Development and characterization of DehaloR^2, a novel anaerobic
... gene of Dehalobacter (Schlotelburg et al. 2002) and Desufuromonas (Loffler et al. 2000) primers to verify the presence or absence of these organisms. qPCR targeting the 16S rRNA gene was used to quantify bacteria, Dehalococcoides and Geobacteraceae. Since this region is almost identical across Dehal ...
... gene of Dehalobacter (Schlotelburg et al. 2002) and Desufuromonas (Loffler et al. 2000) primers to verify the presence or absence of these organisms. qPCR targeting the 16S rRNA gene was used to quantify bacteria, Dehalococcoides and Geobacteraceae. Since this region is almost identical across Dehal ...
Evolutionary history of the genus Capra
... methods using MrBayes V 3.0b4 (Huelsenbeck and Ronquist, 2001). For the MP and NJ search, trees were constructed with each locus separately and with both loci together. Since the same trees were obtained in all cases using these methods, data from both loci were analyzed together in the ML and Bayes ...
... methods using MrBayes V 3.0b4 (Huelsenbeck and Ronquist, 2001). For the MP and NJ search, trees were constructed with each locus separately and with both loci together. Since the same trees were obtained in all cases using these methods, data from both loci were analyzed together in the ML and Bayes ...
Sequence and phylogenetic analysis of the gp200 protein of
... Fig. 5. The specificity and detection limit of the gp200-targeted PCR amplification using the primer set EC200-F3/R3. (A) The DNA extracted from blood samples of dogs infected by different rickettsia (Lane 1: E. canis, Lane 2: E. chaffeensis, Lane 3: E. ewingii, Lane 4: A. platys, Lane 5: B. canis v ...
... Fig. 5. The specificity and detection limit of the gp200-targeted PCR amplification using the primer set EC200-F3/R3. (A) The DNA extracted from blood samples of dogs infected by different rickettsia (Lane 1: E. canis, Lane 2: E. chaffeensis, Lane 3: E. ewingii, Lane 4: A. platys, Lane 5: B. canis v ...
Text S1.
... [2] or the 1000 Genomes Project [3]). Putatively functional genotypes are only counted if they occur in genes annotated as being associated with the phenotype. Second layer. These nodes represent genes, split into those annotated as high penetrance GH or low penetrance GL. Their values depend on lin ...
... [2] or the 1000 Genomes Project [3]). Putatively functional genotypes are only counted if they occur in genes annotated as being associated with the phenotype. Second layer. These nodes represent genes, split into those annotated as high penetrance GH or low penetrance GL. Their values depend on lin ...
It`s In Your Genes
... This exercise deals with the replication of DNA and its importance to the process of heredity. Locate the cylindrical coil in the trunk and stretch it out by twisting the two ends in opposite directions. The cylindrical coil is a model of a DNA molecule. The model is many orders of magnitude large ...
... This exercise deals with the replication of DNA and its importance to the process of heredity. Locate the cylindrical coil in the trunk and stretch it out by twisting the two ends in opposite directions. The cylindrical coil is a model of a DNA molecule. The model is many orders of magnitude large ...
SNP
... alleles (0.1% of total SNPs is tri-allelic markers in TSC data). SNPs has lower mutation rate than do repeat sequences, but not as informative as microsatellite markers. detection methods for SNPs are potentially more suitable for genetic screening in automated and large-scale. the SNPs are likely b ...
... alleles (0.1% of total SNPs is tri-allelic markers in TSC data). SNPs has lower mutation rate than do repeat sequences, but not as informative as microsatellite markers. detection methods for SNPs are potentially more suitable for genetic screening in automated and large-scale. the SNPs are likely b ...
Tumor metastasis-associated human MTA1 gene and its MTA1
... in the process of cellular proliferation. Epithelial cancers that over-expressed MTA1 RNA showed significantly higher rates of invasion and lymph node metastasis and tended to have higher rates of vascular involvement. In addition, the MTA1 gene was found to be over-expressed in invasive and metasta ...
... in the process of cellular proliferation. Epithelial cancers that over-expressed MTA1 RNA showed significantly higher rates of invasion and lymph node metastasis and tended to have higher rates of vascular involvement. In addition, the MTA1 gene was found to be over-expressed in invasive and metasta ...
Synonymous codon usage patterns in different parasitic
... many genes has been documented in many species. It has been reported that synonymous codon usage bias may be associated with various factors, such as mutational pressure and compositional constraints (Osawa et al., 1988), translational selection (Sharp and Li, 1986), gene function (Chiapello et al., ...
... many genes has been documented in many species. It has been reported that synonymous codon usage bias may be associated with various factors, such as mutational pressure and compositional constraints (Osawa et al., 1988), translational selection (Sharp and Li, 1986), gene function (Chiapello et al., ...
A siderophore biosynthesis gene cluster from the fish
... DAHP, 3-deoxy-D-arabino-heptulosonate-7-phosphate; HPI, highpathogenicity island; NRPS, non-ribosomal peptide synthetase; SSH, suppression subtractive hybridization. The GenBank/EMBL/DDBJ accession number for the sequences reported in this paper is AJ699306. ...
... DAHP, 3-deoxy-D-arabino-heptulosonate-7-phosphate; HPI, highpathogenicity island; NRPS, non-ribosomal peptide synthetase; SSH, suppression subtractive hybridization. The GenBank/EMBL/DDBJ accession number for the sequences reported in this paper is AJ699306. ...
Inheriting two copies of mutated genes that are
... individuals that are homozygous recessive for the nonfunctional allele. Because the gene is essential, these individuals might fail to develop past fertilization, die in utero, or die later in life, depending on what life stage requires this gene. An inheritance pattern in which an allele is only le ...
... individuals that are homozygous recessive for the nonfunctional allele. Because the gene is essential, these individuals might fail to develop past fertilization, die in utero, or die later in life, depending on what life stage requires this gene. An inheritance pattern in which an allele is only le ...
When the two levels of red and green are
... nucleotide bases: adenine, cytosine, guanine and thymine (abbreviated as A, C, G and T respectively). These bases join into two complementary pairs, with A only binding to T and C with G. The bases are arranged in a double stranded helix (the backbone of the strands being made up of phosphate, the 5 ...
... nucleotide bases: adenine, cytosine, guanine and thymine (abbreviated as A, C, G and T respectively). These bases join into two complementary pairs, with A only binding to T and C with G. The bases are arranged in a double stranded helix (the backbone of the strands being made up of phosphate, the 5 ...
BLOTTING.142
... Size-separation of the fragments (standard agarose gel electrophoresis) In situ denaturation of the DNA fragments (by incubation @ ↑temp) Transfer of denatured DNA fragments into a solid support (nylon or ...
... Size-separation of the fragments (standard agarose gel electrophoresis) In situ denaturation of the DNA fragments (by incubation @ ↑temp) Transfer of denatured DNA fragments into a solid support (nylon or ...
Slides
... If you fnd a paper describing a genome-wide association study on your disease of interest, please look up the paper and report to me 1) the URL or UID of the paper and 2) genes or SNPs that are most highly correlated with the disease. 3) the odds ratio and heritability of each gene and 4) Also pleas ...
... If you fnd a paper describing a genome-wide association study on your disease of interest, please look up the paper and report to me 1) the URL or UID of the paper and 2) genes or SNPs that are most highly correlated with the disease. 3) the odds ratio and heritability of each gene and 4) Also pleas ...
ovo D1
... Mutations: the lifeblood of genetic analysis a Morgan "student": (1) What kinds can we make? (functional categories) Herman Muller (1930s): inferred how mutations can affect gene functioning. ...
... Mutations: the lifeblood of genetic analysis a Morgan "student": (1) What kinds can we make? (functional categories) Herman Muller (1930s): inferred how mutations can affect gene functioning. ...
Received June 19, 1964.
... indicate that uridylic acid should be labelled prior to cytidylic acid upon addition of any previous precursor. Table I shows that in all cases where their labelling was measurable, the specific activity of uridylic acid was higher than that of cytidylic acid. In other unpublished experiments with c ...
... indicate that uridylic acid should be labelled prior to cytidylic acid upon addition of any previous precursor. Table I shows that in all cases where their labelling was measurable, the specific activity of uridylic acid was higher than that of cytidylic acid. In other unpublished experiments with c ...
Cloning and expression of the phosphotriesterase
... Increased expression of hocA was observed from an integrative hocA–lacZ fusion when cultures were grown in the absence of phosphate, suggesting that it might be part of the Pho regulon, but the phosphate-regulated promoter was not cloned in this study. This is believed to be the first study in which ...
... Increased expression of hocA was observed from an integrative hocA–lacZ fusion when cultures were grown in the absence of phosphate, suggesting that it might be part of the Pho regulon, but the phosphate-regulated promoter was not cloned in this study. This is believed to be the first study in which ...
Not all mutant phenotypes are equally informative. Forward genetics
... fact: it’s easier to mess things up than to make them better ...
... fact: it’s easier to mess things up than to make them better ...
The universal reagent for genome tailoring
... control that the remaining loxP site does not create a disturbance in the gene function after the selectable marker is removed. Interestingly, this allele without the marker removal (Fig. 3B) can provide a useful tool for learning more about the function of the gene of interest. If the marker was de ...
... control that the remaining loxP site does not create a disturbance in the gene function after the selectable marker is removed. Interestingly, this allele without the marker removal (Fig. 3B) can provide a useful tool for learning more about the function of the gene of interest. If the marker was de ...
Anterior-posterior patterning within the
... ges-1∆B expression is observed from the 16E-cell stage onward; expression is always intense in int1, is often detected in int2 but at lower levels and can occasionally be detected in int3 and int4, but only after prolonged staining. For the current paper, embryos were examined at the 16- to 20-E-cel ...
... ges-1∆B expression is observed from the 16E-cell stage onward; expression is always intense in int1, is often detected in int2 but at lower levels and can occasionally be detected in int3 and int4, but only after prolonged staining. For the current paper, embryos were examined at the 16- to 20-E-cel ...
Haplotypes at LBX1 Have Distinct Inheritance Patterns with
... (P<5×10–8). Results for the 30 most strongly AIS-associated SNPs from the GWAS are shown in Table A in S2 File. Through imputation of the LBX1 region we identified 10 additional markers that passed the threshold of Ptrend = 5×10–7 as shown in Table 1 (excluding markers in perfect LD). The table incl ...
... (P<5×10–8). Results for the 30 most strongly AIS-associated SNPs from the GWAS are shown in Table A in S2 File. Through imputation of the LBX1 region we identified 10 additional markers that passed the threshold of Ptrend = 5×10–7 as shown in Table 1 (excluding markers in perfect LD). The table incl ...
Full Text
... blot analysis revealed that the number of double heterozygous weanlings was significantly reduced below the number expected based on the additive effects predicted from the observed losses due to heterozygosity at the individual loci (Table 1). Incompletely penetrant heterozygous defects have been r ...
... blot analysis revealed that the number of double heterozygous weanlings was significantly reduced below the number expected based on the additive effects predicted from the observed losses due to heterozygosity at the individual loci (Table 1). Incompletely penetrant heterozygous defects have been r ...
Mendel and Punnet Square Quiz - etec-510-2011
... d) Genes are inherited from parents, but the question asks for the definition of genotype. 14. Phenotype refers to a) what genes the individual possess b) the physical appearance of the individual c) dominant alleles of the individual d) homozygous genes from the parents Formative feedback a) The g ...
... d) Genes are inherited from parents, but the question asks for the definition of genotype. 14. Phenotype refers to a) what genes the individual possess b) the physical appearance of the individual c) dominant alleles of the individual d) homozygous genes from the parents Formative feedback a) The g ...
Boundary elements and nuclear organization
... 3. Insulators and transcriptional regulation The ability of boundary elements to prevent repression by blocking the spread of heterochromatin has been described for a variety of identified insulator elements (for a list of such elements, see West et al., 2002), suggestive of their role in preserving ...
... 3. Insulators and transcriptional regulation The ability of boundary elements to prevent repression by blocking the spread of heterochromatin has been described for a variety of identified insulator elements (for a list of such elements, see West et al., 2002), suggestive of their role in preserving ...
(type I) and mannose-resistant F8 (P) fimbriae of Escherichia coli
... shown in this paper the genetic determinants of F8 fimbriae were m a p p e d at a position between 17--20 on the chromosome of strain 2980 of E. coli O18:K5. Therefore, it seems that the gene clusters coding for type I fimbriae are located at fixed positions on the chromosomes of different strains. ...
... shown in this paper the genetic determinants of F8 fimbriae were m a p p e d at a position between 17--20 on the chromosome of strain 2980 of E. coli O18:K5. Therefore, it seems that the gene clusters coding for type I fimbriae are located at fixed positions on the chromosomes of different strains. ...
Intervention strategies for mitochondrial disease
... mitochondrial disorder in a single patient with hypermetabolism, has now evolved into the discipline of mitochondrial medicine • Lessons learned from studies of rare diseases have implications for a broad range of medical disciplines • Within the next few years, the application of new technologies ( ...
... mitochondrial disorder in a single patient with hypermetabolism, has now evolved into the discipline of mitochondrial medicine • Lessons learned from studies of rare diseases have implications for a broad range of medical disciplines • Within the next few years, the application of new technologies ( ...
RNA-Seq
RNA-seq (RNA sequencing), also called whole transcriptome shotgun sequencing (WTSS), is a technology that uses the capabilities of next-generation sequencing to reveal a snapshot of RNA presence and quantity from a genome at a given moment in time.