
A mutation in the Arabidopsis mTERFrelated plastid protein
... Transcript levels were determined relative to the wild type in seedlings first grown for 5 days under continuous light, then transferred to the dark for 8 h and re-exposed to light for 30 min. The relative mRNA abundance was normalized to the corresponding ACTI2 (At3g18780) transcript level. The val ...
... Transcript levels were determined relative to the wild type in seedlings first grown for 5 days under continuous light, then transferred to the dark for 8 h and re-exposed to light for 30 min. The relative mRNA abundance was normalized to the corresponding ACTI2 (At3g18780) transcript level. The val ...
Molecular Diagnostics for the Detection and Characterization of
... The technical feasibility of these assays has already been demonstrated. How these new, often more expensive tests will be incorporated into routine practice and the impact they will have on patient care remain to be determined. One of the most attractive uses for such techniques is to achieve a mor ...
... The technical feasibility of these assays has already been demonstrated. How these new, often more expensive tests will be incorporated into routine practice and the impact they will have on patient care remain to be determined. One of the most attractive uses for such techniques is to achieve a mor ...
x-linked female-sterile loci in drosophzla melanogaster
... per locus from the two independent screens are similar (Figure 1). Using the data from these mutagenesis screens, the number of loci on the X chromosome has been estimated to be about 100 (KING and MOHLER 1975) (however, see DISCUSSION for a modification of this estimate). If this estimate reflects ...
... per locus from the two independent screens are similar (Figure 1). Using the data from these mutagenesis screens, the number of loci on the X chromosome has been estimated to be about 100 (KING and MOHLER 1975) (however, see DISCUSSION for a modification of this estimate). If this estimate reflects ...
Model-Based Clustering for Expression Data via a Dirichlet Process
... heuristic schemes, such as the ability to assess uncertainty about the resulting clustering and to formally estimate the number of clusters. This chapter describes a model-based clustering procedure for microarray expression data based on a well-defined statistical model, specifically, a conjugate D ...
... heuristic schemes, such as the ability to assess uncertainty about the resulting clustering and to formally estimate the number of clusters. This chapter describes a model-based clustering procedure for microarray expression data based on a well-defined statistical model, specifically, a conjugate D ...
Genetic characterization of diplodia resistant in somatic hybrids Citrus unshiu (RGA)
... Citrus is one important crop in agroindustrial. Good quality is one important thing that required citrus to be competitive crop in agroindustrial. Research Institute of Citrus and Subtropic Fruit had efforts to increase quality of citrus in Indonesia. Protoplast fusion of Citrus unshiu and Citrus no ...
... Citrus is one important crop in agroindustrial. Good quality is one important thing that required citrus to be competitive crop in agroindustrial. Research Institute of Citrus and Subtropic Fruit had efforts to increase quality of citrus in Indonesia. Protoplast fusion of Citrus unshiu and Citrus no ...
Potential virulence and antimicrobial susceptibility of
... Aeromonas were also present in the strains of A. popo¤i tested (Table 3). All strains had the genes for DNases, GCAT and lipases and showed DNase and lipase activity. Although the role of DNases is unknown in Aeromonas pathogenicity, these genes are involved in Streptococcus infections [25], and are ...
... Aeromonas were also present in the strains of A. popo¤i tested (Table 3). All strains had the genes for DNases, GCAT and lipases and showed DNase and lipase activity. Although the role of DNases is unknown in Aeromonas pathogenicity, these genes are involved in Streptococcus infections [25], and are ...
Feline Genetics: a Combinatorial Approach
... the cell and the other to the opposite end: which goes where is random. When this process is completed, at one end there are 19 chromosome and at the other there are their homologous chromosomes, but the selection is random. Now, as in the mitosis, the cell splits in two cells, each of which possess ...
... the cell and the other to the opposite end: which goes where is random. When this process is completed, at one end there are 19 chromosome and at the other there are their homologous chromosomes, but the selection is random. Now, as in the mitosis, the cell splits in two cells, each of which possess ...
The amelogenin gene
... Lau et al. (1989) mapped human amelogenin genes on the short arm of the X-chromosome in the p22.1-p22.3 region and in the peri centric region of the V-chromosome. The amelogenin sequence on the human V-chromosome was tentatively mapped onto the proximal long arm in the Yq11 region, on the basis of r ...
... Lau et al. (1989) mapped human amelogenin genes on the short arm of the X-chromosome in the p22.1-p22.3 region and in the peri centric region of the V-chromosome. The amelogenin sequence on the human V-chromosome was tentatively mapped onto the proximal long arm in the Yq11 region, on the basis of r ...
Cytochrome P450 gene test to establish the correct warfarin dose in
... polymorphisms which affect warfarin metabolism and dosing. There are many studies which identify the types of variants and their frequencies, however there is a lack of data describing the clinical utility of genotype-guided dosing. That is, by identifying a patient’s genotype, is the amount of time ...
... polymorphisms which affect warfarin metabolism and dosing. There are many studies which identify the types of variants and their frequencies, however there is a lack of data describing the clinical utility of genotype-guided dosing. That is, by identifying a patient’s genotype, is the amount of time ...
Ribosomal proteins promote leaf adaxial identity
... required for the normal leaf polarity (Huang et al., 2006). Here, we implicate a new class of genes, the ribosomal large subunit protein encoding genes, in leaf polarity establishment. In animals, functions of the ribosomal proteins have been investigated extensively, and defects in these proteins c ...
... required for the normal leaf polarity (Huang et al., 2006). Here, we implicate a new class of genes, the ribosomal large subunit protein encoding genes, in leaf polarity establishment. In animals, functions of the ribosomal proteins have been investigated extensively, and defects in these proteins c ...
Cloning of a T-Type Ca Channel Isoform in I n s u l i n
... -cells was examined in rat brain, heart, kidney, and liver by using an RT-PCR assay. The primers used for the RT-PCR were 5 -GAAGATGCGAGTGGACAG-3 (forward) and 5 -CTGTGGCGATGGTCACTG-3 (backward). The PCR products were detected by agarose gel electrophoresis on a 1% gel. Genome walking. The genome wa ...
... -cells was examined in rat brain, heart, kidney, and liver by using an RT-PCR assay. The primers used for the RT-PCR were 5 -GAAGATGCGAGTGGACAG-3 (forward) and 5 -CTGTGGCGATGGTCACTG-3 (backward). The PCR products were detected by agarose gel electrophoresis on a 1% gel. Genome walking. The genome wa ...
Complete comparative genomic analysis of two field isolates of
... (v90/4, 15?0–27?1 kb; v90/2, 15?0–28?3 kb). In this 12?1 kb region (7?7 % of the genome), there are 261 of the 521 point mutations, accounting for 50 % of the total nucleotide changes. Of the 261 point mutations, 82 cause nonsynonymous changes and only the chitinase and orf27 genes do not have any a ...
... (v90/4, 15?0–27?1 kb; v90/2, 15?0–28?3 kb). In this 12?1 kb region (7?7 % of the genome), there are 261 of the 521 point mutations, accounting for 50 % of the total nucleotide changes. Of the 261 point mutations, 82 cause nonsynonymous changes and only the chitinase and orf27 genes do not have any a ...
Altered Pax gene expression in murine notochord mutants: the
... is restricted to the dorsal aspect of both somite and neural tube (Goulding et al., 1991, 1993). The mouse Pax-3 mutation Splotch demonstrates the importance of this gene for the development of dorsal structures, as the dorsally emerging neural crest cells are defective and the neural tube frequentl ...
... is restricted to the dorsal aspect of both somite and neural tube (Goulding et al., 1991, 1993). The mouse Pax-3 mutation Splotch demonstrates the importance of this gene for the development of dorsal structures, as the dorsally emerging neural crest cells are defective and the neural tube frequentl ...
Eds., N. Hamamura, S. Suzuki, S. Mendo, C. M. Barroso,... © by TERRAPUB, 2010.
... in the culture media increased. No apparent differences were detected on the expression levels of sugE clone (sugE clone), suggesting that in this clone this gene is constitutively expressed. As expected, in clone pUC19 (plasmid vector with no insert), no amplification was observed. Two different ne ...
... in the culture media increased. No apparent differences were detected on the expression levels of sugE clone (sugE clone), suggesting that in this clone this gene is constitutively expressed. As expected, in clone pUC19 (plasmid vector with no insert), no amplification was observed. Two different ne ...
ST.25 - WIPO
... (iii) if furnished on paper in accordance with Rule 13ter.1(b), shall have independent page numbering; (iv) if furnished in electronic form, shall be in an electronic document format and filed by a means of transmittal in accordance with paragraph 39; (v) if furnished in electronic form together wit ...
... (iii) if furnished on paper in accordance with Rule 13ter.1(b), shall have independent page numbering; (iv) if furnished in electronic form, shall be in an electronic document format and filed by a means of transmittal in accordance with paragraph 39; (v) if furnished in electronic form together wit ...
Ret/PTC3 is the most frequent form of gene rearrangement
... papillary thyroid carcinomas only for ret/PTC1 rearrangements and found no rearrangement; Ishizaka et al. (1991) found one ret/PTC1 rearrangement among 11 carcinomas, and Wajjwalku et al. (1992) found only one such alteration among 38 carcinomas. On the basis of those observations, RET gene rearrang ...
... papillary thyroid carcinomas only for ret/PTC1 rearrangements and found no rearrangement; Ishizaka et al. (1991) found one ret/PTC1 rearrangement among 11 carcinomas, and Wajjwalku et al. (1992) found only one such alteration among 38 carcinomas. On the basis of those observations, RET gene rearrang ...
Muscle pattern diversification in Drosophila: the story of
... the mesothorax consists of the large indirect flight muscles (IFMs), which are divisible into two subsets: the dorsal longitudinal muscles (DLMs) and the dorsoventral muscles (DVMs).(25) These two groups of IFMs have distinct developmental histories: the DLMs develop using persistent larval muscles ...
... the mesothorax consists of the large indirect flight muscles (IFMs), which are divisible into two subsets: the dorsal longitudinal muscles (DLMs) and the dorsoventral muscles (DVMs).(25) These two groups of IFMs have distinct developmental histories: the DLMs develop using persistent larval muscles ...
PDF
... The physiological activities of organs are underpinned by an interplay between the distinct cell types they contain. However, little is known about the genetic control of patterned cell differentiation during organ development. We show that the conserved Teashirt transcription factors are decisive f ...
... The physiological activities of organs are underpinned by an interplay between the distinct cell types they contain. However, little is known about the genetic control of patterned cell differentiation during organ development. We show that the conserved Teashirt transcription factors are decisive f ...
Quantitative Genetics
... genetic variation in a given population for a trait. Note that Fig. 20.15 talks about how to measure H2 in humans using twin studies. What one would really like is some quasiexperimental measure of the extent to which we can expect a given trait to vary when we vary the genotype of the organism. I.e ...
... genetic variation in a given population for a trait. Note that Fig. 20.15 talks about how to measure H2 in humans using twin studies. What one would really like is some quasiexperimental measure of the extent to which we can expect a given trait to vary when we vary the genotype of the organism. I.e ...
Problem Sets - MIT Biology
... (g) List all of the above six crosses (parts (a) – (f)) that are proper complementation tests which clearly reveal whether two mutations are in the same gene or different genes. Part e) is a proper complementation test that clearly shows that wild-type alleles of A and B will rescue the mutant pheno ...
... (g) List all of the above six crosses (parts (a) – (f)) that are proper complementation tests which clearly reveal whether two mutations are in the same gene or different genes. Part e) is a proper complementation test that clearly shows that wild-type alleles of A and B will rescue the mutant pheno ...
DNA cytosine methylation in plant development
... 1). In Arabidopsis, establishing methylation in all sequence contexts is entirely dependent on the de novo methyltransferase, DOMAINS REARRANGED METHYLTRANSFERASE (DRM) (similar to the mammalian Dnmt3 family) activity (Cao et al, 2000), which functions through the RNA-directed DNA methylation (RdDM) ...
... 1). In Arabidopsis, establishing methylation in all sequence contexts is entirely dependent on the de novo methyltransferase, DOMAINS REARRANGED METHYLTRANSFERASE (DRM) (similar to the mammalian Dnmt3 family) activity (Cao et al, 2000), which functions through the RNA-directed DNA methylation (RdDM) ...
Detection of genetically modified plants
... 2) Identification. If there is a positive detection of GMOs, further analysis is required to discover which GMO it is and thus whether the GMO is approved within the EU. The only analytical methods, which unequivocally may enable identification of each GMO variety are methods based on PCR. 3) Quanti ...
... 2) Identification. If there is a positive detection of GMOs, further analysis is required to discover which GMO it is and thus whether the GMO is approved within the EU. The only analytical methods, which unequivocally may enable identification of each GMO variety are methods based on PCR. 3) Quanti ...
A Metabolic Node in Action: Chorismate
... that led to the synthesis of an extensive range of products. This pathway is present only in bacteria, fungi, and plants. While there is only little difference in the sequence of the chemical reactions of the pathway, significant differences exist in terms of organization and regulation. In the main ...
... that led to the synthesis of an extensive range of products. This pathway is present only in bacteria, fungi, and plants. While there is only little difference in the sequence of the chemical reactions of the pathway, significant differences exist in terms of organization and regulation. In the main ...
Phylogenetic analysis of three complete gap
... from the zebrafish genome, some are found as single relatives, and others are found in multiple copies. In addition, the zebrafish has 14 apparently novel connexins, several of which arose by recent tandem duplication events. This analysis provides evidence that the connexin gene family is increasin ...
... from the zebrafish genome, some are found as single relatives, and others are found in multiple copies. In addition, the zebrafish has 14 apparently novel connexins, several of which arose by recent tandem duplication events. This analysis provides evidence that the connexin gene family is increasin ...
A TaqI polymorphism in the 3 UTR of the IL-12
... The fact that the TaqI polymorphism in the p40 gene has an effect on IL-12 p70 secretion, but not on IL-12 p40 secretion, is of considerable interest and, at first glance, may appear counterintuitive. One possibility is that the polymorphism affects p40 homodimer formation, which, in turn, influence ...
... The fact that the TaqI polymorphism in the p40 gene has an effect on IL-12 p70 secretion, but not on IL-12 p40 secretion, is of considerable interest and, at first glance, may appear counterintuitive. One possibility is that the polymorphism affects p40 homodimer formation, which, in turn, influence ...
RNA-Seq

RNA-seq (RNA sequencing), also called whole transcriptome shotgun sequencing (WTSS), is a technology that uses the capabilities of next-generation sequencing to reveal a snapshot of RNA presence and quantity from a genome at a given moment in time.