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... A string of ribosomes carrying out multiple translation on the same mRNA strand is called a polyribosome ...
Making RNA in other ways
Making RNA in other ways

... the existence of introns • Introns may become copied into DNA or directly inserted into DNA as RNA – The insertion is sequence specific – So if cells fuse (as during fertilization) a retrotransposon can copy an intron from a gene on one chromosome and insert it into the same gene at the same place o ...
Gene Q
Gene Q

... PCR primers were used to amplify Sperm number DNA isolated from one man's ...
Horizontal Gene Transfer
Horizontal Gene Transfer

... Common ancestor may have been a community of cell lineage evolving as a whole rather than a single cell lineage Muddles concept of monophyletic groups ...
Identification of Microorganisms Using PCR
Identification of Microorganisms Using PCR

... the ribosomes of prokaryotes and eukaryotes, the sequences of the rRNA molecules (and their corresponding rDNA genes) from all sources contain regions that are very similar, allowing the alignment and comparison of these sequences. Further, the gene is small enough to be easily sequenced and large e ...
2.5 Genetics - Elaine Galvin
2.5 Genetics - Elaine Galvin

... (Matching) RNA production (notion of both DNA and RNA must be given) The process of making a protein using the mRNA code a template A haploid sex cell which is capable of fusion The fusion of 2[haploid] gametes to form a [diploid] zygote An alternative form of a gene Has identical alleles [for a tra ...
Review - Molecular and Cell Biology
Review - Molecular and Cell Biology

... most mutations are spontaneous and rare DNA repair mechanisms eliminate most mutations mutagens such as Xrays or chemicals like EMS can greatly increase the mutation rate, and are essential tools for experimental isolation of mutants Mutations can affect the DNA sequence of genes in a variety of way ...
rubric
rubric

... ...
Genetics Review
Genetics Review

... A. Their products act as transcription factors. B. They have no counterparts in animals other than Drosophila. C. Their products are all synthesized prior to fertilization. D. They act independently of other positional information. E. They apparently can be activated and inactivated at any time of t ...
Topic 3 The Chemistry of Life - wfs
Topic 3 The Chemistry of Life - wfs

... 2. Helicase is the enzyme that breaks the hydrogen bonds to allow the unwinding. 3. The exposed bases of each strand are then paired with an available nucleotide by complementary base pairing. The result is two strands where only one was first present. 4. DNA polymerase is an enzyme that allows the ...
Two Epigenetic Mechanisms
Two Epigenetic Mechanisms

... Refers to changes in gene expression caused by mechanisms other than changes in the underlying DNA sequence. Enables a cell/organism to respond to its dynamic external environment during development and throughout life! Epigenetic changes to the genome can be inherited if these changes occur in cell ...
mirna target prediction
mirna target prediction

... • miRNAs tend to have conserved function and targets • Can use cross species conservation to improve prediction – high confidence targets • Lower conservation in 3’ UTRs but functional motifs (e.g. target sites) are strongly conserved • Drawback: not all targets are conserved! The Genome Analysis Ce ...
Secondary structures
Secondary structures

... Translation is performed on ribosomes Replication is carried out by DNA polymerase Reverse transcriptase copies RNA into DNA ...
Central Dogma! - Cloudfront.net
Central Dogma! - Cloudfront.net

... of terminator (nucleotide sequence) • In eukaryotes: polymerase continues transcription after pre-mRNA is cut  polymerase eventually falls off DNA ...
The Effects of Predictive Genetic Testing on the - Antioch Co-op
The Effects of Predictive Genetic Testing on the - Antioch Co-op

... of the double stranded DNA template into two single stranded molecules Annealing - The oligonucleotide primers anneal to or find their complementary sequences on the two single-stranded template strands of DNA. These act as primers for taq polymerase. All of this is done at 60℃ Extension - Taq polym ...
Document
Document

... – can detect some genes related to an increased risk of cancer – can detect some genes known to cause genetic disorders ...
9.6 Genetic Screening and Gene Therapy KEY CONCEPT treatments.
9.6 Genetic Screening and Gene Therapy KEY CONCEPT treatments.

... – can detect some genes related to an increased risk of cancer – can detect some genes known to cause genetic disorders ...
Chapter 10 Workbook Notes
Chapter 10 Workbook Notes

... Transcription factors bind to DNA and regulate transcription. Activators are a type of transcription factor that binds to enhancers. Other transcription factors bind to the promoter in eukaryotic genes and help arrange RNA polymerase in the correct position. A loop in the DNA allows the activator b ...
DNA & RNA
DNA & RNA

...  RNA molecules that carry copies of these instructions are known as mRNA  Proteins are assembled on the ribosomes. Ribosomes are made up of several dozen proteins as well as a form of RNA called rRNA  During protein construction, a third type of RNA molecule transfers each amino acid to the ribos ...
Gene Therapy: Using Viral and Non-Viral Vectors to Deliver Therapeutic Genes to the Human Body
Gene Therapy: Using Viral and Non-Viral Vectors to Deliver Therapeutic Genes to the Human Body

... env genes in retrovirus, and package in a cell with these genes Problem: Integrase enzyme inserts gene anywhere in genome – Can cause cancer – Use zinc finger nucleases or control sequence to direct integration site ...
RNA-Seq Sample Recommendations (Craig Praul, PSU and Caitlyn
RNA-Seq Sample Recommendations (Craig Praul, PSU and Caitlyn

... Investigators need to carefully choose their methods of tissue and cell isolation as the choice of these methods have a direct impact on the quality and quantity of RNA subsequently obtained. If possible total RNA purification should follow tissue/cell dissection immediately to prevent alterations i ...
Document
Document

... • A Gene is the fundamental physical and functional unit of heredity. A gene is an ordered sequence of nucleotides located in a particular position on a particular chromosome that encodes a specific functional product (i.e., a protein or RNA molecule). • A Genome is all the genetic material (DNA) in ...
BIOL 2416 Genetics
BIOL 2416 Genetics

... • Seeks to compare all or part of the genomes of two or more strains or species • Allows researchers to hone in on crucial genetic differences between strains/species/cell types. For example: – Using computer analysis: • May discover which gene(s) cause one virus to be more virulent than another • C ...
SI Worksheet 11
SI Worksheet 11

... e. ACU 7. A sequence of pictures of polypeptides synthesis shows a ribosome holding two transfer RNAs. One tRNA has a polypeptide chain attached to it, the other tRNA has a single amino acid attaches to it. What does the next picture show? a. the polypeptide chain moves over and bonds to the single ...
Epigenetics
Epigenetics

... • The second kind of mark, called histone modification, indirectly affects the DNA in your genome. • Histones are proteins which enable DNA's molecules to be wound up neatly into chromosomes inside the cell nucleus. • A variety of chemical tags can grab hold of the tails of histones, changing how t ...
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RNA-Seq



RNA-seq (RNA sequencing), also called whole transcriptome shotgun sequencing (WTSS), is a technology that uses the capabilities of next-generation sequencing to reveal a snapshot of RNA presence and quantity from a genome at a given moment in time.
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