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Principles of Biology Lake Tahoe Community College
Principles of Biology Lake Tahoe Community College

... 6. DNA packing tends to prevent transcription and translation B. In female mammals, one x chromosome is inactivated in each cell 1. early in embryonic development. C. control of eukaryotic transcription 1. eukaryotes have transcription factors D. Eukaryotic RNA may be spliced in more than one way E. ...
CHAPTER 19 -- EUKARYOTIC GENE EXPRESSION YOU MUST
CHAPTER 19 -- EUKARYOTIC GENE EXPRESSION YOU MUST

...  The impact of DNA methylation and histone acetylation on gene expression  The role of oncogenes, proto-oncogenes, and tumor suppressor genes in cancer.  The various stages of the protein making process where genes or their products can be turned on or off.  That gene regulation can result in ce ...
MicroArray -- Data Analysis
MicroArray -- Data Analysis

... are it is of interestyou to characterize time series). (co-regulation) Reverse Engineering: the biological status of cells, interested in the subset of Hence, as a hypothesis, genes Using expression data to e.g. thewhich severeness of showing tumor of unknown function • patterns of expression genes ...
Lesson Plan
Lesson Plan

... Test October 29/30th, 2014 ...
Mosaic Analysis
Mosaic Analysis

... Goal: to measure RNA levels of all genes in genome RNA levels vary with the following: ...
jeopardy honors DNA 12-1 thru 12-4 only
jeopardy honors DNA 12-1 thru 12-4 only

... Helicase breaks these bonds between these molecules. ...
Chapter 8b
Chapter 8b

... Eukaryotes utilize transcription factors or alternate splicing of exons Expression may be regulated at translation level Unsure of regulation of expression in archaea  May be more similar to eukaryotes than bacteria ...
Gene Regulation
Gene Regulation

... transcription and translation. Best time to control gene expression is at transcription, before a mRNA is produced. Regulation in Prokaryotes Two types of regulation in prokaryotes: 1. Inducible - Turn on production of a protein only when it is needed. 2. Repressible - turn off production of a prote ...
The RNA World
The RNA World

... RNAi for analysis of gene function and as therapeutic - duplexes of 21-nt small interfering RNAs (siRNAs) - guide sequence-specific degradation of the homologous mRNA - degradation of targeted mRNAs, "knock-down" - targeting of essential genes causes growth arrest or triggers apoptosis ...
The RNA World
The RNA World

... RNAi for analysis of gene function and as therapeutic - duplexes of 21-nt small interfering RNAs (siRNAs) - guide sequence-specific degradation of the homologous mRNA - degradation of targeted mRNAs, "knock-down" - targeting of essential genes causes growth arrest or triggers apoptosis ...
Presentazione di PowerPoint
Presentazione di PowerPoint

... also promised to publish their findings in accordance with the terms of the 1996 "Bermuda Statement," ...
Gene co-option
Gene co-option

... What explains Darwin’s finches? • Difference in gene expression (high, moderate, or low) for two genes. What are the possible mechanisms? – Switch? – Gene duplication? – Epigenetics? ...
Unit 3 Concepts Study Guide
Unit 3 Concepts Study Guide

... 3. DNA microarrays measure the amount of mRNA for genes that is present in a cell sample. - Goal: to determine which genes are “turned on” and “turned off”- gene expression. - Results: Red means cancer cell expression; green means healthy cell expression; yellow means both are expressed; black means ...
Gene Section SRSF3 (serine/arginine rich splicing factor 3) -
Gene Section SRSF3 (serine/arginine rich splicing factor 3) -

... keratinocytes in the basal and parabasal layers, but drops significantly in terminally differentiated keratinocytes in the superficial layers of the cervix or skin. In general, normal cells like muscle or nerve cells have no or little expression of SRSF3. In contrast, malignant tumor cells express r ...
CALL FOR PROPOSALS 2008
CALL FOR PROPOSALS 2008

... suggest the technology to be used, but the final decision on technology choice will be made by France Génomique. In the case of de novo sequencing, indicate the need for finishing. Because of the high pace of evolution of New Sequencing Technologies, we will not generally propose manual finishing of ...
2007.6. JW
2007.6. JW

... The HERV-M derived transcripts (variant 1-3) were expressed in various human tissues, whereas it was not detected in crab-eating monkey and squirrel monkey tissues by RT-PCR amplification. ...
Name of structure?
Name of structure?

... Did you know that the genetic code is shared by all organisms? All 20 amino acids are common to all living systems ...
gene and epigenetic expression patterns of same-genome
gene and epigenetic expression patterns of same-genome

... Peaks defining where DNA was bound to the histone marks of interest (H3K4me3 and H3K9ac) were identified and quality scored. Using the UCSC genome browser it was observed that treatment B and A+B had very similar peak shifts, which differed from the control group in several important genic regions. ...
The aim of the thesis was to characterize chosen expression vectors
The aim of the thesis was to characterize chosen expression vectors

... their use in studies of promoter activity control by sigma factors of RNA polymerase. Different properties of these vectors (level of expression of the cloned gene, leaky expression without inducer, dependence of expression level on inducer concentration and cell population homogeneity) were found b ...
Term: SPRING 2000 - Washington University in St. Louis
Term: SPRING 2000 - Washington University in St. Louis

... Students will be involved in a ‘dry bench’ laboratory where they explore the structure of eukaryotic genomes. Student teams will be assigned ‘chunks’ of DNA (~50 kb) known as fosmids to annotate (discover new genes) by the end of the semester under the tutelage of faculty and upper division biology ...
A Nucleotide Consists of what three parts?
A Nucleotide Consists of what three parts?

... A mutation is  A permanent change in DNA ...
Microarrays
Microarrays

... To study gene expression, mRNA is isolated from the cells of interest and converted into labeled cDNA. This cDNA is then washed over a microarray carrying features representing all the genes that could possibly be expressed in those cells. If hybridization occurs to a certain feature, it means the g ...
RNA synthesis and processing
RNA synthesis and processing

... What are genes? As we defined previously, a gene is any DNA sequence that is transcribed into RNA. Why does a cell need to make these RNAs? Cell needs mRNA because they serve as the templates for protein synthesis. Cell needs rRNA and tRNA's because they are critically involved in protein synthesis. ...
Protein Synth Notes GO New
Protein Synth Notes GO New

... We look the way we do because _____________ build the _____________ which become our __________. ______________________ makes _________________. ____________ tells _____________ which __________________ to make. Therefore, __________ determines Central Dogma of Molecular Biology: __________ → ______ ...
Isolation of HSV-1 Small RNA Interference Molecules in Latently
Isolation of HSV-1 Small RNA Interference Molecules in Latently

... The herpes simplex virus type I (HSV-1) becomes dormant once infecting its host in sensory neurons of the trigeminal ganglia (TG). However, how HSV-1 establishes life-long latency is yet to be understood. Studies have shown all organisms have some type of counteractive mechanism towards their host’s ...
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RNA-Seq



RNA-seq (RNA sequencing), also called whole transcriptome shotgun sequencing (WTSS), is a technology that uses the capabilities of next-generation sequencing to reveal a snapshot of RNA presence and quantity from a genome at a given moment in time.
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