Taxonomic evaluation of the Streptomyces griseus clade using
... PAUP and PHYLIP, respectively; asterisks indicate branches that were supported by all three tree-making algorithms. Bars, 1 % difference in nucleotide sequence. ...
... PAUP and PHYLIP, respectively; asterisks indicate branches that were supported by all three tree-making algorithms. Bars, 1 % difference in nucleotide sequence. ...
Arsenite as an Electron Donor for Anoxygenic Photosynthesis
... The PCR products were cloned using the Invitrogen TA-TOPO cloning kit according to the manufacturer’s instructions. Clones were screened for correctly sized inserts (~1500 bp). Sequencing was done on replicate clones by Sequetech DNA Sequencing Service (Mountain View, CA, USA) with forward (M13F) an ...
... The PCR products were cloned using the Invitrogen TA-TOPO cloning kit according to the manufacturer’s instructions. Clones were screened for correctly sized inserts (~1500 bp). Sequencing was done on replicate clones by Sequetech DNA Sequencing Service (Mountain View, CA, USA) with forward (M13F) an ...
Tumour necrosis factor and lymphotoxin A progression? EDITORIAL
... among them is one of the authors’ own studies that included only two polymorphisms in the genes [2]. In the present study, the authors analyse a sample that is sufficiently large to exclude potential negative findings due to small study populations, and thus lack of power. Indeed, one of the strengt ...
... among them is one of the authors’ own studies that included only two polymorphisms in the genes [2]. In the present study, the authors analyse a sample that is sufficiently large to exclude potential negative findings due to small study populations, and thus lack of power. Indeed, one of the strengt ...
Metatranscriptome_Pipeline_Tutorial.doc
... generated by the sequencer to files that can be read by the visualization tool, here we illustrate the use of the pipeline by going through the various steps to illustrate the complexity of the process and the underlying tools and files used and generated by the pipeline. To illustrate the process w ...
... generated by the sequencer to files that can be read by the visualization tool, here we illustrate the use of the pipeline by going through the various steps to illustrate the complexity of the process and the underlying tools and files used and generated by the pipeline. To illustrate the process w ...
6.
... to how many of these predicted splice variants are functional and how many are the result of aberrant splicing (or ‘noise’). To address this question, we compared alternatively spliced cassette exons that are conserved between human and mouse with EST-predicted cassette exons that are not conserved ...
... to how many of these predicted splice variants are functional and how many are the result of aberrant splicing (or ‘noise’). To address this question, we compared alternatively spliced cassette exons that are conserved between human and mouse with EST-predicted cassette exons that are not conserved ...
Bioinfo primer - part 4/6
... the matrix from top left to bottom right. The best-scoring path can be traced through the matrix, resulting in an optimal alignment. The NeedlemanWunsch algorithm belongs to this class. • Sequences are often modular, therefore similarities can be only local and global alignments will fail. The Smith ...
... the matrix from top left to bottom right. The best-scoring path can be traced through the matrix, resulting in an optimal alignment. The NeedlemanWunsch algorithm belongs to this class. • Sequences are often modular, therefore similarities can be only local and global alignments will fail. The Smith ...
Leukaemia Section ider(20q) in Myeloid Malignancies Atlas of Genetics and Cytogenetics
... Leukemia (5 cases), Chronic Myelomonocytic Leukemia (1 case). ...
... Leukemia (5 cases), Chronic Myelomonocytic Leukemia (1 case). ...
Functional characterization of polypeptide release factor 1b in the
... in yeast cells (Figure 4a). This suggests that hybrid eRF1 cannot recognize all three stop codons UAA, UAG and UGA to correctly complete the termination process of protein biosynthesis. The read-through assay was performed to address why Eob/Sc eRF1 could not support the viability of the above yeast ...
... in yeast cells (Figure 4a). This suggests that hybrid eRF1 cannot recognize all three stop codons UAA, UAG and UGA to correctly complete the termination process of protein biosynthesis. The read-through assay was performed to address why Eob/Sc eRF1 could not support the viability of the above yeast ...
C. neoformans
... N2 nematodes were fed on lawns of C. neoformans and then at different time-points transferred to lawns of non-pathogenic cryptococci. All nematodes that survived transfer were rescued, regained normal non-distended anatomy and no C. neoformans yeast cells could be cultured followed “grinding” of the ...
... N2 nematodes were fed on lawns of C. neoformans and then at different time-points transferred to lawns of non-pathogenic cryptococci. All nematodes that survived transfer were rescued, regained normal non-distended anatomy and no C. neoformans yeast cells could be cultured followed “grinding” of the ...
Convergent Evolution in the Genetic Basis of Müllerian Mimicry in
... brood 44, and where possible, broods 48 and 52. Genotyping was performed using (i) size variation in PCR products when separated using agarose gel electrophoresis or (ii) sequencing F1 parents from crosses to identify restriction enzymes that recognize single nucleotide polymorphisms segregating in ...
... brood 44, and where possible, broods 48 and 52. Genotyping was performed using (i) size variation in PCR products when separated using agarose gel electrophoresis or (ii) sequencing F1 parents from crosses to identify restriction enzymes that recognize single nucleotide polymorphisms segregating in ...
PH4 of Petunia Is an R2R3 MYB Protein That Activates
... derived lines) and the 3-rutinosides show little or no fading (de Vlaming et al., 1982). When we crossed the unstable ph4-V2166 allele into a genetic background that allows the synthesis of 3RGac5G-substituted anthocyanins, the flowers displayed upon opening a blue-violet color and were dotted with ...
... derived lines) and the 3-rutinosides show little or no fading (de Vlaming et al., 1982). When we crossed the unstable ph4-V2166 allele into a genetic background that allows the synthesis of 3RGac5G-substituted anthocyanins, the flowers displayed upon opening a blue-violet color and were dotted with ...
Convergent Evolution in the Genetic Basis of Müllerian
... brood 44, and where possible, broods 48 and 52. Genotyping was performed using (i) size variation in PCR products when separated using agarose gel electrophoresis or (ii) sequencing F1 parents from crosses to identify restriction enzymes that recognize single nucleotide polymorphisms segregating in ...
... brood 44, and where possible, broods 48 and 52. Genotyping was performed using (i) size variation in PCR products when separated using agarose gel electrophoresis or (ii) sequencing F1 parents from crosses to identify restriction enzymes that recognize single nucleotide polymorphisms segregating in ...
PDF
... (Hanisch et al., 2013; Schroter et al., 2012; Trofka et al., 2012). Furthermore, these models focused on simulating the system only in a wild-type background and were therefore largely unconstrained. More rigorous models can be built by constraining them to explain the spatiotemporal dynamics of the ...
... (Hanisch et al., 2013; Schroter et al., 2012; Trofka et al., 2012). Furthermore, these models focused on simulating the system only in a wild-type background and were therefore largely unconstrained. More rigorous models can be built by constraining them to explain the spatiotemporal dynamics of the ...
Planta
... Fall 1995). One explanation for the lower activity might be that the native protein has been isolated as a heterodimer with slightly dierent subunits (Silver and Fall 1995) whereas the recombinant protein consists only of one polypeptide. Moreover, keeping in mind that the recombinant protein has s ...
... Fall 1995). One explanation for the lower activity might be that the native protein has been isolated as a heterodimer with slightly dierent subunits (Silver and Fall 1995) whereas the recombinant protein consists only of one polypeptide. Moreover, keeping in mind that the recombinant protein has s ...
SLOs - 3.3 Genetics small - Miss Jan`s Science Wikispace
... State some examples of mutagenic agents Explain how these mutagenic agents cause mutations 3. To learn about the effects of mutations Explain why somatic mutations have less significant impact on a species than those that occur in gametes Explain why most mutations are not expressed Explai ...
... State some examples of mutagenic agents Explain how these mutagenic agents cause mutations 3. To learn about the effects of mutations Explain why somatic mutations have less significant impact on a species than those that occur in gametes Explain why most mutations are not expressed Explai ...
Molecular Diagnostics in Clinical Microbiology
... In the last two decades, strategies based on nucleic acid amplification techniques (NAATs) have taken an irreversible position in the diagnostic field of infectious diseases. Pathogens can be detected in qualitative and quantitative NAAT strategies by selection of species-specific nucleic acid targe ...
... In the last two decades, strategies based on nucleic acid amplification techniques (NAATs) have taken an irreversible position in the diagnostic field of infectious diseases. Pathogens can be detected in qualitative and quantitative NAAT strategies by selection of species-specific nucleic acid targe ...
Systematic Studies on the Family Cucurbitaceae
... dotted (24). In addition, seeds may have lighter or darker margins (ring), or may be covered by an additional layer of fleshy pericarp in Egusi cultivars, as controlled by the eg gene (Fig. 1) (12, 13). The genes r, t, and w determine seed color. Black is given by triple dominant; mottled is homozyg ...
... dotted (24). In addition, seeds may have lighter or darker margins (ring), or may be covered by an additional layer of fleshy pericarp in Egusi cultivars, as controlled by the eg gene (Fig. 1) (12, 13). The genes r, t, and w determine seed color. Black is given by triple dominant; mottled is homozyg ...
Probable presence of an ubiquitous cryptic mitochondrial gene on
... mitochondrial genes, such as atp6 and atp8 [3], these genes are either encoded in the nucleus (as it has been shown for the atp6 gene of a non-metazoan (Chlamydomonas reinhardti) [4]) or have been lost, which implies that their function has become dispensable or has been assumed by other proteins. I ...
... mitochondrial genes, such as atp6 and atp8 [3], these genes are either encoded in the nucleus (as it has been shown for the atp6 gene of a non-metazoan (Chlamydomonas reinhardti) [4]) or have been lost, which implies that their function has become dispensable or has been assumed by other proteins. I ...
Chromatin DNA Methylayion
... Increased MBD2/demethylase is responsible for global hypomethylation and maintaining tumor invasion genes hypomethylated and active ...
... Increased MBD2/demethylase is responsible for global hypomethylation and maintaining tumor invasion genes hypomethylated and active ...
Journal of Bacteriology
... Phase variation is a process of reversible, high-frequency phenotypic switching that is mediated by mutation, reorganization, or modification of DNA. This process is used by several bacterial species to generate population diversity that increases bacterial fitness and is important in niche adaptati ...
... Phase variation is a process of reversible, high-frequency phenotypic switching that is mediated by mutation, reorganization, or modification of DNA. This process is used by several bacterial species to generate population diversity that increases bacterial fitness and is important in niche adaptati ...
1. Introduction
... has simply three integration Modules: Integrate Genechips according to genomes and chromosomes; Integrate Genechips according to specific genes and SNPs; Integrate HapMap genotypes. After integration, you can export the integrated data into various formats for statistical genetic analyses. Some basi ...
... has simply three integration Modules: Integrate Genechips according to genomes and chromosomes; Integrate Genechips according to specific genes and SNPs; Integrate HapMap genotypes. After integration, you can export the integrated data into various formats for statistical genetic analyses. Some basi ...
Mutant selection and phenotypic and genetic characterization of
... and denoted as E50A for adaptation using Avicel and E50C for adaptation using cellobiose. Isolation of single colonies Agar (Fisher Scientific, Pittsburgh, PA, USA) solution (18.75 g/L) was prepared, and 40 mL was distributed into each of eight 125-mL serum bottles (Wheaton, Millville, NJ, USA). The ...
... and denoted as E50A for adaptation using Avicel and E50C for adaptation using cellobiose. Isolation of single colonies Agar (Fisher Scientific, Pittsburgh, PA, USA) solution (18.75 g/L) was prepared, and 40 mL was distributed into each of eight 125-mL serum bottles (Wheaton, Millville, NJ, USA). The ...
Gene Section USP1 (ubiquitin specific peptidase 1) Atlas of Genetics and Cytogenetics
... USP1 transcription is controlled by different mechanisms. On one hand, USP1 mRNA levels fluctuate during the cell cycle, reaching a peak in S phase, and remaining low before and after it (Nijman et al., 2005). On the other hand, DNA damaging agents can repress USP1 transcription by a mechanism that ...
... USP1 transcription is controlled by different mechanisms. On one hand, USP1 mRNA levels fluctuate during the cell cycle, reaching a peak in S phase, and remaining low before and after it (Nijman et al., 2005). On the other hand, DNA damaging agents can repress USP1 transcription by a mechanism that ...
SERIES ‘‘GENETICS OF ASTHMA AND COPD IN THE POSTGENOME ERA’’
... airway compartments that this and other key inflammatory pathways interact with that are important in disease expression, rather than focussing on whether cells are simply present or not. For example, mast cells present in smooth muscle are closely related to BHR [22], whereas eosinophils measured i ...
... airway compartments that this and other key inflammatory pathways interact with that are important in disease expression, rather than focussing on whether cells are simply present or not. For example, mast cells present in smooth muscle are closely related to BHR [22], whereas eosinophils measured i ...
Tricarboxylic Acid Cycle-Dependent Regulation of Staphylococcus
... membrane was incubated for 2 h with PIA-specific antiserum (generously provided by Michael Otto), which was followed by a 2-h incubation with an antirabbit immunoglobulin G peroxidase conjugate. The presence of PIA was detected using SuperSignal West Pico chemiluminescent substrate (Pierce). The int ...
... membrane was incubated for 2 h with PIA-specific antiserum (generously provided by Michael Otto), which was followed by a 2-h incubation with an antirabbit immunoglobulin G peroxidase conjugate. The presence of PIA was detected using SuperSignal West Pico chemiluminescent substrate (Pierce). The int ...
RNA-Seq
RNA-seq (RNA sequencing), also called whole transcriptome shotgun sequencing (WTSS), is a technology that uses the capabilities of next-generation sequencing to reveal a snapshot of RNA presence and quantity from a genome at a given moment in time.