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Presentation
Presentation

... • Created a Receiver-Operator (ROC) Curve (True positive rate vs. False ...
Central Dogma
Central Dogma

... 2. If the number of bacteria continued to increase at the same rate as the pond continued to warm, what would the measurement be at 30 degrees? A. 400 B. 640 C. 860 D. 1270 3. Based on the information presented, the number of which of the following substances is not determined by the pond's temperat ...
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Document

... • ~97-98% of the transcriptional output of the human genome is ncRNA – Introns – Transfer RNAs (tRNA) • ~ 500 tRNA genes in human genome – Ribosomal RNAs • Tandem arrays on several chromosomes • 150-200 copies of 28S – 5.8S – 18S cluster • 200-300 copies of 5S cluster ...
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Yeast Cell-Cycle Regulation Network inference

... • Problem1: Cluster using the whole data set always did not show good results because of the noise and the complex network. • Problem2: How to select a cluster method. ...
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I. Biology (35 points total) The following questions cover some of the

... An eukaryotic gene starts with a promoter, where various transcription factors and RNA polymerase bind to initiate transcription. It’s usually upstream of the exon; Exon is the coding sequence of the gene; Some times, a gene has introns, which are intervening sequences between exons and are spiced o ...
molecular scissors to study gene function Marta Oliveira
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... Targeting and cutting DNA is possible and allows the modification of model organism genome. In this case, the CRISPR-Cas technique was used to silence two key genes in kidney and vasculature development in zebrafish. The term CRISPR (clustered regularly interspaced palindromic repeats) is used to de ...
Genetic disease and the genome
Genetic disease and the genome

... identification of mouse homologues of genes deleted in Cri du Chat. A large focus of the laboratory is the characterization of the Treacher Collins syndrome (TCOF1) gene. Bioinformatics information is important in every step of human disease gene identification and characterization. Identification o ...
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... oligonucleotides obtained by partial digestion of RNA. One important development described in this paper was the use of 32Plabelled RNA of high specific activity. This made it possible to work on a small scale and to use two-dimensional ‘paper’ fractionation techniques, which had high resolving powe ...
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... Primary transcripts of microRNAs can produce multiple functional products. For example, around a half of mammalian microRNAs are located in introns of protein-coding genes. In these cases, the microRNA and the protein-coding mRNA are processed from the same transcript. Similarly, around a third of a ...
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... – Digest BAC-inserted clonal chunks of DNA into small fragments. – Sequence small fragments. – Stitch together BAC clones to assemble sequence. – Assemble genome sequence from BAC clone sequences, using clone-based physical map. ...
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... The organization of the rRNA genes of Schistosoma mansoni has been determined by Southern blot analysis of genomic DNA digested with restriction enzymes, by isolation of the entire repeat on a single fragment of about 11 kilobase pairs from a genomic DNA library constructed in bacteriophage lambda a ...
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... decisions in a timely manner. However, negative FISH results necessitate sequential testing for alternate candidate targets, increasing the amount of lab work, and the total time required to yield meaningful answers. In these cases next-generation sequencing (NGS) complements FISH well. Performing b ...
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... polymerase chain reaction cycled sequencing using internal oligonucleotides to prime either on subclones of positive fragments or directly on the cosmid DNA. The size of each of the introns was determined by estimating their sizes by PCR between the exons. This analysis revealed that the sulphamidas ...
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... related endangered animals are not mated together. • To establish how closely related different seed stocks are • To place a suspect at the crime scene in forensic science. ...
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... A candidate tumor suppressor gene. Ndrg2 expressed much higher in normal tissues than the tumors (brain, liver, pancreas tissues etc). Overexpression of ndrg2 can inhibit the proliferation of glioblastoma U373 and U138 cells. NDRG2 upregulation is associated with disease pathogenesis in the human br ...
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... Considering the location of genetic material in the interphase nucleus, certain chromosomal territories appear to exist. Specifically, A. each chromosome appears to occupy a discrete domain. B. gene-poor regions of chromosomes are located outside the nucleus, whereas gene-rich regions are located in ...
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... tive splicing, discovered in viruses in 1977 (see genes. But many do not. “It’s somewhat revolu‘Hard to track’, overleaf). Most of the DNA tionary,” says Gingeras’s colleague Phillip sequences describing proteins in humans have a Kapranov. “We’ve come to the realization that modular arrangement in w ...
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RNA-Seq



RNA-seq (RNA sequencing), also called whole transcriptome shotgun sequencing (WTSS), is a technology that uses the capabilities of next-generation sequencing to reveal a snapshot of RNA presence and quantity from a genome at a given moment in time.
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