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Genomic structure, chromosomal localization, and conserved alternative splice forms of thrombopoietin
Genomic structure, chromosomal localization, and conserved alternative splice forms of thrombopoietin

Identification of Novel Non-Metal Haloperoxidases from the Marine
Identification of Novel Non-Metal Haloperoxidases from the Marine

... However, to our knowledge, there have been very few reported studies that demonstrates enzyme activity with ...
Aberrant DNA methylation in cancer: potential clinical
Aberrant DNA methylation in cancer: potential clinical

... and not genetic, changes, as although they affect the structure of DNA they do not materially affect the genetic code. The human genome exhibits a clear depletion of CpG dinucleotides, presumably due to the high rate of deamination of 5-methylcytosine to thymine (Ref. 1). However, the genome also co ...
alpha-amylase from bacillus licheniformis
alpha-amylase from bacillus licheniformis

... The LE399 α-amylase is an enzyme with improved specificity and specific activity as well as stability at low pH and low calcium concentration at high temperatures in comparison with other α-amylases. Alpha-amylases used in food processing require calcium ions for stability. In industrial application ...
Creation/Evolution - Geoscience Research Institute
Creation/Evolution - Geoscience Research Institute

... randomness in assignment of amino acids to codons No mechanism exists for genetic code evolution Thus variation in the genetic code suggests a polyphyletic origin for life Taken together, this evidence indicates the hand of a Designer in the genetic code and does not support the theory that life ori ...
A genome-wide association scan in pig identifies novel regions
A genome-wide association scan in pig identifies novel regions

... It was assumed that most markers had very small effects on the trait (99.5% of SNP in this analysis) and only a few markers (0.5%) had large effects. Therefore, among the total 30,847 SNP, a priori about 154 SNP were assumed to have an effect on FCR. The allocation of each SNP to either of these 2 d ...
PDF
PDF

... In a diverse range of species, local rates of crossing-over correlate with genetic diversity but not with genetic divergence [28,29]. These correlations are inferred to be due to an indirect effect of recombination due to the interaction between selection and linkage and their strength can be used t ...
Concordance of Changes in Metabolic Pathways Based
Concordance of Changes in Metabolic Pathways Based

... ultra-performance liquid chromatography quadruple timeof-flight mass spectrometry (UPLC-ToF MS)–based comprehensive metabolomic profiling approach was found to discriminate between impaired and normal glucose tolerance (15). These emerging technologies have enabled researchers to identify biomarkers ( ...
The Art of Multiple Sequence Alignment in R - decipher
The Art of Multiple Sequence Alignment in R - decipher

Molecular Characterisation of the 76 kDa Iron
Molecular Characterisation of the 76 kDa Iron

... parts of the 76 kDa subunit homologues. Whereas the mature peptide sequences from most other species align to a common N-terminus, the potato 76 kDa subunit has an ...
Rapid generation of nested chromosomal
Rapid generation of nested chromosomal

... Fig. 2. Strategy for generation of nested deletions on mouse chromosome 2 by a combined targeted兾random approach. (A) The Notch1 gene was targeted to insert a cassette consisting of neo, a loxP site, and tk. A targeted line was selected, and a linearized vector consisting of a stuffer fragment of b ...
Genetic testing for asthma REVIEW
Genetic testing for asthma REVIEW

... CYF1P2 [7] and HLA-G [8], are currently subject to replication studies. If these genes are confirmed in other populations, as recently shown for the gene encoding G-protein coupled receptor A (Neuropeptide S receptor-1) [7, 9–11], new insights into the pathogenesis of asthma will be obtained in the ...
Single Nucleotide Polymorphism Detection with the iPLEX® Assay
Single Nucleotide Polymorphism Detection with the iPLEX® Assay

... enabling flexible assay design and fast time-to-results (Figure 1). Capable of easily interrogating from 10 to as many as 400 single nucleotide polymorphisms (SNPs) per sample, and analyzing from just a few samples to hundreds of samples per day, the MassARRAY System can be used on its own or in con ...
Molecular Biology Reports
Molecular Biology Reports

... extracted with the Easy-Spin total RNA extraction kit (iNtRON, Korea), according to the manufacturer’s instructions. For quantitative expression analysis of genes involved in carotenoid biosynthesis, first-strand cDNA was generated from total RNA (2 lg) using MMLV reverse transcriptase (Promega, USA ...
Pourcel et al., Microbiology 2005
Pourcel et al., Microbiology 2005

... CRISPR polymorphism in Y. pseudotuberculosis Y. pseudotuberculosis represents an older, more diverged species from which Y. pestis probably emerged as a clone (Achtman et al., 1999). The three CRISPRs are present at least in some members of this species, and can be amplified using the Y. pestis prim ...
Table of Contents - Scholars` Bank
Table of Contents - Scholars` Bank

... isoaccepting tRNA group, one codon may be read by two individual tRNAs each interacting in one of the two ways. This leads to the question: Why would such redundancy occur? Through the inactivation of the gene that encodes for the threonine isoaccepting tRNA with the anticodon CGU (tRNA-CGU), it was ...
Untitled - System Components
Untitled - System Components

... Circadian clock: the circadian clock is an endogenous, internally maintained biochemical oscillation with a period of approximately a day. Circadian rhythms consist of two elements, a period and an amplitude of oscillation, without which there is no rhythm. The circadian clock is involved in the dai ...
A Genetic Linkage Map of Mouse Chromosome 10
A Genetic Linkage Map of Mouse Chromosome 10

... -1.7 f 1.0-Esr-4.6 f 2.0-[Myb,Ahi-11-5.6 f 2.2Braf-0.9 k 0.9-Fyn-5.6 f 2.2-Ros-1-3.7 f l.8-Cos1-4.6 f 2.0-Cdc-2a-0.9 f 0.9-Bcr-0.9 f 0.9-[Co16a1, Co16a-2, (SIOOb)]-7.4 k 2.5-Pah-0.9 f 0.9-Id-124.1 f 4.1-Ifg-0.9 f 0.9-pgCh"-0.9 f 0.9-Gli. The Bcr locus was mapped initially using a mouse cDNA probecor ...
this PDF file - E-Journal Faculty of Medicine Universitas
this PDF file - E-Journal Faculty of Medicine Universitas

PCB Biodegradation and bphA1 Gene Expression Induced by
PCB Biodegradation and bphA1 Gene Expression Induced by

... them, BphA1 plays a crucial role in determination of substrate specificity and biodegradation efficiency for PCB degraders [7]. Particularly, the bphA1 gene encoding the large subunit of iron-sulfur protein of BphA with a substrate-binding site may be used as a molecular mark to evaluate substrate s ...
Association of functionally significant Melanocortin
Association of functionally significant Melanocortin

... an EC50 that was significantly different (P , 0.05) compared with that of the WT receptor, and/or (3) a mutation had an Emax ,50% of WT receptor maximum activity. Including the 2 nonsense and 2 deletion mutations, 10 mutations, found only in obese subjects (in a total of 12 obese carriers), modified ...
Loss of Hox-A1 (Hox-1.6) function results in the
Loss of Hox-A1 (Hox-1.6) function results in the

... embryos revealed highly organized patterns of sensory fiber and motor neuron labeling. Dye injections were made between E11.5 and E12.5 shortly after the disappearance of morphologically identifiable rhombomeres. However, based on observations in chick (Lumsden and Keynes, 1989) and mouse (Marshall ...
emboj7601705-sup
emboj7601705-sup

... stage 13. For RNA extraction and cDNA synthesis, 8 DMZs were used per sample. To study the responses in gene expression upon increasing doses of Gsc MO, quantitative RT-PCRs for marker genes of prechordal plate (Frzb1) and anterior brain (Otx2), notochord (Collagen type IIA) and somites (MyoD), as w ...
Genetic analysis of the floral initiation process (FLIP) in Arabidopsis
Genetic analysis of the floral initiation process (FLIP) in Arabidopsis

... 3° depending on their origin, as described in the text. = rosette leaf; = inflorescence leaf; = inflorescence shoot (1° or 2°); = flower. ...
PDF
PDF

... short-lived transcription repressors that involve in the regulation of auxin signaling (Guilfoyle and Hagen, 2007; Guilfoyle, 2015). Most of the Aux/IAA proteins contain four conserved domains, namely, Domain I, Domain II, Domain III, and Domain IV. Domain I is an active repression domain containing ...
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RNA-Seq



RNA-seq (RNA sequencing), also called whole transcriptome shotgun sequencing (WTSS), is a technology that uses the capabilities of next-generation sequencing to reveal a snapshot of RNA presence and quantity from a genome at a given moment in time.
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