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Microarray
Microarray

... A microarray is a solid support (such as a membrane or glass microscope slide) on which DNA of known sequence is deposited in a grid-like array. ...
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Antisense RNA

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... designed to hybridize with mRNAs and prevent translation.  Must hybridize to the target mRNA, be resistant to degradation, and be delivered into cells easily.  Usually 15-24 nucleotides long.  Can be designed from nearly any portion of the mRNA (5’ to 3’ ends of mRNAs, intron-exon boundaries, and ...
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... of the cell or exported out of the cell Figure 10.20 • Summary of transcription and translation Review: The flow of genetic information in the cell is DNA→RNA→protein • The sequence of codons in DNA spells out the primary structure of a polypeptide – Polypeptides form proteins that cells and organis ...
ppt - University of Connecticut
ppt - University of Connecticut

... Fig. 9(a) shows that in genes with more transcripts is more difficult to correctly reconstruct all transcripts. As a result Cufflinks performs better on genes with few transcripts since annotations are not used in it standard settings. DRUT has higher sensitivity on genes with 2 and 3 transcripts, b ...
Diapositiva 1
Diapositiva 1

... Pax6a  expressed in brain and retain the regulatory region for brain expression. Pax6b  expressed in developing pancreas has a downstream loss of brain elements, while upstream evolved to be pancreas specific. ...
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the efficient expression of a eukaryotic gene in a prokaryotic cell free

... more pronounced product at 17.5K comigrates with authentic TMV coat protein. Peptide mapping techniques confirm it to be T V coat protein with an extra N-terminal fmet residue as shown by differential labelling patterns. This product can be assembled into nucleoprotein particles. The structure of th ...
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Press release - Humangenetik

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Lecture 9 RNA world and emegence of complexity

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... Match the correct term with each definition or select the best answer for each question. 1. A series of codons from a single strand of DNA sequence which can be "read" in three different ways, depending on whether one starts at the first nucleotide position, the second or third Reading Frame (RF) Al ...
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Mr. Carpenter`s Biology RNA 10 Name

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Lecture Three: Genes and Inheritance
Lecture Three: Genes and Inheritance

... Carbohydrates - short term energy storage; structure of the organism (plants) Lipids - (also known as fats) - long term energy storage Nucleic Acids (DNA and RNA) - the instructions the cell uses to build proteins Proteins: The highly variable macromolecules that make each organism unique Structural ...
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... The appropriate transcription factors are needed for expression of inserted genome to begin U3 region is the binding site for a number of cellular transcription factors A TATA box is present upstream (U3/R segments) allowing transcription initiation to begin by RNA Pol II Transcription begins at the ...
Molecular genetics of gene expression
Molecular genetics of gene expression

... Figure 6.12 The genetic code gives rise to either overlapping or non-overlapping reading sequences. A codon consists of three consecutive nucleotides that code for an amino acid. The nucleotides in a codon may give rise to multiple amino acids depending on the reading frame. ...
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... polymorphisms? – Human gene nomenclature & designation by mutant phenotype (e.g., the “CF gene”) Molecular genetics: what are Southern, northern and western blots? what are RFLPs, microsatellites and SNPs and how are they used? what is PCR? what are microarrays? ...
Teacher PowerPoint - UNC Institute for the Environment
Teacher PowerPoint - UNC Institute for the Environment

... Refers to changes in gene expression caused by mechanisms other than changes in the underlying DNA sequence. Enables a cell/organism to respond to its dynamic external environment during development and throughout life! Epigenetic changes to the genome can be inherited if these changes occur in cell ...
Companion PowerPoint slide
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... Refers to changes in gene expression caused by mechanisms other than changes in the underlying DNA sequence. Enables a cell/organism to respond to its dynamic external environment during development and throughout life! Epigenetic changes to the genome can be inherited if these changes occur in cell ...
The logic of plant signalling
The logic of plant signalling

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RNA Detection and quantitation
RNA Detection and quantitation

... • mRNA or transcript levels are immensely informative of the state of a cell. • In general increases in the levels of a particular protein is reflected by increases in the corresponding mRNA ...
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RNA-Seq



RNA-seq (RNA sequencing), also called whole transcriptome shotgun sequencing (WTSS), is a technology that uses the capabilities of next-generation sequencing to reveal a snapshot of RNA presence and quantity from a genome at a given moment in time.
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