DNA cytosine methylation in plant development
... 1). In Arabidopsis, establishing methylation in all sequence contexts is entirely dependent on the de novo methyltransferase, DOMAINS REARRANGED METHYLTRANSFERASE (DRM) (similar to the mammalian Dnmt3 family) activity (Cao et al, 2000), which functions through the RNA-directed DNA methylation (RdDM) ...
... 1). In Arabidopsis, establishing methylation in all sequence contexts is entirely dependent on the de novo methyltransferase, DOMAINS REARRANGED METHYLTRANSFERASE (DRM) (similar to the mammalian Dnmt3 family) activity (Cao et al, 2000), which functions through the RNA-directed DNA methylation (RdDM) ...
CHK2 kinase: cancer susceptibility and cancer therapy – two sides
... cells delay DNA synthesis and cell division following DNA damage. The original studies that identified human CHK2 demonstrated that it can phosphorylate the CDC25C phosphatase, which is required for the activation of cyclin-dependent kinase (CDK) complexes that regulate cell-cycle progression. Phosp ...
... cells delay DNA synthesis and cell division following DNA damage. The original studies that identified human CHK2 demonstrated that it can phosphorylate the CDC25C phosphatase, which is required for the activation of cyclin-dependent kinase (CDK) complexes that regulate cell-cycle progression. Phosp ...
Mutations affecting craniofacial development in zebrafish
... Genetic analysis Genetic segregation of individual mutations was determined by assessing on average 1000 embryos from crosses of heterozygous parents for any given mutation. In order to test potential allelism of isolated mutations, complementation testing among members of the phenotypically defined ...
... Genetic analysis Genetic segregation of individual mutations was determined by assessing on average 1000 embryos from crosses of heterozygous parents for any given mutation. In order to test potential allelism of isolated mutations, complementation testing among members of the phenotypically defined ...
the lkb1 tumor suppressor - E
... maintaining genomic integrity. This class of tumor suppressors contains genes involved in DNA repair and replication. Mutations in caretaker genes result in genomic instability and in increased mutation rate. Consequently, gatekeepers are prone to become inactivated and oncogenes activated due to th ...
... maintaining genomic integrity. This class of tumor suppressors contains genes involved in DNA repair and replication. Mutations in caretaker genes result in genomic instability and in increased mutation rate. Consequently, gatekeepers are prone to become inactivated and oncogenes activated due to th ...
Molecular identification of three Arabidopsis thaliana mitochondrial
... Total RNA, isolated from various tissues of A. thaliana by using TRIzol® (Life Technologies), was reverse-transcribed with the GeneAmp RNA PCR Core kit (Applied Biosystems) with random hexamers as primers. The integrity of the starting RNA was verified by gel electrophoresis. Primers and probes base ...
... Total RNA, isolated from various tissues of A. thaliana by using TRIzol® (Life Technologies), was reverse-transcribed with the GeneAmp RNA PCR Core kit (Applied Biosystems) with random hexamers as primers. The integrity of the starting RNA was verified by gel electrophoresis. Primers and probes base ...
Title: Statistical Evidence for Common Ancestry
... GC content across the genome (Plotkin and Kudla 2011), which need to be taken into account in our null model. We developed three different expected codon usage distributions at invariant amino acid positions under SA (see Materials and Methods). We also modified the tests to mask positions in which ...
... GC content across the genome (Plotkin and Kudla 2011), which need to be taken into account in our null model. We developed three different expected codon usage distributions at invariant amino acid positions under SA (see Materials and Methods). We also modified the tests to mask positions in which ...
lecture - Berkeley MCB
... exogenous inducer displaces this repressor and restores enzyme synthesis. With the i- allele, present in an inactive form in the constitutives, the repressor is not formed, and b-galactosidase is synthesized, the exogenous inducer therefore being without effect. This hypothesis, although at first su ...
... exogenous inducer displaces this repressor and restores enzyme synthesis. With the i- allele, present in an inactive form in the constitutives, the repressor is not formed, and b-galactosidase is synthesized, the exogenous inducer therefore being without effect. This hypothesis, although at first su ...
CFTR Mutations in Congenital Absence of Vas Deferens
... explained by another genetic factor, namely the polymorphic TGm locus in front of the Tn locus (11). Different alleles can be found depending on the number of TG repeats found at this locus. The higher number of TG repeats, the less efficient exon 9 splicing will occur (Fig 2). The 5T polymorphism c ...
... explained by another genetic factor, namely the polymorphic TGm locus in front of the Tn locus (11). Different alleles can be found depending on the number of TG repeats found at this locus. The higher number of TG repeats, the less efficient exon 9 splicing will occur (Fig 2). The 5T polymorphism c ...
Acetate kinase from CIostridiurn acetobutylicurn : a highly specific
... Affinity chromatography: About 10 mg protein (in 2 ml) was loaded onto a C 10/20 column containing 8 ml Procion Blue HE3B (Pharmacia) equilibrated with imidazole buffer. The column was washed with 40 ml of the same buffer, and the enzyme was eluted by an ATP gradient (0-1 mM ATP in imidazole buffer; ...
... Affinity chromatography: About 10 mg protein (in 2 ml) was loaded onto a C 10/20 column containing 8 ml Procion Blue HE3B (Pharmacia) equilibrated with imidazole buffer. The column was washed with 40 ml of the same buffer, and the enzyme was eluted by an ATP gradient (0-1 mM ATP in imidazole buffer; ...
Making Headway: The Roles of Hox Genes and Neural
... hindbrain, however little is known of the cascade of events that regulate their expression or the process of segmentation itself. In general, Hox gene expression patterns are generated in two distinct phases: establishment followed by maintenance. The mechanism by which Hox genes become expressed at ...
... hindbrain, however little is known of the cascade of events that regulate their expression or the process of segmentation itself. In general, Hox gene expression patterns are generated in two distinct phases: establishment followed by maintenance. The mechanism by which Hox genes become expressed at ...
CONSERVATION AND DIVERGENCE IN MOLECULAR
... and thereby freeing Dpp to function independently of Sog. The isolation of interacting antimorphic mutations was used to suggest a physical interaction between the tolloid and dpp gene products. These phenotypes have not been reconciled with the current view that tolloid acts on sog (24, 40, 41, 123 ...
... and thereby freeing Dpp to function independently of Sog. The isolation of interacting antimorphic mutations was used to suggest a physical interaction between the tolloid and dpp gene products. These phenotypes have not been reconciled with the current view that tolloid acts on sog (24, 40, 41, 123 ...
A Cladistic Analysis of Phenotype Associations with
... mapping) with phenotypic variation at the population level. We confine our analyses to DNA regions in which recombination is very rare. In this case, the genetic variation at the candiate locus can be organized into a cladogram that represents the evolutionary relationships between the observed hapl ...
... mapping) with phenotypic variation at the population level. We confine our analyses to DNA regions in which recombination is very rare. In this case, the genetic variation at the candiate locus can be organized into a cladogram that represents the evolutionary relationships between the observed hapl ...
gsea user guide
... dataset. For example, before running hierarchical clustering, you might remove genes that have low variance across the dataset. This prevents flat genes from driving the clustering result and improves processing time by focusing on a smaller number of interesting genes. The GSEA algorithm does not f ...
... dataset. For example, before running hierarchical clustering, you might remove genes that have low variance across the dataset. This prevents flat genes from driving the clustering result and improves processing time by focusing on a smaller number of interesting genes. The GSEA algorithm does not f ...
1 shared allele
... The paradigm under which Galton and other “biometricians” worked was that inheritance of human traits involved the mixing or blending of factors present in the parents. This picture is very different from that obtained from Mendel’s experiments, which implied that inherited traits are determined by ...
... The paradigm under which Galton and other “biometricians” worked was that inheritance of human traits involved the mixing or blending of factors present in the parents. This picture is very different from that obtained from Mendel’s experiments, which implied that inherited traits are determined by ...
Function and Control of the Spx-Family of Proteins Within
... bacterium, Bacillus subtilis is especially complex, involving three forms of RNA polymerase and at least two transcriptional repressors. Signal transduction systems connected to cell envelope stress transcriptionally control spx genes in orthologous Gram-positive systems. High levels of Spx not only ...
... bacterium, Bacillus subtilis is especially complex, involving three forms of RNA polymerase and at least two transcriptional repressors. Signal transduction systems connected to cell envelope stress transcriptionally control spx genes in orthologous Gram-positive systems. High levels of Spx not only ...
Prader-Willi and Angelman syndromes: Sister imprinted disorders
... to chromosome 15q11-q13. They both have characteristic neurologic, developmental, and behavioral phenotypes plus other structural and functional abnormalities. However, the cognitive and neurologic impairment is more severe in AS, including seizures and ataxia. The behavioral and endocrine disorders ...
... to chromosome 15q11-q13. They both have characteristic neurologic, developmental, and behavioral phenotypes plus other structural and functional abnormalities. However, the cognitive and neurologic impairment is more severe in AS, including seizures and ataxia. The behavioral and endocrine disorders ...
lecture
... informed about the new carrier tests. At the time of screening, patients could choose their tests (hexosaminidase A by enzyme analysis for Tay-Sachs disease or mutation analysis for cystic fibrosis and Gaucher disease). … The majority of Ashkenazi Jewish patients chose to have testing for all 3 dise ...
... informed about the new carrier tests. At the time of screening, patients could choose their tests (hexosaminidase A by enzyme analysis for Tay-Sachs disease or mutation analysis for cystic fibrosis and Gaucher disease). … The majority of Ashkenazi Jewish patients chose to have testing for all 3 dise ...
Ovarian surface epithelium: family history and early events in
... groups have some limitations. First, it is difficult to accurately define the risk of ovarian cancer. Women in the general population who are carriers of BRCA mutations may be at a greater risk for ovarian cancer due to genetic reasons, however this does not account for other known nongenetic factor ...
... groups have some limitations. First, it is difficult to accurately define the risk of ovarian cancer. Women in the general population who are carriers of BRCA mutations may be at a greater risk for ovarian cancer due to genetic reasons, however this does not account for other known nongenetic factor ...
Math of Genetics - College of William & Mary
... Pepper color is controlled by two different genes The first gene controls the expression of red pigment The dominant allele (R) indicates the presence of red ...
... Pepper color is controlled by two different genes The first gene controls the expression of red pigment The dominant allele (R) indicates the presence of red ...
MOLECULAR BIOLOGY OF FRUIT MATURATION AND
... tomato, cell wall metabolism in particular, and the effects of the gaseous hormone ethylene (19, 46, 48, 52, 81, 161). The ripe phenotype is the summation of biochemical and physiological changes that occur at the terminal stage of fruit development and render the organ edible and desirable to seed- ...
... tomato, cell wall metabolism in particular, and the effects of the gaseous hormone ethylene (19, 46, 48, 52, 81, 161). The ripe phenotype is the summation of biochemical and physiological changes that occur at the terminal stage of fruit development and render the organ edible and desirable to seed- ...
Chapter 4 - DORAS
... indicated that heam uptake was occurring at wild type levels (Ó Cuív, PhD Thesis 2003). Mutations in each of the genes hmuTUV were subsequently created in an rhtX negative background and displayed a reduced level of haem uptake compared to wild type (Unpublished observations). The improved clarity i ...
... indicated that heam uptake was occurring at wild type levels (Ó Cuív, PhD Thesis 2003). Mutations in each of the genes hmuTUV were subsequently created in an rhtX negative background and displayed a reduced level of haem uptake compared to wild type (Unpublished observations). The improved clarity i ...
1 Article: Investigation Evidence for Stabilizing Selection on Codon
... mutations. This gene arrangement polymorphism has been stable in natural populations for over seventy years (Figure 1A; Dobzhansky and Sturtevant 1938). Frequencies of particular chromosome arrangements form a classical cline across the species range in the American southwest despite the predicted e ...
... mutations. This gene arrangement polymorphism has been stable in natural populations for over seventy years (Figure 1A; Dobzhansky and Sturtevant 1938). Frequencies of particular chromosome arrangements form a classical cline across the species range in the American southwest despite the predicted e ...
Note for Guidance on the Quality, Preclinical and Clinical
... The design of the gene transfer product forms an integral part of the rationale for the development of a gene transfer medicinal product. Information relating to the suitability of the vector system and of the delivery system should be provided. Data on the control and stability of gene expression s ...
... The design of the gene transfer product forms an integral part of the rationale for the development of a gene transfer medicinal product. Information relating to the suitability of the vector system and of the delivery system should be provided. Data on the control and stability of gene expression s ...
RNA-Seq
RNA-seq (RNA sequencing), also called whole transcriptome shotgun sequencing (WTSS), is a technology that uses the capabilities of next-generation sequencing to reveal a snapshot of RNA presence and quantity from a genome at a given moment in time.