Expanded roles of leucine-responsive regulatory protein in
... fluid), and then resuspended in IF-0 for PM plates 1 and 2, in IF-0 containing 20 mM sodium succinate and 2 mM ferric citrate for PM plates 3–8, and in IF-10 containing 2.0 g tryptone, 1.0 g yeast extract and 1.0 g NaCl l–1 at a density corresponding to 85% transmittance (OD420*0.12) using a 20 mm d ...
... fluid), and then resuspended in IF-0 for PM plates 1 and 2, in IF-0 containing 20 mM sodium succinate and 2 mM ferric citrate for PM plates 3–8, and in IF-10 containing 2.0 g tryptone, 1.0 g yeast extract and 1.0 g NaCl l–1 at a density corresponding to 85% transmittance (OD420*0.12) using a 20 mm d ...
Massive Changes in Genome Architecture Accompany
... The suppression of recombination across such a region will be selected for if it creates linkage between the sex-determining locus and other genes that are sexually antagonistic in that their functions are beneficial to only one of the sexes. The nonrecombining region can be formed from the spread of ...
... The suppression of recombination across such a region will be selected for if it creates linkage between the sex-determining locus and other genes that are sexually antagonistic in that their functions are beneficial to only one of the sexes. The nonrecombining region can be formed from the spread of ...
SPATULA, a bHLH carpel development gene
... cDNAs corresponding to florally expressed genes located within the candidate region were isolated by using λMH1, λMH3 and cosMH1 as probes to screen a cDNA library made from Ler inflorescences containing buds up to stage 12 (Weigel et al., 1992). Sequencing of mutant alleles The genomic sequence of ...
... cDNAs corresponding to florally expressed genes located within the candidate region were isolated by using λMH1, λMH3 and cosMH1 as probes to screen a cDNA library made from Ler inflorescences containing buds up to stage 12 (Weigel et al., 1992). Sequencing of mutant alleles The genomic sequence of ...
Mutualism and asexual reproduction influence recognition genes in a fungal... M.A. van der Nest1, E.T. Steenkamp2, P.M.... Wingfield1 and B. Slippers1*
... (dS) substitution rate ratio (Yang and Nielson, 1998). To test for variation of selective pressures across the codons, goodness of fit was calculated for the different site-specific models proposed by Yang et al. (2000). Statistical significance was calculated with likelihood ratio tests (LRT), whic ...
... (dS) substitution rate ratio (Yang and Nielson, 1998). To test for variation of selective pressures across the codons, goodness of fit was calculated for the different site-specific models proposed by Yang et al. (2000). Statistical significance was calculated with likelihood ratio tests (LRT), whic ...
Perspectives in Diabetes Glucokinase Gene Structure
... Comparison of glucok~nasecDNAs isolated from hepatic, the fourth exon of the gene. Deletion of 51 nucleotides from islet, and insulinoma cDNA libraries indicates that different the mRlVA results in an isoform that is missing 17 amino glucokinase isoforms are generated through alternate splic- acids ...
... Comparison of glucok~nasecDNAs isolated from hepatic, the fourth exon of the gene. Deletion of 51 nucleotides from islet, and insulinoma cDNA libraries indicates that different the mRlVA results in an isoform that is missing 17 amino glucokinase isoforms are generated through alternate splic- acids ...
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... (iii) A wealth of functional studies organized into an efficient database. When the complete genome was published [48], we had some functional knowledge of about 30% of the roughly 5800 genes; the figure now reaches practically 85%. All information obtained is centralized in the Saccharomyces Genome ...
... (iii) A wealth of functional studies organized into an efficient database. When the complete genome was published [48], we had some functional knowledge of about 30% of the roughly 5800 genes; the figure now reaches practically 85%. All information obtained is centralized in the Saccharomyces Genome ...
Engineering Acetyl Coenzyme A Supply: Functional Expression of a
... In vivo activity of E. faecalis PDH required simultaneous expression of E. faecalis genes encoding its E1␣, E1, E2, and E3 subunits, as well as genes involved in lipoylation of E2, and addition of lipoate to growth media. A strain lacking ACS that expressed these E. faecalis genes grew at near-wild ...
... In vivo activity of E. faecalis PDH required simultaneous expression of E. faecalis genes encoding its E1␣, E1, E2, and E3 subunits, as well as genes involved in lipoylation of E2, and addition of lipoate to growth media. A strain lacking ACS that expressed these E. faecalis genes grew at near-wild ...
Preferential X-chromosome inactivation, DNA
... embryonic gene coding for the methylase. In the egg the low level of methylation observed suggests that methylase activity might be low. The opposite turns out to be true. Using a highly sensitive microassay for the methylase developed by Roger Adams (Department of Biochemistry, University of Glasgo ...
... embryonic gene coding for the methylase. In the egg the low level of methylation observed suggests that methylase activity might be low. The opposite turns out to be true. Using a highly sensitive microassay for the methylase developed by Roger Adams (Department of Biochemistry, University of Glasgo ...
Observations on the role of nuclear medicine in molecular imaging
... use of large concentrations of tracers; concentrations often large enough to provide toxicity and/or to interfere with the function being measured (for example, by saturating low density sites). In addition, the available numbers of such tracers are limited at present. Both CT and MRI do not involve ...
... use of large concentrations of tracers; concentrations often large enough to provide toxicity and/or to interfere with the function being measured (for example, by saturating low density sites). In addition, the available numbers of such tracers are limited at present. Both CT and MRI do not involve ...
Par-1
... machinery Many components of RNAi machinery have been identified through genetic screening for RNAi defective mutants and through biochemical studies using cell extracts (e.g. Drosophila embryo extract). ...
... machinery Many components of RNAi machinery have been identified through genetic screening for RNAi defective mutants and through biochemical studies using cell extracts (e.g. Drosophila embryo extract). ...
Origins and Early Evolution of the tRNA Molecule
... properties inherent to A, U, G, or C effect a discrimination substitutions are by far more frequent than any cross-type substitutions based on the experiments of complementary from non-complementary nucleotides with a quality factor not exceeding a value with RNA-replicases. Therefore, he assumed on ...
... properties inherent to A, U, G, or C effect a discrimination substitutions are by far more frequent than any cross-type substitutions based on the experiments of complementary from non-complementary nucleotides with a quality factor not exceeding a value with RNA-replicases. Therefore, he assumed on ...
Pultz, M. A., and Baker, B. S.
... is passed from the level of splicing back to the level of transcription. For example, the dsx proteins are zinc finger related transcription factors that bind to sex-specific enhancer sequences in the yolk protein gene (Burtis et al., 1991; Coschigano and Wensink, 1993; Erdman and Burtis, 1993). Mos ...
... is passed from the level of splicing back to the level of transcription. For example, the dsx proteins are zinc finger related transcription factors that bind to sex-specific enhancer sequences in the yolk protein gene (Burtis et al., 1991; Coschigano and Wensink, 1993; Erdman and Burtis, 1993). Mos ...
A role for interleukin-1 in epidermal differentiation
... IL-1 RI or IL-1 RII, the number of these receptors on keratinocytes in vitro was found to vary depending on culture conditions. Total receptor number decreased initially as keratinocyte cultures became confluent and more differentiated (Kupper et al., 1988), although induction of terminal differenti ...
... IL-1 RI or IL-1 RII, the number of these receptors on keratinocytes in vitro was found to vary depending on culture conditions. Total receptor number decreased initially as keratinocyte cultures became confluent and more differentiated (Kupper et al., 1988), although induction of terminal differenti ...
Fitness landscapes of gene regulation in variable - UvA-DARE
... with the optimal Pgal concentrations as obtained directly from the growth data in figure 6.1. Although the optimal level shows a very sharp Pgal dependence, this does not mean that the growth difference for optimal and non-optimal expression are necessarily large. We can see from the landscape in fig. ...
... with the optimal Pgal concentrations as obtained directly from the growth data in figure 6.1. Although the optimal level shows a very sharp Pgal dependence, this does not mean that the growth difference for optimal and non-optimal expression are necessarily large. We can see from the landscape in fig. ...
Molecular characterization of glutathione peroxidase
... GPx2 promoter regions are capable of responding to exogenous agents such as paraquat and t-butyl hydroperoxide (24, 25). This transcriptional response of oxidative stress is likely to be more physiologically relevant in vivo than the post-transcriptional regulation mechanism which occurs in vitro in ...
... GPx2 promoter regions are capable of responding to exogenous agents such as paraquat and t-butyl hydroperoxide (24, 25). This transcriptional response of oxidative stress is likely to be more physiologically relevant in vivo than the post-transcriptional regulation mechanism which occurs in vitro in ...
A genetically encoded fluorescent tRNA is active in live
... complexity of tRNA biology, it is necessary to have the tools to monitor tRNA location and activity inside living cells, both spatially and temporally. However, current methodologies of live-cell imaging tRNA have limitations. Typically, a specific tRNA is isolated from the yeast bulk, labeled with ...
... complexity of tRNA biology, it is necessary to have the tools to monitor tRNA location and activity inside living cells, both spatially and temporally. However, current methodologies of live-cell imaging tRNA have limitations. Typically, a specific tRNA is isolated from the yeast bulk, labeled with ...
RESISTANCE AS A RESPONSE TO STRESS
... altered to yield resistance, then susceptible and resistant strains will have quantitatively different amounts of that P450. Three P450s have been demonstrated to be over-expressed by resistant insect strains: P450Lpr (Wheelock & Scott 1992), CYP6A1 (Cariño et al. 1994), and CYP6A2 (Waters et al. 19 ...
... altered to yield resistance, then susceptible and resistant strains will have quantitatively different amounts of that P450. Three P450s have been demonstrated to be over-expressed by resistant insect strains: P450Lpr (Wheelock & Scott 1992), CYP6A1 (Cariño et al. 1994), and CYP6A2 (Waters et al. 19 ...
microbial genetics
... plasmid monomers (Fig.4b). This ensures that all copies of the plasmid partition independently. While Cer increases the stability of ColE1 plasmids, it is not essential for maintenance of ColE1 in a population. For the low copy number plasmids, partition of plasmid molecules into daughter cells is m ...
... plasmid monomers (Fig.4b). This ensures that all copies of the plasmid partition independently. While Cer increases the stability of ColE1 plasmids, it is not essential for maintenance of ColE1 in a population. For the low copy number plasmids, partition of plasmid molecules into daughter cells is m ...
Recurrent Pregnancy Loss and Its Relation to Combined Parental
... Current results showed the maternal mutated thrombophilic genes’ profiles mainly have a potential risk for RPL but paternal mutation profiles in specific genes may also have a combined effect on RPL phenomenon. Study of mutations in a wide range of thrombophilic genes associated with the disease m ...
... Current results showed the maternal mutated thrombophilic genes’ profiles mainly have a potential risk for RPL but paternal mutation profiles in specific genes may also have a combined effect on RPL phenomenon. Study of mutations in a wide range of thrombophilic genes associated with the disease m ...
Epistasis Quantitative Trait Loci and Evidence for 4 Region into
... the C4R3 genotype influences the anti-MOG Ab levels of the IgG1 isotype, demonstrating a qualitative effect on the autoimmune response (8, 21). A high-resolution mapping of MOG-EAE was performed in an advanced intercross line (AIL), which allows the separation of closely situated QTLs as well as det ...
... the C4R3 genotype influences the anti-MOG Ab levels of the IgG1 isotype, demonstrating a qualitative effect on the autoimmune response (8, 21). A high-resolution mapping of MOG-EAE was performed in an advanced intercross line (AIL), which allows the separation of closely situated QTLs as well as det ...
Genetic Analysis of Salt Tolerance in Arabidopsis
... and Zhu, 1997). Several salt-inducible genes show increased expression in sos1 mutants under salt stress (Liu and Zhu, 1997a). We have since completed a large-scale screening (z260,000 plants) in an attempt to identify all genetic loci mutations in which can result an sos phenotype. Altogether, 42 s ...
... and Zhu, 1997). Several salt-inducible genes show increased expression in sos1 mutants under salt stress (Liu and Zhu, 1997a). We have since completed a large-scale screening (z260,000 plants) in an attempt to identify all genetic loci mutations in which can result an sos phenotype. Altogether, 42 s ...
multicopy gene family evolution on primate y chromosomes
... In the broadest sense, my thesis investigates two related questions: How are Y chromosomes different from autosomes?; and, Why are two Y chromosomes from different species often so dissimilar from one another, even when their autosomes are very similar? These questions have plagued geneticists and e ...
... In the broadest sense, my thesis investigates two related questions: How are Y chromosomes different from autosomes?; and, Why are two Y chromosomes from different species often so dissimilar from one another, even when their autosomes are very similar? These questions have plagued geneticists and e ...
robbie0306
... Method due to George and Robbie Take two networks and scramble the name mapping between them. ...
... Method due to George and Robbie Take two networks and scramble the name mapping between them. ...
Ethylene is involved in strawberry fruit ripening in an organ
... 1997; Aharoni and O’Connell, 2002; Bombarely et al., 2010; Csukasi et al., 2011, 2012), little is known about the regulation of these processes, including the role played by the various phytohormones. The main reason for this relatively poor understanding in comparison with other fruits is that what ...
... 1997; Aharoni and O’Connell, 2002; Bombarely et al., 2010; Csukasi et al., 2011, 2012), little is known about the regulation of these processes, including the role played by the various phytohormones. The main reason for this relatively poor understanding in comparison with other fruits is that what ...
RNA-Seq
RNA-seq (RNA sequencing), also called whole transcriptome shotgun sequencing (WTSS), is a technology that uses the capabilities of next-generation sequencing to reveal a snapshot of RNA presence and quantity from a genome at a given moment in time.