
Tricarboxylic Acid Cycle-Dependent Regulation of Staphylococcus
... membrane was incubated for 2 h with PIA-specific antiserum (generously provided by Michael Otto), which was followed by a 2-h incubation with an antirabbit immunoglobulin G peroxidase conjugate. The presence of PIA was detected using SuperSignal West Pico chemiluminescent substrate (Pierce). The int ...
... membrane was incubated for 2 h with PIA-specific antiserum (generously provided by Michael Otto), which was followed by a 2-h incubation with an antirabbit immunoglobulin G peroxidase conjugate. The presence of PIA was detected using SuperSignal West Pico chemiluminescent substrate (Pierce). The int ...
as Adobe PDF - Edinburgh Research Explorer
... design. The green arrow indicates Biniou’s continuous expression from stage 10 (∼6 h after egg laying) to the end of embryogenesis. ChIP-on-chip experiments were conducted at four consecutive 2-h time points (dark-blue bars). This was complemented by expression profiling of biniou loss-of-function a ...
... design. The green arrow indicates Biniou’s continuous expression from stage 10 (∼6 h after egg laying) to the end of embryogenesis. ChIP-on-chip experiments were conducted at four consecutive 2-h time points (dark-blue bars). This was complemented by expression profiling of biniou loss-of-function a ...
A High Density Integrated Genetic Linkage Map of Soybean and the
... tested produced robust STS that contained at least one SNP. The 3682 SNP-containing STS contained a total of 13,042 SNPs for an average of 3.5 SNPs per polymorphic STS. These SNPs were combined with the 5551 SNPs discovered by Choi et al. (2007) to design two Illumina custom 1536 SNP GoldenGate assa ...
... tested produced robust STS that contained at least one SNP. The 3682 SNP-containing STS contained a total of 13,042 SNPs for an average of 3.5 SNPs per polymorphic STS. These SNPs were combined with the 5551 SNPs discovered by Choi et al. (2007) to design two Illumina custom 1536 SNP GoldenGate assa ...
Left-right axis asymmetry determining human Cryptic gene is
... developing vertebrate-embryo. A number of factors like maternally deposited molecules have emerged essential in initiating the specification of the axis; the downstream events, however, are regulated by signal-transduction and gene-expression changes identifying which remains a crucial challenge. Th ...
... developing vertebrate-embryo. A number of factors like maternally deposited molecules have emerged essential in initiating the specification of the axis; the downstream events, however, are regulated by signal-transduction and gene-expression changes identifying which remains a crucial challenge. Th ...
Biochemistry and biosynthesis of insect pigments
... cyclization of linear polyketides via successive condensation of simple carboxylic acid metabolites and occur in two major Superfamilies of Hemiptera, the Coccoidea and Aphidoidae, respectively. Ommochromes, tetrapyrroles and melanins are derived from different amino acid precursors, tryptophan, gly ...
... cyclization of linear polyketides via successive condensation of simple carboxylic acid metabolites and occur in two major Superfamilies of Hemiptera, the Coccoidea and Aphidoidae, respectively. Ommochromes, tetrapyrroles and melanins are derived from different amino acid precursors, tryptophan, gly ...
Direct control of shoot meristem activity by a cytokinin
... wide range of organisms including bacteria, but their functions have not been assigned. Curiously, in some soil phytopathogenic bacteria such as Agrobacterium rhizogenes17 and Rhodococcus fascians18, the LOG homologues (riorf52 and fas ORF6, respectively) are located adjacent to the gene for adenosi ...
... wide range of organisms including bacteria, but their functions have not been assigned. Curiously, in some soil phytopathogenic bacteria such as Agrobacterium rhizogenes17 and Rhodococcus fascians18, the LOG homologues (riorf52 and fas ORF6, respectively) are located adjacent to the gene for adenosi ...
Molecular insights into mitochondrial transcription and its
... been reconstituted in vitro, a full molecular understanding is still missing. Initiation of mitochondrial DNA replication is believed to be primed by transcription prematurely terminated at a sequence known as CSBII. The mechanisms of replication initiation have however not been fully defined. In th ...
... been reconstituted in vitro, a full molecular understanding is still missing. Initiation of mitochondrial DNA replication is believed to be primed by transcription prematurely terminated at a sequence known as CSBII. The mechanisms of replication initiation have however not been fully defined. In th ...
Reviews Sex linked recessive dystonia parkinsonism of Panay
... tricks alleviated the movements. Table 2 correlates the initial involvement and duration of illness and the final status of their dystonia, showing the spread and progression of XDP depicting the natural history. Table 2 shows that generalisation occurs as early as the first two years of illness, no ...
... tricks alleviated the movements. Table 2 correlates the initial involvement and duration of illness and the final status of their dystonia, showing the spread and progression of XDP depicting the natural history. Table 2 shows that generalisation occurs as early as the first two years of illness, no ...
New and Redesigned pRS Plasmid Shuttle Vectors for Genetic
... ABSTRACT We have constructed a set of 42 plasmid shuttle vectors based on the widely used pRS series for use in the budding yeast Saccharomyces cerevisiae and the bacterium Escherichia coli. This set of pRSII plasmids includes new shuttle vectors that can be used with histidine and adenine auxotroph ...
... ABSTRACT We have constructed a set of 42 plasmid shuttle vectors based on the widely used pRS series for use in the budding yeast Saccharomyces cerevisiae and the bacterium Escherichia coli. This set of pRSII plasmids includes new shuttle vectors that can be used with histidine and adenine auxotroph ...
Youngson and Whitelaw, 2008
... To ensure the totipotency of the zygote and to prevent perpetuation of abnormal epigenetic states, most gene regulatory, i.e., epigenetic, information is not transferred between generations. Several mechanisms have evolved to erase the marks, including germline and somatic reprogramming of DNA methy ...
... To ensure the totipotency of the zygote and to prevent perpetuation of abnormal epigenetic states, most gene regulatory, i.e., epigenetic, information is not transferred between generations. Several mechanisms have evolved to erase the marks, including germline and somatic reprogramming of DNA methy ...
Red cell pyruvate kinase deficiency: molecular and clinical aspects
... the product pyruvate are involved in a number of energetic and biosynthetic pathways and the tight regulation of PK activity has been shown to be of great importance not only for glycolysis itself, but also for the entire cellular metabolism. Thus, one of the main features of this enzyme is its allo ...
... the product pyruvate are involved in a number of energetic and biosynthetic pathways and the tight regulation of PK activity has been shown to be of great importance not only for glycolysis itself, but also for the entire cellular metabolism. Thus, one of the main features of this enzyme is its allo ...
20071217161016301
... set to see if it yielded a different tree than that seen at the nucleotide level. ...
... set to see if it yielded a different tree than that seen at the nucleotide level. ...
Conjugative plasmids: vessels of the communal gene pool
... usually occur within the cytoplasm of a cell and therefore constitute intracellular movement. In describing the communal pool, one can therefore distinguish between: (i) translocative elements, described earlier, that enable the rearrangement, movement and accretion of genes; (ii) operative elements ...
... usually occur within the cytoplasm of a cell and therefore constitute intracellular movement. In describing the communal pool, one can therefore distinguish between: (i) translocative elements, described earlier, that enable the rearrangement, movement and accretion of genes; (ii) operative elements ...
"RNA Interference in Caenorhabditis elegans".
... et al., 2000). In the microinjection and soaking protocols, in vitro preparations of dsRNA are delivered mechanically using a needle to inject dsRNA directly into the body or passively by soaking worms in dsRNA solution. In the feeding protocols, dsRNA is transcribed in E. coli and ingested by anima ...
... et al., 2000). In the microinjection and soaking protocols, in vitro preparations of dsRNA are delivered mechanically using a needle to inject dsRNA directly into the body or passively by soaking worms in dsRNA solution. In the feeding protocols, dsRNA is transcribed in E. coli and ingested by anima ...
metabolic core - Chair of Computational Biology
... d, The distribution of experimentally determined fluxes from the central metabolism of E. coli shows power-law behaviour as well, with a best fit to P(v) v- with = 1. Both computed and experimental flux distribution show wide spectrum of fluxes. ...
... d, The distribution of experimentally determined fluxes from the central metabolism of E. coli shows power-law behaviour as well, with a best fit to P(v) v- with = 1. Both computed and experimental flux distribution show wide spectrum of fluxes. ...
Conjugative plasmids: vessels of the communal gene pool
... usually occur within the cytoplasm of a cell and therefore constitute intracellular movement. In describing the communal pool, one can therefore distinguish between: (i) translocative elements, described earlier, that enable the rearrangement, movement and accretion of genes; (ii) operative elements ...
... usually occur within the cytoplasm of a cell and therefore constitute intracellular movement. In describing the communal pool, one can therefore distinguish between: (i) translocative elements, described earlier, that enable the rearrangement, movement and accretion of genes; (ii) operative elements ...
The genetics of diabetes mellitus
... Excepting DR α , all the other genes are expressed. The β genes are known to be highly polymorphic 10 . The number of polymorphic alleles range between 18 (HLA-C) to 72 (HLA-DR), which gives several million possible haplotypes. For example, there are several loci at DR β region, such as DRB1, DRB2, ...
... Excepting DR α , all the other genes are expressed. The β genes are known to be highly polymorphic 10 . The number of polymorphic alleles range between 18 (HLA-C) to 72 (HLA-DR), which gives several million possible haplotypes. For example, there are several loci at DR β region, such as DRB1, DRB2, ...
Multiple Roles of the Y Chromosome in the Biology of
... on the mouse B2 transcript involved in heat-shock-induced transcription inhibition[28,29]. In general, the Y chromosome of most animals shows two features: (1) it harbors and accumulates by various means, mainly transposition and chromosome translocations, genes involved in male fertility[30]; and ( ...
... on the mouse B2 transcript involved in heat-shock-induced transcription inhibition[28,29]. In general, the Y chromosome of most animals shows two features: (1) it harbors and accumulates by various means, mainly transposition and chromosome translocations, genes involved in male fertility[30]; and ( ...
Reproductive barriers in indica-japonica rice
... of total DEGs) in “biotic stress” and “abiotic stress” and genes in various functional ...
... of total DEGs) in “biotic stress” and “abiotic stress” and genes in various functional ...
Functional Interactions of Genes Mediating Convergent Extension
... anterior-median tissue exhibiting abnormal pax gene expression differentiates as retina, resulting in the formation of a single cyclopic eye in the mutant embryos. Ectopic expression of shh, twhh, Indian hedgehog (Ihh), or a dominant negative form of protein kinase A leads to the reduction of pax6 e ...
... anterior-median tissue exhibiting abnormal pax gene expression differentiates as retina, resulting in the formation of a single cyclopic eye in the mutant embryos. Ectopic expression of shh, twhh, Indian hedgehog (Ihh), or a dominant negative form of protein kinase A leads to the reduction of pax6 e ...
Lesson Overview
... How do alleles segregate when more than one gene is involved? The principle of independent assortment states that genes for different traits can segregate independently during the formation of gametes. ...
... How do alleles segregate when more than one gene is involved? The principle of independent assortment states that genes for different traits can segregate independently during the formation of gametes. ...
CFTR mutation classifications toward genotype
... CF with milder single-organ phenotypes, this is not always the case. Whether the phenotype is "severe/mild" or "multi/single-organ" is not intrinsically linked to the classic or non-classic CF diagnosis. These are subjective terms, which should be used for descriptive purposes exclusively. The great ...
... CF with milder single-organ phenotypes, this is not always the case. Whether the phenotype is "severe/mild" or "multi/single-organ" is not intrinsically linked to the classic or non-classic CF diagnosis. These are subjective terms, which should be used for descriptive purposes exclusively. The great ...
Glutathione production by efficient ATP
... in permeable cells, termed as the ‘Permeable Cell Assay’ (Hara & Mori, 2006), and have systematically constructed a genome-wide single-gene deletion Escherichia coli K-12 library, termed as the ‘Keio Collection’ (Baba et al., 2006). A combination of the Permeable Cell Assay and the Keio Collection m ...
... in permeable cells, termed as the ‘Permeable Cell Assay’ (Hara & Mori, 2006), and have systematically constructed a genome-wide single-gene deletion Escherichia coli K-12 library, termed as the ‘Keio Collection’ (Baba et al., 2006). A combination of the Permeable Cell Assay and the Keio Collection m ...
RNA-Seq

RNA-seq (RNA sequencing), also called whole transcriptome shotgun sequencing (WTSS), is a technology that uses the capabilities of next-generation sequencing to reveal a snapshot of RNA presence and quantity from a genome at a given moment in time.