using unive ...
Gene Section LMO2 (LIM domain only 2 (rhombotin-like 1))
... Hematopoiesis: LMO2 directly interacts with the basicloop-helix protein TAL1/SCL and the GATA DNA protein GATA1. They form a transcriptional complex: LMO2 has no direct evidence in DNA binding capacity but could act as a bridging molecule bringing together different DNA binding factors (TAL1, LDB1, ...
... Hematopoiesis: LMO2 directly interacts with the basicloop-helix protein TAL1/SCL and the GATA DNA protein GATA1. They form a transcriptional complex: LMO2 has no direct evidence in DNA binding capacity but could act as a bridging molecule bringing together different DNA binding factors (TAL1, LDB1, ...
factor involved in dorsal-ventral axis formation and neurogenesis
... that fragment. MNR31, MNR62, cl-7, and MNR73 all contain exon 5 from the 1.3-kb EcoRI fragment and, thus, differ only in the 5'-most exon. MNR22 begins at the same site as MNR73 but reads through the splice signals utilized by MNR73. The final cDNA examined, c3-0, appears to be a partial unprocessed ...
... that fragment. MNR31, MNR62, cl-7, and MNR73 all contain exon 5 from the 1.3-kb EcoRI fragment and, thus, differ only in the 5'-most exon. MNR22 begins at the same site as MNR73 but reads through the splice signals utilized by MNR73. The final cDNA examined, c3-0, appears to be a partial unprocessed ...
Use of GenoType MTBDR plus assay for the detection of
... does not lose its enzymatic activity. The mycobacterial cells, having a inhA gene mutation synthesis of mycolic acids, occurs in the presence of isoniazid. It should, however, be noted that commercial molecular tests only take into account those areas of the genes responsible for drug resistance in ...
... does not lose its enzymatic activity. The mycobacterial cells, having a inhA gene mutation synthesis of mycolic acids, occurs in the presence of isoniazid. It should, however, be noted that commercial molecular tests only take into account those areas of the genes responsible for drug resistance in ...
Biochemical but not clinical vitamin A deficiency results from
... normal. In addition, other factors associated with low retinol concentrations (eg, low plasma transthyretin or zinc concentrations or mutations in the transthyretin gene) were not present. Neither sibling had a history of systemic disease. Objective: Our aim was to investigate the cause of the retin ...
... normal. In addition, other factors associated with low retinol concentrations (eg, low plasma transthyretin or zinc concentrations or mutations in the transthyretin gene) were not present. Neither sibling had a history of systemic disease. Objective: Our aim was to investigate the cause of the retin ...
GAlibLecture
... main continued // Now create the GA and run it. First we create a genome of the type that // we want to use in the GA. The ga doesn't operate on this genome in the // optimization - it just uses it to clone a population of genomes. //Create the genome object GA1DArrayAlleleGenome genome(leng, ...
... main continued // Now create the GA and run it. First we create a genome of the type that // we want to use in the GA. The ga doesn't operate on this genome in the // optimization - it just uses it to clone a population of genomes. //Create the genome object GA1DArrayAlleleGenome
Mutational effects depend on ploidy level: all else is not equal
... A factor that has been left out of this (and similar) discussions is whether all else is equal between individuals of different ploidy. Although direct empirical tests are relatively sparse, the existing data suggest that ploidy background may well affect the properties of mutations. The effect size ...
... A factor that has been left out of this (and similar) discussions is whether all else is equal between individuals of different ploidy. Although direct empirical tests are relatively sparse, the existing data suggest that ploidy background may well affect the properties of mutations. The effect size ...
fulltext - DiVA portal
... and SheA) which causes lysis of mammalian cells by pore formation in a calcium-independent fashion. ClyA is the only cytolytic factor found in non-pathogenic strains of E. coli including the K-12 strains commonly used in laboratory studies [2-7]. The clyA+ transcription is known to be subjected to t ...
... and SheA) which causes lysis of mammalian cells by pore formation in a calcium-independent fashion. ClyA is the only cytolytic factor found in non-pathogenic strains of E. coli including the K-12 strains commonly used in laboratory studies [2-7]. The clyA+ transcription is known to be subjected to t ...
ARTICLE Functional analysis of mutations in SLC7A9, and genotype
... (Table 1). This is consistent with a previous study on the same subgroup of Libyan Jewish cystinuria families (32). Structure of the SLC7A9 gene After screening the RPCI-5 PAC library using an SLC7A9 cDNA probe, two clones were selected to determine the genomic structure of the SLC7A9 gene: clone 10 ...
... (Table 1). This is consistent with a previous study on the same subgroup of Libyan Jewish cystinuria families (32). Structure of the SLC7A9 gene After screening the RPCI-5 PAC library using an SLC7A9 cDNA probe, two clones were selected to determine the genomic structure of the SLC7A9 gene: clone 10 ...
CH # 13-3
... Sickle cell disease is a disorder associated with changes in the shape of red blood cells. Normal red blood cells are round. Sickle cells appear long and pointed. Sickle cell disease is caused by a point mutation in one of the polypeptides found in hemoglobin, the blood’s principal oxygencarrying pr ...
... Sickle cell disease is a disorder associated with changes in the shape of red blood cells. Normal red blood cells are round. Sickle cells appear long and pointed. Sickle cell disease is caused by a point mutation in one of the polypeptides found in hemoglobin, the blood’s principal oxygencarrying pr ...
Identifying Hepatic Nuclear Factor 1 Mutations in
... the increased risk of diabetes in their relatives. It is impractical to screen all patients with type 1 diabetes for HNF-1␣ mutations, and a selective approach to identify individuals for HNF-1␣ sequencing is required. We used family history as the basis for selection, as multiple affected generatio ...
... the increased risk of diabetes in their relatives. It is impractical to screen all patients with type 1 diabetes for HNF-1␣ mutations, and a selective approach to identify individuals for HNF-1␣ sequencing is required. We used family history as the basis for selection, as multiple affected generatio ...
Brief Introduction of Bioinformatics
... The technique of dynamic programming can be applied to produce global alignments via the Needleman-Wunsch algorithm, and local alignments via the Smith-Waterman algorithm. In typical usage, protein alignments use a substitution matrix to assign scores to amino-acid matches or mismatches, and a gap p ...
... The technique of dynamic programming can be applied to produce global alignments via the Needleman-Wunsch algorithm, and local alignments via the Smith-Waterman algorithm. In typical usage, protein alignments use a substitution matrix to assign scores to amino-acid matches or mismatches, and a gap p ...
Mutations
... Sickle cell disease is a disorder associated with changes in the shape of red blood cells. Normal red blood cells are round. Sickle cells appear long and pointed. Sickle cell disease is caused by a point mutation in one of the polypeptides found in hemoglobin, the blood’s principal oxygencarrying pr ...
... Sickle cell disease is a disorder associated with changes in the shape of red blood cells. Normal red blood cells are round. Sickle cells appear long and pointed. Sickle cell disease is caused by a point mutation in one of the polypeptides found in hemoglobin, the blood’s principal oxygencarrying pr ...
Brand, Veronica - Degenerate Primer Design using Computational Tools
... Methods. iCODEHOP is an interactive web-based program that incorporates multiple webbased programs and exhibits transparency in the information it uses to design the primers. Thus, the user has access to a large number of inputs and parameters. Although many more options are available, I will only o ...
... Methods. iCODEHOP is an interactive web-based program that incorporates multiple webbased programs and exhibits transparency in the information it uses to design the primers. Thus, the user has access to a large number of inputs and parameters. Although many more options are available, I will only o ...
some inconvenient truths about sex chromosome dosage
... proportion of genes that are compensated. In particular, we discuss the potential role of sexual conflict in the evolution of dosage compensation mechanisms. Before we begin, it is worth defining precisely what is meant by sex chromosome dosage compensation. Sex chromosome dosage compensation is a m ...
... proportion of genes that are compensated. In particular, we discuss the potential role of sexual conflict in the evolution of dosage compensation mechanisms. Before we begin, it is worth defining precisely what is meant by sex chromosome dosage compensation. Sex chromosome dosage compensation is a m ...
Waleed Abdellatief Abd elhleem _paper manuscript
... and blood elements prominently involved. These manifestations occur to a variable extent in the individual patient and their activity can change over time [3]. The association between SLE and inflammation emphasise to the importance of cytokine network genes. Tumour necrosis factor (TNF)-α, an impor ...
... and blood elements prominently involved. These manifestations occur to a variable extent in the individual patient and their activity can change over time [3]. The association between SLE and inflammation emphasise to the importance of cytokine network genes. Tumour necrosis factor (TNF)-α, an impor ...
RNA-Seq
RNA-seq (RNA sequencing), also called whole transcriptome shotgun sequencing (WTSS), is a technology that uses the capabilities of next-generation sequencing to reveal a snapshot of RNA presence and quantity from a genome at a given moment in time.