pKa of adenine 2451 in the ribosomal peptidyl transferase center
... We recently showed that the mutation of A2451 in Thermus aquaticus 23S rRNA only moderately affected the peptidyl transferase activity of in vitro assembled 50S ribosomal subunits (Polacek et al+, 2001)+ These results were further supported by similar in vitro as well as in vivo experiments that uti ...
... We recently showed that the mutation of A2451 in Thermus aquaticus 23S rRNA only moderately affected the peptidyl transferase activity of in vitro assembled 50S ribosomal subunits (Polacek et al+, 2001)+ These results were further supported by similar in vitro as well as in vivo experiments that uti ...
The DUET gene is necessary for chromosome
... parent. F1 and F2 were selected on MS plates containing kanamycin at 50 µg/ml. 96 F2 plants were transferred to soil; 49 of these were sterile. Plants homozygous for dyad were identified by examination of ovules as described previously (Agashe et al., 2002). The presence of the insertion and wild-ty ...
... parent. F1 and F2 were selected on MS plates containing kanamycin at 50 µg/ml. 96 F2 plants were transferred to soil; 49 of these were sterile. Plants homozygous for dyad were identified by examination of ovules as described previously (Agashe et al., 2002). The presence of the insertion and wild-ty ...
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... CGG repeats (28,29). However, the length of CGG repeats on a particular allele in a differentiated cell derived from these carriers is generally mitotically stable although recent studies show that fully expanded FMR1 CGG repeats exhibit a lengthand differentiation-dependent instability in cell hybr ...
... CGG repeats (28,29). However, the length of CGG repeats on a particular allele in a differentiated cell derived from these carriers is generally mitotically stable although recent studies show that fully expanded FMR1 CGG repeats exhibit a lengthand differentiation-dependent instability in cell hybr ...
Clinical Features, and Nucleotide Changes in Three Families with
... muscle palsy was based on the presence of hypertropia (upward deviation of the eye) on the affected paralyzed side, which became greatest in the gaze toward the nasal field of the involved eye. The additional diagnostic feature was a positive Bielschowsky head tilt test, showing an upward deviation of ...
... muscle palsy was based on the presence of hypertropia (upward deviation of the eye) on the affected paralyzed side, which became greatest in the gaze toward the nasal field of the involved eye. The additional diagnostic feature was a positive Bielschowsky head tilt test, showing an upward deviation of ...
Benchmarking two commonly used Saccharomyces
... strain (Otero et al., 2010; Nijkamp et al., 2012). For example, the sequencing comparison studies revealed that 83 genes were absent in CEN.PK relative to S288C (Nijkamp et al., 2012) and that more than 22,000 single nucleotide polymorphisms (SNPs) exist between the two strains. 13,000 of the SNPs a ...
... strain (Otero et al., 2010; Nijkamp et al., 2012). For example, the sequencing comparison studies revealed that 83 genes were absent in CEN.PK relative to S288C (Nijkamp et al., 2012) and that more than 22,000 single nucleotide polymorphisms (SNPs) exist between the two strains. 13,000 of the SNPs a ...
Cytoplasmic male sterility
... 79BS14-3 is a carrier of a recessive or semidominant restorer gene. The Fa seed was produced in bagged F1 spikes. Seventy-one accessions of spontaneum barleys have been tested for restoration both in m s m l and msm2 cytoplasms with an Adorra-like seed parent background produced by repeated backcros ...
... 79BS14-3 is a carrier of a recessive or semidominant restorer gene. The Fa seed was produced in bagged F1 spikes. Seventy-one accessions of spontaneum barleys have been tested for restoration both in m s m l and msm2 cytoplasms with an Adorra-like seed parent background produced by repeated backcros ...
Package `omicade4`
... Two functions could be used to visualize the result of mcia: The first is plot.mcia, which results in four plots. Top left represents the sample space. Individuals from the same column of different data.frames are linked by edges. Different platforms are distinguished by the shape of points. Top rig ...
... Two functions could be used to visualize the result of mcia: The first is plot.mcia, which results in four plots. Top left represents the sample space. Individuals from the same column of different data.frames are linked by edges. Different platforms are distinguished by the shape of points. Top rig ...
Lecture10
... Critical Genetic Link Found Between Human Taste Differences And Nicotine Dependence http://www.sciencedaily.com/releases/2008/10/081014111301.htm ...
... Critical Genetic Link Found Between Human Taste Differences And Nicotine Dependence http://www.sciencedaily.com/releases/2008/10/081014111301.htm ...
The XTH Family of Enzymes Involved in Xyloglucan
... The 33 XTH genes are dispersed across all five chromosomes of Arabidopsis, with one third of the genes occurring as clusters consisting of two to four members (Fig. 2A and Yokoyama and Nishitani 2001b), probably resulting from genome duplication and gene reshuffling (Blanc et al. 2000). For example, ...
... The 33 XTH genes are dispersed across all five chromosomes of Arabidopsis, with one third of the genes occurring as clusters consisting of two to four members (Fig. 2A and Yokoyama and Nishitani 2001b), probably resulting from genome duplication and gene reshuffling (Blanc et al. 2000). For example, ...
Higher order structural effects stabilizing the
... we decided to extend the analysis to search for other environmental factors that could act similarly. We started with a thorough search of the wwPDB (37), looking for occurrences of the G:C W:W trans pairs involved in H-bonding interactions with other bases, the ribose-phosphate backbone and water m ...
... we decided to extend the analysis to search for other environmental factors that could act similarly. We started with a thorough search of the wwPDB (37), looking for occurrences of the G:C W:W trans pairs involved in H-bonding interactions with other bases, the ribose-phosphate backbone and water m ...
A novel locus of Yersinia enterocolitica serotype O:3 involved in
... in YeO3-RfbR12 and are shown in Fig. 1. Transcomplementation of YeO3-RfbR12, YeO3-trs11 and YeO3-RfbR14 (the last strain was included to see if the basis of its phage resistance was similar to that of YeO3R12) was monitored using phage ~R1-37 (Table 2). However, trans-complementation was not straigh ...
... in YeO3-RfbR12 and are shown in Fig. 1. Transcomplementation of YeO3-RfbR12, YeO3-trs11 and YeO3-RfbR14 (the last strain was included to see if the basis of its phage resistance was similar to that of YeO3R12) was monitored using phage ~R1-37 (Table 2). However, trans-complementation was not straigh ...
Genome Jigsaw: Implications of 16S Ribosomal RNA Gene
... purposes. Current methodologies for characterizing microbial communities, such as those used to study the human microbiome, involve sequencing short fragments of this ubiquitous gene, and comparing these fragments to reference sequences in databases to identify the microbes present. Traditionally, w ...
... purposes. Current methodologies for characterizing microbial communities, such as those used to study the human microbiome, involve sequencing short fragments of this ubiquitous gene, and comparing these fragments to reference sequences in databases to identify the microbes present. Traditionally, w ...
Looking for a strong promoter for Physcomitrella
... However, in the moss Physcomitrella patens, its promoter strength is weak or all but silent in the dark.1 Therefore, it is necessary to find promoters that show higher expression levels and that have a more ubiquitous expression over time, tissues and treatments. Of further interest are cell or tiss ...
... However, in the moss Physcomitrella patens, its promoter strength is weak or all but silent in the dark.1 Therefore, it is necessary to find promoters that show higher expression levels and that have a more ubiquitous expression over time, tissues and treatments. Of further interest are cell or tiss ...
Evolutionary dynamics of nematode operons
... It has been estimated that the separation between C. elegans and C. briggsae occurred T = 80 to 110 million years ago (Mya) (Coghlan 2003; Stein et al. 2003; Hillier et al. 2007). Thus, the rate of operon formation is Rgain = 56/T = 0.51–0.70 operons per million years (Myr). Of the 1133 C. elegans o ...
... It has been estimated that the separation between C. elegans and C. briggsae occurred T = 80 to 110 million years ago (Mya) (Coghlan 2003; Stein et al. 2003; Hillier et al. 2007). Thus, the rate of operon formation is Rgain = 56/T = 0.51–0.70 operons per million years (Myr). Of the 1133 C. elegans o ...
redundancy and regulatory interactions between two
... (AF052570), suggesting a possible role for this sequence in the negative regulation of C-function gene expression (Figure 1B). Two types of FAR cDNA were identified in the cDNA library at different frequencies. The DNA sequence and predicted amino acid-coding potential of the rarer of the two cDNAs ...
... (AF052570), suggesting a possible role for this sequence in the negative regulation of C-function gene expression (Figure 1B). Two types of FAR cDNA were identified in the cDNA library at different frequencies. The DNA sequence and predicted amino acid-coding potential of the rarer of the two cDNAs ...
Transcriptomic and Reverse Genetic Analyses of Branched
... interesting questions of substrate specificity and the need to identify specific genes. Furthermore, evidence to date has been based primarily on isotopic labeling studies with potential caveats indicated in the literature (van der Hoeven and Steffens, 2000; Kroumova and Wagner, 2003). This emphasiz ...
... interesting questions of substrate specificity and the need to identify specific genes. Furthermore, evidence to date has been based primarily on isotopic labeling studies with potential caveats indicated in the literature (van der Hoeven and Steffens, 2000; Kroumova and Wagner, 2003). This emphasiz ...
draft - IC
... When more than two genomes are considered, we have the more challenging problem of rearrangement-based phylogeny reconstruction, where we want to nd a tree that minimizes the total number of rearrangement events. Early approaches were based on a breakpoint distance (e.g., BPAnalysis [13], and GRAPP ...
... When more than two genomes are considered, we have the more challenging problem of rearrangement-based phylogeny reconstruction, where we want to nd a tree that minimizes the total number of rearrangement events. Early approaches were based on a breakpoint distance (e.g., BPAnalysis [13], and GRAPP ...
Role of two-component systems in the virulence of Streptococcus
... key mechanism through which bacteria perceive and respond to their environment. Here we discuss the significant advances that have been made in the understanding of TCSs in Streptococcus pneumoniae (the pneumococcus), in particular with regards to the role of these systems in the virulence of this i ...
... key mechanism through which bacteria perceive and respond to their environment. Here we discuss the significant advances that have been made in the understanding of TCSs in Streptococcus pneumoniae (the pneumococcus), in particular with regards to the role of these systems in the virulence of this i ...
Deconstructing the Genome: DNA at High Resolution
... cloning, for example, has produced, among many other medically useful products, human insulin for diabetics, which provokes far fewer allergic reactions than the pig insulin that preceded it; tissue plasminogen activator (TPA), a protein that helps dissolve blood clots; and gamma interferon, a prote ...
... cloning, for example, has produced, among many other medically useful products, human insulin for diabetics, which provokes far fewer allergic reactions than the pig insulin that preceded it; tissue plasminogen activator (TPA), a protein that helps dissolve blood clots; and gamma interferon, a prote ...
Two glucose/xylose transporter genes from the yeast Candida
... different concentrations of either xylose or glucose as the sole carbon and energy source. Approx. 40 transformants that were able to grow well on glucose, although they grew weakly on xylose, were selected. None of the transformants exhibited proton symport activity, as determined by the absence of ...
... different concentrations of either xylose or glucose as the sole carbon and energy source. Approx. 40 transformants that were able to grow well on glucose, although they grew weakly on xylose, were selected. None of the transformants exhibited proton symport activity, as determined by the absence of ...
Supplementary Table 1 Entrez Gene (http://www.ncbi.nlm.nih.gov
... (XLMR) syndrome most often accompanied by alpha-thalassemia (ATRX) syndrome. These mutations have been shown to cause diverse changes in the pattern of DNA methylation, which may provide a link between chromatin remodeling, DNA methylation, and gene expression in developmental processes. Multiple al ...
... (XLMR) syndrome most often accompanied by alpha-thalassemia (ATRX) syndrome. These mutations have been shown to cause diverse changes in the pattern of DNA methylation, which may provide a link between chromatin remodeling, DNA methylation, and gene expression in developmental processes. Multiple al ...
Evolution and selection of trichromatic vision in primates
... known as PHOTOPIGMENTS , which differ in the wavelength of light to which they are sensitive [8]. This is known as their ‘SPECTRAL SENSITIVITY ’. An increased number of photopigments, with different sensitivities, enables a greater number of comparisons to be made and, hence, increases the potential ...
... known as PHOTOPIGMENTS , which differ in the wavelength of light to which they are sensitive [8]. This is known as their ‘SPECTRAL SENSITIVITY ’. An increased number of photopigments, with different sensitivities, enables a greater number of comparisons to be made and, hence, increases the potential ...
Alternative mRNA Splicing Generates the Two
... the first 22 nucleotides of the intervening sequence were retained in the mature rubisco activase mRNA. The 22nucleotide auxiliary exon contains an in-frame ochre termination codon and leads to the synthesis of a 41-kilodalton polypeptide. Removal of the entire 137-nucleotide intervening sequence re ...
... the first 22 nucleotides of the intervening sequence were retained in the mature rubisco activase mRNA. The 22nucleotide auxiliary exon contains an in-frame ochre termination codon and leads to the synthesis of a 41-kilodalton polypeptide. Removal of the entire 137-nucleotide intervening sequence re ...
RNA-Seq
RNA-seq (RNA sequencing), also called whole transcriptome shotgun sequencing (WTSS), is a technology that uses the capabilities of next-generation sequencing to reveal a snapshot of RNA presence and quantity from a genome at a given moment in time.