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Science 103: Outline 17
Science 103: Outline 17

... Gene (DNA)  (b) Functions (i) (ii) (c) Location (d) Process A single-stranded RNA copy of the DNA is made by RNA polymerase: (i) RNA pol binds to and moves down the DNA, separating the strands. (ii) As it goes, it pairs complementary nucleotides with ONE strand, the template strand (*NOT coding str ...
Document
Document

... _____ 1. A gene is expressed when it is a. present in the genome of an individual. b. prevented from interacting with RNA polymerase. c. transcribed into mRNA. d. duplicated during the replication of DNA. _____ 2. In the lac operon of E. coli, lactose functions as a. a promoter. b.an operator. c. a ...
Lecture 1, Part I
Lecture 1, Part I

... • Genes comprise only about 2% of the human genome; the rest consists of non-coding regions, whose functions may include providing chromosomal structural integrity and regulating when, where, and in what quantity proteins are made (regulatory regions). • The terms exon and intron refer to coding (tr ...
CHAPTER OUTLINE
CHAPTER OUTLINE

... Elongation is the protein synthesis step in which a polypeptide increases in length one amino acid at a time. Termination During termination, the polypeptide and the assembled components that carried out protein synthesis are separated from one another. Review of Gene Expression A gene is expressed ...
From Gene to Protein
From Gene to Protein

... •Transfer of Information from DNA to RNA • Transcription: mRNA production, or the synthesis of other types of RNA • Short segment of DNA (gene) is transcribed into mRNA for movement out of the nucleus to the ribosome • DNA is used as a template to make a complementary piece of “messenger RNA”. This ...
Chapter 12.5
Chapter 12.5

... • THE PROMOTER REGION IS WHERE RNA POLYMERASE CAN ATTACH AND START TRANSCRIPTION. • THERE IS ANOTHER REGION CALLED THE OPERATOR. THIS CONTAINS A PROTEINS CALLED A LAC REPRESSOR. IF THE LAC REPRESSOR IS ATTACHED TO THE DNA THE GENE IS TURNED OFF. • THE REPRESSOR WILL BE ON DNA UNLESS LACTOSE IS PRESE ...
Gene expression and DNA microarrays
Gene expression and DNA microarrays

... • The amount of RNA produced from a gene. • Level of RNA produced from a gene is controlled by: – Transcription – Stability/Degradation ...
Review Questions
Review Questions

... DNA, the recipe for making proteins, never leaves the nucleus (nucleoid region in bacteria). Yet all the protein-making machinery is located out in the cytoplasm. So how does the information get to the cytoplasm? DNA is transcribed into messenger RNA. 2. What is a transcript? A transcript is not a c ...
Lecture 1 Introduction to Bioinformatics
Lecture 1 Introduction to Bioinformatics

... –  at one end, the terminal carbon atom in the backbone is the 5’ carbon atom of the terminal sugar –  at the other end, the terminal carbon atom is the 3’ carbon atom of the terminal sugar •  therefore we can talk about the 5’ and the 3’ ends of a DNA strand •  in a double helix, the strands are an ...
Presentation
Presentation

... unless an inducer is present  Inducer attaches to the repressor and causes it to move so that transcription can occur ...
RNA Interference Provides New Approach for Finding Cancer Genes
RNA Interference Provides New Approach for Finding Cancer Genes

... In the past, researchers used this method to study the effects of turning off one particular gene. But to study the effects of thousands of genes, researchers had to run thousands of separate experiments with thousands of plates of cells. Instead, Hannon and Elledge developed a method to track a div ...
Translation - CS
Translation - CS

...  Computational ...
DNA versus RNA Notes File
DNA versus RNA Notes File

... deoxyribose. ...
ppt for
ppt for

... protein-coding genes ...
Chapter 4 Section 4 – The DNA Connection
Chapter 4 Section 4 – The DNA Connection

... addition to the new protein chain. ...
National Research Program
National Research Program

... RNA is produced when a single-stranded, complementary ‘copy’ of a gene’s DNA sequence is transcribed. Post-transcription, RNA editing can occur. Editing involves changing, adding or deleting the nucleotides that make up the RNA’s coding sequence. Editing can dramatically alter the proteins produced ...
The entire human genome consists of 23 pairs of chromosomes
The entire human genome consists of 23 pairs of chromosomes

... information of thousands of healthy individuals with a database that reveals which genetic elements are active during prenatal brain development. They used this to identify a set of exons that are highly expressed in the brain but rarely vary from person to person. MUTATION RATE HIGH ...
RUNX1-RUNX1T1 pre
RUNX1-RUNX1T1 pre

... Sequence features are not equal in importance for the prediction of the ECI value ...
Genes and How they work!
Genes and How they work!

... forming a 5’ cap. Protects end from degradation by nucleases and phosphotases. • 3’ end contain AAUAAA, poly A polymerase adds about 250 A’s to 3’ end  long A tail. Needed to prevent degradation. ...
Slide 1
Slide 1

... qRT-PCR data with weight loss are shown. The PCR and array data were scaled to make them comparable and each dataset is plotted against % weight loss. For each dataset the least squares line is also shown. P-values for the correlations ranged from 0.03 to below 0.01. All genes excepts SGK1 validated ...
Study Guide Unit 4 - Mrs. Wolodkowicz`s Biological Realm
Study Guide Unit 4 - Mrs. Wolodkowicz`s Biological Realm

...  write the definitions for DNA & RNA, transcription & translation, autosome, & sex linkage.  the components of DNA  the nitrogen bases & their complementary base pairs in DNA & RNA  functions of tRNA & mRNA  the laws of segregation & independent assortment  the terms: dominant, recessive, geno ...
dna, data, deği̇şi̇m
dna, data, deği̇şi̇m

... 7. Internationalisation of Healthcare ...
Brian Yu - Comparison of Microarrayc and RNA-Seq Analysis Methods for Single Cell Transcriptiomics
Brian Yu - Comparison of Microarrayc and RNA-Seq Analysis Methods for Single Cell Transcriptiomics

... Currently, gene expression microarrays and RNA-seq are two popular ways of extracting single cell transcriptome data. Both methods allow high throughput analysis of many cells and gene targets. Developed in the 1990s, high density microarrays are more mature than deep sequencing technologies. With a ...
Powerpoint slides - Berkeley Statistics
Powerpoint slides - Berkeley Statistics

... Idea: measure the amount of mRNA to see which genes are being expressed in (used by) the cell. Measuring protein might be better, but is currently harder. ...
DNA, RNA, Genes, Chromosomes
DNA, RNA, Genes, Chromosomes

... Genes are the biological units of heredity. They determine obvious traits, such as hair and eye color, as well as more subtle characteristics, such as the oxygencarrying ability of the blood. Complex traits, such as IQ and physical strength, may be shaped by the interaction of a number of different ...
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RNA-Seq



RNA-seq (RNA sequencing), also called whole transcriptome shotgun sequencing (WTSS), is a technology that uses the capabilities of next-generation sequencing to reveal a snapshot of RNA presence and quantity from a genome at a given moment in time.
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